Standard name
Description Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0.06 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.89 0.91 0.96 0.94 0.91 0.85 0.87 0.86 0.86 0.72 0.83 0.96 0.97 0.96 0.18 0.19 0.14 0.66 0.74 0.63 0.78 0.78 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.38 0.29 0.35 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.13 0 0 0 0.06 0.12 0.07 0.17 0.11 0.36 0.17 0 0 0 0 0.05 0.06 0.14 0.06 0.13 0.1 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0.05 0.05 0 0 0.05 0 0 0 0 0.52 0.53 0.51 0.05 0.05 0.11 0.05 0.06 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.11 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 2 1 1 2 7 5
Bud 0 4 0 1 1 3 5 9 7 8 5 4 1 0 0 3 3 5 3 0 1 0 10 10
Bud Neck 1 2 2 14 28 13 20 32 13 30 3 10 8 4 2 4 2 3 0 0 0 0 1 5
Bud Site 0 0 0 1 0 0 0 3 0 0 3 1 0 0 0 9 4 9 – – – – – –
Cell Periphery 0 1 1 1 2 4 3 2 2 2 1 0 2 1 0 0 0 0 1 0 1 1 4 7
Cytoplasm 161 112 78 282 451 471 549 556 411 411 247 307 362 514 565 61 34 45 131 95 65 188 383 462
Endoplasmic Reticulum 1 3 2 1 2 2 1 1 0 2 1 0 0 1 0 14 7 14 3 0 0 0 1 3
Endosome 0 0 0 1 0 1 2 0 0 2 1 0 1 1 8 124 52 112 8 2 2 2 4 6
Golgi 1 0 0 0 1 0 1 0 0 0 0 0 1 0 2 1 1 0 2 2 0 0 2 1
Mitochondria 25 17 0 1 4 29 74 45 79 55 123 64 0 0 0 10 9 20 27 8 13 24 28 29
Nucleus 0 0 1 0 2 3 10 9 7 6 4 3 1 0 0 13 10 14 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 26 16 25 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 4 1 10 0 0 0 0 0 1
SpindlePole 1 1 4 7 13 20 29 31 10 20 17 12 9 13 17 170 94 163 9 6 11 13 27 26
Vac/Vac Membrane 2 0 0 1 5 4 5 6 6 0 4 6 0 1 0 42 20 33 4 5 2 1 1 6
Unique Cell Count 180 126 86 295 481 517 643 636 476 479 342 369 377 530 589 330 178 318 200 129 104 243 489 588
Labelled Cell Count 192 142 89 310 511 552 699 694 535 536 410 407 385 535 595 482 254 454 200 129 104 243 489 588


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 3.0 4.6 4.3 4.7 3.9 3.5 4.0 3.5 3.4 3.3 3.5 4.8 5.0 5.1 5.9 6.1 5.9 4.4 4.6 4.9
Std Deviation (1e-4) 0.6 0.7 1.7 1.6 1.9 2.2 1.5 1.8 1.6 1.1 1.4 1.2 1.2 1.4 1.9 1.2 1.8 1.6 1.1 1.3 1.6
Intensity Change (Log2) – – – -0.07 0.04 -0.24 -0.39 -0.18 -0.39 -0.42 -0.48 -0.4 0.06 0.13 0.16 0.37 0.41 0.37 -0.06 0.0 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.5 3.2 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7794 0.4488 0.2998 -0.017 1.0126 0.5287 -1.3508 -0.5551 -0.4419 -1.1014 -0.7599 -0.2955 -1.0883 -1.598 -1.3778 -1.6709 -2.1948 -1.8974
Actin 0.0296 0.0023 0.0173 0.0009 0.0355 0.0021 0.0271 0.001 0.0128 0.032 0.0112 0.0074 0.0628 0.0012 0.0132 0.0028 0.0002 0.0014
Bud 0.0024 0.0003 0.0007 0.0014 0.0013 0.0006 0.0016 0.0009 0.0006 0.0002 0.008 0.0006 0.0006 0.0001 0.0005 0 0.0001 0
Bud Neck 0.002 0.0002 0.0007 0.0053 0.001 0.0071 0.0023 0.0003 0.0009 0.0087 0.0007 0.0035 0.003 0.0003 0.0003 0.0004 0.001 0.0006
Bud Periphery 0.0057 0.0003 0.0015 0.0033 0.0026 0.0033 0.0047 0.0021 0.0008 0.0005 0.0293 0.002 0.0007 0.0001 0.0005 0 0.0003 0
Bud Site 0.0097 0.0021 0.0027 0.0185 0.003 0.0015 0.0102 0.0042 0.0024 0.0015 0.0023 0.0005 0.0096 0.0012 0.0027 0 0.0003 0.0001
Cell Periphery 0.0004 0.0001 0.0002 0.0002 0.0003 0.0001 0.0003 0.0001 0.0001 0.0005 0.0004 0.0001 0.0004 0.0001 0.0002 0 0.0005 0
Cytoplasm 0.0327 0.0498 0.0384 0.0438 0.0126 0.0485 0.0528 0.0466 0.0331 0.0867 0.0302 0.0641 0.0312 0.1109 0.0748 0.0111 0.1284 0.1592
Cytoplasmic Foci 0.0802 0.0548 0.0658 0.0977 0.0987 0.0652 0.1116 0.0709 0.1027 0.0868 0.0637 0.0602 0.1699 0.1167 0.0818 0.0639 0.0544 0.0739
Eisosomes 0.0007 0.0002 0.0003 0.0001 0.0005 0.0001 0.0004 0.0001 0.0003 0.0008 0.0002 0.0001 0.001 0.0002 0.0002 0.001 0.0002 0.0002
Endoplasmic Reticulum 0.0062 0.0007 0.0012 0.0006 0.0044 0.0025 0.0016 0.0012 0.0013 0.0011 0.0012 0.0018 0.0037 0.0066 0.0102 0.0003 0.0111 0.0009
Endosome 0.0216 0.0074 0.0064 0.0059 0.0597 0.0062 0.0191 0.0051 0.0142 0.0191 0.0549 0.0282 0.0441 0.0152 0.0195 0.0018 0.0577 0.0047
Golgi 0.0067 0.0007 0.0035 0.002 0.0203 0.001 0.0034 0.0022 0.009 0.007 0.0549 0.011 0.0176 0.0012 0.0047 0.0007 0.0011 0.0004
Lipid Particles 0.0227 0.0048 0.0073 0.0143 0.07 0.0049 0.0098 0.0032 0.0073 0.0164 0.054 0.0034 0.0553 0.0131 0.0097 0.0061 0.0106 0.0009
Mitochondria 0.0141 0.0003 0.0015 0.0026 0.0246 0.0011 0.002 0.0009 0.0052 0.0019 0.0341 0.0027 0.0038 0.0004 0.0168 0.0005 0.0011 0.0001
None 0.6277 0.8166 0.7421 0.6978 0.5861 0.7239 0.6616 0.7952 0.6945 0.6538 0.6122 0.7116 0.4334 0.6139 0.7076 0.7686 0.7061 0.6178
Nuclear Periphery 0.0243 0.0017 0.0015 0.0008 0.0112 0.0039 0.0023 0.0015 0.0016 0.0026 0.002 0.0023 0.0064 0.0042 0.0046 0.0008 0.0057 0.0021
Nucleolus 0.0024 0.0007 0.0014 0.0027 0.0016 0.0011 0.0017 0.0006 0.0021 0.0006 0.0005 0.0051 0.0037 0.0008 0.0005 0.0009 0.0028 0.0004
Nucleus 0.0097 0.0036 0.0081 0.0015 0.0021 0.004 0.0063 0.0016 0.006 0.0014 0.0005 0.0184 0.0047 0.0022 0.0016 0.001 0.0036 0.0085
Peroxisomes 0.015 0.0055 0.011 0.0284 0.0322 0.0044 0.0039 0.0013 0.0156 0.0061 0.0329 0.0031 0.0381 0.0022 0.0146 0.0069 0.0011 0.0027
Punctate Nuclear 0.0817 0.0476 0.0876 0.0705 0.0264 0.1167 0.0758 0.0598 0.0886 0.0714 0.0042 0.0689 0.1063 0.108 0.0319 0.1328 0.0059 0.1254
Vacuole 0.0023 0.0004 0.0006 0.0013 0.0031 0.0012 0.0013 0.0008 0.0007 0.0006 0.0016 0.0038 0.0029 0.0012 0.0029 0.0002 0.0066 0.0005
Vacuole Periphery 0.0021 0.0001 0.0001 0.0002 0.0025 0.0005 0.0003 0.0002 0.0003 0.0004 0.001 0.0011 0.0008 0.0003 0.0013 0.0001 0.001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.1923 7.2661 6.0657 7.7132 12.5146 7.1082 8.9539 5.5784 7.1179 10.5333
Translational Efficiency 1.0558 0.8532 1.0488 0.8243 0.8062 2.2053 0.7629 0.9702 1.0015 1.0447

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘