Standard name
Human Ortholog
Description Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.99 0.98 0.9 0.81 0.76 0.65 0.64 0.56 0.58 0.99 1.0 0.99 0.97 0.97 0.94 0.97 0.94 0.93 0.93 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.09 0 0.23 0.35 0.27 0.4 0.52 0.61 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.09 0.11 0.11 0.08 0.07 0 0 0 0 0 0.05 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 5
Bud 0 0 0 1 1 2 4 4 6 12 7 19 0 0 0 0 0 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 2
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 1 3 5 4 2 1 3 1 4 6 5 10 2 1 2 4 4 7 0 0 0 0 0 0
Cytoplasm 130 113 211 227 325 268 410 337 212 313 173 232 265 342 337 145 113 90 128 112 191 107 236 242
Endoplasmic Reticulum 0 1 1 1 1 1 1 2 0 1 0 0 1 1 1 11 9 9 0 0 0 0 1 2
Endosome 0 0 1 0 0 0 1 1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 2 0 0 1 0 0 0
Mitochondria 8 1 1 21 14 67 175 121 129 251 187 215 1 0 0 2 0 3 1 0 3 0 1 1
Nucleus 0 0 0 1 1 3 5 5 7 6 1 8 0 2 0 2 3 1 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 1 3 0 2 0 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 2 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 2 3 3 4 23 38 36 56 26 29 0 1 0 3 1 5 0 5 2 0 1 0
Unique Cell Count 132 115 214 230 330 297 504 445 324 487 309 400 268 343 339 149 116 96 133 120 206 115 256 266
Labelled Cell Count 139 118 221 258 347 346 624 514 400 649 400 515 270 347 342 167 130 118 133 120 206 115 256 266


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.7 5.5 5.1 5.2 4.8 4.6 4.7 4.7 4.4 4.1 4.1 6.4 6.5 6.5 7.0 7.1 7.0 7.7 8.8 10.3
Std Deviation (1e-4) 0.8 0.8 1.1 1.5 1.3 1.5 1.4 1.5 1.6 1.3 1.4 0.9 0.9 1.2 1.2 1.4 1.4 1.7 1.4 1.5 1.5
Intensity Change (Log2) -0.11 -0.07 -0.21 -0.25 -0.21 -0.23 -0.3 -0.41 -0.41 0.21 0.24 0.23 0.35 0.38 0.36 0.48 0.67 0.91


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -0.1 -3.8 -6.2 -7.3 -9.2 -9.6 -10.8 -10.7 0.3 1.5 1.0 -0.9 -0.8 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.2 2.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.4 3.8 4.5 4.7 3.7 3.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8376 3.0562 2.917 2.5279 2.8798 2.9972 0.803 1.4763 1.5447 0.9567 0.9276 1.3484 0.1107 0.5783 0.1875 0.2206 -0.0693 0.3517
Actin 0.0323 0.0019 0.0264 0.0252 0.0011 0.001 0.0323 0.002 0.0097 0.0002 0.0024 0.0026 0.0418 0.0002 0.006 0.0131 0.0326 0.0071
Bud 0.0007 0.0017 0.0012 0.0004 0.009 0.0002 0.0084 0.0008 0.0122 0.0001 0.0264 0.0096 0.0006 0.0001 0.0011 0.0005 0.001 0.0003
Bud Neck 0.002 0.0005 0.0022 0.0002 0.0003 0.0013 0.0013 0.0028 0.0009 0.0002 0.0014 0.0027 0.0087 0.0001 0.002 0.0031 0.0005 0.0013
Bud Periphery 0.0012 0.001 0.0017 0.0005 0.0039 0.0002 0.0087 0.0008 0.006 0 0.0367 0.009 0.0006 0 0.0013 0.0004 0.0016 0.0004
Bud Site 0.0016 0.0054 0.0036 0.001 0.0002 0.0001 0.012 0.0103 0.0208 0.0001 0.0207 0.0087 0.0066 0.0002 0.0074 0.0085 0.0029 0.0002
Cell Periphery 0.0002 0.0005 0.0003 0.0001 0.0003 0.0001 0.0004 0.0003 0.0003 0 0.0008 0.0005 0.0002 0 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.6369 0.702 0.7128 0.8222 0.5442 0.7563 0.5796 0.8428 0.7452 0.9139 0.7163 0.7973 0.6728 0.7482 0.7821 0.7436 0.7371 0.8001
Cytoplasmic Foci 0.0277 0.0134 0.0185 0.0106 0.0067 0.0192 0.0416 0.0047 0.0219 0.0038 0.0067 0.019 0.0148 0.0042 0.0173 0.023 0.034 0.0261
Eisosomes 0.0002 0.0001 0.0005 0.0002 0 0 0.0002 0.0001 0.0001 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0094 0.0025 0.0024 0.0031 0.0032 0.0051 0.0044 0.0018 0.0045 0.0026 0.0039 0.0041 0.0062 0.0027 0.0036 0.0041 0.0018 0.0035
Endosome 0.0186 0.0013 0.0068 0.0074 0.0032 0.003 0.042 0.0014 0.0487 0.0024 0.0397 0.0209 0.0173 0.0015 0.0081 0.0161 0.0141 0.0087
Golgi 0.007 0.0003 0.0026 0.0038 0.0002 0.0002 0.0102 0.0008 0.0158 0.0001 0.0105 0.0093 0.0061 0.0002 0.0012 0.0056 0.004 0.0014
Lipid Particles 0.0062 0.0003 0.0058 0.0122 0.0001 0.0002 0.0162 0.0003 0.0133 0 0.0083 0.0017 0.0037 0.0002 0.0011 0.0034 0.0454 0.0016
Mitochondria 0.0024 0.0003 0.0045 0.0038 0.0005 0.0002 0.0105 0.0072 0.0284 0.0001 0.0544 0.0043 0.0019 0 0.0006 0.0006 0.0082 0.0005
None 0.1928 0.2522 0.1878 0.0874 0.4175 0.1912 0.1921 0.1207 0.0512 0.0718 0.0418 0.0745 0.1935 0.2399 0.149 0.1644 0.0715 0.1263
Nuclear Periphery 0.0128 0.0007 0.0018 0.0016 0.0016 0.0015 0.0057 0.0005 0.0016 0.001 0.0026 0.0079 0.0143 0.0006 0.0023 0.0021 0.0023 0.0069
Nucleolus 0.0009 0.0007 0.0008 0.0001 0.0003 0.0002 0.0022 0.0001 0.0003 0 0.0016 0.0009 0.0005 0 0.0008 0.0003 0.0014 0.0003
Nucleus 0.024 0.0092 0.0026 0.0024 0.0034 0.0161 0.0059 0.001 0.0019 0.0018 0.0074 0.0108 0.0026 0.001 0.0038 0.0021 0.0023 0.0031
Peroxisomes 0.0085 0.0005 0.012 0.0143 0.0002 0.0002 0.0139 0.0003 0.0049 0 0.001 0.0007 0.0019 0 0.0016 0.0044 0.0262 0.0028
Punctate Nuclear 0.0124 0.0048 0.0043 0.0019 0.0015 0.003 0.0044 0.0005 0.0014 0.0003 0.0006 0.0036 0.0029 0.0004 0.0093 0.0024 0.0103 0.0082
Vacuole 0.0015 0.0009 0.0011 0.0012 0.0021 0.0007 0.0067 0.0006 0.0082 0.0014 0.0122 0.0086 0.0014 0.0005 0.0013 0.0017 0.0019 0.0007
Vacuole Periphery 0.0008 0.0001 0.0003 0.0004 0.0003 0.0001 0.0015 0.0001 0.0029 0.0002 0.0049 0.003 0.0011 0 0.0002 0.0003 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.6548 5.7539 6.0994 12.8457 8.9413 13.7849 11.2199 12.9016 9.809 13.1991
Translational Efficiency 0.7558 1.0748 1.2248 0.639 0.9072 0.7056 0.8014 0.7837 1.1306 0.9611

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
483 196 671 1083 782 764 218 978 1265 960 889 2061

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 683.74 790.87 1007.90 918.14 793.83 760.02 1088.49 860.58 751.80 766.32 1027.66 890.83
Standard Deviation 102.48 108.79 127.72 126.50 105.68 96.64 109.89 132.18 117.37 100.02 128.36 132.39
Intensity Change Log 2 0.209993 0.559833 0.425266 -0.062793 0.455426 0.116479 0.069870 0.504681 0.267613

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000247 0.000560 0.000657 0.001682 0.000081 0.000810 0.000731 0.000597 0.000144 0.000759 0.000675 0.001167
Bud Neck 0.014178 0.021096 0.003322 0.006184 0.002072 0.022644 0.002563 0.010627 0.006694 0.022328 0.003136 0.008292
Bud Site 0.005381 0.018024 0.005916 0.014798 0.002872 0.013162 0.005796 0.019016 0.003830 0.014155 0.005886 0.016800
Cell Periphery 0.000243 0.000217 0.000245 0.000239 0.000190 0.000172 0.000295 0.000223 0.000211 0.000181 0.000257 0.000231
Cytoplasm 0.463102* 0.461654* 0.442503* 0.476512* 0.717145* 0.459918* 0.368321* 0.513088* 0.620147* 0.460272* 0.424312* 0.493868*
Cytoplasmic Foci 0.289489* 0.270917* 0.014522 0.025458 0.122255 0.239667* 0.017624 0.043123 0.186108* 0.246047* 0.015282 0.033840
Eisosomes 0.000233 0.000143 0.000054 0.000072 0.000060 0.000153 0.000101 0.000082 0.000126 0.000151 0.000066 0.000077
Endoplasmic Reticulum 0.000895 0.000908 0.006815 0.004510 0.001523 0.000841 0.008243 0.002119 0.001284 0.000854 0.007165 0.003375
Endosome 0.018061 0.022855 0.003930 0.009100 0.005448 0.031391 0.006345 0.013712 0.010264 0.029648 0.004522 0.011288
Golgi 0.003526 0.002021 0.000134 0.005262 0.000482 0.005159 0.000139 0.004515 0.001644 0.004518 0.000135 0.004907
Lipid Particles 0.008992 0.003836 0.002114 0.001808 0.002227 0.007077 0.003435 0.003007 0.004810 0.006415 0.002438 0.002377
Mitochondria 0.006843 0.002828 0.000390 0.004142 0.000224 0.010051 0.000259 0.005981 0.002752 0.008577 0.000358 0.005015
Mitotic Spindle 0.000657 0.001260 0.004426 0.019139 0.000957 0.008868 0.000737 0.022409 0.000842 0.007314 0.003522 0.020691
None 0.002908 0.001742 0.003530 0.007453 0.005287 0.001036 0.001683 0.004430 0.004379 0.001180 0.003077 0.006018
Nuclear Periphery 0.000318 0.000224 0.001730 0.002745 0.000195 0.000324 0.001669 0.001294 0.000242 0.000304 0.001715 0.002056
Nuclear Periphery Foci 0.000670 0.001272 0.005079 0.002825 0.001758 0.000424 0.008572 0.004691 0.001343 0.000597 0.005936 0.003710
Nucleolus 0.001406 0.000954 0.000193 0.000584 0.000347 0.001052 0.000223 0.000683 0.000751 0.001032 0.000200 0.000631
Nucleus 0.119766 0.066680 0.373302* 0.228524* 0.095819 0.070948 0.271506* 0.152136* 0.104962 0.070076 0.348340* 0.192276*
Peroxisomes 0.006553 0.012311 0.000226 0.001699 0.000762 0.008609 0.000343 0.004185 0.002973 0.009365 0.000255 0.002878
Vacuole 0.055209 0.109308 0.130746 0.182816 0.039742 0.115239 0.301231* 0.192026 0.045647 0.114028 0.172552 0.187187
Vacuole Periphery 0.001322 0.001189 0.000166 0.004449 0.000553 0.002457 0.000185 0.002058 0.000847 0.002198 0.000171 0.003315

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.17 -6.79 -2.71 -1.40 -0.70 -1.47 -9.82 -5.42 0.69 3.91 -1.55 -12.31 -4.06 0.06 0.50
Bud Neck -1.20 4.37 4.04 3.18 -0.39 -8.57 -3.53 -3.44 5.81 -2.46 -6.74 2.71 0.80 7.25 -1.92
Bud Site -4.18 -1.14 -3.24 2.05 -2.80 -5.66 -5.70 -5.19 0.20 -3.22 -6.44 -4.40 -6.42 1.12 -4.14
Cell Periphery 0.41 -0.43 1.16 1.01 5.10 1.42 -2.95 1.76 0.25 4.11 1.27 -2.13 1.68 0.54 6.76
Cytoplasm 0.04 6.86 6.53 4.56 -0.86 20.31 25.84 23.41 1.85 -8.66 13.67 25.26 22.70 5.61 -5.88
Cytoplasmic Foci 0.99 27.01 26.58 16.33 -2.49 -13.41 21.88 17.82 27.73 -7.59 -7.00 31.75 29.59 33.17 -7.64
Eisosomes 3.33 8.15 8.04 4.03 -0.24 -11.53 -6.63 -4.14 9.45 4.27 -2.32 5.47 6.09 10.60 1.11
Endoplasmic Reticulum -0.04 -14.12 -10.28 -10.49 7.62 2.35 -9.64 -2.03 -5.41 8.71 2.19 -15.78 -7.44 -11.95 11.54
Endosome -1.19 6.48 6.42 5.88 0.50 -11.10 0.39 -1.54 9.20 -1.77 -9.08 6.44 4.25 11.80 -1.69
Golgi 1.78 4.32 1.99 0.59 -2.23 -6.01 8.00 -2.39 3.08 -2.92 -4.12 4.96 -0.24 3.55 -3.63
Lipid Particles 4.50 6.43 7.26 3.39 4.90 -6.18 -2.97 0.10 6.04 2.70 -2.11 4.78 6.35 7.16 3.83
Mitochondria 1.30 2.48 2.04 0.66 -2.34 -4.43 -2.45 -2.87 3.03 -2.79 -2.82 2.36 0.57 3.44 -3.50
Mitotic Spindle -1.18 -2.63 -5.08 -4.64 -2.70 -2.98 -0.14 -4.40 -1.44 -4.53 -3.13 -2.35 -6.50 -2.16 -4.32
None 1.09 0.13 -2.67 -3.47 -3.61 4.52 3.78 1.80 -3.60 -2.49 4.81 2.82 -0.16 -6.30 -3.83
Nuclear Periphery 1.66 -21.08 -4.29 -4.50 0.54 -3.12 -16.63 -6.68 -5.78 4.76 -1.64 -27.12 -6.29 -6.01 2.48
Nuclear Periphery Foci -1.64 -10.65 -8.46 -3.35 6.44 6.51 -7.25 -1.75 -3.49 3.93 4.67 -11.68 -3.73 -5.76 5.90
Nucleolus 0.90 2.43 2.11 3.20 -2.11 -4.18 4.10 -1.13 3.31 -2.68 -1.17 2.84 1.81 4.29 -3.45
Nucleus 5.21 -21.51 -14.48 -18.78 8.71 4.76 -11.44 -9.76 -13.51 6.90 7.29 -27.02 -18.57 -24.78 13.65
Peroxisomes -1.90 5.83 5.34 4.23 -2.17 -6.96 1.49 -2.08 5.50 -3.19 -5.65 5.97 3.20 7.57 -3.90
Vacuole -5.51 -15.27 -24.09 -13.96 -6.94 -12.71 -18.72 -28.34 -17.92 3.92 -13.17 -23.32 -37.68 -23.01 -4.68
Vacuole Periphery 0.40 3.53 -0.03 -0.28 -2.04 -6.04 2.37 -1.10 3.44 -2.37 -4.97 4.22 -0.84 2.55 -2.91