Standard name
Description RNA-binding protein that activates mRNA decapping directly; binds to mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; EDC1 has a paralog, EDC2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.1 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.97 0.98 0.93 0.89 0.8 0.79 0.71 0.69 0.99 0.99 0.99 0.92 0.93 0.94 0.95 0.95 0.94 0.97 0.98 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.1 0 0.2 0.24 0.33 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.09 0.08 0.06 0.07 0.07 0 0 0 0.06 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 1 0 2 0 2 2 1 0 1 0 0 1 0 0 1
Bud 0 1 0 2 1 6 0 3 4 6 14 0 1 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 2 3 6 0 3 2 4 3 6 5 13 18 16 28 23 0 0 0 0 0 0
Cytoplasm 295 233 153 184 261 208 311 251 246 234 230 261 429 509 252 321 226 285 226 146 105 320 345
Endoplasmic Reticulum 1 1 0 1 0 0 0 1 2 1 7 0 3 2 9 10 13 2 0 1 0 3 3
Endosome 1 0 0 1 0 0 1 1 2 6 2 1 2 1 2 2 2 2 1 1 0 0 1
Golgi 1 0 1 0 1 0 0 0 0 0 0 0 0 0 2 1 0 2 1 0 0 0 1
Mitochondria 2 2 0 9 2 23 14 63 75 108 99 0 0 0 4 3 2 3 2 1 0 0 2
Nucleus 0 0 2 1 1 4 6 3 4 4 9 0 1 1 2 2 0 0 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 2 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 5 0 0 3 3 3 33 24 18 24 22 1 0 5 16 24 13 0 0 0 0 1 2
Unique Cell Count 301 235 156 190 265 224 348 314 310 328 335 263 432 515 273 345 240 302 239 156 109 328 366
Labelled Cell Count 306 238 158 205 275 246 369 348 358 389 393 268 452 538 304 391 280 302 239 156 109 328 366


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.7 7.1 7.2 8.2 5.3 6.5 5.6 5.6 5.6 5.5 8.7 9.0 9.5 11.8 12.1 12.0 6.6 7.2 7.4
Std Deviation (1e-4) 0.9 0.8 1.2 1.2 1.3 1.3 1.3 1.1 1.1 1.1 0.9 1.0 1.2 1.3 2.2 2.4 2.7 0.7 1.2 1.3
Intensity Change (Log2) 0.02 0.2 -0.42 -0.13 -0.36 -0.34 -0.35 -0.38 0.28 0.34 0.41 0.73 0.76 0.75 -0.12 0.02 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 2.3 3.0 3.3
Cytoplasm -0.7 0.3 -2.3 -3.3 -5.3 -5.4 -6.9 -7.3 1.1 1.3 0.7 -2.5 -2.3 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 0 6.0 6.7 8.1 7.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.0 3.5 3.1 3.5 3.3 0 0 0 3.1 3.4 3.0