Standard name
Description Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0.06 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.89 0.91 0.96 0.94 0.91 0.85 0.87 0.86 0.86 0.72 0.83 0.96 0.97 0.96 0.18 0.19 0.14 0.66 0.74 0.63 0.78 0.78 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.38 0.29 0.35 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.13 0 0 0 0.06 0.12 0.07 0.17 0.11 0.36 0.17 0 0 0 0 0.05 0.06 0.14 0.06 0.13 0.1 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0.05 0.05 0 0 0.05 0 0 0 0 0.52 0.53 0.51 0.05 0.05 0.11 0.05 0.06 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.11 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 2 1 1 2 7 5
Bud 0 4 0 1 1 3 5 9 7 8 5 4 1 0 0 3 3 5 3 0 1 0 10 10
Bud Neck 1 2 2 14 28 13 20 32 13 30 3 10 8 4 2 4 2 3 0 0 0 0 1 5
Bud Site 0 0 0 1 0 0 0 3 0 0 3 1 0 0 0 9 4 9
Cell Periphery 0 1 1 1 2 4 3 2 2 2 1 0 2 1 0 0 0 0 1 0 1 1 4 7
Cytoplasm 161 112 78 282 451 471 549 556 411 411 247 307 362 514 565 61 34 45 131 95 65 188 383 462
Endoplasmic Reticulum 1 3 2 1 2 2 1 1 0 2 1 0 0 1 0 14 7 14 3 0 0 0 1 3
Endosome 0 0 0 1 0 1 2 0 0 2 1 0 1 1 8 124 52 112 8 2 2 2 4 6
Golgi 1 0 0 0 1 0 1 0 0 0 0 0 1 0 2 1 1 0 2 2 0 0 2 1
Mitochondria 25 17 0 1 4 29 74 45 79 55 123 64 0 0 0 10 9 20 27 8 13 24 28 29
Nucleus 0 0 1 0 2 3 10 9 7 6 4 3 1 0 0 13 10 14 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 26 16 25 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 4 1 10 0 0 0 0 0 1
SpindlePole 1 1 4 7 13 20 29 31 10 20 17 12 9 13 17 170 94 163 9 6 11 13 27 26
Vac/Vac Membrane 2 0 0 1 5 4 5 6 6 0 4 6 0 1 0 42 20 33 4 5 2 1 1 6
Unique Cell Count 180 126 86 295 481 517 643 636 476 479 342 369 377 530 589 330 178 318 200 129 104 243 489 588
Labelled Cell Count 192 142 89 310 511 552 699 694 535 536 410 407 385 535 595 482 254 454 200 129 104 243 489 588


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 3.0 4.6 4.3 4.7 3.9 3.5 4.0 3.5 3.4 3.3 3.5 4.8 5.0 5.1 5.9 6.1 5.9 4.4 4.6 4.9
Std Deviation (1e-4) 0.6 0.7 1.7 1.6 1.9 2.2 1.5 1.8 1.6 1.1 1.4 1.2 1.2 1.4 1.9 1.2 1.8 1.6 1.1 1.3 1.6
Intensity Change (Log2) -0.07 0.04 -0.24 -0.39 -0.18 -0.39 -0.42 -0.48 -0.4 0.06 0.13 0.16 0.37 0.41 0.37 -0.06 0.0 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.5 3.2 3.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cnn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available