Standard name
Description Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0.05 0.07 0.06 0 0 0 0.10 0.14 0.14 0.08 0.07 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.16 0.09 0.12 0.10 0.56 0.23 0.37 0.34 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.95 0.92 0.94 0.93 0.87 0.89 0.87 0.82 0.72 0.83 0.80 0.82 0.88 0.85 0.83 0.89 0.85 0.84 0.85 0.86 0.85 0.69 0.60 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.06 0.13 0.19 0.20
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0
Bud 0 0 0 0 5 0 0 6 9 9 6 0 0 0 2 0 0 1 4 6 3 3 0 3
Bud Neck 0 0 1 0 1 0 0 0 2 1 1 0 1 0 0 0 0 0 0 0 0 0 1 3
Bud Site 1 0 0 2 1 6 3 4 22 20 23 25 0 0 0 0 2 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 25 19 20 27 25 34 56 41 19 16 12 51 71 71 28 27 28 3 4 1 6 6 5
Endoplasmic Reticulum 1 2 0 0 0 0 0 1 0 0 0 0 0 0 1 5 8 9 0 0 1 1 2 0
Endosome 1 0 0 0 0 5 4 6 0 2 1 0 3 0 1 0 3 1 1 5 4 7 6 9
Golgi 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 7 0 2 1 0
Mitochondria 12 28 1 38 71 62 86 73 385 161 206 199 0 1 1 18 8 3 0 6 4 7 11 12
Nucleus 271 588 430 427 380 587 630 616 495 591 444 489 446 442 429 319 311 224 256 575 402 285 215 247
Nuclear Periphery 0 5 0 0 0 6 10 7 9 17 6 4 2 5 1 12 10 8 0 0 1 3 4 13
Nucleolus 7 6 1 2 4 2 7 19 31 25 28 13 1 4 5 8 6 5 13 18 16 29 29 52
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0
SpindlePole 0 1 4 2 4 3 3 10 6 5 5 2 1 3 6 2 3 3 3 2 4 5 6 8
Vac/Vac Membrane 6 16 5 7 8 18 16 29 12 16 9 12 5 4 7 7 18 11 12 34 28 55 66 93
Unique Cell Count 286 642 457 460 436 658 728 748 688 709 555 594 504 519 517 358 365 266 300 671 476 413 358 458
Labelled Cell Count 304 671 462 499 501 715 793 827 1012 866 745 756 511 530 525 399 396 294 300 671 476 413 358 458

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.7 6.7 5.8 5.2 5.4 5.3 5.6 4.2 5.0 4.6 4.8 6.7 6.5 6.2 6.7 8.1 8.2 6.9 7.4 7.2
Std Deviation (1e-4) 1.2 1.0 1.3 1.2 1.4 1.3 1.2 1.4 1.1 0.9 1.0 0.8 1.5 1.3 1.3 1.4 2.0 2.3 1.5 1.5 1.5
Intensity Change (Log2) -0.21 -0.35 -0.3 -0.33 -0.25 -0.66 -0.43 -0.54 -0.49 0.0 -0.04 -0.1 -0.0 0.28 0.29 0.06 0.14 0.11

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.6 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -1.9 -2.6 -2.2 -1.7 0 -4.2 0 0 -1.7 -0.7 -1.5 0.1 0.8 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.0 8.8 6.5 7.4 6.7 0 10.8 0 0 0 0 0 4.5 0 0
Nucleus 0 0 0 0 0 0 3.6 0 0 0 0 0 0 4.2 0
Nuclear Periphery 0 0 0 0 0 0 3.3 0 0 0 0 0 3.9 0 0
Nucleolus 0 0 0 0 3.1 0 3.7 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.7 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.0 2.1 1.6 2.6 0 1.0 0 0 0 0 0.7 0 2.9 0