Standard name | |
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Description | Cytosolic protein involved in repression of iron regulon transcription; forms an iron-independent complex with Fra1p, Grx3p, and Grx4p; null mutant fails to repress the iron regulon and is sensitive to nickel; sequence similarity to human BOLA family member, BOLA2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | 3 | 0 | 1 | 0 | – | 5 | 4 | 2 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 0 | 0 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 0 | 1 | 1 | – | 7 | 5 | 9 | 3 | 6 | 1 | 1 | 4 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 448 | 355 | 298 | 278 | 328 | 431 | – | 428 | 294 | 273 | 239 | 271 | 346 | 481 | 409 | 167 | 97 | 140 | 439 | 320 | 288 | 194 | 202 | 245 |
Endoplasmic Reticulum | 1 | 2 | 2 | 1 | 3 | 0 | – | 2 | 2 | 2 | 4 | 4 | 3 | 3 | 1 | 8 | 2 | 2 | 1 | 3 | 1 | 0 | 3 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 2 | 3 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
Mitochondria | 1 | 0 | 3 | 13 | 11 | 50 | – | 117 | 146 | 144 | 137 | 165 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Nucleus | 3 | 3 | 5 | 3 | 2 | 11 | – | 28 | 20 | 19 | 25 | 24 | 8 | 11 | 3 | 5 | 1 | 4 | 18 | 18 | 5 | 6 | 9 | 15 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 1 | – | 4 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 21 | 9 | 3 | 22 | 36 | 35 | – | 135 | 48 | 44 | 33 | 27 | 1 | 1 | 12 | 15 | 10 | 19 | 3 | 4 | 5 | 6 | 7 | 6 |
Unique Cell Count | 468 | 362 | 307 | 299 | 365 | 484 | 640 | 430 | 420 | 372 | 421 | 353 | 490 | 421 | 181 | 100 | 156 | 477 | 361 | 313 | 211 | 235 | 286 | |
Labelled Cell Count | 476 | 372 | 316 | 318 | 383 | 529 | 728 | 519 | 496 | 448 | 502 | 359 | 498 | 430 | 200 | 111 | 170 | 477 | 361 | 313 | 211 | 235 | 286 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.8 | 11.1 | 6.8 | 8.2 | 8.5 | 7.0 | – | 6.8 | 6.0 | 6.1 | 5.8 | 5.9 | 11.3 | 11.0 | 11.0 | 12.4 | 12.2 | 11.5 | 10.6 | 10.9 | 11.0 |
Std Deviation (1e-4) | 1.6 | 2.1 | 1.4 | 1.9 | 2.0 | 1.7 | – | 1.4 | 1.5 | 1.3 | 1.4 | 1.4 | 2.3 | 2.0 | 2.0 | 2.8 | 3.4 | 2.5 | 2.1 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.27 | 0.32 | 0.03 | – | -0.01 | -0.18 | -0.15 | -0.25 | -0.2 | 0.73 | 0.68 | 0.69 | 0.87 | 0.84 | 0.75 | 0.63 | 0.68 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.3 | -3.7 | -4.1 | – | -10.3 | -9.7 | -10.4 | -10.5 | -10.5 | 0.8 | 1.0 | 0.1 | -2.4 | 0 | -3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.6 | 1.8 | 5.1 | – | 7.5 | 11.0 | 11.0 | 11.5 | 12.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0.6 | – | 2.2 | 2.2 | 2.2 | 3.2 | 2.8 | 0.6 | 0.6 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.9 | 4.9 | 4.0 | – | 8.2 | 5.4 | 5.1 | 4.6 | 3.6 | 0 | 0 | 1.8 | 4.1 | 0 | 5.4 |