Standard name
Description Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.39 0.36 0.49 0.37 0.54 0.46 0.43 0.38 0.38 0.44 0.41 0.26 0.39 0.45 0.55 0.5 0.45 0.38 0.07 0.05 0.09 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.35 0.53 0.46 0.42 0.26 0.29 0.25 0.26 0.35 0.15 0.2 0.46 0.43 0.41 0.33 0.37 0.42 0.27 0.59 0.62 0.55 0.64 0.59
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.18 0.25 0.3 0.34 0.23 0.38 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.08 0 0 0.05 0 0.06 0 0 0 0.13 0.13 0.09 0.05 0 0.06 0 0 0.05 0.06 0.06 0.09
Vac/Vac Membrane 0.14 0.46 0.31 0.18 0.23 0.09 0.09 0.11 0.16 0.16 0.12 0.13 0.46 0.33 0.25 0.3 0.33 0.38 0.23 0.24 0.22 0.24 0.17 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 11 2 0 0 2 1 1 1 0 1 4 0 3 5 7 4 3 1 9 2 1 1 0
Bud 3 5 13 1 2 15 13 23 12 25 18 20 1 2 3 0 1 0 1 8 3 5 3 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 1 1
Bud Site 0 0 0 0 0 1 0 1 2 3 0 1 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 1 1 1 0 1 0 3 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 82 259 124 78 74 212 202 208 151 221 208 226 94 200 230 224 115 108 54 50 17 29 19 36
Endoplasmic Reticulum 2 15 7 1 6 11 7 6 5 1 8 10 3 0 2 14 7 6 1 3 0 0 4 7
Endosome 24 236 181 72 84 101 129 124 106 207 72 111 164 220 205 133 85 101 38 432 224 186 230 289
Golgi 0 3 0 0 1 0 1 3 0 0 2 0 1 0 1 8 3 1 1 5 1 0 0 5
Mitochondria 3 3 4 4 19 69 111 144 138 132 180 187 0 0 0 10 5 1 2 5 2 5 5 2
Nucleus 0 1 0 0 1 2 1 2 0 0 0 1 0 1 0 0 0 1 0 5 0 2 2 1
Nuclear Periphery 1 1 1 0 0 0 0 0 0 1 2 4 0 0 0 0 0 1 1 7 6 2 4 8
Nucleolus 3 17 18 4 2 8 12 19 9 21 20 10 16 18 26 11 8 7 0 2 0 1 0 1
Peroxisomes 0 0 1 4 0 0 5 1 6 2 1 3 3 0 0 0 1 0 0 0 0 0 0 1
SpindlePole 5 25 21 13 3 10 22 20 23 24 8 12 46 69 47 19 9 14 3 23 18 21 21 44
Vac/Vac Membrane 17 305 107 28 47 37 39 56 65 94 59 71 163 171 129 121 75 91 32 173 77 79 60 79
Unique Cell Count 124 668 344 158 201 391 439 487 401 585 473 546 356 514 506 406 230 242 144 734 360 339 361 493
Labelled Cell Count 142 881 479 205 239 469 544 609 518 732 579 663 491 684 649 547 313 334 144 734 360 339 361 493


Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 8.6 7.2 7.0 6.5 6.4 5.6 6.0 6.1 5.9 5.8 6.0 8.8 8.7 8.8 11.4 10.7 10.8 9.4 9.6 9.8
Std Deviation (1e-4) 2.4 1.1 1.0 1.2 1.2 1.0 0.9 1.0 1.3 1.1 1.1 1.1 1.1 1.1 1.0 2.0 2.1 1.7 1.3 1.4 1.5
Intensity Change (Log2) -0.04 -0.15 -0.18 -0.37 -0.26 -0.25 -0.29 -0.31 -0.28 0.28 0.27 0.28 0.65 0.57 0.58 0.38 0.4 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.9 0 -0.6 0.7 -0.6 0.4 0 -0.1 -3.3 -3.7 -3.4 -4.0 -2.5 -3.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 0.2 4.9 2.8 1.9 0.5 0.5 2.3 1.6 -2.8 0.8 2.7 5.2 3.3 2.1
Endoplasmic Reticulum 0 0 0.7 -0.5 -0.9 -0.9 0 -0.4 -0.2 0 0 0 1.2 0.8 0.4
Endosome -1.5 -2.4 -7.5 -6.6 -8.0 -7.3 -5.1 -11.4 -10.0 -1.7 -2.8 -3.5 -5.5 -3.7 -2.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.6 7.5 9.5 10.5 11.5 8.9 12.5 11.7 0 0 0 1.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.4 -2.5 -2.3 -1.8 -0.9 -2.2 -1.2 -0.7 -2.8 -0.5 -1.2 -0.1 -1.8 -1.0 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.9 -2.5 -2.4 -0.7 -1.3 -0.2 -1.4 -3.4 -3.0 3.1 3.4 1.7 -0.9 -1.2 -0.2
Vacuole -3.1 -1.9 -7.4 -7.9 -7.0 -4.8 -5.4 -6.5 -6.6 4.0 0.7 -1.8 -0.4 0.4 1.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vam7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available