Standard name
Description Processivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.28 0.41 0.44 0.39 0.33 0.32 0.31 0.32 0.23 0.29 0.32 0.36 0.3 0.43 0.41 0.34 0.32 0.33 0.22 0.28 0.2 0.28 0.25 0.26
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.4 0 0.09 0.05 0.24 0.3 0.24 0.71 0.53 0.63 0.58 0 0 0 0 0 0 0 0.06 0.05 0 0.08 0.07
Nucleus 0.46 0.23 0.43 0.48 0.5 0.43 0.4 0.45 0.34 0.32 0.28 0.23 0.59 0.49 0.49 0.24 0.24 0.27 0.47 0.35 0.42 0.32 0.29 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0.09
Vac/Vac Membrane 0.23 0.15 0.12 0.07 0.11 0.06 0.06 0.07 0 0 0.05 0.05 0.14 0.07 0.1 0.44 0.47 0.37 0.16 0.2 0.18 0.17 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 7 3
Bud 0 0 0 0 1 5 3 3 3 3 6 8 0 0 0 0 0 0 4 2 5 3 11 9
Bud Neck 0 0 1 3 4 9 7 8 3 8 2 7 0 0 0 0 0 0 0 1 1 2 6 5
Bud Site 0 0 0 1 0 2 0 2 7 9 7 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0
Cytoplasm 58 90 133 100 104 129 115 118 96 149 119 149 40 90 91 56 49 41 56 73 84 48 93 80
Endoplasmic Reticulum 0 0 1 0 0 2 2 0 0 1 0 0 0 0 0 10 11 3 0 1 1 0 1 1
Endosome 3 0 7 5 9 11 6 1 0 1 0 0 4 5 4 14 8 14 3 2 3 1 3 4
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 1 1 8 2
Mitochondria 27 87 6 22 16 98 113 88 294 270 235 239 1 5 4 6 4 1 7 15 22 7 27 20
Nucleus 97 51 128 123 161 174 147 164 140 166 106 96 78 103 109 39 37 34 122 93 176 55 105 79
Nuclear Periphery 1 0 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 1 1 0 1 3 2 2 1 2 1 2 0 0 0 1 0 2 4 0 2 2 4 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0
SpindlePole 0 1 4 10 17 16 13 8 4 10 4 14 0 1 1 0 0 0 11 8 28 10 20 27
Vac/Vac Membrane 48 33 37 17 34 24 23 25 9 11 18 22 18 14 23 71 73 46 42 53 73 29 63 53
Unique Cell Count 210 220 301 257 319 402 372 366 413 514 375 411 133 211 224 163 154 126 261 264 418 173 367 308
Labelled Cell Count 234 263 319 281 347 474 432 420 558 631 499 544 141 219 234 198 182 142 261 264 418 173 367 308


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.5 5.3 4.9 5.7 4.4 4.6 4.7 3.2 3.7 3.6 3.6 5.2 5.1 5.1 6.5 8.0 9.2 5.8 6.2 6.0
Std Deviation (1e-4) 0.7 0.9 1.3 1.4 2.4 1.2 2.2 1.5 0.7 0.8 1.5 1.4 1.4 1.2 1.3 1.5 1.9 2.6 2.4 2.5 2.1
Intensity Change (Log2) – – – -0.11 0.1 -0.28 -0.22 -0.18 -0.72 -0.53 -0.57 -0.55 -0.02 -0.06 -0.07 0.3 0.59 0.79 0.13 0.23 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.6 8.3 5.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Doc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available