Standard name
Human Ortholog
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functionally complemented by human NFYA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 – 0.05 0 0.08 0.05 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0.05 – 0 0 0.06 – 0.05 0 0 0 0.12 0.16 0.2 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.19 0.28 0 – 0 0 0.1 – 0.27 0.29 0.29 0.23 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.92 0.87 0.94 – 0.98 0.95 0.89 – 0.87 0.79 0.78 0.79 0.88 0.83 0.77 – – – 0.88 0.84 0.84 0.87 0.82 0.69
Nuclear Periphery 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 – 0.1 0.12 0.26 0.23 0 0 0 – – – 0 0 0 0 0 0.05
Peroxisomes 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0.08 0 0.05 0.09 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 1 0 0 0 1
Bud 0 0 0 – 1 0 0 – 0 1 1 0 0 0 0 – – – 2 0 1 2 0 3
Bud Neck 0 1 0 – 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1
Bud Site 0 1 0 – 0 2 1 – 5 2 8 5 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 – 0 0 0 – 0 0 0 0 1 0 0 – – – 0 0 0 0 0 0
Cytoplasm 1 4 4 – 1 1 11 – 5 5 1 3 42 64 88 – – – 1 4 0 1 5 5
Endoplasmic Reticulum 0 1 0 – 0 0 0 – 0 0 0 0 0 1 1 – – – 0 0 0 0 0 1
Endosome 0 1 0 – 0 1 0 – 0 0 0 0 0 2 4 – – – 1 1 0 0 3 6
Golgi 0 0 0 – 0 0 0 – 0 0 0 0 1 0 1 – – – 0 0 0 0 1 0
Mitochondria 20 47 1 – 0 2 18 – 29 34 30 25 2 0 1 – – – 2 3 0 2 1 7
Nucleus 97 144 72 – 83 122 157 – 94 92 81 84 309 341 336 – – – 105 148 72 185 279 267
Nuclear Periphery 0 3 0 – 0 1 3 – 2 1 0 2 0 0 0 – – – 0 0 0 0 1 1
Nucleolus 2 3 0 – 1 3 5 – 11 14 27 25 0 0 4 – – – 0 0 1 4 9 20
Peroxisomes 0 0 0 – 0 0 0 – 0 0 0 0 0 2 2 – – – 0 0 0 0 0 0
SpindlePole 0 0 1 – 0 1 2 – 0 0 1 0 1 3 4 – – – 0 0 1 1 3 5
Vac/Vac Membrane 0 3 1 – 0 1 2 – 0 0 1 0 1 3 7 – – – 4 13 3 11 31 55
Unique Cell Count 105 166 77 85 128 177 108 117 104 107 353 410 436 120 176 86 212 342 387
Labelled Cell Count 120 208 79 86 134 199 146 149 150 144 357 416 448 120 176 86 212 342 387


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.1 6.0 – 6.3 5.9 5.5 – 5.3 4.9 5.1 5.3 6.5 6.9 6.9 – – – 6.9 7.2 7.4
Std Deviation (1e-4) 0.7 5.4 1.1 – 0.8 1.1 1.4 – 1.2 1.0 1.4 1.5 1.3 1.5 2.2 – – – 1.4 1.7 1.8
Intensity Change (Log2) – – – – 0.08 -0.03 -0.13 – -0.17 -0.3 -0.24 -0.19 0.12 0.2 0.2 – – – 0.21 0.26 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud – 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Neck – 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Site – 0 0 0 – 0 0 0 0 0 0 0 – – –
Cell Periphery – 0 0 0 – 0 0 0 0 0 0 0 – – –
Cytoplasm – 0 0 0 – 0 0 0 0 1.7 2.4 3.2 – – –
Endoplasmic Reticulum – 0 0 0 – 0 0 0 0 0 0 0 – – –
Endosome – 0 0 0 – 0 0 0 0 0 0 0 – – –
Golgi – 0 0 0 – 0 0 0 0 0 0 0 – – –
Mitochondria – 0 0 2.5 – 4.6 0 4.9 4.2 0 0 0 – – –
Nucleus – 1.3 0.6 -1.2 – -1.4 -2.8 -2.9 -2.8 -1.5 -2.3 -3.3 – – –
Nuclear Periphery – 0 0 0 – 0 0 0 0 0 0 0 – – –
Nucleolus – 0 0 0 – 0 0 4.8 4.6 0 0 0 – – –
Peroxisomes – 0 0 0 – 0 0 0 0 0 0 0 – – –
SpindlePole – 0 0 0 – 0 0 0 0 0 0 0 – – –
Vacuole – 0 0 0 – 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7396 1.5246 1.3781 1.3975 1.1355 1.5299 5.7084 5.1886 5.3343 5.0059 5.1836 5.3762 3.0844 2.4271 2.6348 1.9115 2.1852 2.4511
Actin 0.0195 0 0.0023 0 0 0.0002 0.0068 0 0.0148 0.0044 0.0035 0.0001 0.0235 0 0.0083 0 0.022 0.0001
Bud 0.0001 0 0 0 0 0 0.0005 0 0.0008 0.001 0.0004 0 0.0002 0 0.0002 0 0.0004 0.0001
Bud Neck 0.0001 0.0002 0.0001 0 0.0002 0.001 0.0031 0.0001 0.0031 0.0002 0.004 0.0008 0.0022 0.0001 0.0007 0.0001 0.0025 0.0011
Bud Periphery 0.0002 0 0 0 0 0.0001 0.001 0 0.0015 0.0033 0.0011 0.0001 0.0002 0 0.0003 0 0.0005 0.0001
Bud Site 0.0002 0.0001 0.0001 0 0 0.0002 0.0043 0.0001 0.0079 0.0031 0.0009 0.0001 0.0013 0.0001 0.0007 0 0.0058 0.0002
Cell Periphery 0 0 0 0 0 0 0.0004 0 0.0005 0.0002 0.0003 0 0.0001 0 0.0001 0 0.0002 0
Cytoplasm 0.0019 0.0005 0.0002 0 0.0002 0.0001 0.0055 0.0023 0.0033 0.0012 0.0024 0.0002 0.0189 0.0006 0.005 0.0001 0.0027 0.0018
Cytoplasmic Foci 0.0015 0.0013 0.0006 0 0 0.0009 0.0063 0 0.0039 0.0005 0.0067 0 0.0228 0 0.0047 0 0.0115 0.0001
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0009 0 0 0 0 0.0001 0.0051 0.0001 0.0028 0.0036 0.0017 0.0001 0.0037 0.0001 0.0016 0 0.0015 0.0002
Endosome 0.0041 0.0003 0.0002 0 0 0.0011 0.0268 0.0002 0.0136 0.0046 0.0173 0.0004 0.02 0.0001 0.0112 0 0.0095 0.0009
Golgi 0.0015 0.0001 0.0003 0 0 0.0002 0.0103 0 0.0057 0.0016 0.0072 0 0.004 0 0.0039 0 0.0032 0
Lipid Particles 0.0036 0.0001 0.0022 0 0 0.0022 0.0163 0 0.0064 0.001 0.0205 0.0002 0.0067 0 0.0049 0 0.0091 0.0001
Mitochondria 0.0011 0.0001 0.0003 0 0.0005 0.0003 0.0203 0.0002 0.017 0.0166 0.0044 0.0005 0.0011 0.0001 0.0019 0.0001 0.0016 0.0002
None 0.0014 0.0001 0.0001 0 0.0001 0.0002 0.0027 0.0022 0.0006 0.0003 0.0006 0.0001 0.0149 0.0001 0.0086 0 0.0017 0.0013
Nuclear Periphery 0.0064 0.0011 0.0017 0.0003 0.0021 0.0012 0.0479 0.0034 0.0058 0.0024 0.0372 0.0033 0.0112 0.0045 0.0039 0.0006 0.0168 0.0026
Nucleolus 0.0065 0.0118 0.006 0.0081 0.0373 0.0248 0.0207 0.0081 0.0062 0.0034 0.0158 0.0188 0.009 0.0101 0.0081 0.0049 0.0181 0.017
Nucleus 0.9462 0.9787 0.9815 0.9908 0.9584 0.9624 0.7988 0.9794 0.8864 0.9499 0.8426 0.9734 0.828 0.9826 0.9225 0.9938 0.8696 0.9688
Peroxisomes 0.0013 0.0001 0.003 0 0 0.0005 0.0053 0 0.0038 0.0007 0.0014 0 0.011 0 0.0031 0 0.0052 0
Punctate Nuclear 0.0016 0.0053 0.0015 0.0007 0.0009 0.0043 0.0092 0.0024 0.0108 0.0005 0.0257 0.0015 0.0196 0.0014 0.0082 0.0003 0.0159 0.0047
Vacuole 0.0009 0.0001 0 0 0.0001 0.0002 0.0058 0.0011 0.0034 0.0012 0.0036 0.0002 0.0009 0.0001 0.0014 0 0.0015 0.0007
Vacuole Periphery 0.0007 0 0 0 0.0001 0.0001 0.0025 0.0004 0.0014 0.0005 0.0029 0.0002 0.0004 0.0001 0.0005 0 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2963 9.8885 12.8409 9.5032 8.0674 9.6736 13.4351 10.544 10.7876 11.1738
Translational Efficiency 1.9042 2.2117 1.2086 1.5896 2.1842 2.0509 1.2526 2.0353 1.7213 1.5821

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
53 1384 2094 926 2184 2213 2118 1641 2237 3597 4212 2567

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 904.92 736.03 922.43 1007.51 744.26 879.62 929.46 958.26 748.07 824.37 925.97 976.03
Standard Deviation 152.79 138.33 117.94 138.12 91.57 110.70 113.23 145.39 96.62 140.65 115.65 144.76
Intensity Change Log 2 — -0.298026 0.027649 0.154932 — 0.241074 0.320586 0.364610 — -0.029634 0.167250 0.253346

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001305 0.000438 0.000483 0.001361 0.000062 0.000125 0.000379 0.000703 0.000091 0.000246 0.000431 0.000940
Bud Neck 0.007991 0.008222 0.016901 0.025138 0.002210 0.005666 0.024927 0.021418 0.002347 0.006650 0.020937 0.022760
Bud Site 0.004319 0.002364 0.001492 0.003871 0.000389 0.000818 0.001740 0.002451 0.000482 0.001413 0.001617 0.002963
Cell Periphery 0.000103 0.000225 0.000091 0.000112 0.000056 0.000111 0.000090 0.000094 0.000057 0.000155 0.000090 0.000100
Cytoplasm 0.006654 0.028149 0.004694 0.007840 0.008970 0.007396 0.004110 0.004254 0.008915 0.015381 0.004401 0.005548
Cytoplasmic Foci 0.007896 0.003654 0.000085 0.003678 0.000520 0.000887 0.000050 0.000163 0.000695 0.001952 0.000068 0.001431
Eisosomes 0.000056 0.000024 0.000023 0.000030 0.000007 0.000012 0.000023 0.000025 0.000008 0.000016 0.000023 0.000026
Endoplasmic Reticulum 0.022017 0.004785 0.004362 0.007134 0.001560 0.002550 0.004108 0.005853 0.002044 0.003410 0.004234 0.006315
Endosome 0.004230 0.001440 0.000295 0.003417 0.000136 0.001165 0.000272 0.000331 0.000233 0.001271 0.000284 0.001444
Golgi 0.001344 0.001119 0.000101 0.001486 0.000071 0.000223 0.000050 0.000771 0.000101 0.000568 0.000075 0.001029
Lipid Particles 0.005855 0.000750 0.000072 0.000358 0.000089 0.000328 0.000051 0.000156 0.000226 0.000491 0.000062 0.000229
Mitochondria 0.004526 0.002413 0.001229 0.006865 0.000364 0.001889 0.001393 0.002617 0.000462 0.002090 0.001311 0.004149
Mitotic Spindle 0.036242 0.001700 0.002399 0.022169 0.000362 0.002306 0.004020 0.004679 0.001212 0.002073 0.003214 0.010988
None 0.002019 0.029573 0.003086 0.006946 0.003542 0.002384 0.002678 0.003671 0.003506 0.012846 0.002881 0.004852
Nuclear Periphery 0.007877 0.000965 0.000583 0.002012 0.000367 0.000917 0.000831 0.000739 0.000545 0.000936 0.000708 0.001198
Nuclear Periphery Foci 0.033985 0.000745 0.000126 0.000583 0.000091 0.000125 0.000074 0.000143 0.000894 0.000364 0.000100 0.000302
Nucleolus 0.029093 0.013333 0.004827 0.003879 0.003937 0.004302 0.003322 0.004010 0.004533 0.007777 0.004070 0.003963
Nucleus 0.803861* 0.896436* 0.957261* 0.893751* 0.976728* 0.966002* 0.950569* 0.944415* 0.972632* 0.939235* 0.953896* 0.926139*
Peroxisomes 0.002126 0.001169 0.000155 0.000331 0.000073 0.000997 0.000070 0.000156 0.000121 0.001063 0.000113 0.000219
Vacuole 0.014450 0.001786 0.001526 0.006301 0.000404 0.001524 0.001120 0.003071 0.000737 0.001625 0.001322 0.004236
Vacuole Periphery 0.004050 0.000711 0.000206 0.002737 0.000064 0.000271 0.000122 0.000282 0.000158 0.000440 0.000164 0.001168

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.99 1.73 -0.24 -7.83 -6.94 -3.52 -22.40 -22.27 -18.27 -9.73 -7.32 -20.31 -18.06 -14.99 -10.21
Bud Neck 0.00 -2.65 -4.53 -9.31 -4.14 -5.00 -19.91 -14.02 -11.29 1.22 -7.85 -25.03 -18.37 -14.57 -2.01
Bud Site 1.11 1.47 -0.30 -2.94 -4.37 -2.25 -6.66 -5.37 -4.45 -2.12 -3.31 -6.77 -7.72 -5.03 -4.51
Cell Periphery -3.39 0.75 0.26 5.37 -1.31 -1.46 -3.09 -3.92 0.48 -1.24 -3.85 -3.33 -4.56 2.37 -1.57
Cytoplasm -5.08 0.89 0.34 8.71 -1.68 0.94 4.03 3.39 2.61 -0.48 -4.36 4.04 3.10 8.50 -1.29
Cytoplasmic Foci 0.79 1.51 1.27 2.44 -2.27 -1.70 2.95 1.82 3.43 -1.44 -3.35 3.17 0.35 3.57 -2.57
Eisosomes 2.85 2.82 1.35 -6.97 -7.53 -4.65 -20.86 -21.33 -12.00 -1.91 -8.47 -20.26 -23.60 -12.86 -6.75
Endoplasmic Reticulum 3.03 3.06 2.40 -7.64 -8.13 -5.50 -15.55 -16.81 -12.87 -5.42 -5.85 -10.34 -17.82 -14.20 -8.95
Endosome 1.45 2.08 1.41 -0.07 -2.65 -2.81 -4.46 -4.70 2.15 -1.40 -4.02 -1.18 -3.14 1.61 -2.86
Golgi 0.26 1.81 1.00 1.09 -2.24 -1.72 0.08 -1.04 -0.69 -1.05 -2.74 0.31 -1.55 0.03 -1.62
Lipid Particles 2.48 2.81 2.70 3.27 -5.42 -2.81 0.71 -1.54 1.43 -1.70 -3.00 2.69 -0.05 3.20 -3.57
Mitochondria 1.37 2.17 0.01 -2.22 -3.98 -2.96 -7.58 -4.51 -0.99 -1.93 -4.15 -7.59 -6.51 -2.13 -4.21
Mitotic Spindle 1.95 1.85 1.01 -5.14 -4.53 -2.56 -3.81 -3.84 -2.02 -0.72 -1.12 -2.98 -5.82 -5.33 -4.08
None -9.26 -0.99 -2.90 8.25 -2.49 1.97 1.82 -0.29 -2.03 -1.91 -7.64 1.47 -1.15 7.06 -2.86
Nuclear Periphery 1.25 1.31 0.97 -5.82 -8.14 -5.43 -7.55 -7.29 -0.40 1.44 -2.54 -2.45 -5.97 -4.82 -5.92
Nuclear Periphery Foci 2.43 2.47 2.45 1.83 -4.22 -0.57 -1.04 -2.03 -1.55 -1.29 1.55 2.21 1.59 0.20 -3.13
Nucleolus 1.92 2.88 2.81 5.93 -0.74 -0.65 -0.10 -1.64 -1.02 -1.68 -4.49 -0.35 -1.38 3.72 -1.41
Nucleus -2.26 -3.50 -2.22 0.21 8.89 3.75 10.98 11.23 7.26 2.30 9.99 9.09 14.03 4.44 8.03
Peroxisomes 1.08 2.51 2.37 2.32 -1.88 -2.44 -0.61 -2.43 2.15 -2.19 -3.41 0.01 -2.35 3.08 -2.34
Vacuole 2.25 2.26 0.74 -6.59 -6.50 -3.19 -4.64 -5.12 -3.39 -3.20 -3.13 -3.45 -8.37 -6.87 -6.68
Vacuole Periphery 2.11 2.45 1.80 -0.61 -1.57 -2.76 -2.95 -2.88 0.10 -2.07 -2.28 0.12 -1.87 -0.51 -1.91
Standard name
Human Ortholog
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functionally complemented by human NFYA
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hap2

Hap2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hap2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available