Standard name
Description Essential nuclear envelope integral membrane protein; interacts and functions with Apq12p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA nuclear export, and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism; homologous to Brl1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.06 0 0.06 0 0 0.07 0 0 0.09
Bud 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.11 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.16 0.16 0.34 0.41 0.36 0 0 0 0.07 0.05 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.09 0.08 0.08 0.18
Endosome 0 0.11 0 0.29 0.25 0.24 0.11 0.2 0.28 0.25 0.35 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.78 0.33 0.74 0.15 0.13 0.13 0.11 0.12 0.18 0.18 0.22 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.22 0.2 0 0 0.05 0.05 0.49 0.38 0.17 0.16 0.11 0.16
Nucleolus 0 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0.07 0 0
SpindlePole 0 0 0.26 0.1 0.07 0.05 0 0 0.11 0.12 0 0
Vac/Vac Membrane 0.22 0.35 0.05 0.2 0.16 0.24 0.06 0.06 0.06 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 8 5 7 0 9 1 0 1 3
Bud 0 1 1 4 4 2 0 4 0 0 0 0
Bud Neck 0 0 0 2 3 1 0 5 0 0 0 0
Bud Site 0 0 2 1 1 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 34 3 47 50 43 1 11 0 1 1 3
Endoplasmic Reticulum 0 8 0 6 2 4 0 9 1 1 2 5
Endosome 0 24 0 41 30 29 6 61 5 4 11 4
Golgi 0 0 0 0 0 1 2 1 0 0 0 0
Mitochondria 14 69 14 21 16 16 6 36 3 3 7 5
Nucleus 0 5 0 1 2 1 0 2 0 0 0 0
Nuclear Periphery 4 41 0 1 6 6 27 116 3 2 3 5
Nucleolus 0 1 1 1 1 2 1 3 0 0 0 0
Peroxisomes 0 3 0 1 0 0 2 4 0 1 1 1
SpindlePole 0 2 5 14 9 6 2 13 2 2 1 1
Vac/Vac Membrane 4 74 1 28 19 28 3 16 1 0 0 2
Unique Cell Count 18 209 19 140 121 119 56 304 20 17 33 34
Labelled Cell Count 25 264 27 176 148 146 56 304 20 17 33 34


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 5.0 4.1 5.7 5.9 5.9
Std Deviation (1e-4) 0.6 0.9 1.8 1.0 1.1 1.3
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole