Standard name
Human Ortholog
Description Essential nuclear envelope integral membrane protein; interacts and functions with Apq12p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA nuclear export, and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism; homologous to Brl1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – – – 0 – – – – – – – 0.06 0 0.06 0 0 0.07 0 0 0.09
Bud 0 0 – – – – – 0.05 – – – – – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – – – – 0 – – – – – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – – – – 0.11 – – – – – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – – – – – 0 – – – – – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.16 – – – – – 0.16 – – – – – – – 0.34 0.41 0.36 0 0 0 0.07 0.05 0.1
Endoplasmic Reticulum 0 0 – – – – – 0 – – – – – – – 0 0 0 0 0 0.09 0.08 0.08 0.18
Endosome 0 0.11 – – – – – 0 – – – – – – – 0.29 0.25 0.24 0.11 0.2 0.28 0.25 0.35 0.14
Golgi 0 0 – – – – – 0 – – – – – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.78 0.33 – – – – – 0.74 – – – – – – – 0.15 0.13 0.13 0.11 0.12 0.18 0.18 0.22 0.15
Nucleus 0 0 – – – – – 0 – – – – – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.22 0.2 – – – – – 0 – – – – – – – 0 0.05 0.05 0.49 0.38 0.17 0.16 0.11 0.16
Nucleolus 0 0 – – – – – 0.05 – – – – – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – – – – 0 – – – – – – – 0 0 0 0 0 0 0.07 0 0
SpindlePole 0 0 – – – – – 0.26 – – – – – – – 0.1 0.07 0.05 0 0 0.11 0.12 0 0
Vac/Vac Membrane 0.22 0.35 – – – – – 0.05 – – – – – – – 0.2 0.16 0.24 0.06 0.06 0.06 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 – – – – – 0 – – – – – – – 8 5 7 0 9 1 0 1 3
Bud 0 1 – – – – – 1 – – – – – – – 4 4 2 0 4 0 0 0 0
Bud Neck 0 0 – – – – – 0 – – – – – – – 2 3 1 0 5 0 0 0 0
Bud Site 0 0 – – – – – 2 – – – – – – – 1 1 0 – – – – – –
Cell Periphery 0 1 – – – – – 0 – – – – – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 3 34 – – – – – 3 – – – – – – – 47 50 43 1 11 0 1 1 3
Endoplasmic Reticulum 0 8 – – – – – 0 – – – – – – – 6 2 4 0 9 1 1 2 5
Endosome 0 24 – – – – – 0 – – – – – – – 41 30 29 6 61 5 4 11 4
Golgi 0 0 – – – – – 0 – – – – – – – 0 0 1 2 1 0 0 0 0
Mitochondria 14 69 – – – – – 14 – – – – – – – 21 16 16 6 36 3 3 7 5
Nucleus 0 5 – – – – – 0 – – – – – – – 1 2 1 0 2 0 0 0 0
Nuclear Periphery 4 41 – – – – – 0 – – – – – – – 1 6 6 27 116 3 2 3 5
Nucleolus 0 1 – – – – – 1 – – – – – – – 1 1 2 1 3 0 0 0 0
Peroxisomes 0 3 – – – – – 0 – – – – – – – 1 0 0 2 4 0 1 1 1
SpindlePole 0 2 – – – – – 5 – – – – – – – 14 9 6 2 13 2 2 1 1
Vac/Vac Membrane 4 74 – – – – – 1 – – – – – – – 28 19 28 3 16 1 0 0 2
Unique Cell Count 18 209 19 140 121 119 56 304 20 17 33 34
Labelled Cell Count 25 264 27 176 148 146 56 304 20 17 33 34


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 5.0 – – – – – 4.1 – – – – – – – 5.7 5.9 5.9 – – –
Std Deviation (1e-4) 0.6 0.9 – – – – – 1.8 – – – – – – – 1.0 1.1 1.3 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1725 0.0197 -0.239 -0.0747 0.2194 -0.2409 1.0316 0.6765 0.8439 0.5529 1.1523 0.5049 3.7709 3.4721 3.2785 3.6014 2.876 3.2885
Actin 0.0483 0.0111 0.0082 0.0534 0.0297 0.0277 0.0566 0.0044 0.0138 0.0265 0.0457 0.0156 0.1047 0.0102 0.0171 0.0254 0.0052 0.0266
Bud 0.0013 0.0008 0.0001 0.0007 0.0006 0.0002 0.0006 0.0003 0.0003 0.0003 0.0005 0.0001 0.0008 0.0001 0.0002 0.0003 0 0.0002
Bud Neck 0.0015 0.0009 0.0022 0.0089 0.0009 0.0007 0.0031 0.0008 0.0004 0.0078 0.0113 0.0008 0.0029 0.0006 0.0016 0.0192 0.0004 0.0015
Bud Periphery 0.002 0.0015 0.0003 0.0018 0.0007 0.0003 0.0007 0.0007 0.0007 0.0008 0.0011 0.0006 0.0008 0.0002 0.0002 0.0006 0.0001 0.0003
Bud Site 0.0073 0.0097 0.0004 0.0047 0.0023 0.0002 0.0082 0.003 0.001 0.0014 0.0015 0.0001 0.0228 0.0009 0.0013 0.0021 0.0001 0.0005
Cell Periphery 0.0006 0.0005 0.0002 0.0005 0.0002 0.0001 0.0004 0.0004 0.0002 0.0003 0.0003 0.0002 0.0005 0.0003 0.0009 0.0008 0.0001 0.0002
Cytoplasm 0.0285 0.0396 0.0209 0.0034 0.0655 0.0117 0.0559 0.0452 0.0303 0.0363 0.0383 0.0361 0.0556 0.0301 0.1757 0.0326 0.037 0.05
Cytoplasmic Foci 0.0395 0.0405 0.0428 0.0944 0.0434 0.0111 0.0281 0.0292 0.024 0.0316 0.0503 0.0129 0.0601 0.0162 0.0576 0.0795 0.0526 0.0212
Eisosomes 0.0019 0.0011 0.0003 0.0005 0.0003 0.0003 0.0009 0.0003 0.0007 0.0005 0.0005 0.0004 0.002 0.0009 0.0002 0.002 0.0001 0.0004
Endoplasmic Reticulum 0.0318 0.0349 0.0428 0.0135 0.0724 0.0199 0.0345 0.0583 0.0705 0.0504 0.0197 0.0425 0.0569 0.025 0.3068 0.0481 0.0408 0.0589
Endosome 0.1204 0.0312 0.0389 0.0307 0.0636 0.0356 0.089 0.0538 0.0588 0.0339 0.0609 0.0376 0.0345 0.0389 0.0295 0.0509 0.0431 0.0391
Golgi 0.0252 0.0074 0.0083 0.0129 0.0569 0.0204 0.0119 0.0073 0.0098 0.0125 0.0077 0.0044 0.012 0.0122 0.0052 0.0103 0.0063 0.0328
Lipid Particles 0.1671 0.0487 0.1686 0.2265 0.2269 0.1 0.0832 0.102 0.1361 0.1042 0.0928 0.0448 0.0378 0.1887 0.0171 0.1932 0.1329 0.131
Mitochondria 0.0212 0.0281 0.0071 0.025 0.0137 0.0064 0.004 0.0239 0.0101 0.0092 0.0067 0.0059 0.0094 0.0035 0.002 0.0022 0.0012 0.0089
None 0.053 0.1373 0.0695 0.0044 0.0167 0.0165 0.1293 0.0867 0.0703 0.0606 0.1181 0.0311 0.2111 0.1398 0.1059 0.1766 0.0813 0.0967
Nuclear Periphery 0.3359 0.4825 0.4654 0.3837 0.3199 0.6704 0.3127 0.4049 0.4616 0.3422 0.318 0.675 0.1914 0.3195 0.1053 0.2114 0.4506 0.4305
Nucleolus 0.0053 0.003 0.0033 0.0213 0.002 0.0014 0.0062 0.0228 0.0084 0.0093 0.0044 0.0023 0.0301 0.0054 0.002 0.0057 0.001 0.0037
Nucleus 0.0261 0.0229 0.0325 0.0139 0.0304 0.0253 0.0391 0.0546 0.0392 0.0682 0.0599 0.0338 0.0452 0.0202 0.0573 0.0163 0.0145 0.0485
Peroxisomes 0.0113 0.0081 0.0144 0.0226 0.0188 0.0026 0.0112 0.0063 0.0034 0.0271 0.0134 0.0016 0.0277 0.005 0.0171 0.0115 0.0043 0.0024
Punctate Nuclear 0.0496 0.0655 0.0506 0.0554 0.0172 0.0337 0.1063 0.0687 0.0378 0.1563 0.1305 0.0337 0.0868 0.1728 0.0874 0.1024 0.1182 0.0363
Vacuole 0.0089 0.0062 0.0042 0.0078 0.005 0.0029 0.0098 0.0101 0.0065 0.009 0.007 0.0036 0.0032 0.0025 0.007 0.0032 0.0022 0.0038
Vacuole Periphery 0.0135 0.0186 0.0191 0.0141 0.0127 0.0126 0.0084 0.0162 0.0162 0.0118 0.0113 0.0168 0.0037 0.007 0.0028 0.0058 0.0079 0.0064

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1455 1.6606 6.1611 12.7669 8.4295 9.2828 10.4937 17.5647 9.4644 14.715
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
19 111 465 5 515 91 3 9 534 202 468 14

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 304.80 729.67 775.64 4518.20 633.65 807.43 1020.65 367.70 621.95 764.70 777.21 1850.02
Standard Deviation 4.17 93.31 117.45 2393.49 82.30 88.65 28.51 3.18 101.21 99.10 118.71 2449.71
Intensity Change Log 2 — 1.259381 1.347524 3.889813 — 0.349651 0.687730 -0.785157 — 0.711859 0.936668 2.380273

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000032 0.000203 0.000756 0.000122 0.000074 0.000117 0.000278 0.000036 0.000072 0.000164 0.000753 0.000066
Bud Neck 0.000010 0.004653 0.003302 0.000139 0.001947 0.001307 0.002087 0.000004 0.001878 0.003145 0.003294 0.000052
Bud Site 0.000019 0.002876 0.002325 0.002403 0.000695 0.000653 0.001964 0.000014 0.000671 0.001875 0.002323 0.000867
Cell Periphery 0.000065 0.000217 0.000162 0.000385 0.000153 0.000146 0.000150 0.000617 0.000150 0.000185 0.000162 0.000534
Cytoplasm 0.000035 0.281947* 0.097513 0.707148* 0.163851* 0.195292* 0.036748 0.000048 0.158023* 0.242909* 0.097123 0.252584*
Cytoplasmic Foci 0.000015 0.154299 0.013715 0.002060 0.086607 0.099866 0.001915 0.000008 0.083526 0.129777 0.013639 0.000741
Eisosomes 0.000061 0.000113 0.000051 0.000040 0.000105 0.000093 0.000077 0.000469 0.000103 0.000104 0.000051 0.000316
Endoplasmic Reticulum 0.000001 0.015341 0.027816 0.000002 0.015183 0.028554 0.012682 0.000001 0.014643 0.021293 0.027719 0.000001
Endosome 0.000001 0.014383 0.007016 0.000002 0.008175 0.023581 0.003610 0.000000 0.007884 0.018527 0.006994 0.000001
Golgi 0.000006 0.000645 0.000288 0.000129 0.002067 0.001729 0.000045 0.000003 0.001993 0.001134 0.000286 0.000048
Lipid Particles 0.000028 0.069374 0.018854 0.000035 0.311466* 0.227626* 0.001955 0.000037 0.300385* 0.140666* 0.018746 0.000036
Mitochondria 0.000011 0.003159 0.003328 0.000022 0.005961 0.003011 0.000293 0.000016 0.005750 0.003092 0.003309 0.000018
Mitotic Spindle 0.000335 0.014551 0.053561 0.000026 0.004952 0.012016 0.000336 0.000318 0.004788 0.013409 0.053220 0.000214
None 0.999231* 0.003369 0.006060 0.267909* 0.009194 0.002365 0.000897 0.997797* 0.044420 0.002917 0.006027 0.737123*
Nuclear Periphery 0.000000 0.027732 0.115654* 0.000008 0.087054 0.072153 0.003044 0.000000 0.083957 0.047743 0.114932* 0.000003
Nuclear Periphery Foci 0.000026 0.131747 0.428398* 0.001697 0.194490* 0.196653* 0.005902 0.000001 0.187571 0.160986 0.425690* 0.000607
Nucleolus 0.000031 0.008821 0.025181 0.000066 0.009421 0.004194 0.000270 0.000001 0.009087 0.006737 0.025021 0.000024
Nucleus 0.000013 0.231061* 0.173309* 0.016240 0.085160 0.109790 0.742802* 0.000005 0.082131 0.176429* 0.176959* 0.005803
Peroxisomes 0.000079 0.002812 0.001178 0.000037 0.007882 0.003518 0.000078 0.000624 0.007605 0.003130 0.001171 0.000414
Vacuole 0.000001 0.030872 0.020854 0.001510 0.005078 0.016229 0.184737 0.000000 0.004898 0.024276 0.021905 0.000539
Vacuole Periphery 0.000000 0.001825 0.000679 0.000020 0.000484 0.001107 0.000132 0.000000 0.000467 0.001501 0.000675 0.000007

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Bud Neck NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Bud Site NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cell Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cytoplasm NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cytoplasmic Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Eisosomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Endoplasmic Reticulum NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Endosome NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Golgi NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Lipid Particles NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Mitochondria NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Mitotic Spindle NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
None NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nuclear Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nuclear Periphery Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nucleolus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nucleus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Peroxisomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Vacuole NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Vacuole Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Standard name
Human Ortholog
Description Essential nuclear envelope integral membrane protein; interacts and functions with Apq12p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA nuclear export, and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism; homologous to Brl1p
Localization
Cell Percentages nuclear periphery (27%), nucleus (9%), mixed (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Brr6

Brr6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Brr6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available