Standard name | |
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Description | Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 5 |
Bud | 0 | 0 | 0 | 1 | 1 | 2 | 4 | 4 | 6 | 12 | 7 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 5 | 4 | 2 | 1 | 3 | 1 | 4 | 6 | 5 | 10 | 2 | 1 | 2 | 4 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 130 | 113 | 211 | 227 | 325 | 268 | 410 | 337 | 212 | 313 | 173 | 232 | 265 | 342 | 337 | 145 | 113 | 90 | 128 | 112 | 191 | 107 | 236 | 242 |
Endoplasmic Reticulum | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 11 | 9 | 9 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 8 | 1 | 1 | 21 | 14 | 67 | 175 | 121 | 129 | 251 | 187 | 215 | 1 | 0 | 0 | 2 | 0 | 3 | 1 | 0 | 3 | 0 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 5 | 7 | 6 | 1 | 8 | 0 | 2 | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 3 | 3 | 4 | 23 | 38 | 36 | 56 | 26 | 29 | 0 | 1 | 0 | 3 | 1 | 5 | 0 | 5 | 2 | 0 | 1 | 0 |
Unique Cell Count | 132 | 115 | 214 | 230 | 330 | 297 | 504 | 445 | 324 | 487 | 309 | 400 | 268 | 343 | 339 | 149 | 116 | 96 | 133 | 120 | 206 | 115 | 256 | 266 |
Labelled Cell Count | 139 | 118 | 221 | 258 | 347 | 346 | 624 | 514 | 400 | 649 | 400 | 515 | 270 | 347 | 342 | 167 | 130 | 118 | 133 | 120 | 206 | 115 | 256 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.7 | 5.5 | 5.1 | 5.2 | 4.8 | 4.6 | 4.7 | 4.7 | 4.4 | 4.1 | 4.1 | 6.4 | 6.5 | 6.5 | 7.0 | 7.1 | 7.0 | 7.7 | 8.8 | 10.3 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.1 | 1.5 | 1.3 | 1.5 | 1.4 | 1.5 | 1.6 | 1.3 | 1.4 | 0.9 | 0.9 | 1.2 | 1.2 | 1.4 | 1.4 | 1.7 | 1.4 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.07 | -0.21 | -0.25 | -0.21 | -0.23 | -0.3 | -0.41 | -0.41 | 0.21 | 0.24 | 0.23 | 0.35 | 0.38 | 0.36 | 0.48 | 0.67 | 0.91 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | -0.1 | -3.8 | -6.2 | -7.3 | -9.2 | -9.6 | -10.8 | -10.7 | 0.3 | 1.5 | 1.0 | -0.9 | -0.8 | -2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.2 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.4 | 3.8 | 4.5 | 4.7 | 3.7 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |