Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 11 | 2 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 1 | 4 | 0 | 3 | 5 | 7 | 4 | 3 | 1 | 9 | 2 | 1 | 1 | 0 |
Bud | 3 | 5 | 13 | 1 | 2 | 15 | 13 | 23 | 12 | 25 | 18 | 20 | 1 | 2 | 3 | 0 | 1 | 0 | 1 | 8 | 3 | 5 | 3 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 82 | 259 | 124 | 78 | 74 | 212 | 202 | 208 | 151 | 221 | 208 | 226 | 94 | 200 | 230 | 224 | 115 | 108 | 54 | 50 | 17 | 29 | 19 | 36 |
Endoplasmic Reticulum | 2 | 15 | 7 | 1 | 6 | 11 | 7 | 6 | 5 | 1 | 8 | 10 | 3 | 0 | 2 | 14 | 7 | 6 | 1 | 3 | 0 | 0 | 4 | 7 |
Endosome | 24 | 236 | 181 | 72 | 84 | 101 | 129 | 124 | 106 | 207 | 72 | 111 | 164 | 220 | 205 | 133 | 85 | 101 | 38 | 432 | 224 | 186 | 230 | 289 |
Golgi | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 8 | 3 | 1 | 1 | 5 | 1 | 0 | 0 | 5 |
Mitochondria | 3 | 3 | 4 | 4 | 19 | 69 | 111 | 144 | 138 | 132 | 180 | 187 | 0 | 0 | 0 | 10 | 5 | 1 | 2 | 5 | 2 | 5 | 5 | 2 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 2 | 2 | 1 |
Nuclear Periphery | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 7 | 6 | 2 | 4 | 8 |
Nucleolus | 3 | 17 | 18 | 4 | 2 | 8 | 12 | 19 | 9 | 21 | 20 | 10 | 16 | 18 | 26 | 11 | 8 | 7 | 0 | 2 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 1 | 4 | 0 | 0 | 5 | 1 | 6 | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 5 | 25 | 21 | 13 | 3 | 10 | 22 | 20 | 23 | 24 | 8 | 12 | 46 | 69 | 47 | 19 | 9 | 14 | 3 | 23 | 18 | 21 | 21 | 44 |
Vac/Vac Membrane | 17 | 305 | 107 | 28 | 47 | 37 | 39 | 56 | 65 | 94 | 59 | 71 | 163 | 171 | 129 | 121 | 75 | 91 | 32 | 173 | 77 | 79 | 60 | 79 |
Unique Cell Count | 124 | 668 | 344 | 158 | 201 | 391 | 439 | 487 | 401 | 585 | 473 | 546 | 356 | 514 | 506 | 406 | 230 | 242 | 144 | 734 | 360 | 339 | 361 | 493 |
Labelled Cell Count | 142 | 881 | 479 | 205 | 239 | 469 | 544 | 609 | 518 | 732 | 579 | 663 | 491 | 684 | 649 | 547 | 313 | 334 | 144 | 734 | 360 | 339 | 361 | 493 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 8.6 | 7.2 | 7.0 | 6.5 | 6.4 | 5.6 | 6.0 | 6.1 | 5.9 | 5.8 | 6.0 | 8.8 | 8.7 | 8.8 | 11.4 | 10.7 | 10.8 | 9.4 | 9.6 | 9.8 |
Std Deviation (1e-4) | 2.4 | 1.1 | 1.0 | 1.2 | 1.2 | 1.0 | 0.9 | 1.0 | 1.3 | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 | 1.0 | 2.0 | 2.1 | 1.7 | 1.3 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.15 | -0.18 | -0.37 | -0.26 | -0.25 | -0.29 | -0.31 | -0.28 | 0.28 | 0.27 | 0.28 | 0.65 | 0.57 | 0.58 | 0.38 | 0.4 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -1.9 | 0 | -0.6 | 0.7 | -0.6 | 0.4 | 0 | -0.1 | -3.3 | -3.7 | -3.4 | -4.0 | -2.5 | -3.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.8 | 0.2 | 4.9 | 2.8 | 1.9 | 0.5 | 0.5 | 2.3 | 1.6 | -2.8 | 0.8 | 2.7 | 5.2 | 3.3 | 2.1 |
Endoplasmic Reticulum | 0 | 0 | 0.7 | -0.5 | -0.9 | -0.9 | 0 | -0.4 | -0.2 | 0 | 0 | 0 | 1.2 | 0.8 | 0.4 |
Endosome | -1.5 | -2.4 | -7.5 | -6.6 | -8.0 | -7.3 | -5.1 | -11.4 | -10.0 | -1.7 | -2.8 | -3.5 | -5.5 | -3.7 | -2.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 4.6 | 7.5 | 9.5 | 10.5 | 11.5 | 8.9 | 12.5 | 11.7 | 0 | 0 | 0 | 1.3 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.4 | -2.5 | -2.3 | -1.8 | -0.9 | -2.2 | -1.2 | -0.7 | -2.8 | -0.5 | -1.2 | -0.1 | -1.8 | -1.0 | -1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.9 | -2.5 | -2.4 | -0.7 | -1.3 | -0.2 | -1.4 | -3.4 | -3.0 | 3.1 | 3.4 | 1.7 | -0.9 | -1.2 | -0.2 |
Vacuole | -3.1 | -1.9 | -7.4 | -7.9 | -7.0 | -4.8 | -5.4 | -6.5 | -6.6 | 4.0 | 0.7 | -1.8 | -0.4 | 0.4 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1686 | 4.0149 | 3.7482 | 3.7297 | 3.3287 | 3.6133 | 5.9904 | 6.756 | 6.8184 | 6.36 | 6.5941 | 6.7711 | 3.8426 | 5.0096 | 4.6721 | 4.7037 | 4.0827 | 4.3499 |
Actin | 0.0038 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0048 | 0.0002 | 0.0002 | 0.0007 | 0 | 0.0004 | 0.0212 | 0.0001 | 0.0145 | 0.0073 | 0.0026 | 0.004 |
Bud | 0.0072 | 0.0088 | 0.0049 | 0.0151 | 0.0007 | 0.0012 | 0.0003 | 0.001 | 0.0007 | 0.0037 | 0.0005 | 0.0004 | 0.0007 | 0.0004 | 0.0005 | 0.0054 | 0.0009 | 0.0005 |
Bud Neck | 0.0022 | 0.0011 | 0.0015 | 0.0004 | 0.0012 | 0.0034 | 0.0006 | 0.0012 | 0.0009 | 0.0004 | 0.0007 | 0.0012 | 0.0031 | 0.001 | 0.0015 | 0.0013 | 0.0008 | 0.0014 |
Bud Periphery | 0.0029 | 0.0011 | 0.0016 | 0.0054 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0.0005 | 0.0014 | 0.0002 | 0.0003 | 0.0009 | 0.0002 | 0.0003 | 0.0038 | 0.0017 | 0.0004 |
Bud Site | 0.0139 | 0.0065 | 0.005 | 0.0018 | 0.0008 | 0.0026 | 0.0009 | 0.0049 | 0.0014 | 0.0009 | 0.0026 | 0.0007 | 0.0049 | 0.0035 | 0.0038 | 0.0063 | 0.0023 | 0.0007 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.2626 | 0.3255 | 0.3033 | 0.2027 | 0.1679 | 0.2782 | 0.1694 | 0.2967 | 0.2316 | 0.2492 | 0.1217 | 0.1616 | 0.1294 | 0.2854 | 0.2327 | 0.2282 | 0.1304 | 0.1986 |
Cytoplasmic Foci | 0.1816 | 0.1855 | 0.2124 | 0.2217 | 0.2639 | 0.2281 | 0.1981 | 0.1999 | 0.2319 | 0.2809 | 0.2178 | 0.2729 | 0.1784 | 0.145 | 0.1571 | 0.1786 | 0.2067 | 0.1982 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0042 | 0.0035 | 0.0031 | 0.0016 | 0.0015 | 0.0028 | 0.0042 | 0.0036 | 0.003 | 0.0024 | 0.0049 | 0.0033 | 0.0041 | 0.0038 | 0.0032 | 0.006 | 0.0033 | 0.0041 |
Endosome | 0.3589 | 0.3294 | 0.3417 | 0.3949 | 0.4253 | 0.3473 | 0.5103 | 0.375 | 0.439 | 0.4074 | 0.53 | 0.4395 | 0.4232 | 0.3526 | 0.4361 | 0.3663 | 0.4461 | 0.4133 |
Golgi | 0.0072 | 0.008 | 0.0101 | 0.0072 | 0.0114 | 0.0076 | 0.0115 | 0.0106 | 0.0133 | 0.0151 | 0.0102 | 0.0149 | 0.0238 | 0.0096 | 0.0125 | 0.0064 | 0.0098 | 0.0107 |
Lipid Particles | 0.01 | 0.0099 | 0.0058 | 0.0047 | 0.0069 | 0.0049 | 0.0082 | 0.0057 | 0.0101 | 0.0064 | 0.0027 | 0.0072 | 0.0551 | 0.0226 | 0.0118 | 0.0024 | 0.0097 | 0.0088 |
Mitochondria | 0.0013 | 0.0014 | 0.0023 | 0.0021 | 0.001 | 0.0014 | 0.0008 | 0.0023 | 0.0011 | 0.0012 | 0.0031 | 0.0026 | 0.0104 | 0.0018 | 0.0012 | 0.0025 | 0.0103 | 0.0089 |
None | 0.0467 | 0.0135 | 0.0184 | 0.0688 | 0.0093 | 0.0224 | 0.0073 | 0.0131 | 0.0106 | 0.0056 | 0.0012 | 0.017 | 0.0154 | 0.0091 | 0.0028 | 0.006 | 0.0022 | 0.0022 |
Nuclear Periphery | 0.0057 | 0.0088 | 0.0076 | 0.0045 | 0.0043 | 0.0058 | 0.0039 | 0.0034 | 0.0054 | 0.0009 | 0.0025 | 0.0035 | 0.0048 | 0.0041 | 0.0035 | 0.0217 | 0.0347 | 0.0106 |
Nucleolus | 0.0011 | 0.0035 | 0.0044 | 0.0021 | 0.0009 | 0.0017 | 0.0003 | 0.0011 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0018 | 0.0052 | 0.0032 | 0.0007 | 0.0067 | 0.001 |
Nucleus | 0.015 | 0.014 | 0.019 | 0.0076 | 0.0089 | 0.0151 | 0.0082 | 0.0074 | 0.0047 | 0.0011 | 0.0016 | 0.0059 | 0.0073 | 0.0097 | 0.0166 | 0.0119 | 0.017 | 0.0171 |
Peroxisomes | 0.0104 | 0.0071 | 0.0095 | 0.007 | 0.0111 | 0.0075 | 0.0051 | 0.0053 | 0.0096 | 0.0074 | 0.0273 | 0.012 | 0.0146 | 0.0035 | 0.008 | 0.0023 | 0.0023 | 0.0037 |
Punctate Nuclear | 0.0125 | 0.0072 | 0.0132 | 0.0093 | 0.0202 | 0.0138 | 0.0076 | 0.0107 | 0.004 | 0.001 | 0.0349 | 0.0082 | 0.0134 | 0.0132 | 0.0056 | 0.0179 | 0.0075 | 0.0057 |
Vacuole | 0.0464 | 0.0584 | 0.0305 | 0.0366 | 0.0548 | 0.0458 | 0.052 | 0.05 | 0.026 | 0.0109 | 0.0296 | 0.0357 | 0.0783 | 0.1176 | 0.0755 | 0.108 | 0.0928 | 0.0923 |
Vacuole Periphery | 0.0063 | 0.0064 | 0.0053 | 0.0058 | 0.0096 | 0.0095 | 0.0064 | 0.0073 | 0.0054 | 0.0033 | 0.008 | 0.0121 | 0.0085 | 0.0113 | 0.0093 | 0.017 | 0.012 | 0.0179 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.7019 | 6.2232 | 8.4661 | 8.4433 | 12.0346 | 12.7558 | 10.7493 | 7.0781 | 10.1604 | 9.4995 |
Translational Efficiency | 2.8595 | 3.6219 | 1.9269 | 2.2133 | 2.138 | 1.8574 | 2.3377 | 3.405 | 1.9294 | 2.0252 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1743 | 2430 | 469 | 556 | 1685 | 2181 | 1341 | 1525 | 3428 | 4611 | 1810 | 2081 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 777.02 | 954.94 | 943.15 | 1089.32 | 839.84 | 873.22 | 987.17 | 1029.70 | 807.90 | 916.29 | 975.76 | 1045.63 |
Standard Deviation | 115.65 | 145.28 | 126.57 | 163.43 | 113.41 | 120.89 | 129.49 | 147.34 | 118.78 | 140.36 | 130.17 | 154.08 |
Intensity Change Log 2 | — | 0.297458 | 0.279536 | 0.487404 | — | 0.056231 | 0.233184 | 0.294038 | — | 0.177198 | 0.255645 | 0.390202 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000111 | 0.000098 | 0.000178 | 0.000274 | 0.000053 | 0.000181 | 0.000065 | 0.000186 | 0.000083 | 0.000137 | 0.000095 | 0.000209 |
Bud Neck | 0.009562 | 0.013328 | 0.033332 | 0.018210 | 0.009275 | 0.013429 | 0.021274 | 0.016549 | 0.009421 | 0.013376 | 0.024398 | 0.016993 |
Bud Site | 0.020012 | 0.020563 | 0.023478 | 0.037670 | 0.015922 | 0.017151 | 0.029118 | 0.027939 | 0.018002 | 0.018949 | 0.027656 | 0.030539 |
Cell Periphery | 0.000091 | 0.000051 | 0.000074 | 0.000057 | 0.000049 | 0.000052 | 0.000036 | 0.000068 | 0.000070 | 0.000051 | 0.000046 | 0.000065 |
Cytoplasm | 0.087733 | 0.057900 | 0.076492 | 0.052704 | 0.068548 | 0.033934 | 0.084106 | 0.042136 | 0.078303 | 0.046564 | 0.082133 | 0.044959 |
Cytoplasmic Foci | 0.156913 | 0.129463 | 0.037784 | 0.066319 | 0.097206 | 0.169518 | 0.072408 | 0.056360 | 0.127564 | 0.148409 | 0.063436 | 0.059021 |
Eisosomes | 0.000026 | 0.000010 | 0.000033 | 0.000011 | 0.000011 | 0.000015 | 0.000010 | 0.000013 | 0.000019 | 0.000012 | 0.000016 | 0.000012 |
Endoplasmic Reticulum | 0.001505 | 0.000973 | 0.002662 | 0.000384 | 0.000973 | 0.001036 | 0.000536 | 0.000827 | 0.001244 | 0.001003 | 0.001087 | 0.000708 |
Endosome | 0.289890* | 0.388649* | 0.220583* | 0.223421* | 0.422238* | 0.480900* | 0.281084* | 0.289744* | 0.354944* | 0.432283* | 0.265407* | 0.272023* |
Golgi | 0.017976 | 0.014816 | 0.002484 | 0.014384 | 0.011958 | 0.015687 | 0.005109 | 0.016358 | 0.015018 | 0.015228 | 0.004429 | 0.015831 |
Lipid Particles | 0.003351 | 0.001844 | 0.002125 | 0.001624 | 0.002436 | 0.003823 | 0.001391 | 0.001143 | 0.002901 | 0.002780 | 0.001581 | 0.001271 |
Mitochondria | 0.019399 | 0.006668 | 0.001340 | 0.003204 | 0.007066 | 0.007042 | 0.001479 | 0.003325 | 0.013337 | 0.006845 | 0.001443 | 0.003292 |
Mitotic Spindle | 0.005865 | 0.043607 | 0.034032 | 0.036415 | 0.004097 | 0.042318 | 0.043558 | 0.026541 | 0.004996 | 0.042998 | 0.041090 | 0.029179 |
None | 0.000986 | 0.000516 | 0.002056 | 0.000153 | 0.000310 | 0.000797 | 0.000320 | 0.000597 | 0.000654 | 0.000649 | 0.000770 | 0.000478 |
Nuclear Periphery | 0.000382 | 0.001242 | 0.002401 | 0.001251 | 0.000300 | 0.000639 | 0.001462 | 0.001298 | 0.000341 | 0.000956 | 0.001706 | 0.001286 |
Nuclear Periphery Foci | 0.000678 | 0.006817 | 0.008050 | 0.004484 | 0.001070 | 0.007640 | 0.002753 | 0.004134 | 0.000871 | 0.007206 | 0.004126 | 0.004227 |
Nucleolus | 0.001280 | 0.002891 | 0.023336 | 0.004221 | 0.000818 | 0.003933 | 0.006425 | 0.006000 | 0.001053 | 0.003384 | 0.010807 | 0.005525 |
Nucleus | 0.005769 | 0.003101 | 0.066547 | 0.006023 | 0.002180 | 0.003189 | 0.009931 | 0.005895 | 0.004005 | 0.003143 | 0.024601 | 0.005929 |
Peroxisomes | 0.003862 | 0.006379 | 0.005614 | 0.007646 | 0.004305 | 0.012481 | 0.007761 | 0.005451 | 0.004080 | 0.009265 | 0.007205 | 0.006037 |
Vacuole | 0.289616* | 0.246464* | 0.429144* | 0.481007* | 0.298758* | 0.155859 | 0.407270* | 0.449452* | 0.294110* | 0.203608* | 0.412938* | 0.457883* |
Vacuole Periphery | 0.084994 | 0.054620 | 0.028256 | 0.040538 | 0.052427 | 0.030378 | 0.023902 | 0.045986 | 0.068986 | 0.043153 | 0.025030 | 0.044530 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.29 | -3.11 | -1.11 | -1.17 | -0.13 | -1.09 | -3.04 | -2.70 | 0.01 | -1.86 | -0.90 | -2.81 | -2.41 | -0.78 | -1.48 |
Bud Neck | -3.69 | -9.27 | -7.04 | -5.17 | 3.19 | -3.46 | -11.00 | -6.39 | -3.33 | 5.25 | -5.04 | -14.49 | -9.30 | -5.66 | 6.10 |
Bud Site | -0.41 | -1.65 | -5.25 | -5.13 | -3.72 | -0.94 | -7.12 | -5.97 | -5.23 | 0.68 | -1.00 | -6.67 | -7.41 | -6.87 | -1.33 |
Cell Periphery | 1.89 | 0.84 | 1.64 | -0.86 | 1.83 | -0.33 | 2.15 | -1.52 | -1.18 | -2.59 | 1.70 | 2.02 | 0.65 | -1.42 | -1.91 |
Cytoplasm | 6.24 | -1.15 | 0.62 | -3.93 | 1.47 | 7.84 | -7.01 | 2.47 | -5.69 | 9.55 | 9.69 | -6.08 | 3.78 | -4.96 | 8.93 |
Cytoplasmic Foci | 5.01 | 25.89 | 19.01 | 15.37 | -4.39 | -14.56 | 8.91 | 11.84 | 24.86 | 3.01 | -6.24 | 22.57 | 23.34 | 29.39 | 0.82 |
Eisosomes | 3.35 | -1.56 | 3.03 | -1.46 | 9.43 | -3.05 | -0.93 | -1.20 | 2.02 | -0.44 | 2.66 | 0.39 | 2.65 | 0.05 | 6.22 |
Endoplasmic Reticulum | 2.35 | -5.37 | 9.79 | 2.38 | 9.68 | -0.14 | 1.09 | 0.52 | 0.61 | -0.58 | 1.10 | -0.28 | 2.73 | 1.23 | 3.57 |
Endosome | -11.06 | 9.28 | 11.51 | 21.04 | 0.68 | -5.54 | 21.33 | 16.78 | 23.62 | -5.05 | -11.05 | 20.29 | 16.49 | 27.82 | -4.09 |
Golgi | 2.69 | 15.52 | 4.14 | 2.53 | -4.94 | -3.00 | 8.71 | -1.41 | 0.73 | -7.20 | -0.03 | 16.60 | 1.37 | 1.46 | -8.52 |
Lipid Particles | 3.76 | 2.27 | 3.02 | -1.15 | 0.64 | -2.44 | 1.24 | 3.13 | 6.06 | 5.78 | 0.33 | 2.99 | 5.19 | 5.89 | 4.71 |
Mitochondria | 6.73 | 10.06 | 8.28 | 3.46 | -1.12 | 0.09 | 5.94 | 4.01 | 4.20 | -4.08 | 5.75 | 11.53 | 9.57 | 5.90 | -3.68 |
Mitotic Spindle | -13.46* | -4.99 | -7.33 | -0.04 | -1.26 | -13.38* | -12.32 | -8.58 | 3.71 | 5.40 | -18.99 | -13.15 | -11.07 | 3.45 | 4.09 |
None | 1.66 | -1.58 | 2.38 | 1.58 | 2.98 | -1.65 | -0.96 | -1.51 | 0.61 | -1.08 | -0.04 | -0.74 | 0.65 | 0.68 | 1.41 |
Nuclear Periphery | -4.69 | -9.23 | -7.48 | -2.35 | 4.30 | -4.29 | -7.29 | -4.76 | -3.44 | 1.39 | -5.93 | -10.58 | -6.50 | -3.10 | 2.86 |
Nuclear Periphery Foci | -6.86 | -6.31 | -5.89 | 1.97 | 2.55 | -6.59 | -5.44 | -3.12 | 3.11 | -0.35 | -9.49 | -8.84 | -4.93 | 3.62 | 0.91 |
Nucleolus | -4.49 | -8.32 | -5.63 | -3.01 | 6.53 | -5.84 | -9.33 | -6.03 | -2.42 | 0.98 | -7.40 | -12.05 | -7.17 | -3.60 | 4.77 |
Nucleus | 3.44 | -10.77 | -3.60 | -5.60 | 9.80 | -2.45 | -11.14 | -8.18 | -6.49 | 5.80 | 1.75 | -13.65 | -7.07 | -8.76 | 10.89 |
Peroxisomes | -4.52 | -1.94 | -3.05 | 0.12 | -1.70 | -9.44 | -3.10 | -1.30 | 8.00 | 1.80 | -10.17 | -3.94 | -2.71 | 6.00 | 1.12 |
Vacuole | 5.79 | -9.68 | -16.15 | -20.12 | -4.31 | 18.68 | -12.85 | -17.22 | -35.88 | -4.04 | 16.72 | -16.87 | -23.88 | -38.24 | -5.70 |
Vacuole Periphery | 8.57 | 16.42 | 14.18 | 7.67 | -2.58 | 9.09 | 15.39 | 4.05 | -4.98 | -11.33 | 11.79 | 23.91 | 12.80 | 2.02 | -11.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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