Standard name
Description Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p; essential for cell cycle progression from G2/M to G1 under nitrogen starvation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.07 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 – 0.1 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.97 0.61 0.56 0.62 0.71 – 0.66 0.67 0.71 0.7 0.92 0.89 0.83 0.94 0.95 0.95 0.86 0.89 0.9 0.75 0.74 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0
Endosome 0 0 0 0.22 0.2 0.28 0.16 – 0.16 0.09 0.09 0.1 0.06 0.1 0.14 0 0 0 0.06 0 0 0 0.05 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.08 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.17 0.19 0.09 0.06 – 0.06 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0.15 0.13 – 0.12 0.14 0.12 0.14 0 0 0 0 0 0.05 0 0 0 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 3 14 12 5 18 – 6 5 3 7 4 3 5 1 0 0 2 2 0 3 3 5
Bud 1 2 2 6 10 8 25 – 32 19 19 12 18 9 5 0 0 1 1 0 0 4 8 12
Bud Neck 1 0 0 0 2 0 0 – 1 1 2 1 0 0 0 0 0 0 1 2 0 0 2 5
Bud Site 0 0 0 0 1 2 0 – 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 3 2 2 3 4 5 – 5 3 3 1 1 1 1 6 7 4 0 0 0 0 0 0
Cytoplasm 306 424 286 122 169 227 330 – 219 228 183 164 369 411 444 325 246 219 284 408 267 311 427 481
Endoplasmic Reticulum 6 6 3 6 3 2 4 – 1 2 2 6 2 0 12 6 10 6 0 4 0 30 39 25
Endosome 8 9 1 44 60 101 74 – 52 30 24 24 23 48 76 5 1 4 19 18 8 12 31 29
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 1 3 0 2 2 4
Mitochondria 2 3 0 1 2 3 9 – 27 38 32 8 2 0 1 4 0 2 3 2 6 13 13 10
Nucleus 3 1 1 0 0 2 5 – 2 5 2 0 0 0 0 2 2 3 2 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 0 0 0 2 1
Nucleolus 0 0 0 1 3 8 2 – 6 2 3 1 1 1 2 0 0 0 1 0 0 0 1 0
Peroxisomes 0 0 0 1 0 1 0 – 1 0 1 1 2 6 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 35 58 32 29 – 20 22 13 14 0 0 6 4 1 0 3 1 0 2 2 7
Vac/Vac Membrane 1 2 3 3 29 56 59 – 40 48 30 34 1 8 6 10 11 11 3 10 7 25 34 31
Unique Cell Count 320 444 296 200 304 364 463 332 340 258 235 402 464 537 346 258 230 331 457 297 417 575 623
Labelled Cell Count 334 453 302 235 352 451 560 413 404 318 273 423 487 561 364 278 250 331 457 297 417 575 623


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 7.9 7.1 7.0 8.1 6.5 6.3 – 5.3 4.9 4.7 5.6 6.3 6.9 7.2 13.7 15.0 12.5 5.9 6.4 7.0
Std Deviation (1e-4) 1.0 1.2 1.1 1.6 1.5 1.4 1.1 – 1.1 1.0 0.9 1.4 1.2 1.4 1.2 3.1 2.9 2.8 1.4 1.4 1.7
Intensity Change (Log2) – – – -0.02 0.18 -0.12 -0.18 – -0.43 -0.54 -0.59 -0.34 -0.18 -0.04 0.02 0.95 1.08 0.82 -0.27 -0.14 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.6 2.3 0 2.4 – 0 0 0 0 0 0 0 0 0 0
Bud 0 2.3 0 3.4 – 5.0 0 0 3.2 3.0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -10.2 -11.7 -10.5 -8.7 – -9.7 -9.4 -8.4 -8.5 -2.6 -3.9 -5.8 -1.6 -0.8 -0.8
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 1.3 0 2.2 0
Endosome 8.2 7.9 9.7 7.0 – 6.9 0 0 5.3 3.9 5.5 6.6 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 5.0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 7.2 7.7 5.0 4.1 – 4.0 0 0 3.9 0 0 0 0 0 0
Vacuole 0 4.6 6.4 5.8 – 5.5 6.1 5.3 6.0 0 0 0 1.7 2.4 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8588 5.1824 4.4671 4.4732 3.2326 4.9136 1.9433 3.5697 2.7612 2.7549 2.5493 3.1229 1.9386 3.3603 2.6073 2.8428 2.2969 2.9061
Actin 0.0705 0.0002 0.0147 0.0054 0.0001 0.001 0.0338 0.0092 0.0027 0.0028 0.0166 0.0006 0.0043 0.0036 0.0048 0.0008 0.0017 0.002
Bud 0.0006 0.0002 0.0003 0.0007 0.0001 0.0001 0.0007 0.0051 0.0027 0.0019 0.0012 0.0006 0.0102 0.0352 0.0233 0.0004 0.0681 0.0079
Bud Neck 0.0039 0.0001 0.0004 0.0009 0.0001 0.0017 0.0163 0.0003 0.0031 0.0004 0.0011 0.0007 0.0013 0.0002 0.0006 0.0003 0.0009 0.0011
Bud Periphery 0.0012 0.0001 0.0003 0.0006 0 0.0001 0.0007 0.0017 0.0035 0.005 0.0008 0.0004 0.0066 0.0109 0.0091 0.0002 0.0264 0.0047
Bud Site 0.002 0.0006 0.001 0.0044 0.0001 0.0001 0.0145 0.0079 0.0059 0.0034 0.0011 0.0005 0.0064 0.0171 0.0304 0.0014 0.0047 0.0004
Cell Periphery 0.0005 0.0003 0.0003 0.0002 0.0001 0.0001 0.0003 0.0002 0.0003 0.0004 0.0002 0.0001 0.0006 0.0007 0.0007 0.0005 0.0006 0.0002
Cytoplasm 0.6007 0.8445 0.7336 0.7341 0.7559 0.8118 0.5963 0.8546 0.8209 0.8604 0.5755 0.8259 0.6247 0.7827 0.7089 0.7238 0.659 0.7961
Cytoplasmic Foci 0.0184 0.0289 0.0447 0.0275 0.0349 0.021 0.0665 0.0502 0.0346 0.0177 0.0969 0.0551 0.0414 0.0319 0.0695 0.0506 0.0622 0.0351
Eisosomes 0.001 0 0.0004 0.0002 0 0 0.0002 0 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0.0012 0 0
Endoplasmic Reticulum 0.012 0.0031 0.0036 0.0043 0.0019 0.0027 0.0054 0.0017 0.0034 0.0013 0.0032 0.0035 0.0081 0.002 0.0039 0.0046 0.0031 0.0032
Endosome 0.0391 0.0269 0.0442 0.0748 0.0385 0.0338 0.0729 0.0292 0.0528 0.0226 0.118 0.0553 0.0465 0.0227 0.0449 0.0947 0.0475 0.0272
Golgi 0.005 0.0009 0.0021 0.0017 0.0013 0.0008 0.012 0.0021 0.0058 0.0038 0.0306 0.0036 0.0017 0.0011 0.0019 0.0014 0.0007 0.0006
Lipid Particles 0.0042 0.0005 0.0022 0.0008 0.0001 0.0004 0.0195 0.0001 0.004 0.0017 0.047 0.0003 0.0019 0.0002 0.0007 0.0022 0.0005 0.0003
Mitochondria 0.0021 0.0001 0.001 0.0031 0.0003 0.0004 0.0126 0.0004 0.0094 0.0495 0.0388 0.0024 0.0017 0.0042 0.0043 0.005 0.0016 0.0008
None 0.2067 0.0875 0.1284 0.1072 0.1639 0.1144 0.0752 0.03 0.0329 0.0147 0.0281 0.0262 0.2266 0.0758 0.0781 0.0727 0.0988 0.1077
Nuclear Periphery 0.0119 0.0006 0.0015 0.004 0.0002 0.0026 0.0201 0.0004 0.0011 0.0006 0.0075 0.0072 0.0027 0.0007 0.0021 0.0023 0.0017 0.0021
Nucleolus 0.0006 0 0.0002 0.0009 0 0.0002 0.0038 0.0001 0.0004 0.0003 0.0008 0.0005 0.0004 0.0007 0.001 0.0161 0.0006 0.0003
Nucleus 0.0038 0.0008 0.0016 0.006 0.0004 0.0018 0.0162 0.0012 0.0017 0.0011 0.0019 0.0065 0.0033 0.0023 0.0035 0.0042 0.0037 0.0026
Peroxisomes 0.0024 0.0001 0.0049 0.0006 0.0002 0.0002 0.0085 0.0008 0.0027 0.0013 0.0073 0.0012 0.0005 0.0005 0.0007 0.0006 0.0005 0.0003
Punctate Nuclear 0.0061 0.0003 0.0028 0.002 0.0001 0.0017 0.0153 0.0006 0.0007 0.0004 0.0116 0.0024 0.0035 0.001 0.0032 0.0024 0.0038 0.0019
Vacuole 0.0056 0.0038 0.0105 0.0174 0.0015 0.0043 0.0072 0.0037 0.0095 0.0084 0.0078 0.0053 0.0067 0.0056 0.007 0.0122 0.0121 0.0045
Vacuole Periphery 0.0016 0.0004 0.0011 0.0032 0.0004 0.0008 0.0021 0.0005 0.0015 0.0023 0.004 0.0018 0.0008 0.0007 0.0013 0.0024 0.0019 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.9389 3.6614 8.1507 11.0391 4.7793 8.187 13.9751 15.8662 14.6729 11.0618
Translational Efficiency 0.6391 1.2717 0.7452 0.6315 0.7743 0.7762 0.553 0.4591 0.6091 0.5935

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘