Standard name
Description Beta subunit of geranylgeranyltransferase type I; subunit of the Ram2p-Cdc43p heterodimer that catalyzes the geranylgeranylation of the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.98 0.97 0.93 0.89 0.81 0.83 0.73 0.79 0.67 0.75 0.95 0.97 0.98 0.93 0.93 0.93 0.95 0.94 0.95 0.93 0.93 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.18 0.27 0.23 0.41 0.25 0.53 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.05 0 0.05 0 0 0.06 0.09 0.07 0.05 0.09 0.08 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 0 1 1 2 5
Bud 1 1 0 0 1 0 3 6 10 7 10 9 0 0 0 0 0 0 2 0 1 6 8 20
Bud Neck 0 0 1 0 1 0 1 0 0 5 0 0 0 1 0 0 0 0 0 0 0 0 2 5
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cell Periphery 1 1 2 2 3 4 4 3 2 3 0 3 1 1 0 6 7 5 0 0 0 0 0 1
Cytoplasm 449 264 320 331 430 395 392 446 323 400 292 276 341 425 485 206 166 177 486 255 323 354 503 532
Endoplasmic Reticulum 1 1 4 0 1 0 0 2 1 1 1 0 0 0 0 5 6 3 0 0 1 1 3 8
Endosome 3 0 0 0 2 0 0 1 0 0 0 0 0 1 1 2 1 2 3 2 0 2 2 1
Golgi 1 0 0 0 2 0 0 0 0 0 0 0 0 1 0 2 1 2 1 0 0 1 0 2
Mitochondria 3 1 1 6 24 80 130 122 182 127 232 122 0 1 0 4 4 2 2 4 5 2 5 5
Nucleus 3 1 0 1 0 3 5 13 7 10 8 5 0 0 2 3 3 3 3 2 0 0 1 0
Nuclear Periphery 2 0 0 1 1 1 0 1 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 2 3 1 1 0 0 0 0 0 3 0 1 1 3 9
Vac/Vac Membrane 15 5 3 12 29 20 26 15 20 20 10 22 33 30 23 19 14 13 2 1 2 2 1 3
Unique Cell Count 467 268 326 340 463 446 486 537 440 508 435 368 360 440 496 222 179 191 515 272 342 382 544 603
Labelled Cell Count 481 274 331 353 494 503 562 611 546 577 558 439 376 460 512 247 202 207 515 272 342 382 544 603


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.0 6.0 5.7 5.6 4.5 4.2 4.8 3.9 4.2 3.8 4.2 6.3 6.9 6.8 7.0 7.8 7.2 6.6 6.7 7.3
Std Deviation (1e-4) 0.9 1.2 0.8 1.5 1.4 1.1 1.2 1.2 1.2 1.2 1.0 1.2 1.1 1.9 1.5 1.5 1.5 1.7 1.8 1.7 2.2
Intensity Change (Log2) -0.07 -0.12 -0.44 -0.53 -0.34 -0.63 -0.51 -0.68 -0.52 0.05 0.19 0.16 0.22 0.37 0.26 0.13 0.15 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -3.4 -5.0 -7.4 -6.8 -9.2 -8.0 -10.7 -8.7 -2.4 -1.3 -0.4 -3.1 -3.1 -3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.3 3.7 2.9 3.3 1.9 2.9 2.6 1.5 3.6 4.8 4.0 3.0 4.5 4.1 3.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc43-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available