Standard name
Description Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.99 0.95 0.9 0.81 0.47 0.43 0.54 0.42 0.39 0.44 0.95 0.96 0.96 0.91 0.95 0.9 0.95 0.94 0.96 0.88 0.88 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.24 0.26 0.27 0.27 0.3 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.07 0.05 0.13 0.15 0.15 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.09 0.09 0.38 0.33 0.25 0.32 0.35 0.27 0.08 0.06 0.06 0.11 0.06 0.12 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 2
Bud 0 1 0 0 0 0 1 6 2 2 1 2 1 0 1 1 0 0 0 0 0 1 7 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 2 0 1 1 0 5 1 1 1 0 1 0 0 0 0 0 0 0
Cytoplasm 315 256 152 188 334 170 186 120 112 133 109 167 291 383 381 199 220 148 314 253 147 186 310 340
Endoplasmic Reticulum 4 3 0 1 5 2 1 1 0 3 1 1 2 6 5 3 4 8 1 0 0 2 3 11
Endosome 0 0 1 0 1 0 2 0 0 1 5 0 0 1 0 1 0 1 2 1 0 0 1 5
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2 2
Mitochondria 2 2 2 3 10 24 93 71 57 86 85 133 0 0 0 2 3 1 3 0 1 2 2 3
Nucleus 4 2 0 1 3 15 20 37 32 47 34 44 5 3 3 5 4 4 3 3 0 5 5 3
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 1 0 0 0 0 2
Nucleolus 0 0 0 1 1 0 1 1 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 10 7 0 14 32 19 147 91 53 101 99 103 23 22 22 25 14 19 0 2 0 11 12 12
Unique Cell Count 327 267 154 197 371 209 392 278 209 315 279 382 305 400 395 218 231 164 331 270 154 213 354 403
Labelled Cell Count 336 272 155 208 386 231 453 327 259 374 337 456 323 416 413 236 246 183 331 270 154 213 354 403


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 10.8 6.7 6.3 6.8 6.8 6.4 6.6 6.8 6.7 6.9 6.7 9.1 9.2 10.1 11.3 11.5 11.7 10.1 10.7 11.2
Std Deviation (1e-4) 1.2 1.7 1.1 1.2 1.4 1.4 1.3 1.5 1.7 1.5 1.7 1.6 1.4 1.4 1.5 2.7 2.1 2.6 2.4 2.4 2.1
Intensity Change (Log2) – – – -0.08 0.04 0.04 -0.06 -0.01 0.03 -0.0 0.04 0.01 0.45 0.46 0.6 0.76 0.79 0.81 0.6 0.68 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.7 -3.5 -5.2 -11.1 -11.4 -9.5 -11.8 -12.1 -11.7 -1.8 -1.7 -1.4 -3.1 -1.8 -3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 6.2 6.4 6.6 6.8 7.3 8.1 0 0 0 0 0 0
Nucleus 0 0 3.4 2.9 4.7 5.1 5.1 4.5 4.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.4 3.8 3.8 8.9 8.0 6.8 7.9 8.4 7.2 3.5 3.0 3.0 4.4 3.1 4.4