Standard name
Description Alcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05
Bud 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.78 0.63 0.69 0.67 0.5 0.56 0.5 0.5 0.49 0.42 0.35 0.79 0.84 0.85 0.38 0.32 0.27 0.28 0.4 0.36 0.33 0.38 0.3
Endoplasmic Reticulum 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.2 0.25 0.2 0.05 0.07 0 0.07 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.16 0.28 0.32 0.4 0.45 0.62 0.57 0.61 0.57 0.67 0.75 0.75 0.14 0.09 0.05 0.17 0.06 0.14 0.43 0.34 0.42 0.4 0.36 0.34
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0.07 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0.16 0.2 0.2 0 0 0 0.05 0.05 0.07
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0.19 0.12 0.07 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 9 3 2 0 7 13 23
Bud 1 0 1 1 6 2 0 4 4 3 0 3 4 6 1 8 1 10 0 2 0 5 9 12
Bud Neck 0 0 1 0 0 0 2 1 2 0 0 0 0 1 1 2 2 0 2 2 0 2 6 20
Bud Site 1 0 0 0 1 0 0 2 1 0 0 0 0 0 0 0 1 2 – – – – – –
Cell Periphery 1 1 0 0 0 0 0 1 0 0 0 0 1 3 1 6 5 5 0 0 0 1 2 6
Cytoplasm 112 93 12 43 56 54 60 65 57 42 47 45 254 368 456 70 54 44 56 57 17 94 155 137
Endoplasmic Reticulum 0 1 2 0 1 0 0 2 0 0 0 1 3 1 1 12 7 11 7 0 0 3 2 7
Endosome 12 1 0 1 0 1 0 0 0 0 0 0 18 16 27 37 42 32 11 9 1 20 21 21
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 1 0 3 3 5 8 1 0 0 1 3 1
Mitochondria 25 33 6 25 38 68 62 79 66 58 85 95 45 38 29 32 10 22 87 49 20 116 149 158
Nucleus 1 1 0 0 3 1 0 1 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 1 1 1 0 1 0 1 0 0 0 0 0 2 0 0 0 2 0
Nucleolus 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 1 2 1
Peroxisomes 4 0 0 0 0 0 0 0 0 0 0 0 7 10 18 20 19 12 2 0 0 2 5 3
SpindlePole 7 0 0 0 2 3 4 11 7 2 5 4 5 5 4 30 34 33 3 4 1 15 20 31
Vac/Vac Membrane 8 3 0 2 1 1 2 3 4 3 0 1 16 10 15 16 32 20 14 7 1 9 11 26
Unique Cell Count 157 120 19 62 84 109 108 129 115 86 113 127 323 437 537 186 171 162 204 144 49 290 413 466
Labelled Cell Count 173 133 22 72 110 130 132 170 143 108 138 149 357 459 558 238 213 208 204 144 49 290 413 466


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.7 3.4 3.5 3.5 3.5 2.7 3.1 2.9 2.6 2.7 2.4 4.5 4.2 4.4 5.4 6.3 6.5 4.0 4.3 5.1
Std Deviation (1e-4) 2.8 1.0 0.8 1.4 1.3 1.5 1.2 1.4 1.3 1.8 1.1 1.1 1.5 1.1 1.7 1.9 2.1 2.0 1.5 2.1 1.9
Intensity Change (Log2) – – – 0.06 0.04 0.04 -0.34 -0.11 -0.22 -0.37 -0.3 -0.51 0.4 0.32 0.39 0.68 0.9 0.95 0.25 0.36 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7146 1.7178 1.296 1.6301 1.6273 1.961 2.2161 3.8062 2.3719 2.6172 2.3209 2.8229 1.8376 3.7141 2.5468 3.0914 3.4909 2.869
Actin 0.0724 0.0167 0.0119 0.0046 0.021 0.0207 0.0241 0.0408 0.0276 0.0189 0.0483 0.0174 0.0226 0.0194 0.0483 0.0094 0.0589 0.0237
Bud 0.005 0.0033 0.0022 0.0059 0.0202 0.0015 0.002 0.0138 0.0042 0.0068 0.0041 0.0017 0.0016 0.0052 0.0055 0.002 0.0031 0.0006
Bud Neck 0.006 0.0022 0.0072 0.001 0.0012 0.006 0.0088 0.0011 0.0018 0.0055 0.0026 0.004 0.0053 0.0025 0.0027 0.0055 0.0025 0.0021
Bud Periphery 0.0192 0.0091 0.0051 0.0085 0.0354 0.0047 0.0058 0.0141 0.0064 0.0184 0.0181 0.0066 0.0043 0.0192 0.0161 0.0063 0.0076 0.0016
Bud Site 0.0068 0.0408 0.014 0.0021 0.0086 0.0022 0.0287 0.0446 0.0119 0.0022 0.0035 0.0008 0.0134 0.0589 0.0138 0.0073 0.003 0.0007
Cell Periphery 0.004 0.007 0.0083 0.0008 0.0011 0.0011 0.0017 0.0027 0.0031 0.0005 0.0022 0.001 0.0096 0.0023 0.0056 0.0024 0.0011 0.0018
Cytoplasm 0.0595 0.1292 0.0821 0.1926 0.1627 0.0747 0.1301 0.2722 0.1179 0.2452 0.1332 0.1344 0.0693 0.0684 0.0822 0.0695 0.0665 0.0772
Cytoplasmic Foci 0.1109 0.0782 0.0579 0.0742 0.0761 0.106 0.0724 0.0926 0.0991 0.1215 0.1476 0.0798 0.1302 0.1167 0.0713 0.1246 0.0982 0.125
Eisosomes 0.0009 0.0008 0.0024 0.0003 0.0003 0.0006 0.0017 0.0007 0.0045 0.0008 0.0011 0.0005 0.0038 0.0013 0.0026 0.0014 0.0013 0.001
Endoplasmic Reticulum 0.0106 0.009 0.0141 0.0023 0.018 0.0064 0.008 0.001 0.0023 0.0072 0.0034 0.0024 0.0126 0.0013 0.0088 0.0018 0.0068 0.0023
Endosome 0.1325 0.1035 0.1159 0.0178 0.1718 0.0807 0.1072 0.0506 0.0584 0.0719 0.1351 0.103 0.082 0.0715 0.0949 0.0889 0.0768 0.0656
Golgi 0.0509 0.0547 0.0552 0.0156 0.0578 0.0629 0.047 0.0525 0.0554 0.0169 0.0883 0.0728 0.0304 0.0786 0.0587 0.0358 0.0947 0.094
Lipid Particles 0.0538 0.0094 0.022 0.0073 0.0148 0.04 0.0309 0.0125 0.0061 0.0033 0.0145 0.0204 0.04 0.0251 0.0182 0.0113 0.0214 0.0249
Mitochondria 0.2529 0.4184 0.4491 0.4915 0.2835 0.4096 0.2755 0.2764 0.3235 0.2599 0.2441 0.405 0.2652 0.2917 0.3672 0.3699 0.3866 0.3729
None 0.1285 0.0682 0.1024 0.1616 0.071 0.1111 0.1902 0.1 0.1895 0.1391 0.0924 0.0802 0.1789 0.1375 0.1463 0.1486 0.1332 0.1225
Nuclear Periphery 0.0044 0.001 0.0018 0.0006 0.0021 0.0016 0.0067 0.0003 0.0004 0.0067 0.0008 0.0006 0.0033 0.0005 0.0012 0.0006 0.0037 0.0004
Nucleolus 0.0007 0.0011 0.001 0.0002 0.0007 0.001 0.0014 0.0008 0.0005 0.0003 0.0005 0.0004 0.0041 0.0007 0.0005 0.001 0.0005 0.0003
Nucleus 0.0013 0.0015 0.0011 0.0012 0.0016 0.0114 0.0026 0.0011 0.0006 0.0019 0.0007 0.0006 0.0065 0.0008 0.0011 0.0008 0.0013 0.0005
Peroxisomes 0.0606 0.0301 0.0321 0.0047 0.0246 0.047 0.0315 0.0171 0.081 0.0639 0.0492 0.0567 0.0811 0.0925 0.0433 0.1011 0.0211 0.074
Punctate Nuclear 0.0094 0.0004 0.0006 0.0016 0.0011 0.0011 0.0043 0.0006 0.0005 0.0011 0.0007 0.0005 0.007 0.0006 0.0008 0.003 0.004 0.0013
Vacuole 0.0066 0.0119 0.009 0.0038 0.0207 0.0059 0.0134 0.0034 0.0039 0.0048 0.0063 0.0074 0.0247 0.0037 0.0075 0.0059 0.0042 0.0049
Vacuole Periphery 0.0031 0.0034 0.0045 0.0017 0.0057 0.0038 0.006 0.0011 0.0015 0.0033 0.0031 0.0035 0.0042 0.0015 0.0036 0.003 0.0035 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.2143 27.4709 26.7548 24.1141 21.1666 21.1153 28.4268 28.4131 25.6874 28.5909
Translational Efficiency 0.6482 1.1152 0.5815 0.6174 0.7058 1.4569 0.8031 0.4557 0.5259 0.4783

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘