Standard name
Human Ortholog
Description Central component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0.08 0 0.05 0 0 0 0.14 0.09 0.06 0 0 0 0 0.05 0 0 0 0.1
Bud 0.05 0.06 0.09 0.09 0.16 0.16 0.13 0.16 0.14 0.12 0.14 0.08 0.07 0.07 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0.05 0.06 0.05 0 0.07 0.06 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0 0 0.05 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.45 0.62 0.54 0.38 0.44 0.69 0.5 0.48 0.48 0.61 0.57 0.51 0.27 0.25 0.06 0.05 0.14 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.05 0 0.05 0 0 0.06 0.05 0.08 0 0 0.07 0 0 0.14 0.17 0.13 0 0 0 0 0 0
Peroxisomes 0.74 0.66 0.71 0.77 0.7 0.59 0.63 0.63 0.49 0.5 0.56 0.68 0.86 0.85 0.72 0.73 0.7 0.85 0.75 0.79 0.77 0.78 0.76
SpindlePole 0.1 0.08 0.17 0.18 0.18 0.09 0.18 0.18 0.24 0.13 0.15 0.13 0.16 0.17 0.21 0.2 0.22 0.06 0 0.07 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 9 7 2 2 31 15 31 8 13 9 15 34 21 4 0 2 7 11 3 1 11 29
Bud 11 14 13 21 56 59 77 97 60 47 64 9 26 24 7 4 6 0 2 0 1 5 2
Bud Neck 0 1 1 1 1 0 1 1 1 0 2 0 1 0 0 0 1 0 2 0 1 3 2
Bud Site 10 5 5 9 18 24 26 14 29 24 19 1 6 4 3 4 4
Cell Periphery 0 2 0 0 0 1 3 0 1 3 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 8 12 6 11 19 13 9 12 1 4 4 6 3 4 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 1 2 2 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 1 3 0
Endosome 10 4 1 4 14 0 11 16 19 7 14 4 20 23 6 5 6 3 8 6 5 13 9
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 3 3 3 3 2 3 9 1 1 6 8
Mitochondria 106 140 78 84 156 259 287 297 201 238 261 54 104 91 11 7 18 4 11 2 3 11 11
Nucleus 1 0 0 0 0 1 2 3 0 3 0 0 1 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 2 3 1 0 6 6 15 1 10 5 8 0 2 1 1 0 0 0 0 0 0 0 0
Nucleolus 18 12 4 11 15 10 33 28 32 15 10 7 6 9 25 23 17 0 1 0 2 0 0
Peroxisomes 175 148 103 171 244 220 362 388 204 195 255 72 326 311 124 97 93 205 180 119 79 246 232
SpindlePole 24 17 24 40 62 33 103 114 100 50 70 14 61 61 37 27 29 13 10 10 1 9 6
Vac/Vac Membrane 1 2 1 0 0 0 0 0 4 2 3 0 2 0 0 0 2 0 0 0 0 0 1
Unique Cell Count 237 225 145 223 351 376 571 620 415 387 458 106 380 368 173 133 132 241 240 151 103 318 306
Labelled Cell Count 363 357 239 353 588 650 946 1010 682 611 729 177 597 553 228 174 184 241 240 151 103 318 306


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 10.1 6.6 6.7 5.9 5.7 5.1 5.4 5.0 5.3 5.4 8.4 8.8 8.8 12.6 14.4 14.2 9.1 10.1 10.1
Std Deviation (1e-4) 2.9 2.7 1.3 2.3 1.7 1.5 1.5 1.5 1.6 1.6 1.5 1.8 1.8 1.7 5.1 5.3 5.4 2.3 2.5 2.3
Intensity Change (Log2) 0.03 -0.15 -0.22 -0.37 -0.28 -0.4 -0.32 -0.29 0.35 0.41 0.43 0.94 1.13 1.11 0.46 0.61 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 1.3 0 0.1 0 -0.8 0 2.6 1.6 0.4 -1.2 0 0
Bud 0.1 2.0 2.0 1.5 2.1 1.7 1.0 1.6 -0.1 -0.8 -1.0 -1.8 -2.1 -1.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.3 0.8 1.3 0.6 0 1.5 1.2 0.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 2.2 0 0 0 2.4 2.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.0 -1.9 3.2 -0.8 -1.3 -1.1 1.6 0.7 -0.4 -5.7 -6.3 -9.4 -8.8 -7.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.0 0.8 0 1.5 1.0 2.1 0.6 0 0 0 0 3.6 4.1 3.2
Peroxisomes 1.2 -0.3 -2.6 -1.7 -1.9 -4.6 -4.3 -3.3 -0.5 3.9 3.5 0.1 0.4 -0.1
SpindlePole 0.3 0.3 -2.5 0.4 0.5 1.9 -1.1 -0.4 -0.7 -0.1 0 1.1 0.8 1.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5798 3.2973 3.8115 3.5409 3.2405 3.6429 5.4978 4.2791 5.0864 4.4323 4.8092 4.7411 3.5378 2.9035 2.5444 2.9766 2.5064 2.9687
Actin 0.005 0.0128 0.0209 0.0014 0.0007 0.0018 0.016 0.0196 0.0126 0.0015 0.0132 0.0021 0.0195 0.0166 0.0158 0.0013 0.0006 0.0044
Bud 0.0003 0.0004 0.0032 0.0003 0.0043 0 0.0001 0.0005 0.0027 0.0006 0 0 0.0002 0.0011 0.0032 0.006 0 0.0001
Bud Neck 0.0004 0.0003 0.0011 0.0002 0.0002 0.0002 0.0018 0.0004 0.0034 0.0001 0.0002 0.0001 0.0032 0.0008 0.0022 0.0001 0.0001 0.0013
Bud Periphery 0.0005 0.0003 0.0039 0.0007 0.0045 0 0.0001 0.0004 0.0029 0.0007 0 0 0.0002 0.0013 0.0042 0.0069 0 0.0004
Bud Site 0.0015 0.0057 0.0062 0.0005 0.0098 0.0002 0.0038 0.0226 0.0045 0.0026 0.0004 0.0001 0.0032 0.0229 0.0023 0.0032 0.0001 0.0003
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.0044 0.0002 0.0002 0 0.0002 0 0.001 0.0001 0.0002 0 0.0017 0 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001
Cytoplasmic Foci 0.1028 0.1037 0.1101 0.0901 0.1201 0.1038 0.0822 0.1398 0.0884 0.0753 0.1447 0.0786 0.0972 0.1284 0.093 0.0993 0.1595 0.1308
Eisosomes 0.0001 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0025 0 0.0001 0 0.0001 0 0.0005 0 0.0005 0 0.0012 0 0.0001 0 0.0001 0 0 0.0001
Endosome 0.0135 0.0096 0.0066 0.0055 0.0259 0.0046 0.0056 0.0027 0.0046 0.0015 0.0128 0.0025 0.0038 0.0058 0.0046 0.0018 0.0353 0.0032
Golgi 0.0102 0.016 0.0113 0.0195 0.0108 0.0121 0.0119 0.0088 0.009 0.0069 0.0224 0.0089 0.0136 0.0107 0.0087 0.0079 0.0062 0.0075
Lipid Particles 0.1263 0.0594 0.0819 0.0515 0.1611 0.152 0.1415 0.0467 0.0801 0.1278 0.1585 0.131 0.161 0.1133 0.0608 0.2068 0.1304 0.1553
Mitochondria 0.0199 0.0226 0.0184 0.02 0.0204 0.0172 0.02 0.0087 0.0206 0.014 0.0126 0.0106 0.0317 0.0175 0.0195 0.0247 0.0052 0.0127
None 0.0008 0.0001 0.0006 0.0001 0.0001 0 0.0004 0.0001 0.0002 0 0.0025 0 0.0003 0.0001 0.0003 0.0001 0 0.0002
Nuclear Periphery 0.0042 0 0.0001 0.0001 0.0001 0 0.0003 0 0.0003 0 0.0008 0 0.0001 0 0.0002 0 0 0.0008
Nucleolus 0.0073 0.0012 0.0041 0.0003 0.0026 0.0004 0.0048 0.0062 0.0006 0.0006 0.0002 0.0002 0.0034 0.0029 0.0006 0.0003 0.0003 0.0023
Nucleus 0.0059 0.0001 0.0004 0 0.0003 0 0.0002 0.0005 0.0002 0.0001 0.0001 0 0.0002 0.0006 0.0001 0.0001 0 0.0002
Peroxisomes 0.6775 0.755 0.716 0.8045 0.636 0.7058 0.7057 0.7219 0.7662 0.7645 0.6264 0.7644 0.6584 0.6741 0.7815 0.6402 0.65 0.677
Punctate Nuclear 0.0154 0.0109 0.0094 0.0051 0.0018 0.0015 0.0036 0.0202 0.0021 0.0035 0.0017 0.0015 0.0032 0.0017 0.0021 0.0008 0.0024 0.0027
Vacuole 0.0011 0.0011 0.0041 0.0001 0.0005 0.0001 0.0003 0.0004 0.0006 0.0001 0.0002 0.0001 0.0002 0.0014 0.0003 0.0003 0.0071 0.0003
Vacuole Periphery 0.0006 0.0004 0.0013 0.0001 0.0004 0.0001 0.0002 0.0002 0.0003 0.0001 0.0002 0.0001 0.0002 0.0005 0.0001 0.0001 0.0023 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.443 20.189 9.9874 14.1547 18.1267 13.973 20.8991 16.7298 20.8902 17.6674
Translational Efficiency 1.8627 1.1352 2.5052 1.9081 1.4991 1.9328 0.9313 1.5156 1.3531 1.5104

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1972 1378 305 860 1957 1823 171 1194 3929 3201 476 2054

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 838.46 904.21 1287.29 1235.13 839.71 971.99 1168.15 1136.14 839.08 942.81 1244.49 1177.59
Standard Deviation 134.00 180.24 203.16 194.92 142.00 185.41 162.69 223.21 138.05 186.25 198.05 217.38
Intensity Change Log 2 0.108916 0.618523 0.558849 0.211050 0.476262 0.436178 0.160925 0.549093 0.498771

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.026563 0.032146 0.048837 0.391356* 0.019636 0.026082 0.056437 0.319252* 0.023113 0.028692 0.051567 0.349442*
Bud Neck 0.001287 0.001880 0.008743 0.004229 0.001485 0.002817 0.005309 0.002465 0.001386 0.002414 0.007510 0.003203
Bud Site 0.002496 0.004007 0.013018 0.014176 0.003616 0.007389 0.018078 0.008725 0.003054 0.005933 0.014835 0.011007
Cell Periphery 0.000127 0.000098 0.000098 0.000160 0.000121 0.000109 0.000090 0.000159 0.000124 0.000104 0.000095 0.000159
Cytoplasm 0.002252 0.003189 0.003458 0.002895 0.003134 0.003908 0.002623 0.003415 0.002691 0.003599 0.003158 0.003198
Cytoplasmic Foci 0.048014 0.047941 0.105378 0.039213 0.043324 0.059829 0.069529 0.030687 0.045678 0.054711 0.092500 0.034257
Eisosomes 0.002638 0.009324 0.003344 0.003006 0.004649 0.002927 0.001571 0.005780 0.003639 0.005681 0.002707 0.004618
Endoplasmic Reticulum 0.000218 0.000121 0.000380 0.000594 0.000080 0.000189 0.000351 0.000725 0.000149 0.000159 0.000369 0.000671
Endosome 0.006590 0.004159 0.006725 0.014443 0.004491 0.007200 0.009452 0.009489 0.005545 0.005891 0.007705 0.011563
Golgi 0.058116 0.046575 0.036106 0.060624 0.042753 0.063175 0.021434 0.045678 0.050464 0.056029 0.030835 0.051936
Lipid Particles 0.048923 0.039285 0.040455 0.034879 0.029335 0.046436 0.018706 0.025154 0.039166 0.043357 0.032642 0.029226
Mitochondria 0.013845 0.014334 0.028488 0.032446 0.018006 0.039073 0.005139 0.032595 0.015918 0.028423 0.020100 0.032532
Mitotic Spindle 0.002904 0.003338 0.048143 0.012221 0.001806 0.007613 0.043117 0.003992 0.002357 0.005773 0.046337 0.007437
None 0.000222 0.000245 0.000180 0.000165 0.000187 0.000223 0.000080 0.000179 0.000205 0.000232 0.000144 0.000173
Nuclear Periphery 0.000036 0.000047 0.000365 0.000155 0.000030 0.000087 0.000107 0.000074 0.000033 0.000070 0.000272 0.000108
Nuclear Periphery Foci 0.000606 0.000326 0.000410 0.000165 0.000203 0.000402 0.000167 0.000162 0.000405 0.000370 0.000323 0.000163
Nucleolus 0.000160 0.000180 0.000449 0.000061 0.000098 0.000304 0.000119 0.000120 0.000129 0.000251 0.000330 0.000095
Nucleus 0.000099 0.000048 0.004686 0.000041 0.000033 0.000140 0.000114 0.000047 0.000066 0.000101 0.003044 0.000045
Peroxisomes 0.784409* 0.792394* 0.648140* 0.385313* 0.826798* 0.731294* 0.744531* 0.509138* 0.805523* 0.757597* 0.682768* 0.457293*
Vacuole 0.000382 0.000323 0.002251 0.002449 0.000186 0.000622 0.002984 0.001250 0.000284 0.000493 0.002514 0.001752
Vacuole Periphery 0.000113 0.000040 0.000346 0.001408 0.000030 0.000182 0.000061 0.000915 0.000072 0.000121 0.000244 0.001121

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.90 -3.01 -30.29* -29.53* -24.69* -2.86 -4.01 -29.96* -29.15* -19.63* -3.07 -4.96 -42.18* -41.16* -31.44*
Bud Neck -1.71 -5.94 -6.63 -4.65 3.43 -3.62 -4.77 -3.19 0.80 3.53 -4.06 -7.17 -7.10 -2.76 4.92
Bud Site -1.58 -5.41 -5.10 -4.28 -0.39 -3.42 -3.19 -4.23 -1.09 2.08 -3.87 -5.64 -6.73 -4.01 1.74
Cell Periphery 1.08 1.21 -1.27 -2.85 -3.46 0.54 1.09 -1.38 -2.31 -2.73 1.15 1.63 -1.83 -3.55 -4.17
Cytoplasm -2.32 -1.89 -1.83 0.57 0.85 -2.07 0.71 -0.81 1.27 -1.34 -3.33 -1.28 -2.04 1.30 0.16
Cytoplasmic Foci -0.01 -6.37 2.34 2.24 7.15 -4.73 -2.94 4.19 8.34 4.30 -3.73 -7.06 4.87 7.91 8.67
Eisosomes -6.91 -1.40 -0.98 6.42 0.62 2.86 4.79 -1.17 -3.07 -4.41 -3.85 2.30 -1.71 1.56 -3.20
Endoplasmic Reticulum 1.47 -1.90 -3.18 -4.85 -1.64 -4.99 -2.10 -1.39 -1.15 -0.64 -0.27 -3.22 -1.90 -1.88 -0.94
Endosome 3.04 -0.10 -4.88 -6.51 -4.32 -2.91 -2.39 -3.94 -1.78 -0.00 -0.55 -2.05 -6.04 -5.67 -2.95
Golgi 3.47 5.05 -0.56 -3.06 -4.57 -6.72 6.08 -0.85 4.60 -5.77 -2.44 6.50 -0.52 1.41 -5.94
Lipid Particles 2.45 1.40 3.44 1.08 0.84 -5.01 2.41 1.32 5.82 -1.37 -1.59 1.58 3.86 5.16 0.77
Mitochondria -0.26 -2.79 -6.07 -5.66 -0.70 -7.10 7.24 -4.72 1.71 -9.27 -6.70 -1.19 -7.59 -1.59 -3.24
Mitotic Spindle -0.44 -6.12 -4.46 -4.03 4.70 -5.54 -4.63 -3.16 2.96 4.37 -4.70 -7.69 -5.26 -1.54 6.72
None -0.61 1.25 1.73 4.93 0.83 -1.66 6.77 0.53 2.00 -6.03 -1.32 3.09 1.72 4.04 -2.21
Nuclear Periphery -2.39 -6.62 -4.41 -4.02 3.73 -11.61 -4.87 -2.26 0.60 1.36 -10.37 -7.32 -4.66 -2.37 4.52
Nuclear Periphery Foci 1.22 0.92 2.05 1.61 2.59 -2.56 0.34 0.44 2.26 0.23 0.32 0.70 2.03 2.76 2.15
Nucleolus -0.23 -2.11 1.80 3.01 3.03 -2.69 -0.55 -0.64 1.72 -0.26 -2.20 -2.32 0.55 2.49 2.53
Nucleus 1.33 -1.42 1.53 0.64 1.43 -1.99 -1.42 -1.49 1.63 1.18 -0.95 -1.43 0.97 1.70 1.44
Peroxisomes -0.95 7.67 31.54* 31.09* 12.44 12.06 4.29 28.65* 18.54 10.43 8.27 9.16 41.10* 33.48 14.37
Vacuole 0.40 -2.58 -2.59 -2.69 -0.13 -4.97 -1.19 -2.69 -1.58 0.87 -2.44 -2.07 -3.62 -3.12 0.91
Vacuole Periphery 1.04 -2.10 -2.16 -2.30 -1.76 -2.48 -2.64 -2.23 -1.82 -2.14 -0.98 -2.63 -3.07 -2.93 -2.54