Standard name
Description Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.96 0.96 0.32 0.31 0.37 0.4 0.44 0.49 0.47 0.95 0.96 0.95 0.61 0.82 0.84 0.65 0.71 0.75 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.38 0.3 0.24 0.14 0.1 0.09 0.1 0 0 0 0.15 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.08 0 0.07 0 0.07 0.11 0.08 0 0 0 0 0 0 0 0.13 0.12 0.1 0.07
Nucleus 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.13 0.13 0.09 0.06 0.07 0.05 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.41 0.23 0.17 0.1 0.06 0.1 0.06 0 0 0 0.16 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0.1 0.26 0.38 0.41 0.42 0.38 0.49 0 0 0 0.27 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 1 0 1 1 2 1 1 2 4 6 5 6 4
Bud 1 3 1 4 14 22 20 22 9 13 14 0 0 0 4 7 4 3 5 7 12
Bud Neck 2 0 1 0 4 1 0 0 1 0 0 2 1 0 0 1 2 2 0 4 3
Bud Site 0 0 1 0 2 5 2 3 3 3 2 0 0 0 2
Cell Periphery 2 2 1 0 1 0 0 0 1 2 0 0 0 0 0 2 0 2 3 2 2
Cytoplasm 394 318 322 235 87 124 201 216 215 168 263 220 220 205 230 336 283 216 186 255 223
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 1 0 0 6 6 3 10 0 0 5 1 2 1
Endosome 1 2 4 4 102 121 129 73 48 30 54 2 2 2 58 10 5 14 4 5 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 2 0 0 2
Mitochondria 7 18 13 20 4 29 18 35 55 26 18 0 0 0 13 11 12 44 32 32 22
Nucleus 0 0 0 0 2 7 8 29 29 19 7 2 2 2 4 0 1 0 1 0 0
Nuclear Periphery 0 0 2 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 1 0 12 50 69 47 29 23 28 0 0 0 18 0 0 0 0 0 0
Peroxisomes 1 0 2 0 29 14 6 3 4 4 4 0 0 0 5 0 0 0 0 0 0
SpindlePole 7 1 8 5 110 93 92 53 31 33 34 2 1 4 61 14 6 14 5 6 14
Vac/Vac Membrane 1 2 5 0 26 102 205 221 201 128 272 0 0 0 101 9 7 15 7 7 12
Unique Cell Count 410 331 336 246 269 398 543 537 484 340 559 232 230 215 377 408 338 335 262 339 312
Labelled Cell Count 417 347 361 268 393 569 750 702 629 450 699 235 234 217 508 408 338 335 262 339 312


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.7 3.5 3.3 4.8 5.4 6.1 6.2 6.1 6.5 7.5 5.1 5.1 5.2 4.7 4.7 6.6 5.0 5.1 5.6
Std Deviation (1e-4) 0.6 0.9 1.6 1.3 1.8 1.7 1.9 2.0 2.1 2.4 2.9 1.2 1.2 1.1 1.7 1.2 2.8 1.9 1.6 2.2
Intensity Change (Log2) -0.08 0.46 0.63 0.8 0.83 0.79 0.89 1.1 0.55 0.55 0.58 0.44 0.42 0.93 0.52 0.54 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 9.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ams1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available