TheCellVision

SDS22

Standard name	SDS22 ORF YKL193C Aliases EGP1, type 1 protein phosphatase-activating protein SDS22
Human Ortholog	PPP1R7
Description	Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function

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Scale images

sds22-5
sds22-6

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	0.96	0.96	0.99	0.95	0.9	0.81	0.47	0.43	0.54	0.42	0.39	0.44	0.95	0.96	0.96	0.91	0.95	0.9	0.95	0.94	0.96	0.88	0.88	0.85
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	0.11	0.24	0.26	0.27	0.27	0.3	0.35	0	0	0	0	0	0	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0.07	0.05	0.13	0.15	0.15	0.12	0.12	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0.07	0.09	0.09	0.38	0.33	0.25	0.32	0.35	0.27	0.08	0.06	0.06	0.11	0.06	0.12	0	0	0	0.05	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	2	2
Bud	0	1	0	0	0	0	1	6	2	2	1	2	1	0	1	1	0	0	0	0	0	1	7	3
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Bud Site	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	1	2	0	1	1	0	5	1	1	1	0	1	0	0	0	0	0	0	0
Cytoplasm	315	256	152	188	334	170	186	120	112	133	109	167	291	383	381	199	220	148	314	253	147	186	310	340
Endoplasmic Reticulum	4	3	0	1	5	2	1	1	0	3	1	1	2	6	5	3	4	8	1	0	0	2	3	11
Endosome	0	0	1	0	1	0	2	0	0	1	5	0	0	1	0	1	0	1	2	1	0	0	1	5
Golgi	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	2	2
Mitochondria	2	2	2	3	10	24	93	71	57	86	85	133	0	0	0	2	3	1	3	0	1	2	2	3
Nucleus	4	2	0	1	3	15	20	37	32	47	34	44	5	3	3	5	4	4	3	3	0	5	5	3
Nuclear Periphery	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	0	0	0	1	0	0	0	0	2
Nucleolus	0	0	0	1	1	0	1	1	0	0	1	1	0	0	0	0	0	1	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Vac/Vac Membrane	10	7	0	14	32	19	147	91	53	101	99	103	23	22	22	25	14	19	0	2	0	11	12	12
Unique Cell Count	327	267	154	197	371	209	392	278	209	315	279	382	305	400	395	218	231	164	331	270	154	213	354	403
Labelled Cell Count	336	272	155	208	386	231	453	327	259	374	337	456	323	416	413	236	246	183	331	270	154	213	354	403

Yeast GFP Assignment

Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	9.2	10.8	6.7	6.3	6.8	6.8	6.4	6.6	6.8	6.7	6.9	6.7	9.1	9.2	10.1	11.3	11.5	11.7	10.1	10.7	11.2
Std Deviation (1e^-4)	1.2	1.7	1.1	1.2	1.4	1.4	1.3	1.5	1.7	1.5	1.7	1.6	1.4	1.4	1.5	2.7	2.1	2.6	2.4	2.4	2.1
Intensity Change (Log₂)	–	–	–	-0.08	0.04	0.04	-0.06	-0.01	0.03	-0.0	0.04	0.01	0.45	0.46	0.6	0.76	0.79	0.81	0.6	0.68	0.75

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	-1.7	-3.5	-5.2	-11.1	-11.4	-9.5	-11.8	-12.1	-11.7	-1.8	-1.7	-1.4	-3.1	-1.8	-3.3
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	3.7	6.2	6.4	6.6	6.8	7.3	8.1	0	0	0	0	0	0
Nucleus	0	0	3.4	2.9	4.7	5.1	5.1	4.5	4.4	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vacuole	3.4	3.8	3.8	8.9	8.0	6.8	7.9	8.4	7.2	3.5	3.0	3.0	4.4	3.1	4.4

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Actin	0.0006	0.0023	0.0003	0.0002	0.0003	0.0003	0.023	0.0007	0.0013	0.0007	0.0009	0.0004	0.0016	0.0003	0.0001	0	0.0003	0.0243
Bud	0.0017	0.0052	0.0002	0.0001	0.0003	0.0002	0.001	0.0001	0.0002	0.0002	0.0001	0.0001	0.0003	0.0001	0	0.0001	0.0001	0.0005
Bud Neck	0.0005	0.0006	0.0006	0.0011	0.0013	0.0016	0.0027	0.0003	0.0005	0.001	0.0006	0.0014	0.0005	0.0001	0.0002	0.0001	0.0004	0.0014
Bud Periphery	0.0004	0.0024	0.0001	0	0.0002	0.0001	0.0017	0	0.0001	0.0001	0	0	0.0002	0	0	0	0	0.0004
Bud Site	0.0006	0.0027	0.0004	0.0002	0.0002	0.0001	0.0014	0.0008	0.0015	0.0004	0.0001	0.0001	0.0045	0.0002	0.0002	0	0.0001	0.0008
Cell Periphery	0.0001	0.0001	0	0	0	0	0.0001	0	0.0001	0	0	0	0.0002	0.0001	0	0	0	0
Cytoplasm	0.7909	0.7479	0.8072	0.6841	0.7893	0.747	0.8158	0.9211	0.8666	0.8795	0.8867	0.8659	0.6649	0.9381	0.9769	0.9858	0.7533	0.7076
Cytoplasmic Foci	0.0055	0.0027	0.0037	0.0023	0.0042	0.0066	0.0198	0.0036	0.0054	0.0047	0.0052	0.004	0.0401	0.0027	0.0018	0.0017	0.0069	0.0789
Eisosomes	0	0	0	0	0	0	0.0006	0	0	0	0	0	0	0	0	0	0	0
Endoplasmic Reticulum	0.0046	0.0028	0.0038	0.0015	0.0033	0.0029	0.0058	0.0028	0.0034	0.0076	0.0022	0.0021	0.005	0.0084	0.0009	0.0001	0.0167	0.0032
Endosome	0.0039	0.0018	0.0025	0.0014	0.0032	0.0048	0.0171	0.0015	0.0041	0.0056	0.0016	0.0022	0.0802	0.0028	0.0003	0.0002	0.1902	0.0707
Golgi	0.0001	0.0001	0.0001	0	0.0001	0.0001	0.0037	0.0001	0.0014	0.0002	0.0001	0.0006	0.0227	0.0002	0	0	0.0073	0.0702
Lipid Particles	0.0001	0	0	0	0	0	0.0021	0	0.0004	0.0001	0.0001	0.0017	0.0398	0.0003	0	0	0.0004	0.0056
Mitochondria	0.0003	0.0004	0.0003	0.0002	0.0035	0.0003	0.0017	0.0002	0.0036	0.0003	0.0001	0.0007	0.0021	0.0001	0	0	0.0022	0.0055
None	0.0184	0.0118	0.0164	0.0143	0.0056	0.008	0.0225	0.0074	0.0262	0.0097	0.003	0.0092	0.0816	0.0316	0.0024	0.0045	0.0057	0.0025
Nuclear Periphery	0.0103	0.0103	0.0079	0.0056	0.0089	0.0075	0.0083	0.0037	0.0044	0.0099	0.004	0.0036	0.0046	0.002	0.0012	0.0003	0.0049	0.0013
Nucleolus	0.0002	0.0002	0.0002	0.0002	0.0002	0.0001	0.0002	0	0.0002	0.0001	0.0001	0.0001	0.0015	0	0	0	0	0
Nucleus	0.1561	0.2039	0.152	0.2845	0.1724	0.2116	0.0589	0.0555	0.0762	0.0757	0.0928	0.1032	0.0109	0.0116	0.0152	0.0066	0.0033	0.0063
Peroxisomes	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001	0.0071	0.0002	0.001	0.0001	0.0001	0.0017	0.0059	0	0	0	0	0.0173
Punctate Nuclear	0.0037	0.0035	0.0031	0.0033	0.0052	0.0075	0.004	0.0014	0.0024	0.0015	0.0018	0.0022	0.0027	0.0006	0.0004	0.0003	0.0004	0.0024
Vacuole	0.0016	0.001	0.001	0.0007	0.0013	0.001	0.0018	0.0005	0.0008	0.0021	0.0003	0.0007	0.0284	0.0007	0.0002	0.0002	0.0048	0.0006
Vacuole Periphery	0.0003	0.0002	0.0002	0.0001	0.0005	0.0002	0.0005	0.0001	0.0001	0.0003	0.0001	0.0001	0.0023	0.0001	0	0	0.0031	0.0004

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	16.8169	23.2773	14.394	23.3938	16.8804	35.7839	24.7615	22.5037	24.53	21.8038
Translational Efficiency	1.9086	1.7696	2.0287	1.8147	1.9148	1.1877	1.8693	2.2381	2.1689	1.8659

Hit Data

Dataset	Hit
Protein Concentration	–
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
2176	1529	214	256	1755	1518	1046	1874	3931	3047	1260	2130

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	841.71	955.32	1509.72	1536.18	912.08	875.02	1145.52	1327.00	873.13	915.31	1207.38	1352.14
Standard Deviation	121.56	145.08	216.06	266.33	126.16	125.97	216.44	194.13	128.49	141.70	255.97	215.20
Intensity Change Log ₂	—	0.182661	0.842886	0.867952	—	-0.059844	0.328770	0.540936	—	0.061636	0.598366	0.707142

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000397	0.002019	0.006172	0.025685	0.000863	0.002217	0.010550	0.014583	0.000605	0.002118	0.009807	0.015917
Bud Neck	0.013257	0.004885	0.005194	0.007351	0.006731	0.003475	0.002241	0.003971	0.010343	0.004182	0.002743	0.004377
Bud Site	0.013634	0.026801	0.024968	0.104556*	0.019410	0.021230	0.033420	0.062699	0.016213	0.024025	0.031984	0.067729
Cell Periphery	0.000091	0.000276	0.000196	0.000202	0.000104	0.000093	0.000114	0.000148	0.000096	0.000184	0.000128	0.000155
Cytoplasm	0.426393*	0.380011*	0.069688	0.164769*	0.450103*	0.176685*	0.089732	0.138460*	0.436978*	0.278715*	0.086327	0.141622*
Cytoplasmic Foci	0.279572*	0.236548*	0.206754*	0.245387*	0.224744*	0.276573*	0.230500*	0.276054*	0.255094*	0.256488*	0.226467*	0.272368*
Eisosomes	0.000089	0.000043	0.000028	0.000052	0.000045	0.000065	0.000034	0.000050	0.000069	0.000054	0.000033	0.000050
Endoplasmic Reticulum	0.001180	0.001599	0.003495	0.000797	0.001971	0.001551	0.001052	0.000642	0.001533	0.001575	0.001467	0.000660
Endosome	0.047517	0.056419	0.161586	0.073228	0.038248	0.113709	0.131458	0.137408	0.043379	0.084961	0.136575	0.129694
Golgi	0.051592	0.134128*	0.149736*	0.047643	0.094931	0.283071*	0.309623*	0.189820*	0.070941	0.208331*	0.282467*	0.172732*
Lipid Particles	0.007920	0.004072	0.005491	0.011962	0.003101	0.004921	0.007474	0.006086	0.005769	0.004495	0.007137	0.006792
Mitochondria	0.020944	0.009543	0.000402	0.000130	0.014868	0.022558	0.002256	0.003782	0.018232	0.016027	0.001941	0.003343
Mitotic Spindle	0.008594	0.031574	0.173492*	0.102976*	0.029047	0.022243	0.094854	0.071437	0.017725	0.026926	0.108210*	0.075227
None	0.003012	0.002881	0.000886	0.003272	0.002543	0.000644	0.000566	0.000246	0.002803	0.001767	0.000621	0.000610
Nuclear Periphery	0.000182	0.000156	0.001679	0.000274	0.000166	0.000117	0.000257	0.000110	0.000175	0.000137	0.000498	0.000130
Nuclear Periphery Foci	0.001054	0.002384	0.011482	0.001800	0.001542	0.001136	0.006418	0.000901	0.001272	0.001762	0.007278	0.001009
Nucleolus	0.001286	0.000531	0.000156	0.000101	0.000497	0.000319	0.000133	0.000081	0.000934	0.000426	0.000137	0.000083
Nucleus	0.059521	0.051709	0.082856	0.063787	0.069526	0.013906	0.020715	0.004973	0.063987	0.032876	0.031269	0.012042
Peroxisomes	0.008424	0.009771	0.007121	0.051292	0.006453	0.010777	0.007184	0.021776	0.007544	0.010272	0.007173	0.025323
Vacuole	0.050623	0.040388	0.081544	0.093951	0.030495	0.035732	0.043588	0.058856	0.041637	0.038068	0.050035	0.063074
Vacuole Periphery	0.004718	0.004259	0.007078	0.000786	0.004613	0.008979	0.007832	0.007919	0.004671	0.006611	0.007704	0.007061

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-5.42	-2.20	-3.24	-2.55	-1.28	-3.25	-6.31	-8.80	-7.22	-0.57	-5.89	-6.79	-9.55	-7.93	-1.02
Bud Neck	7.73	4.50	1.81	-4.58	-1.95	4.00	4.76	3.02	-2.09	-3.66	8.87	9.70	7.77	-2.23	-3.43
Bud Site	-5.85	-1.70	-5.26	-3.41	-4.30	-0.88	-5.49	-12.60	-11.22	-5.47	-5.10	-7.10	-15.66	-11.35	-6.89
Cell Periphery	-2.41	-7.41	-6.99	-2.02	-0.73	1.41	-2.67	-3.05	-4.03	-1.07	-2.26	-7.76	-7.01	0.15	-0.18
Cytoplasm	3.21	20.02	13.09	9.99	-4.23	22.61	26.48	20.50	-4.27	-8.81	17.68	33.63	25.84	6.88	-9.74
Cytoplasmic Foci	6.31	12.61	18.20	13.51	2.66	-7.00	2.93	2.16	9.99	-1.02	0.37	10.54	10.62	10.40	-0.86
Eisosomes	7.30	9.25	6.15	-1.02	-3.62	-4.19	3.76	0.49	4.41	-2.94	3.61	9.88	6.24	2.97	-3.64
Endoplasmic Reticulum	-1.81	-2.54	-1.21	0.77	2.29	0.69	1.61	3.15	1.79	4.79	-0.17	-0.73	3.43	3.10	4.56
Endosome	-1.99	-4.48	6.19	7.28	6.81	-17.92	-17.62	-17.53	2.38	2.77	-14.63	-17.30	-15.53	-2.34	4.22
Golgi	-13.01	-2.73	9.70	18.35	6.02	-22.25*	-17.87	-6.36	16.83	13.33	-25.44*	-20.61	-10.18	13.02	13.08
Lipid Particles	5.00	2.55	0.23	-3.32	-1.75	-2.42	-4.68	-6.03	-1.19	1.53	2.17	-1.68	-0.83	-2.98	1.16
Mitochondria	6.15	12.99	13.16	8.23	2.82	-3.39	8.64	8.06	10.99	-1.35	1.58	14.70	13.80	12.20	-1.49
Mitotic Spindle	-7.67	-5.95	-4.28	-1.81	3.05	1.94	-9.34	-7.91	-9.90	3.27	-4.05	-12.84	-11.98	-9.40	4.16
None	0.26	1.69	-2.94	-3.04	-3.77	8.28	8.57	7.66	-1.78	-1.21	3.65	7.82	5.03	0.89	-2.89
Nuclear Periphery	0.85	-8.89	-9.09	-9.21	2.60	2.29	-6.98	-8.30	-8.46	1.06	2.23	-10.29	-11.40	-12.20	3.72
Nuclear Periphery Foci	-3.05	-5.13	-7.09	-1.88	3.70	1.41	-5.12	0.50	-1.07	5.21	-2.06	-6.91	-3.08	0.43	6.34
Nucleolus	2.27	3.10	3.11	1.70	0.09	0.70	4.63	5.18	1.55	0.54	2.41	4.19	4.34	3.08	0.81
Nucleus	1.50	-9.42	-10.66	-10.93	0.65	16.99	4.08	10.19	-7.89	3.17	12.66	-3.28	1.91	-6.94	4.11
Peroxisomes	-1.24	3.54	-1.75	-1.34	-2.85	-4.30	-0.14	-8.20	-4.00	-7.58	-3.74	1.90	-7.82	-4.86	-8.26
Vacuole	1.22	-7.10	-12.50	-12.67	-5.89	-2.71	-7.89	-19.91	-16.97	-10.33	-0.32	-8.35	-20.99	-20.22	-10.70
Vacuole Periphery	1.19	0.50	12.46	10.07	3.83	-5.76	-2.84	-1.45	3.26	1.13	-4.09	-2.66	-0.68	1.89	1.50