Standard name | |
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Human Ortholog | |
Description | Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | 1 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 0 | 0 | 2 | 4 | 1 | 1 | 11 | – | 13 | 7 | 11 | 3 | 3 | 5 | 9 | 11 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 382 | 316 | 248 | 242 | 295 | 323 | 331 | 331 | – | 251 | 175 | 267 | 251 | 309 | 322 | 250 | 265 | 228 | 366 | 302 | 234 | 163 | 244 | 290 |
Endoplasmic Reticulum | 3 | 3 | 1 | 1 | 1 | 5 | 9 | 4 | – | 20 | 5 | 7 | 2 | 7 | 6 | 5 | 5 | 6 | 1 | 0 | 1 | 4 | 3 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1 | 7 | 21 | 57 | 55 | – | 47 | 67 | 68 | 0 | 0 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 3 | 1 | 3 |
Nucleus | 11 | 1 | 0 | 1 | 2 | 6 | 6 | 16 | – | 7 | 2 | 7 | 2 | 1 | 4 | 2 | 0 | 5 | 1 | 2 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 1 | 0 | 13 | 19 | 16 | 48 | 46 | – | 60 | 25 | 26 | 3 | 0 | 2 | 3 | 13 | 4 | 3 | 1 | 0 | 3 | 4 | 4 |
Unique Cell Count | 391 | 319 | 249 | 254 | 320 | 359 | 424 | 431 | 365 | 250 | 364 | 258 | 317 | 329 | 259 | 276 | 239 | 383 | 312 | 242 | 186 | 264 | 305 | |
Labelled Cell Count | 404 | 321 | 249 | 260 | 330 | 374 | 454 | 466 | 402 | 285 | 392 | 262 | 320 | 339 | 275 | 297 | 252 | 383 | 312 | 242 | 186 | 264 | 305 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.0 | 12.2 | 9.9 | 9.5 | 9.0 | 8.7 | 8.2 | 8.7 | – | 8.6 | 8.6 | 8.8 | 11.8 | 11.1 | 11.3 | 12.8 | 13.2 | 12.7 | 11.8 | 11.9 | 12.3 |
Std Deviation (1e-4) | 1.8 | 1.8 | 1.4 | 1.3 | 1.7 | 1.4 | 1.5 | 1.5 | – | 1.6 | 1.9 | 1.8 | 1.6 | 1.7 | 1.8 | 2.5 | 2.2 | 2.3 | 1.9 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.14 | -0.19 | -0.26 | -0.18 | – | -0.21 | -0.2 | -0.17 | 0.25 | 0.16 | 0.19 | 0.37 | 0.42 | 0.36 | 0.25 | 0.27 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 |
Cytoplasm | -3.1 | -4.2 | -4.9 | -7.8 | -8.1 | – | -9.6 | -9.2 | -8.7 | -2.1 | -2.0 | -1.8 | -2.5 | -2.7 | -3.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.9 | 6.0 | 5.9 | – | 5.9 | 8.8 | 7.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 3.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.6 | 3.9 | 3.4 | 5.5 | 5.3 | – | 6.7 | 5.1 | 4.3 | 0 | 0 | 0 | 0 | 3.5 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.2436 | 8.1562 | 7.7581 | 6.5507 | 6.298 | 7.3455 | 8.7565 | 10.9206 | 9.8641 | 8.7253 | 8.6963 | 9.6342 | 5.3492 | 7.9886 | 7.3147 | 7.8498 | 6.4301 | 7.04 |
Actin | 0.0197 | 0.0004 | 0.0079 | 0.003 | 0.0046 | 0.0086 | 0.0238 | 0.0005 | 0.0076 | 0.0114 | 0.0004 | 0.0024 | 0.0222 | 0.0002 | 0.0113 | 0.0003 | 0.0137 | 0.0031 |
Bud | 0.0011 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0039 | 0.0001 | 0 | 0.0001 | 0.037 | 0 | 0.0007 | 0.0009 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0002 |
Bud Neck | 0.0049 | 0.0002 | 0.0009 | 0.0016 | 0.0006 | 0.0015 | 0.0002 | 0.0001 | 0.0006 | 0.0006 | 0.0004 | 0.0008 | 0.0023 | 0.0001 | 0.0003 | 0.0005 | 0.0007 | 0.0012 |
Bud Periphery | 0.0006 | 0 | 0.0003 | 0.0003 | 0.0003 | 0.0018 | 0 | 0 | 0.0001 | 0.0185 | 0 | 0.0002 | 0.0008 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 |
Bud Site | 0.0029 | 0.0005 | 0.0028 | 0.003 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0007 | 0.0012 | 0.0001 | 0.0001 | 0.0191 | 0.0001 | 0.0003 | 0 | 0.0006 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.7864 | 0.9732 | 0.9044 | 0.8917 | 0.7579 | 0.8959 | 0.9267 | 0.9882 | 0.9422 | 0.8889 | 0.9538 | 0.9579 | 0.861 | 0.9793 | 0.9396 | 0.9915 | 0.7657 | 0.9388 |
Cytoplasmic Foci | 0.021 | 0.0029 | 0.0191 | 0.0152 | 0.028 | 0.0099 | 0.0021 | 0.0009 | 0.0121 | 0.0044 | 0.0033 | 0.0078 | 0.0289 | 0.0009 | 0.0023 | 0.0007 | 0.0142 | 0.0021 |
Eisosomes | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0138 | 0.0017 | 0.0014 | 0.0051 | 0.0025 | 0.0035 | 0.0019 | 0.0014 | 0.0014 | 0.0009 | 0.002 | 0.0023 | 0.0028 | 0.0004 | 0.0012 | 0.0006 | 0.0014 | 0.0023 |
Endosome | 0.0297 | 0.0012 | 0.0152 | 0.0091 | 0.044 | 0.017 | 0.0014 | 0.0003 | 0.0039 | 0.0006 | 0.0017 | 0.006 | 0.0061 | 0.0001 | 0.0149 | 0.0001 | 0.0335 | 0.0035 |
Golgi | 0.0056 | 0.0001 | 0.0066 | 0.0011 | 0.0134 | 0.006 | 0.0004 | 0.0001 | 0.0024 | 0.0002 | 0.0001 | 0.0008 | 0.0035 | 0 | 0.0057 | 0 | 0.0206 | 0.0002 |
Lipid Particles | 0.0099 | 0 | 0.0023 | 0.0056 | 0.0469 | 0.0021 | 0.0002 | 0 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0184 | 0 | 0.0007 | 0 | 0.0648 | 0.0001 |
Mitochondria | 0.0061 | 0.0002 | 0.003 | 0.0049 | 0.024 | 0.0083 | 0.0002 | 0 | 0.0004 | 0.0009 | 0.0001 | 0.0009 | 0.001 | 0 | 0.0103 | 0 | 0.0106 | 0.0011 |
None | 0.0178 | 0.0055 | 0.0046 | 0.0191 | 0.0127 | 0.0049 | 0.0104 | 0.0035 | 0.0106 | 0.0077 | 0.0302 | 0.003 | 0.0062 | 0.0158 | 0.0049 | 0.0011 | 0.0028 | 0.0024 |
Nuclear Periphery | 0.0229 | 0.0017 | 0.0017 | 0.0067 | 0.0075 | 0.0053 | 0.0021 | 0.0005 | 0.001 | 0.0022 | 0.0014 | 0.0043 | 0.0016 | 0.0002 | 0.0009 | 0.0004 | 0.0233 | 0.0118 |
Nucleolus | 0.0004 | 0 | 0.0001 | 0.0009 | 0.0005 | 0.0001 | 0 | 0 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0.0003 |
Nucleus | 0.0308 | 0.0108 | 0.0202 | 0.0214 | 0.0122 | 0.0245 | 0.0283 | 0.0041 | 0.0084 | 0.0217 | 0.0048 | 0.0101 | 0.0065 | 0.0025 | 0.0041 | 0.0043 | 0.0127 | 0.0285 |
Peroxisomes | 0.0154 | 0.0001 | 0.0051 | 0.0016 | 0.0199 | 0.0015 | 0.0007 | 0 | 0.0016 | 0.0005 | 0.0001 | 0.0003 | 0.0155 | 0 | 0.0004 | 0 | 0.015 | 0.0001 |
Punctate Nuclear | 0.0063 | 0.0006 | 0.0017 | 0.007 | 0.0196 | 0.0031 | 0.0008 | 0.0001 | 0.005 | 0.0026 | 0.001 | 0.0012 | 0.0017 | 0.0001 | 0.0003 | 0.0002 | 0.0171 | 0.003 |
Vacuole | 0.0027 | 0.0006 | 0.0015 | 0.0014 | 0.0019 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0005 | 0.0007 | 0.0001 | 0.0011 | 0.0001 | 0.0007 | 0.0005 |
Vacuole Periphery | 0.0016 | 0.0001 | 0.0005 | 0.0005 | 0.0023 | 0.0007 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0008 | 0 | 0.0015 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.9673 | 23.5905 | 18.5992 | 22.6712 | 36.3534 | 31.32 | 24.5977 | 25.1542 | 26.5232 | 34.1102 |
Translational Efficiency | 2.3821 | 1.1086 | 1.5073 | 1.2643 | 1.1071 | 1.6307 | 1.4437 | 1.6456 | 1.43 | 1.3725 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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