Standard name
Human Ortholog
Description Zeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0
Cytoplasm 0.93 0.95 0.95 0.92 0.9 0.75 0.7 0.65 0.65 0.67 0.58 0.63 0.9 0.97 0.98 0.92 0.9 0.88 0.61 0.71 0.61 0.58 0.64 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0.05 0.08 0.07 0.15 0.07 0.14 0.09 0.06 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.32 0.45 0.48 0.51 0.41 0.58 0.53 0 0 0 0 0 0 0.07 0.06 0.1 0.14 0.1 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 0 0 1 1 0 0 0 0 0 0 2 0 0 4 3 3 27 12 12 5 4 10
Bud 1 3 2 0 1 3 10 14 14 4 20 11 0 0 0 2 1 0 28 15 6 9 10 15
Bud Neck 6 3 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 3 10 9 4 5 5 5
Bud Site 0 0 0 0 0 0 0 1 6 2 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 2 4 5 2 1 2 0 4 3 3 3 6 3 7 19 7 22 16 7 10 4 4 4
Cytoplasm 806 442 372 275 384 350 415 402 370 307 388 350 445 386 594 314 202 248 562 354 246 206 209 228
Endoplasmic Reticulum 17 10 9 1 3 18 4 1 3 2 8 7 50 7 5 17 17 19 142 33 56 32 18 25
Endosome 6 0 2 2 8 2 2 2 4 10 1 2 1 1 1 0 2 1 15 7 6 2 6 4
Golgi 3 2 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 1 2 3 2 2 1 5
Mitochondria 9 4 13 13 38 152 263 294 289 187 393 299 1 0 0 6 5 6 62 31 41 50 32 44
Nucleus 1 0 0 1 3 2 4 8 0 1 5 3 1 1 1 2 1 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 1 1 3 3 0 1 1 0 0 0 0 0 0 0 2 0 1 0 3 0
Nucleolus 0 0 1 0 1 2 1 2 3 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 1 0 0 0 3 0 1 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 1 2 3 0 2 6 2 6 4 3 9 0 0 1 0 0 0 13 5 1 2 3 3
Vac/Vac Membrane 27 5 3 11 16 6 13 11 21 23 11 10 14 6 8 8 8 14 20 13 5 23 16 20
Unique Cell Count 864 465 392 298 427 469 589 616 572 457 672 559 496 396 608 342 224 283 928 503 403 356 329 374
Labelled Cell Count 883 474 408 313 458 541 723 740 720 547 838 696 521 406 619 372 247 318 928 503 403 356 329 374


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.3 4.1 4.3 4.1 3.9 3.2 3.5 3.4 3.4 3.2 3.3 5.8 5.6 5.5 6.1 5.9 6.3 6.0 6.2 5.8
Std Deviation (1e-4) 0.9 0.9 0.9 1.5 1.5 1.2 0.9 1.3 1.1 1.0 0.8 0.7 1.3 1.4 1.4 1.0 1.2 1.2 1.8 2.2 1.8
Intensity Change (Log2) – – – 0.08 0.02 -0.07 -0.34 -0.22 -0.27 -0.25 -0.33 -0.31 0.51 0.46 0.44 0.58 0.54 0.64 0.57 0.6 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 1.8 – 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1137 0.7678 0.5463 0.3929 0.324 0.7196 2.3179 3.2555 2.8978 3.1483 2.7004 3.1808 2.416 1.2832 1.3303 1.9245 2.6851 1.2405
Actin 0.0007 0.0038 0.0018 0.0035 0.0002 0.0021 0.0088 0.0005 0.0003 0.0019 0.0007 0.0009 0.0393 0.0005 0.0122 0.0272 0.002 0.0007
Bud 0.0018 0.0033 0.0015 0.0001 0 0.0041 0.0001 0.0003 0.0004 0.0003 0 0.0001 0.0011 0.0024 0.0001 0.0004 0.0018 0.0001
Bud Neck 0.0006 0.0016 0.0006 0.0004 0.0001 0.0042 0.0011 0.0002 0.0003 0.0009 0.0002 0.0011 0.0067 0.0001 0.0001 0.0012 0.0002 0.0005
Bud Periphery 0.0012 0.0011 0.0007 0.0002 0 0.0014 0.0001 0.0003 0.0005 0.0004 0 0.0002 0.0017 0.0024 0.0001 0.0007 0.0041 0.0001
Bud Site 0.002 0.0041 0.0062 0.0008 0.0001 0.0011 0.0006 0.0081 0.0021 0.0002 0 0.0006 0.0083 0.0051 0.0003 0.0017 0.0005 0.0002
Cell Periphery 0.0003 0.0004 0.0002 0.0004 0.0001 0.0002 0.0005 0.0006 0.0006 0.0012 0.0001 0.0002 0.0008 0.0005 0.0003 0.0002 0.0003 0.0002
Cytoplasm 0.1162 0.137 0.1321 0.1469 0.0574 0.1278 0.1897 0.2635 0.2756 0.4378 0.2734 0.2502 0.1286 0.3007 0.2931 0.2138 0.2429 0.3801
Cytoplasmic Foci 0.0124 0.0072 0.0046 0.0132 0.0022 0.0073 0.0092 0.0064 0.0073 0.0096 0.0578 0.0066 0.0402 0.0055 0.0206 0.0083 0.0023 0.006
Eisosomes 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0015 0.0001 0.0001 0.0002 0.0001 0.0001 0.001 0.0001 0.0001 0.0007 0.0001 0.0001
Endoplasmic Reticulum 0.0092 0.0115 0.007 0.0202 0.0008 0.008 0.0271 0.0398 0.042 0.089 0.0522 0.0194 0.0351 0.0331 0.0306 0.0858 0.0092 0.0414
Endosome 0.0022 0.0031 0.0023 0.0053 0.0009 0.0011 0.0052 0.0057 0.0101 0.0183 0.0036 0.0044 0.0333 0.0038 0.0134 0.0313 0.0071 0.0056
Golgi 0.0002 0.0004 0.0003 0.0008 0 0.0001 0.0009 0.0003 0.0003 0.0006 0.0003 0.0002 0.0082 0.0003 0.0047 0.0101 0.0003 0.0006
Lipid Particles 0.0007 0.0007 0.0005 0.0021 0.0002 0.0003 0.0018 0.0007 0.0014 0.0027 0.0009 0.0012 0.0196 0.0008 0.0041 0.013 0.0003 0.0008
Mitochondria 0.0004 0.0009 0.0003 0.0006 0.0002 0.001 0.0003 0.0003 0.0004 0.0016 0.0004 0.0002 0.0228 0.0004 0.0016 0.0035 0.003 0.0004
None 0.8421 0.8086 0.8184 0.792 0.932 0.8232 0.7458 0.6627 0.6453 0.3987 0.5997 0.7074 0.5744 0.6362 0.5997 0.5428 0.5894 0.5477
Nuclear Periphery 0.0016 0.0021 0.0022 0.0021 0.0014 0.001 0.0018 0.0029 0.0034 0.016 0.0021 0.0021 0.0272 0.0023 0.006 0.0277 0.0513 0.0041
Nucleolus 0.0008 0.0012 0.001 0.0004 0.0003 0.0019 0.0002 0.0003 0.0005 0.0005 0.0001 0.0001 0.0042 0.0003 0.0002 0.0014 0.001 0.0003
Nucleus 0.0027 0.0062 0.0086 0.0027 0.002 0.0069 0.0016 0.003 0.0029 0.0092 0.0007 0.0016 0.0076 0.002 0.0013 0.0072 0.0627 0.0028
Peroxisomes 0.0001 0.0003 0.0002 0.0023 0.0001 0.0003 0.0003 0.0002 0.0001 0.0004 0.0006 0.0001 0.0113 0.0001 0.0019 0.006 0.0001 0.0003
Punctate Nuclear 0.0033 0.0048 0.0102 0.0047 0.0014 0.0068 0.0011 0.0012 0.0016 0.0042 0.0057 0.0017 0.0219 0.001 0.0071 0.0099 0.0024 0.0064
Vacuole 0.0012 0.0013 0.0008 0.0011 0.0005 0.0008 0.0021 0.0027 0.0045 0.0053 0.0013 0.0012 0.0045 0.0024 0.0018 0.0052 0.0103 0.0017
Vacuole Periphery 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0003 0.0011 0.0002 0.0001 0.0023 0.0002 0.0005 0.0015 0.0087 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.1453 83.1428 64.4999 87.167 87.7004 54.9284 95.5291 73.5023 82.1201 99.3441
Translational Efficiency 1.521 1.8119 2.041 1.4077 1.7591 3.5951 1.8592 1.9649 1.7745 2.0556

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1070 1572 156 1910 1956 1532 1185 81 3026 3104 1341 1991

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 665.27 722.01 860.15 796.65 685.12 702.15 824.92 858.93 678.10 712.21 829.02 799.18
Standard Deviation 80.56 99.88 87.23 107.95 81.78 99.85 104.10 113.26 81.90 100.36 102.90 108.87
Intensity Change Log 2 — 0.118079 0.370648 0.260006 — 0.035423 0.267898 0.326184 — 0.076735 0.319432 0.293961

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000078 0.000369 0.000363 0.001359 0.000055 0.000597 0.000429 0.000178 0.000063 0.000481 0.000421 0.001311
Bud Neck 0.005787 0.016387 0.001658 0.009509 0.009810 0.027889 0.004890 0.000937 0.008387 0.022064 0.004514 0.009161
Bud Site 0.004273 0.014805 0.003929 0.026196 0.001967 0.012190 0.013128 0.001557 0.002783 0.013514 0.012058 0.025193
Cell Periphery 0.000365 0.000872 0.000419 0.000320 0.000138 0.000394 0.000331 0.000798 0.000218 0.000636 0.000341 0.000340
Cytoplasm 0.562874* 0.493397* 0.285062* 0.475971* 0.539836* 0.435172* 0.469345* 0.409331* 0.547982* 0.464660* 0.447907* 0.473260*
Cytoplasmic Foci 0.190739* 0.192507* 0.080217 0.027271 0.177304 0.236877* 0.034478 0.124757 0.182055* 0.214406* 0.039799 0.031237
Eisosomes 0.000136 0.000203 0.000176 0.000074 0.000146 0.000423 0.000084 0.000238 0.000142 0.000312 0.000094 0.000081
Endoplasmic Reticulum 0.000554 0.005434 0.025458 0.015040 0.002280 0.011050 0.039501 0.052876 0.001670 0.008206 0.037868 0.016579
Endosome 0.007511 0.012790 0.013308 0.004386 0.007690 0.022324 0.004218 0.014325 0.007627 0.017496 0.005275 0.004790
Golgi 0.000843 0.002103 0.000176 0.001272 0.000920 0.006094 0.000140 0.000712 0.000893 0.004073 0.000144 0.001249
Lipid Particles 0.007130 0.012342 0.014218 0.004122 0.009655 0.027019 0.006688 0.017933 0.008762 0.019586 0.007564 0.004684
Mitochondria 0.001161 0.003092 0.000283 0.001231 0.003640 0.008342 0.000365 0.000227 0.002763 0.005683 0.000356 0.001190
Mitotic Spindle 0.000568 0.002891 0.000108 0.010260 0.001853 0.002836 0.003213 0.000021 0.001398 0.002864 0.002852 0.009843
None 0.006374 0.005625 0.001822 0.003542 0.003903 0.002429 0.002084 0.003306 0.004777 0.004048 0.002053 0.003532
Nuclear Periphery 0.000373 0.000831 0.011904 0.004232 0.001000 0.001740 0.009371 0.004431 0.000778 0.001280 0.009665 0.004240
Nuclear Periphery Foci 0.000631 0.001503 0.033784 0.005539 0.000691 0.002299 0.015233 0.018953 0.000670 0.001896 0.017391 0.006084
Nucleolus 0.001058 0.001296 0.001492 0.000586 0.001109 0.001457 0.000369 0.001178 0.001091 0.001375 0.000500 0.000610
Nucleus 0.174855* 0.159353* 0.390962* 0.295565* 0.187955* 0.118183 0.319849* 0.242115* 0.183323* 0.139033* 0.328121* 0.293391*
Peroxisomes 0.001993 0.007188 0.000938 0.000564 0.002644 0.012565 0.000500 0.000878 0.002414 0.009842 0.000551 0.000577
Vacuole 0.031943 0.065887 0.133372 0.112617 0.046766 0.068835 0.075595 0.104964 0.041524 0.067342 0.082317 0.112306
Vacuole Periphery 0.000754 0.001124 0.000351 0.000344 0.000638 0.001283 0.000189 0.000287 0.000679 0.001203 0.000208 0.000342

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.17 -5.21 -2.63 -1.79 -1.72 -3.69 -1.98 -3.78 2.74 1.25 -5.20 -2.18 -2.69 -1.43 -1.52
Bud Neck -7.03 5.66 -2.84 4.80 -8.55 -8.01 4.06 9.95 12.99 5.41 -9.89 4.05 -0.15 9.26 -3.75
Bud Site -5.14 0.27 -7.33 -2.14 -6.06 -6.75 -4.15 0.80 7.08 4.32 -8.58 -3.81 -8.48 -2.82 -3.08
Cell Periphery -2.65 -0.53 1.15 3.56 4.09 -3.59 -7.63 -4.88 -2.55 -3.41 -4.26 -3.03 -1.85 3.69 1.65
Cytoplasm 7.28 15.03 13.12 5.78 -8.69 11.66 8.86 5.29 1.47 2.12 12.75 13.33 14.63 3.42 -0.46
Cytoplasmic Foci -0.16 11.54 29.52 33.69 6.26 -8.81 33.01 3.85 7.80 -6.70 -6.48 38.59 43.95 48.56 4.26
Eisosomes -4.28 -3.59 9.41 8.63 8.25 -8.22 6.89 -3.06 3.93 -4.97 -9.07 6.46 9.76 12.57 2.89
Endoplasmic Reticulum -5.18 -4.23 -9.52 -4.28 2.02 -5.41 -9.45 -2.92 -2.35 -0.76 -6.89 -10.24 -8.71 -3.22 6.10
Endosome -4.92 -3.68 5.34 10.33 6.50 -9.03 6.54 -3.10 2.99 -4.85 -10.25 4.98 7.39 14.64 2.71
Golgi -4.05 3.52 0.01 2.26 -1.35 -7.21 6.73 0.97 7.22 -1.99 -8.23 7.45 0.14 5.35 -1.46
Lipid Particles -5.05 -3.77 5.43 10.50 6.14 -9.55 4.76 -2.77 3.10 -4.03 -10.34 2.61 9.58 15.65 6.11
Mitochondria -3.34 2.17 0.28 4.47 -3.56 -3.35 4.18 4.37 7.04 3.23 -3.60 4.56 3.14 7.32 -3.14
Mitotic Spindle -2.17 1.05 -6.29 -4.03 -6.64 -1.05 -1.59 2.46 3.91 3.38 -1.90 -1.92 -5.52 -4.36 -3.43
None 0.63 4.90 3.12 2.94 -2.48 1.96 2.96 0.51 -0.51 -0.85 1.07 5.31 2.27 1.42 -2.44
Nuclear Periphery -5.61 -3.36 -12.14 -10.41 2.04 -2.79 -6.32 -1.75 -1.33 2.13 -3.42 -7.19 -10.68 -8.77 4.02
Nuclear Periphery Foci -5.26 -4.25 -8.42 -6.47 3.59 -3.68 -9.16 -3.92 -3.53 -0.74 -5.23 -10.02 -9.08 -6.34 6.45
Nucleolus -1.43 -1.46 3.68 5.49 3.87 -1.00 3.67 -0.18 0.64 -2.46 -1.37 4.10 3.75 5.10 -0.46
Nucleus 2.52 -9.55 -15.43 -18.05 4.53 13.79 -17.08 -2.72 -5.56 2.84 11.13 -20.15 -16.36 -23.76 5.59
Peroxisomes -6.64 3.43 5.12 9.39 4.47 -8.74 6.98 5.75 10.68 -2.46 -10.74 7.87 8.90 14.40 0.36
Vacuole -12.02 -9.21 -26.71 -18.76 -1.20 -7.42 -10.26 -4.73 -3.18 -1.58 -12.44 -14.03 -25.63 -19.77 -11.54
Vacuole Periphery -0.96 1.44 1.69 4.35 1.44 -3.97 4.59 3.51 7.08 -2.03 -3.06 3.87 3.28 7.54 -1.41
Standard name
Human Ortholog
Description Zeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins
Localization
Cell Percentages cytoplasm (42%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ost5

Ost5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ost5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available