Standard name | |
---|---|
Description | RNA-binding protein that activates mRNA decapping directly; binds to mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; EDC1 has a paralog, EDC2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 2 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | 1 | 0 | 2 | 1 | 6 | 0 | – | 3 | 4 | 6 | 14 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 3 | 6 | 0 | 3 | – | 2 | 4 | 3 | 6 | 5 | 13 | 18 | 16 | 28 | 23 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 295 | 233 | 153 | 184 | 261 | 208 | 311 | – | 251 | 246 | 234 | 230 | 261 | 429 | 509 | 252 | 321 | 226 | 285 | 226 | 146 | 105 | 320 | 345 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | 1 | 2 | 1 | 7 | 0 | 3 | 2 | 9 | 10 | 13 | 2 | 0 | 1 | 0 | 3 | 3 |
Endosome | 1 | 0 | 0 | 1 | 0 | 0 | 1 | – | 1 | 2 | 6 | 2 | 1 | 2 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 0 | 0 | 1 |
Golgi | 1 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 2 | 2 | 0 | 9 | 2 | 23 | 14 | – | 63 | 75 | 108 | 99 | 0 | 0 | 0 | 4 | 3 | 2 | 3 | 2 | 1 | 0 | 0 | 2 |
Nucleus | 0 | 0 | 2 | 1 | 1 | 4 | 6 | – | 3 | 4 | 4 | 9 | 0 | 1 | 1 | 2 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 5 | 0 | 0 | 3 | 3 | 3 | 33 | – | 24 | 18 | 24 | 22 | 1 | 0 | 5 | 16 | 24 | 13 | 0 | 0 | 0 | 0 | 1 | 2 |
Unique Cell Count | 301 | 235 | 156 | 190 | 265 | 224 | 348 | 314 | 310 | 328 | 335 | 263 | 432 | 515 | 273 | 345 | 240 | 302 | 239 | 156 | 109 | 328 | 366 | |
Labelled Cell Count | 306 | 238 | 158 | 205 | 275 | 246 | 369 | 348 | 358 | 389 | 393 | 268 | 452 | 538 | 304 | 391 | 280 | 302 | 239 | 156 | 109 | 328 | 366 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 6.7 | 7.1 | 7.2 | 8.2 | 5.3 | 6.5 | – | 5.6 | 5.6 | 5.6 | 5.5 | 8.7 | 9.0 | 9.5 | 11.8 | 12.1 | 12.0 | 6.6 | 7.2 | 7.4 |
Std Deviation (1e-4) | 0.9 | 0.8 | 1.2 | 1.2 | 1.3 | 1.3 | 1.3 | – | 1.1 | 1.1 | 1.1 | 0.9 | 1.0 | 1.2 | 1.3 | 2.2 | 2.4 | 2.7 | 0.7 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.2 | -0.42 | -0.13 | – | -0.36 | -0.34 | -0.35 | -0.38 | 0.28 | 0.34 | 0.41 | 0.73 | 0.76 | 0.75 | -0.12 | 0.02 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 3.0 | 3.3 |
Cytoplasm | -0.7 | 0.3 | -2.3 | -3.3 | – | -5.3 | -5.4 | -6.9 | -7.3 | 1.1 | 1.3 | 0.7 | -2.5 | -2.3 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.1 | 0 | – | 6.0 | 6.7 | 8.1 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 4.0 | – | 3.5 | 3.1 | 3.5 | 3.3 | 0 | 0 | 0 | 3.1 | 3.4 | 3.0 |