Standard name
Human Ortholog
Description Processivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.28 0.41 0.44 0.39 0.33 0.32 0.31 0.32 0.23 0.29 0.32 0.36 0.3 0.43 0.41 0.34 0.32 0.33 0.22 0.28 0.2 0.28 0.25 0.26
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.4 0 0.09 0.05 0.24 0.3 0.24 0.71 0.53 0.63 0.58 0 0 0 0 0 0 0 0.06 0.05 0 0.08 0.07
Nucleus 0.46 0.23 0.43 0.48 0.5 0.43 0.4 0.45 0.34 0.32 0.28 0.23 0.59 0.49 0.49 0.24 0.24 0.27 0.47 0.35 0.42 0.32 0.29 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0.09
Vac/Vac Membrane 0.23 0.15 0.12 0.07 0.11 0.06 0.06 0.07 0 0 0.05 0.05 0.14 0.07 0.1 0.44 0.47 0.37 0.16 0.2 0.18 0.17 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 7 3
Bud 0 0 0 0 1 5 3 3 3 3 6 8 0 0 0 0 0 0 4 2 5 3 11 9
Bud Neck 0 0 1 3 4 9 7 8 3 8 2 7 0 0 0 0 0 0 0 1 1 2 6 5
Bud Site 0 0 0 1 0 2 0 2 7 9 7 7 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0
Cytoplasm 58 90 133 100 104 129 115 118 96 149 119 149 40 90 91 56 49 41 56 73 84 48 93 80
Endoplasmic Reticulum 0 0 1 0 0 2 2 0 0 1 0 0 0 0 0 10 11 3 0 1 1 0 1 1
Endosome 3 0 7 5 9 11 6 1 0 1 0 0 4 5 4 14 8 14 3 2 3 1 3 4
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 1 1 8 2
Mitochondria 27 87 6 22 16 98 113 88 294 270 235 239 1 5 4 6 4 1 7 15 22 7 27 20
Nucleus 97 51 128 123 161 174 147 164 140 166 106 96 78 103 109 39 37 34 122 93 176 55 105 79
Nuclear Periphery 1 0 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 1 1 0 1 3 2 2 1 2 1 2 0 0 0 1 0 2 4 0 2 2 4 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0
SpindlePole 0 1 4 10 17 16 13 8 4 10 4 14 0 1 1 0 0 0 11 8 28 10 20 27
Vac/Vac Membrane 48 33 37 17 34 24 23 25 9 11 18 22 18 14 23 71 73 46 42 53 73 29 63 53
Unique Cell Count 210 220 301 257 319 402 372 366 413 514 375 411 133 211 224 163 154 126 261 264 418 173 367 308
Labelled Cell Count 234 263 319 281 347 474 432 420 558 631 499 544 141 219 234 198 182 142 261 264 418 173 367 308


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.5 5.3 4.9 5.7 4.4 4.6 4.7 3.2 3.7 3.6 3.6 5.2 5.1 5.1 6.5 8.0 9.2 5.8 6.2 6.0
Std Deviation (1e-4) 0.7 0.9 1.3 1.4 2.4 1.2 2.2 1.5 0.7 0.8 1.5 1.4 1.4 1.2 1.3 1.5 1.9 2.6 2.4 2.5 2.1
Intensity Change (Log2) -0.11 0.1 -0.28 -0.22 -0.18 -0.72 -0.53 -0.57 -0.55 -0.02 -0.06 -0.07 0.3 0.59 0.79 0.13 0.23 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 7.6 8.3 5.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5695 2.6685 2.4658 2.0952 1.5891 3.021 4.421 3.5442 3.1375 3.449 4.4169 3.5737 2.1979 1.2316 1.3118 0.4816 1.2791 1.3088
Actin 0.0433 0.0004 0.0004 0.0015 0.0002 0.0105 0.0416 0.0018 0.0086 0.0002 0.0089 0.0025 0.0231 0.0049 0.0024 0.0013 0.0003 0.0075
Bud 0.0006 0.0002 0.0001 0.0004 0.0002 0.0005 0.001 0.0026 0.0007 0 0.0011 0.0002 0.0004 0.0016 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0099 0.0009 0.0005 0.0015 0.0013 0.0171 0.0016 0.0006 0.0007 0.0003 0.0028 0.003 0.0071 0.0009 0.0003 0.0005 0.006 0.0038
Bud Periphery 0.0007 0.0001 0 0.0002 0.0004 0.0008 0.0022 0.0064 0.0007 0 0.0021 0.0006 0.0006 0.0038 0.0002 0.0001 0.0001 0.0001
Bud Site 0.004 0.0023 0.0004 0.0007 0.0006 0.0015 0.0075 0.0064 0.0024 0.0001 0.0019 0.0003 0.0064 0.0046 0.0004 0.0003 0.0004 0.0003
Cell Periphery 0.0004 0.0002 0.0001 0 0.0011 0.0005 0.0005 0.0002 0.0001 0 0.0002 0.0001 0.0006 0.0003 0.0001 0 0.0001 0
Cytoplasm 0.1466 0.1574 0.0761 0.1669 0.2356 0.1399 0.1351 0.1652 0.1227 0.079 0.1313 0.1664 0.2288 0.1402 0.1055 0.2502 0.2057 0.2057
Cytoplasmic Foci 0.0276 0.0037 0.0248 0.0022 0.0027 0.0214 0.068 0.0142 0.0203 0.0037 0.0108 0.0071 0.0409 0.0031 0.0037 0.0035 0.0033 0.0036
Eisosomes 0.0003 0.0001 0 0 0 0.0002 0.0008 0.0001 0.0001 0 0.0005 0.0001 0.001 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0094 0.0123 0.0096 0.0053 0.0021 0.0051 0.013 0.0071 0.0033 0.0011 0.0127 0.0036 0.0181 0.0069 0.0065 0.0026 0.0083 0.0019
Endosome 0.0204 0.0038 0.0046 0.0025 0.0028 0.0208 0.0384 0.0055 0.017 0.0013 0.0181 0.0051 0.0199 0.0037 0.013 0.0028 0.0084 0.0027
Golgi 0.0089 0.0001 0.0005 0.0001 0.0001 0.0153 0.0292 0.0018 0.0048 0 0.0163 0.0004 0.0038 0.0008 0.0112 0.0001 0.0002 0.0014
Lipid Particles 0.0105 0.0008 0.0039 0.0001 0.0002 0.0051 0.0169 0.0056 0.0117 0.001 0.0087 0.0014 0.0127 0.0007 0.0036 0.0001 0.0002 0.0032
Mitochondria 0.01 0.0005 0.0008 0.0012 0.0004 0.0035 0.0231 0.0042 0.0032 0.0006 0.06 0.0145 0.0025 0.002 0.0057 0.0015 0.0013 0.0007
None 0.257 0.1794 0.145 0.109 0.1625 0.2131 0.2654 0.385 0.2458 0.2322 0.3961 0.2121 0.1488 0.3021 0.3255 0.3774 0.2351 0.2537
Nuclear Periphery 0.0165 0.0269 0.0193 0.0314 0.0072 0.0164 0.0235 0.0383 0.0182 0.0086 0.0286 0.0144 0.0635 0.0168 0.0324 0.0123 0.0177 0.0071
Nucleolus 0.0048 0.0053 0.0033 0.0022 0.004 0.0086 0.003 0.0064 0.0038 0.0022 0.006 0.0075 0.0144 0.0053 0.0016 0.0019 0.0051 0.0028
Nucleus 0.3542 0.5727 0.6887 0.6426 0.5123 0.4536 0.2475 0.277 0.491 0.6484 0.2547 0.498 0.3314 0.4679 0.4738 0.3246 0.4851 0.4613
Peroxisomes 0.0229 0.0001 0.0069 0.0002 0.0001 0.0088 0.0444 0.0307 0.0138 0.0001 0.0078 0.0115 0.0241 0.0003 0.0007 0.0004 0.0001 0.004
Punctate Nuclear 0.0461 0.0288 0.0129 0.0307 0.029 0.0505 0.028 0.0366 0.0279 0.0204 0.0263 0.0486 0.0421 0.0314 0.0097 0.019 0.018 0.0386
Vacuole 0.0047 0.0034 0.0015 0.001 0.0367 0.0051 0.0058 0.0033 0.0023 0.0006 0.0038 0.002 0.0067 0.002 0.0019 0.0009 0.0041 0.0011
Vacuole Periphery 0.001 0.0004 0.0004 0.0004 0.0006 0.0017 0.0035 0.0009 0.0007 0.0001 0.0014 0.0005 0.0028 0.0005 0.0013 0.0003 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2070 1258 1753 1926 2029 1868 2074 1431 4099 3126 3827 3357

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.05 761.57 912.36 858.61 654.80 818.83 853.31 900.67 669.57 795.79 880.36 876.54
Standard Deviation 104.32 190.33 119.56 148.33 96.80 100.67 128.22 130.20 101.73 146.36 127.76 142.42
Intensity Change Log 2 0.154875 0.415501 0.327901 0.322510 0.382016 0.459944 0.239295 0.399221 0.393991

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000036 0.000421 0.000500 0.002142 0.000036 0.000179 0.000263 0.003422 0.000036 0.000276 0.000371 0.002687
Bud Neck 0.003856 0.010577 0.009762 0.018220 0.003155 0.005887 0.006190 0.018337 0.003509 0.007775 0.007826 0.018270
Bud Site 0.000642 0.004423 0.002336 0.030693 0.000788 0.003772 0.004294 0.026107 0.000714 0.004034 0.003397 0.028738
Cell Periphery 0.000143 0.000185 0.000099 0.000483 0.000120 0.000148 0.000358 0.000285 0.000132 0.000163 0.000239 0.000399
Cytoplasm 0.279893* 0.265904* 0.039225 0.184676* 0.254403* 0.215350* 0.103621* 0.138489* 0.267275* 0.235695* 0.074124 0.164988*
Cytoplasmic Foci 0.043862 0.064210 0.000403 0.012314 0.034086 0.036342 0.004426 0.005255 0.039023 0.047557 0.002583 0.009305
Eisosomes 0.000083 0.000053 0.000020 0.000036 0.000033 0.000041 0.000043 0.000041 0.000058 0.000046 0.000032 0.000038
Endoplasmic Reticulum 0.000819 0.002731 0.006073 0.004218 0.000891 0.001113 0.002580 0.008265 0.000855 0.001764 0.004180 0.005943
Endosome 0.002010 0.005828 0.000598 0.008423 0.001208 0.003075 0.001113 0.006492 0.001613 0.004183 0.000877 0.007600
Golgi 0.000702 0.001762 0.000071 0.005831 0.000199 0.001505 0.000402 0.001684 0.000453 0.001608 0.000250 0.004063
Lipid Particles 0.004007 0.006796 0.000335 0.001130 0.003270 0.004566 0.001224 0.000963 0.003642 0.005463 0.000816 0.001059
Mitochondria 0.002569 0.007848 0.001369 0.005159 0.002453 0.008047 0.001726 0.006296 0.002512 0.007967 0.001563 0.005644
Mitotic Spindle 0.002306 0.007371 0.005733 0.058274 0.000803 0.007497 0.005143 0.039577 0.001562 0.007446 0.005413 0.050304
None 0.021538 0.010627 0.006594 0.013985 0.016201 0.009781 0.019563 0.010843 0.018896 0.010121 0.013622 0.012646
Nuclear Periphery 0.001668 0.001854 0.001946 0.002963 0.001294 0.001604 0.001390 0.002771 0.001483 0.001705 0.001645 0.002881
Nuclear Periphery Foci 0.000470 0.000991 0.000462 0.001736 0.000436 0.000487 0.000327 0.001487 0.000453 0.000689 0.000388 0.001630
Nucleolus 0.003136 0.005103 0.003290 0.003833 0.003483 0.004744 0.003338 0.003784 0.003308 0.004888 0.003316 0.003812
Nucleus 0.624648* 0.583471* 0.916439* 0.618196* 0.670845* 0.684061* 0.837372* 0.690551* 0.647516* 0.643581* 0.873589* 0.649039*
Peroxisomes 0.001694 0.006056 0.000124 0.001519 0.001209 0.004266 0.000710 0.001176 0.001454 0.004986 0.000442 0.001373
Vacuole 0.005686 0.012133 0.004489 0.022039 0.004837 0.006767 0.005692 0.030188 0.005265 0.008926 0.005141 0.025513
Vacuole Periphery 0.000233 0.001656 0.000135 0.004129 0.000251 0.000767 0.000225 0.003986 0.000242 0.001125 0.000184 0.004068

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.47 -19.13 -3.39 -2.28 -1.36 -5.00 -16.29 -2.69 -2.47 -2.03 -5.05 -24.97 -4.20 -3.49 -2.54
Bud Neck -5.16 -11.12 -13.59 -4.96 -2.75 -3.89 -9.77 -12.00 -9.11 -6.05 -6.33 -14.70 -18.24 -10.72 -6.60
Bud Site -4.55 -5.89 -9.78 -6.87 -7.95 -3.70 -4.73 -8.26 -5.95 -5.86 -5.71 -7.04 -12.74 -9.15 -9.73
Cell Periphery -2.01 3.14 -4.94 -3.43 -6.34 -2.40 -5.69 -3.19 -1.67 2.68 -2.75 -3.90 -5.88 -4.24 -2.80
Cytoplasm 1.51 41.62 19.49 14.56 -22.91 4.84 25.89 19.47 14.21 -5.02 5.22 46.04 27.05 19.33 -18.29
Cytoplasmic Foci -4.39 19.11 15.68 16.27 -8.18 -0.76 16.26 16.20 16.38 -0.04 -3.15 24.90 22.01 22.38 -5.90
Eisosomes 1.65 3.18 1.82 0.81 -9.24 -3.84 -3.99 -7.39 -3.38 -2.89 1.24 2.46 0.70 -2.11 -7.49
Endoplasmic Reticulum -7.40 -18.13 -13.80 -7.25 3.61 -1.73 -14.93 -14.63 -14.30 -7.72 -6.99 -23.38 -19.99 -16.55 -3.44
Endosome -3.83 4.74 -2.63 1.80 -5.15 -4.39 0.24 -3.22 -0.26 -3.27 -5.32 3.91 -4.19 0.94 -5.93
Golgi -2.56 3.12 -2.65 -0.61 -4.12 -2.86 -0.69 -2.28 1.71 -1.40 -3.54 1.91 -3.33 0.23 -4.21
Lipid Particles -3.34 9.20 7.14 8.01 -6.97 -2.70 6.31 6.96 8.75 -0.08 -4.05 10.88 9.82 11.68 -3.25
Mitochondria -3.55 1.52 -1.73 2.91 -5.55 -4.10 0.62 -1.81 3.19 -3.08 -5.41 1.41 -2.52 4.35 -5.50
Mitotic Spindle -2.64 -3.83 -13.37 -11.26 -11.18 -4.34 -5.08 -10.70 -8.60 -8.94 -4.82 -6.09 -17.21 -14.16 -14.44
None 4.61 7.52 4.47 -0.80 -6.10 3.79 0.71 4.55 0.62 3.95 6.07 5.63 6.11 -0.59 -0.15
Nuclear Periphery -0.85 -4.09 -9.62 -10.97 -7.82 -2.05 -7.40 -15.67 -12.54 -9.20 -1.53 -6.97 -16.25 -16.45 -11.90
Nuclear Periphery Foci -2.02 -1.68 -4.83 -1.30 -3.87 -0.20 -1.47 -4.43 -4.38 -3.45 -1.75 -2.02 -6.43 -4.69 -4.89
Nucleolus -3.27 -4.23 -3.85 -0.55 1.06 -1.52 -2.64 -0.67 0.95 2.18 -3.26 -4.90 -3.36 -0.11 1.91
Nucleus 3.55 -36.34 -4.87 -7.59 29.67 -1.30 -21.83 -3.81 -2.49 15.16 0.54 -40.00 -5.76 -5.75 30.80
Peroxisomes -4.87 6.61 4.03 6.37 -3.05 -4.00 2.42 2.13 5.24 -0.39 -6.09 5.81 4.24 8.06 -1.99
Vacuole -7.20 -2.31 -15.98 -12.70 -15.00 -2.82 -4.69 -15.39 -14.71 -13.78 -6.87 -5.08 -22.19 -19.90 -20.25
Vacuole Periphery -2.43 1.88 -3.26 0.08 -3.46 -3.54 0.66 -2.31 -1.25 -2.40 -3.53 1.47 -3.97 -0.98 -4.16