Standard name
Human Ortholog
Description Cytosolic protein involved in repression of iron regulon transcription; forms an iron-independent complex with Fra1p, Grx3p, and Grx4p; null mutant fails to repress the iron regulon and is sensitive to nickel; sequence similarity to human BOLA family member, BOLA2
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.93 0.9 0.89 – 0.67 0.68 0.65 0.64 0.64 0.98 0.98 0.97 0.92 0.97 0.9 0.92 0.89 0.92 0.92 0.86 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 – 0.18 0.34 0.34 0.37 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0.05 0.05 0.07 0.06 0 0 0 0 0 0 0 0.05 0 0 0 0.05
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.1 0.07 – 0.21 0.11 0.1 0.09 0.06 0 0 0 0.08 0.1 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 3 0 1 0 – 5 4 2 5 3 0 0 0 0 0 0 4 3 0 0 3 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 1 1 – 7 5 9 3 6 1 1 4 1 0 2 0 0 0 0 0 0
Cytoplasm 448 355 298 278 328 431 – 428 294 273 239 271 346 481 409 167 97 140 439 320 288 194 202 245
Endoplasmic Reticulum 1 2 2 1 3 0 – 2 2 2 4 4 3 3 1 8 2 2 1 3 1 0 3 2
Endosome 0 0 0 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 1 1 4 0 2 3
Golgi 0 1 1 0 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 2 1
Mitochondria 1 0 3 13 11 50 – 117 146 144 137 165 0 0 0 3 1 1 0 2 0 0 0 0
Nucleus 3 3 5 3 2 11 – 28 20 19 25 24 8 11 3 5 1 4 18 18 5 6 9 15
Nuclear Periphery 0 0 1 0 0 1 – 4 0 2 2 1 0 0 0 0 0 1 0 1 0 0 0 0
Nucleolus 0 0 0 1 0 0 – 2 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0
Vac/Vac Membrane 21 9 3 22 36 35 – 135 48 44 33 27 1 1 12 15 10 19 3 4 5 6 7 6
Unique Cell Count 468 362 307 299 365 484 640 430 420 372 421 353 490 421 181 100 156 477 361 313 211 235 286
Labelled Cell Count 476 372 316 318 383 529 728 519 496 448 502 359 498 430 200 111 170 477 361 313 211 235 286


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 11.1 6.8 8.2 8.5 7.0 – 6.8 6.0 6.1 5.8 5.9 11.3 11.0 11.0 12.4 12.2 11.5 10.6 10.9 11.0
Std Deviation (1e-4) 1.6 2.1 1.4 1.9 2.0 1.7 – 1.4 1.5 1.3 1.4 1.4 2.3 2.0 2.0 2.8 3.4 2.5 2.1 2.1 2.2
Intensity Change (Log2) – – – 0.27 0.32 0.03 – -0.01 -0.18 -0.15 -0.25 -0.2 0.73 0.68 0.69 0.87 0.84 0.75 0.63 0.68 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 -3.7 -4.1 – -10.3 -9.7 -10.4 -10.5 -10.5 0.8 1.0 0.1 -2.4 0 -3.3
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 1.8 5.1 – 7.5 11.0 11.0 11.5 12.1 0 0 0 0 0 0
Nucleus 0 0 0.6 – 2.2 2.2 2.2 3.2 2.8 0.6 0.6 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.9 4.9 4.0 – 8.2 5.4 5.1 4.6 3.6 0 0 1.8 4.1 0 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8117 5.8221 4.9327 4.121 4.5216 5.0704 3.8821 4.7352 4.3324 4.5265 4.191 4.3909 6.342 6.9105 6.1045 6.44 5.8539 6.4045
Actin 0.0102 0.0004 0.0144 0.0127 0.0103 0.0011 0.0234 0.0092 0.0306 0.0232 0.1132 0.0107 0.0234 0.0005 0.0117 0.0044 0.0033 0.0017
Bud 0.0011 0.0003 0.0005 0.0011 0.0004 0.0001 0.0003 0.0008 0.001 0.0011 0.0044 0.0007 0.0003 0.0001 0.0073 0.0003 0.0209 0.0006
Bud Neck 0.0014 0.0004 0.0019 0.0011 0.0013 0.0017 0.001 0.0004 0.0059 0.0023 0.0081 0.0017 0.0026 0.0003 0.0008 0.0011 0.0017 0.0025
Bud Periphery 0.0007 0.0001 0.0003 0.0013 0.0004 0.0001 0.0002 0.0003 0.0008 0.0006 0.0065 0.0007 0.0002 0 0.0022 0.0002 0.0088 0.0004
Bud Site 0.0054 0.0008 0.0026 0.0024 0.001 0.0001 0.001 0.0031 0.0078 0.0086 0.0094 0.0013 0.0007 0.0012 0.0014 0.0004 0.0015 0.0004
Cell Periphery 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0003 0.0003 0.0006 0 0.0001 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.8272 0.8982 0.8507 0.8204 0.7844 0.8478 0.8373 0.913 0.839 0.8607 0.5182 0.8452 0.8367 0.9306 0.8643 0.8078 0.7942 0.865
Cytoplasmic Foci 0.0179 0.0023 0.012 0.0084 0.012 0.0067 0.029 0.0115 0.017 0.0191 0.0287 0.0097 0.0059 0.0025 0.0089 0.0028 0.019 0.0029
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0093 0.0018 0.0029 0.0036 0.0072 0.0025 0.0057 0.0018 0.0031 0.0073 0.0168 0.0023 0.0054 0.0016 0.0033 0.0045 0.0038 0.0032
Endosome 0.0175 0.0013 0.008 0.0087 0.0226 0.0051 0.0158 0.0023 0.0086 0.0133 0.0794 0.0046 0.0108 0.0007 0.0067 0.0062 0.0054 0.0109
Golgi 0.0027 0.0001 0.0024 0.0031 0.0062 0.0006 0.0046 0.0016 0.0082 0.0037 0.0391 0.0038 0.0014 0.0001 0.0012 0.0005 0.0004 0.0035
Lipid Particles 0.0016 0 0.0035 0.0016 0.0164 0.0006 0.0041 0.0014 0.0039 0.0021 0.0234 0.0026 0.0005 0 0.0008 0.0008 0.0003 0.0008
Mitochondria 0.0029 0.0003 0.0028 0.0017 0.0039 0.0003 0.0009 0.0004 0.0026 0.0017 0.019 0.0256 0.0007 0.0001 0.0016 0.0008 0.0019 0.0008
None 0.0206 0.0126 0.0166 0.0239 0.0071 0.0271 0.031 0.0245 0.0242 0.0167 0.0046 0.0052 0.0281 0.0163 0.0167 0.0101 0.0336 0.0046
Nuclear Periphery 0.0139 0.0039 0.0055 0.0051 0.0146 0.0044 0.007 0.0017 0.003 0.0044 0.0128 0.0039 0.0081 0.0023 0.0051 0.0213 0.0068 0.0079
Nucleolus 0.0003 0.0001 0.0005 0.0003 0.001 0.0002 0.0002 0.0001 0.0003 0.0002 0.0126 0.0002 0.0003 0 0.0004 0.0012 0.001 0.0002
Nucleus 0.0539 0.0738 0.0599 0.0962 0.0766 0.0925 0.0254 0.0217 0.034 0.0207 0.0641 0.0694 0.0609 0.0417 0.0611 0.1293 0.0684 0.0898
Peroxisomes 0.0043 0.0001 0.0082 0.0006 0.0118 0.0002 0.0039 0.0039 0.0048 0.0056 0.0249 0.0076 0.0004 0.0001 0.0011 0.0001 0.0014 0.0002
Punctate Nuclear 0.0055 0.0025 0.0056 0.0058 0.02 0.008 0.0071 0.0017 0.0037 0.0064 0.0052 0.0034 0.0115 0.0014 0.0044 0.0045 0.0253 0.0033
Vacuole 0.0025 0.0008 0.001 0.0014 0.0018 0.0008 0.0013 0.0006 0.0009 0.0017 0.0065 0.0009 0.0013 0.0003 0.0007 0.0022 0.0016 0.0009
Vacuole Periphery 0.0008 0.0001 0.0003 0.0004 0.0008 0.0002 0.0003 0.0001 0.0002 0.0004 0.0022 0.0003 0.0004 0.0001 0.0002 0.0011 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.3928 97.8224 50.4087 71.003 66.9984 69.874 67.9994 74.1737 81.7919 79.5095
Translational Efficiency 1.3565 0.9281 1.5664 1.0353 1.2268 1.5118 1.3406 1.157 1.1955 1.0603

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytosolic protein involved in repression of iron regulon transcription; forms an iron-independent complex with Fra1p, Grx3p, and Grx4p; null mutant fails to repress the iron regulon and is sensitive to nickel; sequence similarity to human BOLA family member, BOLA2
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bol2

Bol2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bol2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available