Standard name
Description Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.99 0.98 0.9 0.81 0.76 0.65 0.64 0.56 0.58 0.99 1.0 0.99 0.97 0.97 0.94 0.97 0.94 0.93 0.93 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.09 0 0.23 0.35 0.27 0.4 0.52 0.61 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.09 0.11 0.11 0.08 0.07 0 0 0 0 0 0.05 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 5
Bud 0 0 0 1 1 2 4 4 6 12 7 19 0 0 0 0 0 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 2
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 5 4 2 1 3 1 4 6 5 10 2 1 2 4 4 7 0 0 0 0 0 0
Cytoplasm 130 113 211 227 325 268 410 337 212 313 173 232 265 342 337 145 113 90 128 112 191 107 236 242
Endoplasmic Reticulum 0 1 1 1 1 1 1 2 0 1 0 0 1 1 1 11 9 9 0 0 0 0 1 2
Endosome 0 0 1 0 0 0 1 1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 2 0 0 1 0 0 0
Mitochondria 8 1 1 21 14 67 175 121 129 251 187 215 1 0 0 2 0 3 1 0 3 0 1 1
Nucleus 0 0 0 1 1 3 5 5 7 6 1 8 0 2 0 2 3 1 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 1 3 0 2 0 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 2 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 2 3 3 4 23 38 36 56 26 29 0 1 0 3 1 5 0 5 2 0 1 0
Unique Cell Count 132 115 214 230 330 297 504 445 324 487 309 400 268 343 339 149 116 96 133 120 206 115 256 266
Labelled Cell Count 139 118 221 258 347 346 624 514 400 649 400 515 270 347 342 167 130 118 133 120 206 115 256 266


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.7 5.5 5.1 5.2 4.8 4.6 4.7 4.7 4.4 4.1 4.1 6.4 6.5 6.5 7.0 7.1 7.0 7.7 8.8 10.3
Std Deviation (1e-4) 0.8 0.8 1.1 1.5 1.3 1.5 1.4 1.5 1.6 1.3 1.4 0.9 0.9 1.2 1.2 1.4 1.4 1.7 1.4 1.5 1.5
Intensity Change (Log2) – – – -0.11 -0.07 -0.21 -0.25 -0.21 -0.23 -0.3 -0.41 -0.41 0.21 0.24 0.23 0.35 0.38 0.36 0.48 0.67 0.91


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -0.1 -3.8 -6.2 -7.3 -9.2 -9.6 -10.8 -10.7 0.3 1.5 1.0 -0.9 -0.8 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.2 2.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.4 3.8 4.5 4.7 3.7 3.4 0 0 0 0 0 0