Standard name
Description Ornithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.89 0.93 0.97 0.98 0.88 0.87 0.78 0.82 0.57 0.72 0.99 0.99 0.99 0.98 0.96 0.96 0.97 0.94 0.83 0.94 0.96 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.11 0.1 0.11 0.27 0.24 0.42 0.4 0.65 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 5
Bud 1 1 1 1 2 0 2 1 1 2 3 5 1 0 0 0 0 1 0 0 0 3 2 15
Bud Neck 0 0 0 0 0 1 0 7 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cell Periphery 0 1 0 0 1 0 0 1 0 4 1 1 4 1 1 0 2 4 0 0 0 0 0 2
Cytoplasm 168 100 58 93 102 133 164 232 139 146 79 126 519 709 707 108 124 77 184 113 61 313 394 389
Endoplasmic Reticulum 0 0 1 1 0 0 0 1 0 0 1 0 4 3 2 6 14 2 1 0 0 2 1 4
Endosome 0 0 0 0 0 0 0 2 0 0 0 0 0 2 0 1 1 1 0 1 0 1 1 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 1
Mitochondria 5 1 8 11 10 15 51 63 76 71 91 84 0 1 0 2 2 1 0 0 1 3 0 6
Nucleus 1 0 0 0 0 1 4 3 2 2 0 2 1 1 0 0 1 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
Nucleolus 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 3 4 2 2 3 0 0 0 0 0 0 0 0 0 0 0 6
Vac/Vac Membrane 1 1 1 0 0 0 1 2 0 1 0 0 2 2 11 0 2 1 1 1 0 1 0 6
Unique Cell Count 172 102 65 100 105 136 187 268 179 179 139 175 525 716 716 110 129 80 190 121 74 333 409 455
Labelled Cell Count 176 104 69 108 115 151 224 315 224 229 177 223 531 719 722 117 148 87 190 121 74 333 409 455


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.8 4.2 4.4 3.8 3.2 3.0 3.2 2.8 2.6 2.9 2.7 10.2 11.5 11.8 5.1 6.2 5.8 5.5 6.1 6.0
Std Deviation (1e-4) 0.5 1.1 1.2 0.8 0.9 1.4 1.6 1.6 1.2 1.2 1.6 1.6 2.2 2.4 2.3 1.2 1.4 0.8 1.0 1.4 1.3
Intensity Change (Log2) 0.04 -0.15 -0.39 -0.51 -0.41 -0.58 -0.68 -0.54 -0.65 1.26 1.44 1.47 0.26 0.54 0.46 0.38 0.52 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 2.1 2.6 -0.3 -0.6 -2.0 -1.4 -4.6 -2.8 5.0 5.7 5.2 2.6 1.9 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spe1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available