Standard name | |
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Description | Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 7 | 5 |
Bud | 0 | 4 | 0 | 1 | 1 | 3 | 5 | 9 | 7 | 8 | 5 | 4 | 1 | 0 | 0 | 3 | 3 | 5 | 3 | 0 | 1 | 0 | 10 | 10 |
Bud Neck | 1 | 2 | 2 | 14 | 28 | 13 | 20 | 32 | 13 | 30 | 3 | 10 | 8 | 4 | 2 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 9 | 4 | 9 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 2 | 4 | 3 | 2 | 2 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 7 |
Cytoplasm | 161 | 112 | 78 | 282 | 451 | 471 | 549 | 556 | 411 | 411 | 247 | 307 | 362 | 514 | 565 | 61 | 34 | 45 | 131 | 95 | 65 | 188 | 383 | 462 |
Endoplasmic Reticulum | 1 | 3 | 2 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 14 | 7 | 14 | 3 | 0 | 0 | 0 | 1 | 3 |
Endosome | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 8 | 124 | 52 | 112 | 8 | 2 | 2 | 2 | 4 | 6 |
Golgi | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 2 | 1 |
Mitochondria | 25 | 17 | 0 | 1 | 4 | 29 | 74 | 45 | 79 | 55 | 123 | 64 | 0 | 0 | 0 | 10 | 9 | 20 | 27 | 8 | 13 | 24 | 28 | 29 |
Nucleus | 0 | 0 | 1 | 0 | 2 | 3 | 10 | 9 | 7 | 6 | 4 | 3 | 1 | 0 | 0 | 13 | 10 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 26 | 16 | 25 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 10 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 1 | 4 | 7 | 13 | 20 | 29 | 31 | 10 | 20 | 17 | 12 | 9 | 13 | 17 | 170 | 94 | 163 | 9 | 6 | 11 | 13 | 27 | 26 |
Vac/Vac Membrane | 2 | 0 | 0 | 1 | 5 | 4 | 5 | 6 | 6 | 0 | 4 | 6 | 0 | 1 | 0 | 42 | 20 | 33 | 4 | 5 | 2 | 1 | 1 | 6 |
Unique Cell Count | 180 | 126 | 86 | 295 | 481 | 517 | 643 | 636 | 476 | 479 | 342 | 369 | 377 | 530 | 589 | 330 | 178 | 318 | 200 | 129 | 104 | 243 | 489 | 588 |
Labelled Cell Count | 192 | 142 | 89 | 310 | 511 | 552 | 699 | 694 | 535 | 536 | 410 | 407 | 385 | 535 | 595 | 482 | 254 | 454 | 200 | 129 | 104 | 243 | 489 | 588 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | 3.0 | 4.6 | 4.3 | 4.7 | 3.9 | 3.5 | 4.0 | 3.5 | 3.4 | 3.3 | 3.5 | 4.8 | 5.0 | 5.1 | 5.9 | 6.1 | 5.9 | 4.4 | 4.6 | 4.9 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.7 | 1.6 | 1.9 | 2.2 | 1.5 | 1.8 | 1.6 | 1.1 | 1.4 | 1.2 | 1.2 | 1.4 | 1.9 | 1.2 | 1.8 | 1.6 | 1.1 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.04 | -0.24 | -0.39 | -0.18 | -0.39 | -0.42 | -0.48 | -0.4 | 0.06 | 0.13 | 0.16 | 0.37 | 0.41 | 0.37 | -0.06 | 0.0 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.5 | 3.2 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7794 | 0.4488 | 0.2998 | -0.017 | 1.0126 | 0.5287 | -1.3508 | -0.5551 | -0.4419 | -1.1014 | -0.7599 | -0.2955 | -1.0883 | -1.598 | -1.3778 | -1.6709 | -2.1948 | -1.8974 |
Actin | 0.0296 | 0.0023 | 0.0173 | 0.0009 | 0.0355 | 0.0021 | 0.0271 | 0.001 | 0.0128 | 0.032 | 0.0112 | 0.0074 | 0.0628 | 0.0012 | 0.0132 | 0.0028 | 0.0002 | 0.0014 |
Bud | 0.0024 | 0.0003 | 0.0007 | 0.0014 | 0.0013 | 0.0006 | 0.0016 | 0.0009 | 0.0006 | 0.0002 | 0.008 | 0.0006 | 0.0006 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 |
Bud Neck | 0.002 | 0.0002 | 0.0007 | 0.0053 | 0.001 | 0.0071 | 0.0023 | 0.0003 | 0.0009 | 0.0087 | 0.0007 | 0.0035 | 0.003 | 0.0003 | 0.0003 | 0.0004 | 0.001 | 0.0006 |
Bud Periphery | 0.0057 | 0.0003 | 0.0015 | 0.0033 | 0.0026 | 0.0033 | 0.0047 | 0.0021 | 0.0008 | 0.0005 | 0.0293 | 0.002 | 0.0007 | 0.0001 | 0.0005 | 0 | 0.0003 | 0 |
Bud Site | 0.0097 | 0.0021 | 0.0027 | 0.0185 | 0.003 | 0.0015 | 0.0102 | 0.0042 | 0.0024 | 0.0015 | 0.0023 | 0.0005 | 0.0096 | 0.0012 | 0.0027 | 0 | 0.0003 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 |
Cytoplasm | 0.0327 | 0.0498 | 0.0384 | 0.0438 | 0.0126 | 0.0485 | 0.0528 | 0.0466 | 0.0331 | 0.0867 | 0.0302 | 0.0641 | 0.0312 | 0.1109 | 0.0748 | 0.0111 | 0.1284 | 0.1592 |
Cytoplasmic Foci | 0.0802 | 0.0548 | 0.0658 | 0.0977 | 0.0987 | 0.0652 | 0.1116 | 0.0709 | 0.1027 | 0.0868 | 0.0637 | 0.0602 | 0.1699 | 0.1167 | 0.0818 | 0.0639 | 0.0544 | 0.0739 |
Eisosomes | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.001 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0062 | 0.0007 | 0.0012 | 0.0006 | 0.0044 | 0.0025 | 0.0016 | 0.0012 | 0.0013 | 0.0011 | 0.0012 | 0.0018 | 0.0037 | 0.0066 | 0.0102 | 0.0003 | 0.0111 | 0.0009 |
Endosome | 0.0216 | 0.0074 | 0.0064 | 0.0059 | 0.0597 | 0.0062 | 0.0191 | 0.0051 | 0.0142 | 0.0191 | 0.0549 | 0.0282 | 0.0441 | 0.0152 | 0.0195 | 0.0018 | 0.0577 | 0.0047 |
Golgi | 0.0067 | 0.0007 | 0.0035 | 0.002 | 0.0203 | 0.001 | 0.0034 | 0.0022 | 0.009 | 0.007 | 0.0549 | 0.011 | 0.0176 | 0.0012 | 0.0047 | 0.0007 | 0.0011 | 0.0004 |
Lipid Particles | 0.0227 | 0.0048 | 0.0073 | 0.0143 | 0.07 | 0.0049 | 0.0098 | 0.0032 | 0.0073 | 0.0164 | 0.054 | 0.0034 | 0.0553 | 0.0131 | 0.0097 | 0.0061 | 0.0106 | 0.0009 |
Mitochondria | 0.0141 | 0.0003 | 0.0015 | 0.0026 | 0.0246 | 0.0011 | 0.002 | 0.0009 | 0.0052 | 0.0019 | 0.0341 | 0.0027 | 0.0038 | 0.0004 | 0.0168 | 0.0005 | 0.0011 | 0.0001 |
None | 0.6277 | 0.8166 | 0.7421 | 0.6978 | 0.5861 | 0.7239 | 0.6616 | 0.7952 | 0.6945 | 0.6538 | 0.6122 | 0.7116 | 0.4334 | 0.6139 | 0.7076 | 0.7686 | 0.7061 | 0.6178 |
Nuclear Periphery | 0.0243 | 0.0017 | 0.0015 | 0.0008 | 0.0112 | 0.0039 | 0.0023 | 0.0015 | 0.0016 | 0.0026 | 0.002 | 0.0023 | 0.0064 | 0.0042 | 0.0046 | 0.0008 | 0.0057 | 0.0021 |
Nucleolus | 0.0024 | 0.0007 | 0.0014 | 0.0027 | 0.0016 | 0.0011 | 0.0017 | 0.0006 | 0.0021 | 0.0006 | 0.0005 | 0.0051 | 0.0037 | 0.0008 | 0.0005 | 0.0009 | 0.0028 | 0.0004 |
Nucleus | 0.0097 | 0.0036 | 0.0081 | 0.0015 | 0.0021 | 0.004 | 0.0063 | 0.0016 | 0.006 | 0.0014 | 0.0005 | 0.0184 | 0.0047 | 0.0022 | 0.0016 | 0.001 | 0.0036 | 0.0085 |
Peroxisomes | 0.015 | 0.0055 | 0.011 | 0.0284 | 0.0322 | 0.0044 | 0.0039 | 0.0013 | 0.0156 | 0.0061 | 0.0329 | 0.0031 | 0.0381 | 0.0022 | 0.0146 | 0.0069 | 0.0011 | 0.0027 |
Punctate Nuclear | 0.0817 | 0.0476 | 0.0876 | 0.0705 | 0.0264 | 0.1167 | 0.0758 | 0.0598 | 0.0886 | 0.0714 | 0.0042 | 0.0689 | 0.1063 | 0.108 | 0.0319 | 0.1328 | 0.0059 | 0.1254 |
Vacuole | 0.0023 | 0.0004 | 0.0006 | 0.0013 | 0.0031 | 0.0012 | 0.0013 | 0.0008 | 0.0007 | 0.0006 | 0.0016 | 0.0038 | 0.0029 | 0.0012 | 0.0029 | 0.0002 | 0.0066 | 0.0005 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0001 | 0.0002 | 0.0025 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.001 | 0.0011 | 0.0008 | 0.0003 | 0.0013 | 0.0001 | 0.001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.1923 | 7.2661 | 6.0657 | 7.7132 | 12.5146 | 7.1082 | 8.9539 | 5.5784 | 7.1179 | 10.5333 |
Translational Efficiency | 1.0558 | 0.8532 | 1.0488 | 0.8243 | 0.8062 | 2.2053 | 0.7629 | 0.9702 | 1.0015 | 1.0447 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |