Standard name
Human Ortholog
Description Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.99 0.95 0.9 0.81 0.47 0.43 0.54 0.42 0.39 0.44 0.95 0.96 0.96 0.91 0.95 0.9 0.95 0.94 0.96 0.88 0.88 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.24 0.26 0.27 0.27 0.3 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.07 0.05 0.13 0.15 0.15 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.09 0.09 0.38 0.33 0.25 0.32 0.35 0.27 0.08 0.06 0.06 0.11 0.06 0.12 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 2
Bud 0 1 0 0 0 0 1 6 2 2 1 2 1 0 1 1 0 0 0 0 0 1 7 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 2 0 1 1 0 5 1 1 1 0 1 0 0 0 0 0 0 0
Cytoplasm 315 256 152 188 334 170 186 120 112 133 109 167 291 383 381 199 220 148 314 253 147 186 310 340
Endoplasmic Reticulum 4 3 0 1 5 2 1 1 0 3 1 1 2 6 5 3 4 8 1 0 0 2 3 11
Endosome 0 0 1 0 1 0 2 0 0 1 5 0 0 1 0 1 0 1 2 1 0 0 1 5
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2 2
Mitochondria 2 2 2 3 10 24 93 71 57 86 85 133 0 0 0 2 3 1 3 0 1 2 2 3
Nucleus 4 2 0 1 3 15 20 37 32 47 34 44 5 3 3 5 4 4 3 3 0 5 5 3
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 1 0 0 0 0 2
Nucleolus 0 0 0 1 1 0 1 1 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 10 7 0 14 32 19 147 91 53 101 99 103 23 22 22 25 14 19 0 2 0 11 12 12
Unique Cell Count 327 267 154 197 371 209 392 278 209 315 279 382 305 400 395 218 231 164 331 270 154 213 354 403
Labelled Cell Count 336 272 155 208 386 231 453 327 259 374 337 456 323 416 413 236 246 183 331 270 154 213 354 403


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 10.8 6.7 6.3 6.8 6.8 6.4 6.6 6.8 6.7 6.9 6.7 9.1 9.2 10.1 11.3 11.5 11.7 10.1 10.7 11.2
Std Deviation (1e-4) 1.2 1.7 1.1 1.2 1.4 1.4 1.3 1.5 1.7 1.5 1.7 1.6 1.4 1.4 1.5 2.7 2.1 2.6 2.4 2.4 2.1
Intensity Change (Log2) -0.08 0.04 0.04 -0.06 -0.01 0.03 -0.0 0.04 0.01 0.45 0.46 0.6 0.76 0.79 0.81 0.6 0.68 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.7 -3.5 -5.2 -11.1 -11.4 -9.5 -11.8 -12.1 -11.7 -1.8 -1.7 -1.4 -3.1 -1.8 -3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 6.2 6.4 6.6 6.8 7.3 8.1 0 0 0 0 0 0
Nucleus 0 0 3.4 2.9 4.7 5.1 5.1 4.5 4.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.4 3.8 3.8 8.9 8.0 6.8 7.9 8.4 7.2 3.5 3.0 3.0 4.4 3.1 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0006 0.0023 0.0003 0.0002 0.0003 0.0003 0.023 0.0007 0.0013 0.0007 0.0009 0.0004 0.0016 0.0003 0.0001 0 0.0003 0.0243
Bud 0.0017 0.0052 0.0002 0.0001 0.0003 0.0002 0.001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0 0.0001 0.0001 0.0005
Bud Neck 0.0005 0.0006 0.0006 0.0011 0.0013 0.0016 0.0027 0.0003 0.0005 0.001 0.0006 0.0014 0.0005 0.0001 0.0002 0.0001 0.0004 0.0014
Bud Periphery 0.0004 0.0024 0.0001 0 0.0002 0.0001 0.0017 0 0.0001 0.0001 0 0 0.0002 0 0 0 0 0.0004
Bud Site 0.0006 0.0027 0.0004 0.0002 0.0002 0.0001 0.0014 0.0008 0.0015 0.0004 0.0001 0.0001 0.0045 0.0002 0.0002 0 0.0001 0.0008
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0002 0.0001 0 0 0 0
Cytoplasm 0.7909 0.7479 0.8072 0.6841 0.7893 0.747 0.8158 0.9211 0.8666 0.8795 0.8867 0.8659 0.6649 0.9381 0.9769 0.9858 0.7533 0.7076
Cytoplasmic Foci 0.0055 0.0027 0.0037 0.0023 0.0042 0.0066 0.0198 0.0036 0.0054 0.0047 0.0052 0.004 0.0401 0.0027 0.0018 0.0017 0.0069 0.0789
Eisosomes 0 0 0 0 0 0 0.0006 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0046 0.0028 0.0038 0.0015 0.0033 0.0029 0.0058 0.0028 0.0034 0.0076 0.0022 0.0021 0.005 0.0084 0.0009 0.0001 0.0167 0.0032
Endosome 0.0039 0.0018 0.0025 0.0014 0.0032 0.0048 0.0171 0.0015 0.0041 0.0056 0.0016 0.0022 0.0802 0.0028 0.0003 0.0002 0.1902 0.0707
Golgi 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0037 0.0001 0.0014 0.0002 0.0001 0.0006 0.0227 0.0002 0 0 0.0073 0.0702
Lipid Particles 0.0001 0 0 0 0 0 0.0021 0 0.0004 0.0001 0.0001 0.0017 0.0398 0.0003 0 0 0.0004 0.0056
Mitochondria 0.0003 0.0004 0.0003 0.0002 0.0035 0.0003 0.0017 0.0002 0.0036 0.0003 0.0001 0.0007 0.0021 0.0001 0 0 0.0022 0.0055
None 0.0184 0.0118 0.0164 0.0143 0.0056 0.008 0.0225 0.0074 0.0262 0.0097 0.003 0.0092 0.0816 0.0316 0.0024 0.0045 0.0057 0.0025
Nuclear Periphery 0.0103 0.0103 0.0079 0.0056 0.0089 0.0075 0.0083 0.0037 0.0044 0.0099 0.004 0.0036 0.0046 0.002 0.0012 0.0003 0.0049 0.0013
Nucleolus 0.0002 0.0002 0.0002 0.0002 0.0002 0.0001 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0015 0 0 0 0 0
Nucleus 0.1561 0.2039 0.152 0.2845 0.1724 0.2116 0.0589 0.0555 0.0762 0.0757 0.0928 0.1032 0.0109 0.0116 0.0152 0.0066 0.0033 0.0063
Peroxisomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0071 0.0002 0.001 0.0001 0.0001 0.0017 0.0059 0 0 0 0 0.0173
Punctate Nuclear 0.0037 0.0035 0.0031 0.0033 0.0052 0.0075 0.004 0.0014 0.0024 0.0015 0.0018 0.0022 0.0027 0.0006 0.0004 0.0003 0.0004 0.0024
Vacuole 0.0016 0.001 0.001 0.0007 0.0013 0.001 0.0018 0.0005 0.0008 0.0021 0.0003 0.0007 0.0284 0.0007 0.0002 0.0002 0.0048 0.0006
Vacuole Periphery 0.0003 0.0002 0.0002 0.0001 0.0005 0.0002 0.0005 0.0001 0.0001 0.0003 0.0001 0.0001 0.0023 0.0001 0 0 0.0031 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.8169 23.2773 14.394 23.3938 16.8804 35.7839 24.7615 22.5037 24.53 21.8038
Translational Efficiency 1.9086 1.7696 2.0287 1.8147 1.9148 1.1877 1.8693 2.2381 2.1689 1.8659

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sds22

Sds22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sds22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available