Standard name
Human Ortholog
Description Beta subunit of geranylgeranyltransferase type I; subunit of the Ram2p-Cdc43p heterodimer that catalyzes the geranylgeranylation of the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.98 0.97 0.93 0.89 0.81 0.83 0.73 0.79 0.67 0.75 0.95 0.97 0.98 0.93 0.93 0.93 0.95 0.94 0.95 0.93 0.93 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.18 0.27 0.23 0.41 0.25 0.53 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.05 0 0.05 0 0 0.06 0.09 0.07 0.05 0.09 0.08 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 0 1 1 2 5
Bud 1 1 0 0 1 0 3 6 10 7 10 9 0 0 0 0 0 0 2 0 1 6 8 20
Bud Neck 0 0 1 0 1 0 1 0 0 5 0 0 0 1 0 0 0 0 0 0 0 0 2 5
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 2 3 4 4 3 2 3 0 3 1 1 0 6 7 5 0 0 0 0 0 1
Cytoplasm 449 264 320 331 430 395 392 446 323 400 292 276 341 425 485 206 166 177 486 255 323 354 503 532
Endoplasmic Reticulum 1 1 4 0 1 0 0 2 1 1 1 0 0 0 0 5 6 3 0 0 1 1 3 8
Endosome 3 0 0 0 2 0 0 1 0 0 0 0 0 1 1 2 1 2 3 2 0 2 2 1
Golgi 1 0 0 0 2 0 0 0 0 0 0 0 0 1 0 2 1 2 1 0 0 1 0 2
Mitochondria 3 1 1 6 24 80 130 122 182 127 232 122 0 1 0 4 4 2 2 4 5 2 5 5
Nucleus 3 1 0 1 0 3 5 13 7 10 8 5 0 0 2 3 3 3 3 2 0 0 1 0
Nuclear Periphery 2 0 0 1 1 1 0 1 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 2 3 1 1 0 0 0 0 0 3 0 1 1 3 9
Vac/Vac Membrane 15 5 3 12 29 20 26 15 20 20 10 22 33 30 23 19 14 13 2 1 2 2 1 3
Unique Cell Count 467 268 326 340 463 446 486 537 440 508 435 368 360 440 496 222 179 191 515 272 342 382 544 603
Labelled Cell Count 481 274 331 353 494 503 562 611 546 577 558 439 376 460 512 247 202 207 515 272 342 382 544 603


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.0 6.0 5.7 5.6 4.5 4.2 4.8 3.9 4.2 3.8 4.2 6.3 6.9 6.8 7.0 7.8 7.2 6.6 6.7 7.3
Std Deviation (1e-4) 0.9 1.2 0.8 1.5 1.4 1.1 1.2 1.2 1.2 1.2 1.0 1.2 1.1 1.9 1.5 1.5 1.5 1.7 1.8 1.7 2.2
Intensity Change (Log2) – – – -0.07 -0.12 -0.44 -0.53 -0.34 -0.63 -0.51 -0.68 -0.52 0.05 0.19 0.16 0.22 0.37 0.26 0.13 0.15 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -3.4 -5.0 -7.4 -6.8 -9.2 -8.0 -10.7 -8.7 -2.4 -1.3 -0.4 -3.1 -3.1 -3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.3 3.7 2.9 3.3 1.9 2.9 2.6 1.5 3.6 4.8 4.0 3.0 4.5 4.1 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9516 2.6256 2.3886 2.0349 2.2912 2.33 0.8695 1.1909 1.074 0.5733 0.2847 1.454 0.8895 1.4125 1.2816 1.2603 1.1261 1.3569
Actin 0.0148 0.0045 0.0127 0.0012 0.0264 0.004 0.0066 0.0003 0.0014 0.0165 0.0015 0.0082 0.0098 0.0002 0.0007 0.0001 0.0032 0.0007
Bud 0.0008 0.0025 0.006 0.0031 0.0031 0.0004 0.0031 0.0087 0.0053 0.0004 0.0009 0.0035 0.0004 0.0002 0.0003 0.0002 0.0003 0.0004
Bud Neck 0.0041 0.0009 0.003 0.0004 0.0135 0.001 0.0019 0.0002 0.0009 0.0004 0.0201 0.0016 0.0033 0.0002 0.0003 0.0006 0.0016 0.0023
Bud Periphery 0.0011 0.0016 0.0048 0.0157 0.0056 0.0004 0.0018 0.0019 0.0012 0.0008 0.0009 0.0019 0.0002 0.0001 0.0002 0.0001 0.0002 0.0005
Bud Site 0.0107 0.0102 0.0071 0.0008 0.0173 0.0003 0.0065 0.0067 0.0082 0.0002 0.0137 0.0006 0.0066 0.0019 0.0007 0.0002 0.0008 0.0002
Cell Periphery 0.0003 0.0002 0.0005 0.0001 0.0003 0.0001 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0 0 0.0001 0
Cytoplasm 0.4268 0.6582 0.4928 0.5378 0.507 0.5806 0.3752 0.5945 0.453 0.5328 0.4933 0.5607 0.6628 0.7043 0.6708 0.7699 0.8057 0.7518
Cytoplasmic Foci 0.0211 0.0142 0.0117 0.0101 0.0411 0.0166 0.0508 0.0092 0.0211 0.0079 0.0138 0.0179 0.0105 0.0087 0.0082 0.0051 0.0163 0.0104
Eisosomes 0.0002 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0039 0.0028 0.0024 0.0024 0.0041 0.0022 0.0037 0.0016 0.0018 0.0007 0.0009 0.0019 0.0033 0.0024 0.0021 0.0014 0.0032 0.0021
Endosome 0.0201 0.0067 0.0072 0.0111 0.0198 0.0066 0.0233 0.0014 0.0144 0.0017 0.0017 0.0136 0.006 0.0027 0.003 0.0023 0.0094 0.0025
Golgi 0.0068 0.0014 0.0026 0.0004 0.0081 0.0012 0.0042 0.0001 0.0023 0.0003 0.0015 0.0024 0.0006 0.0001 0.0002 0.0001 0.0013 0.0001
Lipid Particles 0.0089 0.0005 0.0078 0.0003 0.0093 0.0014 0.0063 0.0002 0.004 0.0001 0.0005 0.0059 0.0005 0.0002 0.0001 0 0.0014 0.0001
Mitochondria 0.0054 0.0024 0.0098 0.0022 0.0141 0.0017 0.0045 0.0009 0.0016 0.0007 0.0008 0.0012 0.0003 0.0002 0.0004 0.0004 0.0005 0.0003
None 0.4448 0.2525 0.3909 0.3706 0.2724 0.3502 0.4496 0.3611 0.4527 0.3987 0.4374 0.3411 0.2791 0.264 0.2816 0.1679 0.1157 0.1881
Nuclear Periphery 0.007 0.003 0.0035 0.0091 0.0053 0.0051 0.0113 0.0009 0.0023 0.0009 0.0008 0.0028 0.0019 0.0013 0.0016 0.0018 0.0026 0.0019
Nucleolus 0.0013 0.0009 0.001 0.0005 0.0009 0.0003 0.0017 0.0007 0.001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0002 0.0002 0.0003 0.0002
Nucleus 0.0068 0.0167 0.0161 0.0246 0.013 0.0188 0.0221 0.0081 0.0126 0.0105 0.0038 0.0165 0.0095 0.0081 0.0245 0.0463 0.0243 0.031
Peroxisomes 0.0065 0.0019 0.0047 0.0009 0.0194 0.0014 0.013 0.0001 0.0063 0.0247 0.006 0.0133 0.0004 0.0002 0.0004 0.0001 0.001 0.0005
Punctate Nuclear 0.0056 0.0168 0.0127 0.0051 0.0163 0.0064 0.0089 0.0018 0.0063 0.0017 0.0012 0.0049 0.003 0.0038 0.0032 0.0017 0.0106 0.0058
Vacuole 0.0023 0.0017 0.0017 0.0022 0.0021 0.0008 0.0041 0.0012 0.003 0.0007 0.0006 0.0012 0.0012 0.0011 0.0012 0.0015 0.0012 0.0009
Vacuole Periphery 0.0008 0.0003 0.0006 0.0012 0.0008 0.0004 0.001 0.0002 0.0005 0.0001 0.0001 0.0004 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.39 27.9081 23.9448 25.988 34.1525 18.5262 29.5405 21.1282 24.2322 30.9132
Translational Efficiency 0.707 0.5943 0.611 0.5733 0.3957 0.7977 0.5221 0.6857 0.64 0.48

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Beta subunit of geranylgeranyltransferase type I; subunit of the Ram2p-Cdc43p heterodimer that catalyzes the geranylgeranylation of the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
Localization
Cell Percentages cytoplasm (60%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cdc43

Cdc43


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc43-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available