Standard name
Description Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.1 0.09 0.09 0.13 0.08 0.13 0.08 0.11 0.08 0.08 0.05 0.13 0.15 0.26 0.2 0.13 0.16 0.05 0.06 0 0.15 0.14 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.17 0 0.07 0 0.11 0.13 0.15 0.59 0.33 0.4 0.52 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Nucleus 0.8 0.74 0.86 0.82 0.78 0.82 0.73 0.75 0.61 0.71 0.69 0.63 0.75 0.82 0.68 0.53 0.6 0.7 0.77 0.73 0.79 0.62 0.56 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.15 0.07 0.05 0.08 0.07 0.06 0.08 0.05 0.07 0.09 0.1 0.16 0.08 0.09 0.3 0.35 0.22 0.1 0.14 0.11 0.15 0.17 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 0 0 0 0 1 1 3 2 4 5 0 0 0 0 0 0 10 7 2 0 3 7
Bud Neck 0 0 0 0 0 0 0 4 3 1 2 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 2 2 2 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 29 38 17 17 30 21 44 35 41 29 20 15 19 28 60 24 20 21 14 26 6 14 32 26
Endoplasmic Reticulum 1 1 1 0 1 0 0 1 0 0 0 0 0 3 1 8 7 5 0 0 0 0 1 1
Endosome 0 1 2 2 6 1 2 4 0 0 0 1 3 0 1 2 3 0 2 2 1 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0
Mitochondria 2 66 0 12 2 30 44 64 226 119 99 143 3 0 2 8 7 1 0 5 1 0 1 4
Nucleus 242 287 158 150 176 220 241 314 232 255 169 173 107 159 156 63 90 92 251 328 164 58 132 130
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Nucleolus 0 1 0 0 2 0 2 1 1 0 0 4 0 0 0 0 0 0 0 0 0 0 3 4
Peroxisomes 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 1 0 1 4 1 6 0 3 1 0 0 1 0 0 8 9 3 2 5 9
Vac/Vac Membrane 59 59 12 10 17 18 21 32 21 25 21 27 23 15 21 35 53 29 31 62 23 14 40 58
Unique Cell Count 303 387 184 182 225 268 330 417 382 361 246 276 142 193 229 118 151 132 326 453 208 94 236 256
Labelled Cell Count 333 453 191 193 235 290 357 460 530 440 317 372 156 205 241 141 180 148 326 453 208 94 236 256


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.4 6.2 5.7 6.2 5.1 4.9 5.1 4.0 4.4 4.4 4.2 5.8 5.9 5.4 5.3 6.4 6.8 6.6 6.5 6.3
Std Deviation (1e-4) 0.8 0.8 1.2 1.2 1.3 1.2 1.3 1.2 0.8 0.7 0.9 1.0 1.1 0.8 1.1 1.4 1.1 1.5 1.7 1.7 1.6
Intensity Change (Log2) -0.13 0.0 -0.28 -0.34 -0.27 -0.64 -0.5 -0.5 -0.55 -0.09 -0.08 -0.19 -0.23 0.04 0.13 0.08 0.07 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 5.4 6.6 4.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fzf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available