Standard name | |
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Human Ortholog | |
Description | Essential nuclear envelope integral membrane protein; interacts and functions with Apq12p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA nuclear export, and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism; homologous to Brl1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 8 | 5 | 7 | 0 | 9 | 1 | 0 | 1 | 3 |
Bud | 0 | 1 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 4 | 4 | 2 | 0 | 4 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 2 | 3 | 1 | 0 | 5 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | – | – | – | – | 2 | – | – | – | – | – | – | – | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 34 | – | – | – | – | – | 3 | – | – | – | – | – | – | – | 47 | 50 | 43 | 1 | 11 | 0 | 1 | 1 | 3 |
Endoplasmic Reticulum | 0 | 8 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 6 | 2 | 4 | 0 | 9 | 1 | 1 | 2 | 5 |
Endosome | 0 | 24 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 41 | 30 | 29 | 6 | 61 | 5 | 4 | 11 | 4 |
Golgi | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 14 | 69 | – | – | – | – | – | 14 | – | – | – | – | – | – | – | 21 | 16 | 16 | 6 | 36 | 3 | 3 | 7 | 5 |
Nucleus | 0 | 5 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 41 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 1 | 6 | 6 | 27 | 116 | 3 | 2 | 3 | 5 |
Nucleolus | 0 | 1 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 3 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 1 | 0 | 0 | 2 | 4 | 0 | 1 | 1 | 1 |
SpindlePole | 0 | 2 | – | – | – | – | – | 5 | – | – | – | – | – | – | – | 14 | 9 | 6 | 2 | 13 | 2 | 2 | 1 | 1 |
Vac/Vac Membrane | 4 | 74 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 28 | 19 | 28 | 3 | 16 | 1 | 0 | 0 | 2 |
Unique Cell Count | 18 | 209 | 19 | 140 | 121 | 119 | 56 | 304 | 20 | 17 | 33 | 34 | ||||||||||||
Labelled Cell Count | 25 | 264 | 27 | 176 | 148 | 146 | 56 | 304 | 20 | 17 | 33 | 34 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 5.0 | – | – | – | – | – | 4.1 | – | – | – | – | – | – | – | 5.7 | 5.9 | 5.9 | – | – | – |
Std Deviation (1e-4) | 0.6 | 0.9 | – | – | – | – | – | 1.8 | – | – | – | – | – | – | – | 1.0 | 1.1 | 1.3 | – | – | – |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1725 | 0.0197 | -0.239 | -0.0747 | 0.2194 | -0.2409 | 1.0316 | 0.6765 | 0.8439 | 0.5529 | 1.1523 | 0.5049 | 3.7709 | 3.4721 | 3.2785 | 3.6014 | 2.876 | 3.2885 |
Actin | 0.0483 | 0.0111 | 0.0082 | 0.0534 | 0.0297 | 0.0277 | 0.0566 | 0.0044 | 0.0138 | 0.0265 | 0.0457 | 0.0156 | 0.1047 | 0.0102 | 0.0171 | 0.0254 | 0.0052 | 0.0266 |
Bud | 0.0013 | 0.0008 | 0.0001 | 0.0007 | 0.0006 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 |
Bud Neck | 0.0015 | 0.0009 | 0.0022 | 0.0089 | 0.0009 | 0.0007 | 0.0031 | 0.0008 | 0.0004 | 0.0078 | 0.0113 | 0.0008 | 0.0029 | 0.0006 | 0.0016 | 0.0192 | 0.0004 | 0.0015 |
Bud Periphery | 0.002 | 0.0015 | 0.0003 | 0.0018 | 0.0007 | 0.0003 | 0.0007 | 0.0007 | 0.0007 | 0.0008 | 0.0011 | 0.0006 | 0.0008 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0003 |
Bud Site | 0.0073 | 0.0097 | 0.0004 | 0.0047 | 0.0023 | 0.0002 | 0.0082 | 0.003 | 0.001 | 0.0014 | 0.0015 | 0.0001 | 0.0228 | 0.0009 | 0.0013 | 0.0021 | 0.0001 | 0.0005 |
Cell Periphery | 0.0006 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.0009 | 0.0008 | 0.0001 | 0.0002 |
Cytoplasm | 0.0285 | 0.0396 | 0.0209 | 0.0034 | 0.0655 | 0.0117 | 0.0559 | 0.0452 | 0.0303 | 0.0363 | 0.0383 | 0.0361 | 0.0556 | 0.0301 | 0.1757 | 0.0326 | 0.037 | 0.05 |
Cytoplasmic Foci | 0.0395 | 0.0405 | 0.0428 | 0.0944 | 0.0434 | 0.0111 | 0.0281 | 0.0292 | 0.024 | 0.0316 | 0.0503 | 0.0129 | 0.0601 | 0.0162 | 0.0576 | 0.0795 | 0.0526 | 0.0212 |
Eisosomes | 0.0019 | 0.0011 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0009 | 0.0003 | 0.0007 | 0.0005 | 0.0005 | 0.0004 | 0.002 | 0.0009 | 0.0002 | 0.002 | 0.0001 | 0.0004 |
Endoplasmic Reticulum | 0.0318 | 0.0349 | 0.0428 | 0.0135 | 0.0724 | 0.0199 | 0.0345 | 0.0583 | 0.0705 | 0.0504 | 0.0197 | 0.0425 | 0.0569 | 0.025 | 0.3068 | 0.0481 | 0.0408 | 0.0589 |
Endosome | 0.1204 | 0.0312 | 0.0389 | 0.0307 | 0.0636 | 0.0356 | 0.089 | 0.0538 | 0.0588 | 0.0339 | 0.0609 | 0.0376 | 0.0345 | 0.0389 | 0.0295 | 0.0509 | 0.0431 | 0.0391 |
Golgi | 0.0252 | 0.0074 | 0.0083 | 0.0129 | 0.0569 | 0.0204 | 0.0119 | 0.0073 | 0.0098 | 0.0125 | 0.0077 | 0.0044 | 0.012 | 0.0122 | 0.0052 | 0.0103 | 0.0063 | 0.0328 |
Lipid Particles | 0.1671 | 0.0487 | 0.1686 | 0.2265 | 0.2269 | 0.1 | 0.0832 | 0.102 | 0.1361 | 0.1042 | 0.0928 | 0.0448 | 0.0378 | 0.1887 | 0.0171 | 0.1932 | 0.1329 | 0.131 |
Mitochondria | 0.0212 | 0.0281 | 0.0071 | 0.025 | 0.0137 | 0.0064 | 0.004 | 0.0239 | 0.0101 | 0.0092 | 0.0067 | 0.0059 | 0.0094 | 0.0035 | 0.002 | 0.0022 | 0.0012 | 0.0089 |
None | 0.053 | 0.1373 | 0.0695 | 0.0044 | 0.0167 | 0.0165 | 0.1293 | 0.0867 | 0.0703 | 0.0606 | 0.1181 | 0.0311 | 0.2111 | 0.1398 | 0.1059 | 0.1766 | 0.0813 | 0.0967 |
Nuclear Periphery | 0.3359 | 0.4825 | 0.4654 | 0.3837 | 0.3199 | 0.6704 | 0.3127 | 0.4049 | 0.4616 | 0.3422 | 0.318 | 0.675 | 0.1914 | 0.3195 | 0.1053 | 0.2114 | 0.4506 | 0.4305 |
Nucleolus | 0.0053 | 0.003 | 0.0033 | 0.0213 | 0.002 | 0.0014 | 0.0062 | 0.0228 | 0.0084 | 0.0093 | 0.0044 | 0.0023 | 0.0301 | 0.0054 | 0.002 | 0.0057 | 0.001 | 0.0037 |
Nucleus | 0.0261 | 0.0229 | 0.0325 | 0.0139 | 0.0304 | 0.0253 | 0.0391 | 0.0546 | 0.0392 | 0.0682 | 0.0599 | 0.0338 | 0.0452 | 0.0202 | 0.0573 | 0.0163 | 0.0145 | 0.0485 |
Peroxisomes | 0.0113 | 0.0081 | 0.0144 | 0.0226 | 0.0188 | 0.0026 | 0.0112 | 0.0063 | 0.0034 | 0.0271 | 0.0134 | 0.0016 | 0.0277 | 0.005 | 0.0171 | 0.0115 | 0.0043 | 0.0024 |
Punctate Nuclear | 0.0496 | 0.0655 | 0.0506 | 0.0554 | 0.0172 | 0.0337 | 0.1063 | 0.0687 | 0.0378 | 0.1563 | 0.1305 | 0.0337 | 0.0868 | 0.1728 | 0.0874 | 0.1024 | 0.1182 | 0.0363 |
Vacuole | 0.0089 | 0.0062 | 0.0042 | 0.0078 | 0.005 | 0.0029 | 0.0098 | 0.0101 | 0.0065 | 0.009 | 0.007 | 0.0036 | 0.0032 | 0.0025 | 0.007 | 0.0032 | 0.0022 | 0.0038 |
Vacuole Periphery | 0.0135 | 0.0186 | 0.0191 | 0.0141 | 0.0127 | 0.0126 | 0.0084 | 0.0162 | 0.0162 | 0.0118 | 0.0113 | 0.0168 | 0.0037 | 0.007 | 0.0028 | 0.0058 | 0.0079 | 0.0064 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.1455 | 1.6606 | 6.1611 | 12.7669 | 8.4295 | 9.2828 | 10.4937 | 17.5647 | 9.4644 | 14.715 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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