Standard name
Description Cytosolic protein involved in repression of iron regulon transcription; forms an iron-independent complex with Fra1p, Grx3p, and Grx4p; null mutant fails to repress the iron regulon and is sensitive to nickel; sequence similarity to human BOLA family member, BOLA2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.93 0.9 0.89 – 0.67 0.68 0.65 0.64 0.64 0.98 0.98 0.97 0.92 0.97 0.9 0.92 0.89 0.92 0.92 0.86 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 – 0.18 0.34 0.34 0.37 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0.05 0.05 0.07 0.06 0 0 0 0 0 0 0 0.05 0 0 0 0.05
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.1 0.07 – 0.21 0.11 0.1 0.09 0.06 0 0 0 0.08 0.1 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 3 0 1 0 – 5 4 2 5 3 0 0 0 0 0 0 4 3 0 0 3 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 1 1 – 7 5 9 3 6 1 1 4 1 0 2 0 0 0 0 0 0
Cytoplasm 448 355 298 278 328 431 – 428 294 273 239 271 346 481 409 167 97 140 439 320 288 194 202 245
Endoplasmic Reticulum 1 2 2 1 3 0 – 2 2 2 4 4 3 3 1 8 2 2 1 3 1 0 3 2
Endosome 0 0 0 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 1 1 4 0 2 3
Golgi 0 1 1 0 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 2 1
Mitochondria 1 0 3 13 11 50 – 117 146 144 137 165 0 0 0 3 1 1 0 2 0 0 0 0
Nucleus 3 3 5 3 2 11 – 28 20 19 25 24 8 11 3 5 1 4 18 18 5 6 9 15
Nuclear Periphery 0 0 1 0 0 1 – 4 0 2 2 1 0 0 0 0 0 1 0 1 0 0 0 0
Nucleolus 0 0 0 1 0 0 – 2 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0
Vac/Vac Membrane 21 9 3 22 36 35 – 135 48 44 33 27 1 1 12 15 10 19 3 4 5 6 7 6
Unique Cell Count 468 362 307 299 365 484 640 430 420 372 421 353 490 421 181 100 156 477 361 313 211 235 286
Labelled Cell Count 476 372 316 318 383 529 728 519 496 448 502 359 498 430 200 111 170 477 361 313 211 235 286


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 11.1 6.8 8.2 8.5 7.0 – 6.8 6.0 6.1 5.8 5.9 11.3 11.0 11.0 12.4 12.2 11.5 10.6 10.9 11.0
Std Deviation (1e-4) 1.6 2.1 1.4 1.9 2.0 1.7 – 1.4 1.5 1.3 1.4 1.4 2.3 2.0 2.0 2.8 3.4 2.5 2.1 2.1 2.2
Intensity Change (Log2) – – – 0.27 0.32 0.03 – -0.01 -0.18 -0.15 -0.25 -0.2 0.73 0.68 0.69 0.87 0.84 0.75 0.63 0.68 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 -3.7 -4.1 – -10.3 -9.7 -10.4 -10.5 -10.5 0.8 1.0 0.1 -2.4 0 -3.3
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 1.8 5.1 – 7.5 11.0 11.0 11.5 12.1 0 0 0 0 0 0
Nucleus 0 0 0.6 – 2.2 2.2 2.2 3.2 2.8 0.6 0.6 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.9 4.9 4.0 – 8.2 5.4 5.1 4.6 3.6 0 0 1.8 4.1 0 5.4