Standard name
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functionally complemented by human NFYA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.08 0.05 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0 0.06 0.05 0 0 0 0.12 0.16 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.28 0 0 0 0.1 0.27 0.29 0.29 0.23 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.87 0.94 0.98 0.95 0.89 0.87 0.79 0.78 0.79 0.88 0.83 0.77 0.88 0.84 0.84 0.87 0.82 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.1 0.12 0.26 0.23 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0.09 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud 0 0 0 1 0 0 0 1 1 0 0 0 0 2 0 1 2 0 3
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 1 0 0 2 1 5 2 8 5 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 1 4 4 1 1 11 5 5 1 3 42 64 88 1 4 0 1 5 5
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1
Endosome 0 1 0 0 1 0 0 0 0 0 0 2 4 1 1 0 0 3 6
Golgi 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0
Mitochondria 20 47 1 0 2 18 29 34 30 25 2 0 1 2 3 0 2 1 7
Nucleus 97 144 72 83 122 157 94 92 81 84 309 341 336 105 148 72 185 279 267
Nuclear Periphery 0 3 0 0 1 3 2 1 0 2 0 0 0 0 0 0 0 1 1
Nucleolus 2 3 0 1 3 5 11 14 27 25 0 0 4 0 0 1 4 9 20
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 2 0 0 1 0 1 3 4 0 0 1 1 3 5
Vac/Vac Membrane 0 3 1 0 1 2 0 0 1 0 1 3 7 4 13 3 11 31 55
Unique Cell Count 105 166 77 85 128 177 108 117 104 107 353 410 436 120 176 86 212 342 387
Labelled Cell Count 120 208 79 86 134 199 146 149 150 144 357 416 448 120 176 86 212 342 387


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.1 6.0 6.3 5.9 5.5 5.3 4.9 5.1 5.3 6.5 6.9 6.9 6.9 7.2 7.4
Std Deviation (1e-4) 0.7 5.4 1.1 0.8 1.1 1.4 1.2 1.0 1.4 1.5 1.3 1.5 2.2 1.4 1.7 1.8
Intensity Change (Log2) 0.08 -0.03 -0.13 -0.17 -0.3 -0.24 -0.19 0.12 0.2 0.2 0.21 0.26 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 1.7 2.4 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.5 4.6 0 4.9 4.2 0 0 0
Nucleus 1.3 0.6 -1.2 -1.4 -2.8 -2.9 -2.8 -1.5 -2.3 -3.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 4.8 4.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hap2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available