Standard name | |
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Description | Processivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 7 | 3 |
Bud | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 3 | 3 | 3 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 5 | 3 | 11 | 9 |
Bud Neck | 0 | 0 | 1 | 3 | 4 | 9 | 7 | 8 | 3 | 8 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 6 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 7 | 9 | 7 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 58 | 90 | 133 | 100 | 104 | 129 | 115 | 118 | 96 | 149 | 119 | 149 | 40 | 90 | 91 | 56 | 49 | 41 | 56 | 73 | 84 | 48 | 93 | 80 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 10 | 11 | 3 | 0 | 1 | 1 | 0 | 1 | 1 |
Endosome | 3 | 0 | 7 | 5 | 9 | 11 | 6 | 1 | 0 | 1 | 0 | 0 | 4 | 5 | 4 | 14 | 8 | 14 | 3 | 2 | 3 | 1 | 3 | 4 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 8 | 2 |
Mitochondria | 27 | 87 | 6 | 22 | 16 | 98 | 113 | 88 | 294 | 270 | 235 | 239 | 1 | 5 | 4 | 6 | 4 | 1 | 7 | 15 | 22 | 7 | 27 | 20 |
Nucleus | 97 | 51 | 128 | 123 | 161 | 174 | 147 | 164 | 140 | 166 | 106 | 96 | 78 | 103 | 109 | 39 | 37 | 34 | 122 | 93 | 176 | 55 | 105 | 79 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 1 | 1 | 0 | 1 | 3 | 2 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 0 | 2 | 2 | 4 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
SpindlePole | 0 | 1 | 4 | 10 | 17 | 16 | 13 | 8 | 4 | 10 | 4 | 14 | 0 | 1 | 1 | 0 | 0 | 0 | 11 | 8 | 28 | 10 | 20 | 27 |
Vac/Vac Membrane | 48 | 33 | 37 | 17 | 34 | 24 | 23 | 25 | 9 | 11 | 18 | 22 | 18 | 14 | 23 | 71 | 73 | 46 | 42 | 53 | 73 | 29 | 63 | 53 |
Unique Cell Count | 210 | 220 | 301 | 257 | 319 | 402 | 372 | 366 | 413 | 514 | 375 | 411 | 133 | 211 | 224 | 163 | 154 | 126 | 261 | 264 | 418 | 173 | 367 | 308 |
Labelled Cell Count | 234 | 263 | 319 | 281 | 347 | 474 | 432 | 420 | 558 | 631 | 499 | 544 | 141 | 219 | 234 | 198 | 182 | 142 | 261 | 264 | 418 | 173 | 367 | 308 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.4 | 3.5 | 5.3 | 4.9 | 5.7 | 4.4 | 4.6 | 4.7 | 3.2 | 3.7 | 3.6 | 3.6 | 5.2 | 5.1 | 5.1 | 6.5 | 8.0 | 9.2 | 5.8 | 6.2 | 6.0 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.3 | 1.4 | 2.4 | 1.2 | 2.2 | 1.5 | 0.7 | 0.8 | 1.5 | 1.4 | 1.4 | 1.2 | 1.3 | 1.5 | 1.9 | 2.6 | 2.4 | 2.5 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.1 | -0.28 | -0.22 | -0.18 | -0.72 | -0.53 | -0.57 | -0.55 | -0.02 | -0.06 | -0.07 | 0.3 | 0.59 | 0.79 | 0.13 | 0.23 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 7.6 | 8.3 | 5.8 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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