Standard name
Human Ortholog
Description Ornithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.89 0.93 0.97 0.98 0.88 0.87 0.78 0.82 0.57 0.72 0.99 0.99 0.99 0.98 0.96 0.96 0.97 0.94 0.83 0.94 0.96 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.11 0.1 0.11 0.27 0.24 0.42 0.4 0.65 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 5
Bud 1 1 1 1 2 0 2 1 1 2 3 5 1 0 0 0 0 1 0 0 0 3 2 15
Bud Neck 0 0 0 0 0 1 0 7 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 1 0 0 1 0 4 1 1 4 1 1 0 2 4 0 0 0 0 0 2
Cytoplasm 168 100 58 93 102 133 164 232 139 146 79 126 519 709 707 108 124 77 184 113 61 313 394 389
Endoplasmic Reticulum 0 0 1 1 0 0 0 1 0 0 1 0 4 3 2 6 14 2 1 0 0 2 1 4
Endosome 0 0 0 0 0 0 0 2 0 0 0 0 0 2 0 1 1 1 0 1 0 1 1 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 1
Mitochondria 5 1 8 11 10 15 51 63 76 71 91 84 0 1 0 2 2 1 0 0 1 3 0 6
Nucleus 1 0 0 0 0 1 4 3 2 2 0 2 1 1 0 0 1 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
Nucleolus 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 3 4 2 2 3 0 0 0 0 0 0 0 0 0 0 0 6
Vac/Vac Membrane 1 1 1 0 0 0 1 2 0 1 0 0 2 2 11 0 2 1 1 1 0 1 0 6
Unique Cell Count 172 102 65 100 105 136 187 268 179 179 139 175 525 716 716 110 129 80 190 121 74 333 409 455
Labelled Cell Count 176 104 69 108 115 151 224 315 224 229 177 223 531 719 722 117 148 87 190 121 74 333 409 455


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.8 4.2 4.4 3.8 3.2 3.0 3.2 2.8 2.6 2.9 2.7 10.2 11.5 11.8 5.1 6.2 5.8 5.5 6.1 6.0
Std Deviation (1e-4) 0.5 1.1 1.2 0.8 0.9 1.4 1.6 1.6 1.2 1.2 1.6 1.6 2.2 2.4 2.3 1.2 1.4 0.8 1.0 1.4 1.3
Intensity Change (Log2) – – – 0.04 -0.15 -0.39 -0.51 -0.41 -0.58 -0.68 -0.54 -0.65 1.26 1.44 1.47 0.26 0.54 0.46 0.38 0.52 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 2.1 2.6 -0.3 -0.6 -2.0 -1.4 -4.6 -2.8 5.0 5.7 5.2 2.6 1.9 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0164 5.4353 5.2323 4.9921 5.3177 5.4589 1.8272 1.6628 3.4125 3.621 2.6786 3.0389 3.4048 3.5983 3.2164 3.7885 3.1269 3.4007
Actin 0.0866 0.0003 0.0149 0.0131 0.0002 0.0111 0.2266 0.0267 0.0003 0.0012 0.0002 0.0058 0.0481 0.0018 0.0102 0.0106 0.0126 0.0015
Bud 0.0008 0.0035 0.0092 0.0017 0.0001 0.0012 0.0013 0.0004 0.0001 0.0002 0 0.0001 0.0014 0.0007 0.0007 0.002 0.001 0.0001
Bud Neck 0.0025 0.0001 0.0013 0.0007 0.0002 0.0029 0.0006 0.0002 0 0.0001 0.0001 0.0007 0.0003 0.0003 0.0041 0.0002 0.001 0.0004
Bud Periphery 0.0011 0.0008 0.0026 0.0011 0.0001 0.0015 0.0035 0.0002 0 0.0001 0 0.0001 0.0014 0.0006 0.0009 0.002 0.0025 0.0001
Bud Site 0.0033 0.0053 0.0031 0.0018 0.0001 0.0008 0.0013 0.0012 0.0003 0.0003 0 0.0012 0.0016 0.0067 0.0089 0.0006 0.0082 0
Cell Periphery 0.0004 0.0002 0.0002 0.0001 0 0.0002 0.0005 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0002 0.0002 0.0001 0.0005 0
Cytoplasm 0.4843 0.817 0.7349 0.5248 0.7103 0.7868 0.0134 0.5829 0.3445 0.4994 0.7283 0.6414 0.4583 0.64 0.6242 0.4728 0.5209 0.6221
Cytoplasmic Foci 0.0169 0.0045 0.0144 0.0362 0.008 0.0093 0.0108 0.0183 0.0053 0.0358 0.0019 0.0277 0.016 0.0086 0.0065 0.0325 0.0192 0.0077
Eisosomes 0.0005 0 0.0016 0.0002 0.0001 0.0001 0.0067 0.0001 0.0001 0.0001 0 0.0003 0.0004 0.0001 0.0001 0.0001 0.0008 0
Endoplasmic Reticulum 0.0183 0.0018 0.0038 0.0033 0.0027 0.0067 0.0077 0.0029 0.0029 0.0024 0.0012 0.0012 0.0075 0.0049 0.0022 0.006 0.0021 0.0023
Endosome 0.0251 0.0022 0.008 0.0021 0.0038 0.0107 0.0302 0.0031 0.0016 0.1549 0.0004 0.0031 0.0191 0.0015 0.0078 0.0055 0.012 0.0018
Golgi 0.0082 0.0002 0.0026 0.0014 0.0001 0.0015 0.0309 0.0013 0.0002 0.0428 0 0.0019 0.0026 0.0004 0.0059 0.001 0.0033 0.0002
Lipid Particles 0.0076 0.0001 0.0058 0.0085 0.0002 0.0007 0.0478 0.0002 0.0008 0.0044 0 0.0064 0.0037 0.0006 0.0004 0.001 0.0312 0.0002
Mitochondria 0.0026 0.0002 0.0012 0.0013 0.0001 0.0161 0.0236 0.0005 0.0001 0.003 0.0001 0.0021 0.0013 0.0002 0.0014 0.0005 0.052 0.0001
None 0.2898 0.1599 0.17 0.2973 0.2704 0.1369 0.5359 0.3547 0.6407 0.2494 0.2644 0.2947 0.414 0.3256 0.3226 0.4481 0.3079 0.3604
Nuclear Periphery 0.024 0.0005 0.0043 0.0015 0.0007 0.0035 0.0153 0.0013 0.0007 0.0008 0.0004 0.0002 0.0064 0.0012 0.0005 0.0024 0.0005 0.0005
Nucleolus 0.0005 0.0001 0.0004 0.0002 0 0.0002 0.0009 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0002 0.0001 0.0001 0.0054 0
Nucleus 0.0066 0.0014 0.0051 0.0016 0.0011 0.0039 0.0012 0.0014 0.0008 0.0005 0.0024 0.0004 0.0032 0.0017 0.0009 0.0017 0.0012 0.0011
Peroxisomes 0.0058 0 0.0076 0.0948 0.0001 0.0007 0.0311 0.0022 0.0001 0.0013 0 0.0115 0.002 0.0003 0.0012 0.0038 0.0087 0.0002
Punctate Nuclear 0.0119 0.0004 0.0076 0.0075 0.0004 0.0012 0.005 0.0013 0.0005 0.0004 0.0003 0.0005 0.0105 0.0035 0.0003 0.0077 0.0066 0.0008
Vacuole 0.002 0.0015 0.001 0.0007 0.001 0.003 0.0014 0.0008 0.0008 0.0015 0.0002 0.0004 0.0012 0.0007 0.0007 0.0009 0.002 0.0003
Vacuole Periphery 0.0013 0.0001 0.0003 0.0002 0.0002 0.001 0.0043 0.0002 0.0001 0.0013 0 0.0001 0.0005 0.0001 0.0002 0.0003 0.0005 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 113.0182 117.5766 117.5482 106.03 103.1348 104.6906 120.1273 123.4786 111.3538 108.1132
Translational Efficiency 1.1651 1.1141 1.0 1.0589 0.8406 1.4049 1.1178 0.9618 1.0423 0.9284

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ornithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Spe1

Spe1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spe1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available