Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 5 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 7 | 1 | 0 | 0 | 0 | 1 | 4 | 7 | 6 | 24 | 37 |
Bud | 0 | 3 | 21 | 21 | 8 | 12 | 29 | 26 | 17 | 35 | 16 | 29 | 5 | 14 | 5 | 0 | 2 | 0 | 0 | 2 | 2 | 3 | 5 | 5 |
Bud Neck | 0 | 0 | 5 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 3 | 7 | 14 |
Bud Site | 0 | 0 | 3 | 2 | 1 | 4 | 7 | 7 | 9 | 10 | 3 | 10 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 10 | 4 | 3 | 3 | 2 | 2 | 0 | 3 | 3 | 0 | 1 | 22 | 15 | 14 | 14 | 14 | 18 | 0 | 0 | 1 | 0 | 2 | 0 |
Cytoplasm | 13 | 23 | 115 | 40 | 36 | 27 | 52 | 57 | 41 | 49 | 45 | 58 | 57 | 113 | 144 | 40 | 47 | 47 | 4 | 1 | 10 | 3 | 3 | 1 |
Endoplasmic Reticulum | 7 | 13 | 3 | 1 | 3 | 1 | 1 | 0 | 2 | 6 | 2 | 1 | 18 | 12 | 10 | 17 | 10 | 6 | 0 | 2 | 12 | 1 | 7 | 5 |
Endosome | 0 | 18 | 3 | 0 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 88 | 71 | 95 | 31 | 34 | 20 | 5 | 30 | 23 | 28 | 55 | 53 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 11 | 2 | 1 | 1 | 4 | 0 | 7 | 3 | 21 | 36 | 25 |
Mitochondria | 87 | 141 | 221 | 224 | 267 | 257 | 440 | 375 | 421 | 462 | 326 | 421 | 262 | 197 | 174 | 9 | 15 | 14 | 80 | 193 | 275 | 287 | 452 | 377 |
Nucleus | 2 | 2 | 3 | 0 | 1 | 9 | 8 | 10 | 6 | 4 | 2 | 1 | 5 | 3 | 3 | 7 | 9 | 8 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 1 | 0 | 8 | 2 | 6 | 2 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 5 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 5 | 1 | 1 | 2 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 2 |
Peroxisomes | 0 | 3 | 3 | 3 | 6 | 0 | 3 | 1 | 1 | 1 | 1 | 2 | 34 | 23 | 43 | 18 | 16 | 12 | 0 | 5 | 12 | 16 | 32 | 20 |
SpindlePole | 0 | 6 | 23 | 9 | 9 | 3 | 21 | 22 | 10 | 13 | 10 | 27 | 26 | 13 | 23 | 29 | 26 | 39 | 0 | 3 | 4 | 6 | 5 | 7 |
Vac/Vac Membrane | 9 | 23 | 9 | 7 | 21 | 12 | 13 | 20 | 12 | 12 | 3 | 8 | 87 | 57 | 79 | 41 | 33 | 22 | 11 | 12 | 14 | 19 | 29 | 26 |
Unique Cell Count | 100 | 208 | 344 | 247 | 313 | 276 | 483 | 432 | 448 | 499 | 354 | 458 | 515 | 457 | 516 | 167 | 163 | 151 | 113 | 275 | 384 | 411 | 677 | 588 |
Labelled Cell Count | 124 | 250 | 413 | 310 | 365 | 331 | 590 | 523 | 533 | 599 | 409 | 563 | 627 | 538 | 595 | 207 | 209 | 192 | 113 | 275 | 384 | 411 | 677 | 588 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 5.3 | 3.3 | 3.2 | 3.9 | 3.3 | 3.2 | 3.3 | 2.8 | 3.0 | 3.0 | 2.9 | 5.5 | 5.4 | 5.3 | 10.8 | 7.1 | 7.4 | 5.6 | 5.4 | 5.5 |
Std Deviation (1e-4) | 0.5 | 0.9 | 0.8 | 1.1 | 1.3 | 1.4 | 1.4 | 1.3 | 0.8 | 1.0 | 1.2 | 0.8 | 1.1 | 1.1 | 1.1 | 9.3 | 2.0 | 2.6 | 1.7 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.24 | -0.03 | -0.07 | 0.01 | -0.27 | -0.17 | -0.14 | -0.21 | 0.72 | 0.71 | 0.67 | 1.7 | 1.1 | 1.15 | 0.76 | 0.71 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.3 | -2.1 | -4.3 | -3.3 | -2.5 | -3.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.0 | 1.6 | 4.2 | 4.2 | 5.3 |
Cytoplasm | -4.7 | -6.7 | -7.0 | -8.0 | -6.7 | -8.5 | -8.5 | -6.5 | -7.1 | -8.0 | -2.7 | -1.7 | -2.2 | -1.0 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 1.8 | 1.3 | 5.1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.6 | 7.1 | 7.9 | 7.5 | 8.1 | 6.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.9 | -5.9 | -8.8 | -12.5 | -11.6 | -11.3 |
Nucleus | 0 | 0 | 2.1 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 3.3 | 4.8 | 5.3 | 5.0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -2.6 | -1.4 | 3.7 | 3.3 | 5.9 |
Vacuole | 0.2 | 2.5 | 1.2 | 0.1 | 1.5 | 0.1 | -0.2 | -1.8 | -0.8 | 6.5 | 5.0 | 6.0 | 7.8 | 6.7 | 5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0836 | 1.7775 | 1.4008 | 1.1756 | 1.6672 | 1.4527 | 0.4828 | 0.5769 | 0.621 | 1.3328 | 0.6436 | 0.7128 | 1.8838 | 2.0028 | 1.9538 | 2.1469 | 1.0469 | 2.2768 |
Actin | 0.0127 | 0.0147 | 0.007 | 0.0021 | 0.0143 | 0.0131 | 0.0192 | 0.0077 | 0.0193 | 0.0503 | 0.0082 | 0.0138 | 0.0592 | 0.0182 | 0.0113 | 0.0637 | 0.1473 | 0.0291 |
Bud | 0.0008 | 0.0049 | 0.0036 | 0.0032 | 0.0097 | 0.0011 | 0.0032 | 0.0048 | 0.0041 | 0.0015 | 0.0212 | 0.0011 | 0.0018 | 0.0031 | 0.0085 | 0.0022 | 0.0058 | 0.0018 |
Bud Neck | 0.0076 | 0.0023 | 0.0087 | 0.0127 | 0.0013 | 0.0057 | 0.002 | 0.0015 | 0.008 | 0.0015 | 0.0023 | 0.005 | 0.0049 | 0.0018 | 0.0016 | 0.0009 | 0.003 | 0.0088 |
Bud Periphery | 0.0018 | 0.0075 | 0.0138 | 0.015 | 0.0239 | 0.0029 | 0.0065 | 0.0108 | 0.0099 | 0.004 | 0.0265 | 0.0042 | 0.0048 | 0.0062 | 0.008 | 0.0057 | 0.0168 | 0.0053 |
Bud Site | 0.0065 | 0.0236 | 0.0076 | 0.0038 | 0.0045 | 0.0021 | 0.0108 | 0.0329 | 0.031 | 0.0029 | 0.006 | 0.0014 | 0.0049 | 0.033 | 0.0159 | 0.0021 | 0.0078 | 0.002 |
Cell Periphery | 0.0014 | 0.0018 | 0.0029 | 0.0044 | 0.0005 | 0.0008 | 0.0019 | 0.0025 | 0.0022 | 0.0006 | 0.001 | 0.0008 | 0.0023 | 0.0019 | 0.0018 | 0.0008 | 0.0025 | 0.0016 |
Cytoplasm | 0.0128 | 0.0413 | 0.0646 | 0.036 | 0.0272 | 0.0289 | 0.0231 | 0.0578 | 0.0364 | 0.0055 | 0.0184 | 0.0236 | 0.023 | 0.0377 | 0.0412 | 0.0118 | 0.0198 | 0.0521 |
Cytoplasmic Foci | 0.0744 | 0.0984 | 0.0617 | 0.0245 | 0.0624 | 0.0691 | 0.0738 | 0.0965 | 0.0773 | 0.0829 | 0.0851 | 0.0586 | 0.0866 | 0.0943 | 0.1049 | 0.0316 | 0.0543 | 0.1026 |
Eisosomes | 0.0004 | 0.0008 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0011 | 0.0005 | 0.0009 | 0.0003 | 0.0002 | 0.0004 | 0.0025 | 0.0012 | 0.0018 | 0.0007 | 0.0135 | 0.0009 |
Endoplasmic Reticulum | 0.0032 | 0.0054 | 0.0037 | 0.0026 | 0.0017 | 0.0034 | 0.005 | 0.0049 | 0.0025 | 0.0009 | 0.0033 | 0.0019 | 0.0171 | 0.0057 | 0.0023 | 0.0035 | 0.0023 | 0.0052 |
Endosome | 0.1828 | 0.1573 | 0.16 | 0.1668 | 0.1746 | 0.1427 | 0.1607 | 0.1315 | 0.123 | 0.1089 | 0.1195 | 0.0935 | 0.159 | 0.1784 | 0.1291 | 0.0521 | 0.0962 | 0.1489 |
Golgi | 0.075 | 0.0919 | 0.0776 | 0.0627 | 0.0576 | 0.0774 | 0.0534 | 0.0625 | 0.054 | 0.0902 | 0.0612 | 0.0531 | 0.0816 | 0.0908 | 0.0723 | 0.0278 | 0.0821 | 0.0963 |
Lipid Particles | 0.034 | 0.0334 | 0.0282 | 0.0044 | 0.0535 | 0.0363 | 0.0426 | 0.0141 | 0.0172 | 0.0388 | 0.0312 | 0.0205 | 0.0403 | 0.0324 | 0.023 | 0.0109 | 0.0095 | 0.0469 |
Mitochondria | 0.48 | 0.3869 | 0.4944 | 0.5727 | 0.5021 | 0.5346 | 0.4403 | 0.4723 | 0.4986 | 0.5492 | 0.4985 | 0.6119 | 0.3736 | 0.3877 | 0.4723 | 0.7043 | 0.4821 | 0.3904 |
None | 0.0361 | 0.0518 | 0.0173 | 0.0082 | 0.004 | 0.0236 | 0.0652 | 0.0275 | 0.044 | 0.0101 | 0.0282 | 0.0293 | 0.0669 | 0.0485 | 0.0273 | 0.0379 | 0.0293 | 0.041 |
Nuclear Periphery | 0.0024 | 0.0017 | 0.0026 | 0.0012 | 0.0094 | 0.0023 | 0.0088 | 0.0027 | 0.0012 | 0.0005 | 0.0105 | 0.005 | 0.0093 | 0.0013 | 0.0005 | 0.0016 | 0.0003 | 0.0018 |
Nucleolus | 0.0013 | 0.0018 | 0.0008 | 0.0005 | 0.002 | 0.001 | 0.004 | 0.0014 | 0.0016 | 0.0012 | 0.0023 | 0.0006 | 0.0007 | 0.0011 | 0.0005 | 0.0005 | 0.0004 | 0.0005 |
Nucleus | 0.0023 | 0.0019 | 0.0014 | 0.0013 | 0.0034 | 0.0019 | 0.0063 | 0.0031 | 0.0018 | 0.0008 | 0.0059 | 0.0013 | 0.0032 | 0.0015 | 0.0007 | 0.0008 | 0.0004 | 0.0008 |
Peroxisomes | 0.024 | 0.036 | 0.0207 | 0.0083 | 0.019 | 0.0334 | 0.0394 | 0.0435 | 0.0458 | 0.0375 | 0.0455 | 0.0634 | 0.0383 | 0.0334 | 0.0616 | 0.0274 | 0.0192 | 0.0429 |
Punctate Nuclear | 0.002 | 0.004 | 0.0008 | 0.0002 | 0.0126 | 0.0039 | 0.0063 | 0.0011 | 0.0007 | 0.0024 | 0.0073 | 0.0009 | 0.001 | 0.0054 | 0.0005 | 0.0004 | 0.0005 | 0.0007 |
Vacuole | 0.0263 | 0.0225 | 0.0137 | 0.0392 | 0.0088 | 0.0093 | 0.0188 | 0.0154 | 0.014 | 0.006 | 0.0109 | 0.006 | 0.0142 | 0.0113 | 0.01 | 0.0081 | 0.0039 | 0.0142 |
Vacuole Periphery | 0.012 | 0.0101 | 0.0084 | 0.0302 | 0.0072 | 0.006 | 0.0075 | 0.0051 | 0.0065 | 0.0042 | 0.0067 | 0.0037 | 0.0049 | 0.005 | 0.0051 | 0.005 | 0.003 | 0.0061 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.2122 | 10.6062 | 9.8378 | 14.923 | 24.9973 | 11.858 | 14.7454 | 16.2853 | 22.6688 | 17.5041 |
Translational Efficiency | 1.6937 | 3.2058 | 2.6192 | 2.1067 | 1.4611 | 4.9863 | 2.3244 | 1.5751 | 1.7841 | 2.0181 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1531 | 1245 | 2041 | 1191 | 1464 | 1946 | 1585 | 158 | 2995 | 3191 | 3626 | 1349 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 659.61 | 754.34 | 829.54 | 817.54 | 583.32 | 749.27 | 766.47 | 835.27 | 622.32 | 751.25 | 801.97 | 819.62 |
Standard Deviation | 123.71 | 106.95 | 103.79 | 103.52 | 80.76 | 95.19 | 93.31 | 101.53 | 111.65 | 99.97 | 104.16 | 103.45 |
Intensity Change Log 2 | — | 0.193602 | 0.330698 | 0.309676 | — | 0.361198 | 0.393942 | 0.517955 | — | 0.274685 | 0.360725 | 0.411176 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001331 | 0.002073 | 0.001937 | 0.010682 | 0.000610 | 0.000616 | 0.002810 | 0.004455 | 0.000978 | 0.001184 | 0.002318 | 0.009952 |
Bud Neck | 0.031678 | 0.010545 | 0.042377 | 0.036101 | 0.035730 | 0.011436 | 0.037903 | 0.036445 | 0.033659 | 0.011088 | 0.040421 | 0.036142 |
Bud Site | 0.035778 | 0.021696 | 0.045001 | 0.101884* | 0.015515 | 0.013986 | 0.072518 | 0.078770 | 0.025873 | 0.016995 | 0.057029 | 0.099177 |
Cell Periphery | 0.000486 | 0.000660 | 0.000130 | 0.000262 | 0.000397 | 0.000243 | 0.000241 | 0.000188 | 0.000442 | 0.000406 | 0.000179 | 0.000253 |
Cytoplasm | 0.136280* | 0.017619 | 0.160368* | 0.122445* | 0.033582 | 0.011198 | 0.042293 | 0.070075 | 0.086080 | 0.013703 | 0.108755* | 0.116311* |
Cytoplasmic Foci | 0.166700 | 0.107089 | 0.158588 | 0.160241 | 0.113486 | 0.082504 | 0.079714 | 0.187051* | 0.140688 | 0.092096 | 0.124111 | 0.163381 |
Eisosomes | 0.000246 | 0.000182 | 0.000086 | 0.000194 | 0.000194 | 0.000111 | 0.000115 | 0.000190 | 0.000221 | 0.000139 | 0.000099 | 0.000194 |
Endoplasmic Reticulum | 0.001144 | 0.000542 | 0.006166 | 0.005144 | 0.001400 | 0.000340 | 0.002124 | 0.002857 | 0.001269 | 0.000419 | 0.004399 | 0.004876 |
Endosome | 0.048459 | 0.076098 | 0.045589 | 0.052636 | 0.077348 | 0.084210 | 0.099681 | 0.088255 | 0.062580 | 0.081045 | 0.069233 | 0.056808 |
Golgi | 0.068322 | 0.157023* | 0.019729 | 0.032276 | 0.076756 | 0.163613* | 0.044746 | 0.023157 | 0.072444 | 0.161042* | 0.030664 | 0.031208 |
Lipid Particles | 0.012313 | 0.003600 | 0.006595 | 0.005197 | 0.022247 | 0.004100 | 0.004071 | 0.004949 | 0.017169 | 0.003905 | 0.005491 | 0.005168 |
Mitochondria | 0.405613* | 0.500481* | 0.079514 | 0.079059 | 0.514126* | 0.542724* | 0.303411* | 0.053052 | 0.458656* | 0.526242* | 0.177384* | 0.076013 |
Mitotic Spindle | 0.006933 | 0.015882 | 0.101850* | 0.097062 | 0.006421 | 0.012715 | 0.078583 | 0.052025 | 0.006683 | 0.013951 | 0.091679 | 0.091787 |
None | 0.001759 | 0.000623 | 0.002215 | 0.000804 | 0.004088 | 0.000375 | 0.000796 | 0.000371 | 0.002897 | 0.000472 | 0.001595 | 0.000753 |
Nuclear Periphery | 0.000193 | 0.000170 | 0.003846 | 0.001028 | 0.000423 | 0.000164 | 0.000776 | 0.000469 | 0.000306 | 0.000167 | 0.002504 | 0.000962 |
Nuclear Periphery Foci | 0.000536 | 0.000389 | 0.002054 | 0.001715 | 0.000467 | 0.000131 | 0.000644 | 0.001173 | 0.000502 | 0.000232 | 0.001437 | 0.001652 |
Nucleolus | 0.001055 | 0.001190 | 0.006897 | 0.001958 | 0.003914 | 0.001093 | 0.003619 | 0.001370 | 0.002453 | 0.001131 | 0.005464 | 0.001889 |
Nucleus | 0.008925 | 0.002208 | 0.125537 | 0.035410 | 0.010645 | 0.001637 | 0.015704 | 0.028739 | 0.009766 | 0.001860 | 0.077527 | 0.034629 |
Peroxisomes | 0.036049 | 0.049981 | 0.031523 | 0.106078* | 0.039983 | 0.037503 | 0.052620 | 0.156568* | 0.037972 | 0.042372 | 0.040745 | 0.111992* |
Vacuole | 0.026446 | 0.018429 | 0.146983 | 0.142788 | 0.026479 | 0.017417 | 0.128380 | 0.197285 | 0.026462 | 0.017812 | 0.138852 | 0.149171 |
Vacuole Periphery | 0.009755 | 0.013519 | 0.013017 | 0.007036 | 0.016188 | 0.013883 | 0.029253 | 0.012557 | 0.012900 | 0.013741 | 0.020114 | 0.007682 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.23 | -2.63 | -7.51 | -6.13 | -6.91 | -0.06 | -5.10 | -3.87 | -3.88 | -1.40 | -0.83 | -5.86 | -8.18 | -7.80 | -6.71 |
Bud Neck | 8.09 | -4.03 | -1.32 | -10.54 | 2.80 | 8.15 | -0.82 | 0.15 | -4.51 | 0.68 | 11.40 | -3.35 | -0.74 | -11.77 | 2.39 |
Bud Site | 4.77 | -2.61 | -11.85 | -15.29 | -10.53 | 0.90 | -14.86 | -5.88 | -6.05 | -0.04 | 5.33 | -12.87 | -15.55 | -18.04 | -7.93 |
Cell Periphery | -0.55 | 7.37 | 4.76 | 1.35 | -5.70 | 3.79 | 4.04 | 5.11 | 2.25 | 2.18 | 0.29 | 8.51 | 6.05 | 1.41 | -3.13 |
Cytoplasm | 18.49 | -3.14 | 0.62 | -20.19 | 4.16 | 8.45 | -2.91 | -4.09 | -6.27 | -3.13 | 20.02 | -5.12 | -6.49 | -22.40 | -2.74 |
Cytoplasmic Foci | 10.01 | 2.49 | 2.59 | -7.43 | 0.33 | 7.01 | 7.32 | -4.99 | -7.45 | -7.60 | 13.17 | 5.22 | -2.43 | -13.04 | -6.79 |
Eisosomes | 2.01 | 10.24 | 3.08 | -0.22 | -9.68 | 5.79 | 5.34 | 0.09 | -3.88 | -3.59 | 5.43 | 11.33 | 2.25 | -3.51 | -9.27 |
Endoplasmic Reticulum | 3.66 | -16.06 | -4.99 | -5.76 | 1.38 | 3.40 | -2.27 | -2.85 | -5.63 | -1.49 | 4.89 | -13.20 | -5.05 | -6.33 | -0.52 |
Endosome | -6.57 | 1.62 | 0.44 | 6.97 | -1.15 | -1.57 | -4.32 | -0.15 | 0.62 | 2.12 | -6.10 | -1.72 | 3.91 | 9.39 | 5.57 |
Golgi | -13.81 | 14.84 | 11.24 | 22.07 | -3.95 | -15.21 | 8.13 | 10.42 | 22.98 | 4.97 | -20.91 | 16.49 | 15.97 | 34.20 | 1.52 |
Lipid Particles | 6.22 | 4.29 | 4.91 | -1.98 | 1.53 | 8.51 | 8.50 | 7.43 | -1.36 | -1.22 | 10.48 | 9.32 | 8.84 | -2.12 | 0.28 |
Mitochondria | -6.96 | 33.06* | 32.41* | 40.32* | 1.29 | -2.59 | 18.20 | 33.06* | 37.87* | 18.96* | -7.67 | 36.33 | 50.38* | 63.78* | 18.19* |
Mitotic Spindle | -4.75 | -21.29* | -14.70 | -12.66 | 1.17 | -4.35 | -17.20 | -3.72 | -3.20 | 2.24 | -6.32 | -27.35 | -15.24 | -13.69 | 0.69 |
None | 1.95 | -0.90 | 1.85 | -0.44 | 3.10 | 4.65 | 4.11 | 4.54 | -0.32 | 2.39 | 5.24 | 3.02 | 4.11 | -1.33 | 2.29 |
Nuclear Periphery | 0.25 | -11.12 | -4.85 | -4.79 | 6.94 | 2.46 | -3.03 | -1.28 | -3.03 | 1.27 | 2.08 | -11.34 | -4.45 | -5.11 | 5.37 |
Nuclear Periphery Foci | 0.70 | -7.17 | -1.74 | -1.88 | 0.65 | 2.54 | -1.27 | -2.77 | -4.50 | -2.10 | 2.65 | -6.77 | -1.93 | -2.38 | -0.22 |
Nucleolus | -0.45 | -9.17 | -2.45 | -1.51 | 7.52 | 3.67 | 0.17 | 2.81 | -1.21 | 3.66 | 2.91 | -5.94 | 1.00 | -2.29 | 7.70 |
Nucleus | 6.13 | -27.49 | -11.58 | -14.23 | 16.73 | 7.14 | -3.66 | -3.63 | -4.77 | -2.71 | 9.60 | -25.54 | -11.98 | -15.24 | 9.96 |
Peroxisomes | -3.66 | 1.81 | -10.95 | -7.67 | -12.35 | 0.77 | -3.15 | -5.72 | -5.89 | -5.00 | -1.78 | -1.02 | -12.23 | -11.27 | -11.81 |
Vacuole | 3.41 | -30.28 | -23.53 | -24.96 | -3.66 | 4.41 | -21.40 | -10.11 | -10.60 | -4.54 | 5.65 | -36.81 | -25.89 | -27.53 | -6.19 |
Vacuole Periphery | -3.23 | -2.44 | 4.50 | 6.68 | 6.87 | 1.86 | -6.21 | 1.82 | 1.13 | 5.31 | -1.09 | -6.12 | 7.66 | 8.83 | 12.19 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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