Standard name | |
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Description | Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 7 | 5 |
Bud | 0 | 4 | 0 | 1 | 1 | 3 | 5 | 9 | 7 | 8 | 5 | 4 | 1 | 0 | 0 | 3 | 3 | 5 | 3 | 0 | 1 | 0 | 10 | 10 |
Bud Neck | 1 | 2 | 2 | 14 | 28 | 13 | 20 | 32 | 13 | 30 | 3 | 10 | 8 | 4 | 2 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 9 | 4 | 9 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 2 | 4 | 3 | 2 | 2 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 7 |
Cytoplasm | 161 | 112 | 78 | 282 | 451 | 471 | 549 | 556 | 411 | 411 | 247 | 307 | 362 | 514 | 565 | 61 | 34 | 45 | 131 | 95 | 65 | 188 | 383 | 462 |
Endoplasmic Reticulum | 1 | 3 | 2 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 14 | 7 | 14 | 3 | 0 | 0 | 0 | 1 | 3 |
Endosome | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 8 | 124 | 52 | 112 | 8 | 2 | 2 | 2 | 4 | 6 |
Golgi | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 2 | 1 |
Mitochondria | 25 | 17 | 0 | 1 | 4 | 29 | 74 | 45 | 79 | 55 | 123 | 64 | 0 | 0 | 0 | 10 | 9 | 20 | 27 | 8 | 13 | 24 | 28 | 29 |
Nucleus | 0 | 0 | 1 | 0 | 2 | 3 | 10 | 9 | 7 | 6 | 4 | 3 | 1 | 0 | 0 | 13 | 10 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 26 | 16 | 25 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 10 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 1 | 4 | 7 | 13 | 20 | 29 | 31 | 10 | 20 | 17 | 12 | 9 | 13 | 17 | 170 | 94 | 163 | 9 | 6 | 11 | 13 | 27 | 26 |
Vac/Vac Membrane | 2 | 0 | 0 | 1 | 5 | 4 | 5 | 6 | 6 | 0 | 4 | 6 | 0 | 1 | 0 | 42 | 20 | 33 | 4 | 5 | 2 | 1 | 1 | 6 |
Unique Cell Count | 180 | 126 | 86 | 295 | 481 | 517 | 643 | 636 | 476 | 479 | 342 | 369 | 377 | 530 | 589 | 330 | 178 | 318 | 200 | 129 | 104 | 243 | 489 | 588 |
Labelled Cell Count | 192 | 142 | 89 | 310 | 511 | 552 | 699 | 694 | 535 | 536 | 410 | 407 | 385 | 535 | 595 | 482 | 254 | 454 | 200 | 129 | 104 | 243 | 489 | 588 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 3.1 | 3.0 | 4.6 | 4.3 | 4.7 | 3.9 | 3.5 | 4.0 | 3.5 | 3.4 | 3.3 | 3.5 | 4.8 | 5.0 | 5.1 | 5.9 | 6.1 | 5.9 | 4.4 | 4.6 | 4.9 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.7 | 1.6 | 1.9 | 2.2 | 1.5 | 1.8 | 1.6 | 1.1 | 1.4 | 1.2 | 1.2 | 1.4 | 1.9 | 1.2 | 1.8 | 1.6 | 1.1 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.04 | -0.24 | -0.39 | -0.18 | -0.39 | -0.42 | -0.48 | -0.4 | 0.06 | 0.13 | 0.16 | 0.37 | 0.41 | 0.37 | -0.06 | 0.0 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.5 | 3.2 | 3.1 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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