| Standard name | |
|---|---|
| Description | Essential nuclear envelope integral membrane protein; interacts and functions with Apq12p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA nuclear export, and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism; homologous to Brl1p |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 1 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 8 | 5 | 7 | 0 | 9 | 1 | 0 | 1 | 3 |
| Bud | 0 | 1 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 4 | 4 | 2 | 0 | 4 | 0 | 0 | 0 | 0 |
| Bud Neck | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 2 | 3 | 1 | 0 | 5 | 0 | 0 | 0 | 0 |
| Bud Site | 0 | 0 | – | – | – | – | – | 2 | – | – | – | – | – | – | – | 1 | 1 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 1 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 3 | 34 | – | – | – | – | – | 3 | – | – | – | – | – | – | – | 47 | 50 | 43 | 1 | 11 | 0 | 1 | 1 | 3 |
| Endoplasmic Reticulum | 0 | 8 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 6 | 2 | 4 | 0 | 9 | 1 | 1 | 2 | 5 |
| Endosome | 0 | 24 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 41 | 30 | 29 | 6 | 61 | 5 | 4 | 11 | 4 |
| Golgi | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 |
| Mitochondria | 14 | 69 | – | – | – | – | – | 14 | – | – | – | – | – | – | – | 21 | 16 | 16 | 6 | 36 | 3 | 3 | 7 | 5 |
| Nucleus | 0 | 5 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 4 | 41 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 1 | 6 | 6 | 27 | 116 | 3 | 2 | 3 | 5 |
| Nucleolus | 0 | 1 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 3 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 1 | 0 | 0 | 2 | 4 | 0 | 1 | 1 | 1 |
| SpindlePole | 0 | 2 | – | – | – | – | – | 5 | – | – | – | – | – | – | – | 14 | 9 | 6 | 2 | 13 | 2 | 2 | 1 | 1 |
| Vac/Vac Membrane | 4 | 74 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 28 | 19 | 28 | 3 | 16 | 1 | 0 | 0 | 2 |
| Unique Cell Count | 18 | 209 | 19 | 140 | 121 | 119 | 56 | 304 | 20 | 17 | 33 | 34 | ||||||||||||
| Labelled Cell Count | 25 | 264 | 27 | 176 | 148 | 146 | 56 | 304 | 20 | 17 | 33 | 34 | ||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.8 | 5.0 | – | – | – | – | – | 4.1 | – | – | – | – | – | – | – | 5.7 | 5.9 | 5.9 | – | – | – |
| Std Deviation (1e-4) | 0.6 | 0.9 | – | – | – | – | – | 1.8 | – | – | – | – | – | – | – | 1.0 | 1.1 | 1.3 | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
