Standard name
Human Ortholog
Description Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0 0 0.1 0.07 0.09 0.07 0.06 0.07 0.06 0.08 0.15 0.18 0.09 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0 0.28 0.11 0.09 0.09 0.58 0.19 0.3 0.41 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.9 0.89 0.96 0.96 0.85 0.8 0.82 0.81 0.67 0.8 0.74 0.65 0.89 0.78 0.74 0.81 0.76 0.73 0.89 0.87 0.92 0.79 0.7 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0 0 0.05 0.06 0.05 0 0.06 0.05 0.09 0 0.07 0.06 0.11 0.16 0.16 0.05 0.05 0 0.09 0.15 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 5 0 2 0 2 4 1 5 0 0 0 0 0 0 1 2 0 8 6 6
Bud Neck 0 0 0 0 1 0 0 2 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 2 2 1 1 6 3 1 21 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 24 2 2 4 22 13 23 19 12 19 24 29 75 92 28 28 33 3 8 1 9 9 5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 15 20 0 1 0 0 0 1
Endosome 0 0 0 1 0 1 1 1 0 0 0 1 3 5 15 1 10 6 4 2 0 1 2 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1 1 1 1 1
Mitochondria 3 35 0 4 43 23 16 22 151 37 81 152 3 1 4 14 11 5 0 6 0 6 6 8
Nucleus 260 367 121 145 130 170 153 198 173 159 203 245 333 394 367 247 262 221 271 378 138 279 244 275
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 2
Nucleolus 1 1 0 1 2 2 5 1 4 1 3 16 1 0 5 0 2 0 0 0 0 3 8 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 1 1 1 1 0 0 2 1 1 2 4 3 1 0 4 9 4
Vac/Vac Membrane 26 18 3 2 6 11 12 13 10 11 15 33 10 36 29 33 56 47 13 23 5 30 50 76
Unique Cell Count 289 414 126 151 153 212 187 244 259 198 274 375 375 502 499 306 346 302 303 435 151 351 348 406
Labelled Cell Count 302 445 126 156 193 232 205 262 366 228 324 498 381 514 518 338 386 336 303 435 151 351 348 406


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.5 6.9 6.3 4.7 5.7 5.8 5.7 4.6 5.1 5.1 4.8 6.9 6.6 6.3 6.2 7.8 7.6 7.1 7.4 7.4
Std Deviation (1e-4) 1.3 0.9 1.0 0.9 1.2 1.4 1.3 1.2 1.0 1.0 1.0 0.9 1.3 1.3 1.5 1.6 1.8 2.0 1.7 1.8 1.4
Intensity Change (Log2) – – – -0.14 -0.55 -0.27 -0.26 -0.27 -0.59 -0.42 -0.43 -0.51 -0.01 -0.07 -0.14 -0.14 0.19 0.15 0.05 0.1 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 3.0 2.2 2.8 2.3 1.9 2.2 2.1 2.5 4.1 4.7 2.8 2.6 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 3.4 3.5 0 5.2 6.8 0 0 0 0 0 0 0
Nucleus 0 -3.1 -4.1 -3.7 -3.9 -6.3 -4.0 -5.2 -6.7 -2.4 -4.6 -5.5 -4.1 -5.0 -5.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.3 1.6 1.3 0 1.4 1.4 2.4 0 2.0 1.6 2.9 4.0 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.7334 6.8496 6.7592 6.857 6.3277 6.9998 8.4433 9.5338 8.2776 8.899 8.0782 8.6599 4.5933 5.1024 4.7825 4.8911 4.7421 5.0634
Actin 0.0313 0 0.0059 0.0001 0.0177 0.0006 0.0069 0 0.0045 0 0.0011 0.0001 0.0166 0 0.0055 0.0006 0.0002 0
Bud 0.0005 0 0.0005 0 0.001 0 0.0002 0 0.0001 0 0.0001 0 0.0003 0.0011 0.0002 0.0001 0 0
Bud Neck 0.0027 0.0001 0.001 0.0001 0.0022 0.0007 0.0005 0.0001 0.0009 0.0001 0.0148 0.0004 0.001 0.0001 0.0002 0.0003 0.0003 0.0008
Bud Periphery 0.0008 0 0.001 0 0.003 0.0001 0.0007 0 0.0006 0 0.0002 0 0.0008 0.0002 0.0001 0.0004 0.0001 0
Bud Site 0.0027 0.0001 0.005 0 0.0018 0.0001 0.0007 0.0004 0.0002 0 0.0003 0 0.0006 0.0001 0.0045 0.0001 0.0002 0
Cell Periphery 0.0002 0 0.0004 0 0.0012 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0 0.0001 0 0
Cytoplasm 0.0071 0.0003 0.0039 0.0001 0.003 0.0003 0.0011 0.0005 0.0004 0.0001 0.0003 0.0001 0.0021 0.0004 0.0018 0.0002 0.0005 0.0001
Cytoplasmic Foci 0.0066 0 0.002 0.0019 0.0074 0.0002 0.0014 0 0.0006 0 0.0027 0.0009 0.0034 0 0.0024 0.0003 0.0073 0
Eisosomes 0.0006 0 0 0 0.0004 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0036 0 0.0024 0.0003 0.0054 0.0006 0.0034 0 0.0008 0 0.0002 0 0.0071 0 0.0007 0.0011 0.0002 0
Endosome 0.0093 0 0.0053 0.0154 0.0109 0.0008 0.0103 0.0002 0.0018 0 0.0125 0.0007 0.011 0 0.0058 0.0024 0.0085 0
Golgi 0.0032 0 0.0025 0.0076 0.005 0.0002 0.0039 0 0.001 0 0.01 0.0002 0.0045 0 0.0056 0.0007 0.0099 0
Lipid Particles 0.0041 0 0.0025 0.0269 0.0174 0.0007 0.0085 0 0.0015 0 0.0006 0.0009 0.0053 0 0.0009 0.0087 0.0067 0
Mitochondria 0.007 0.0001 0.0045 0.0009 0.0374 0.0004 0.0048 0 0.0025 0.0001 0.002 0.0001 0.0035 0.0002 0.0021 0.0029 0.0034 0.0001
None 0.0038 0 0.0052 0 0.0048 0.0001 0.0008 0 0.0001 0 0.0001 0 0.0014 0.0001 0.0011 0.0002 0.0002 0
Nuclear Periphery 0.0254 0.002 0.009 0.0023 0.0104 0.002 0.0181 0.002 0.0034 0.0014 0.0008 0.0003 0.0263 0.0014 0.0068 0.0257 0.0028 0.0007
Nucleolus 0.0068 0.0068 0.0058 0.0012 0.009 0.014 0.0061 0.0052 0.0057 0.0108 0.0088 0.0091 0.0086 0.0056 0.0023 0.0065 0.0168 0.0128
Nucleus 0.8606 0.9896 0.9333 0.94 0.8511 0.9781 0.9233 0.9877 0.9729 0.987 0.9429 0.9848 0.8955 0.9895 0.9561 0.9444 0.9245 0.9827
Peroxisomes 0.007 0 0.0034 0.0002 0.0026 0.0001 0.0007 0 0.0003 0 0.0004 0.0011 0.0015 0 0.0009 0.0002 0.0168 0
Punctate Nuclear 0.0136 0.0008 0.0033 0.0004 0.0033 0.0008 0.0028 0.0005 0.0014 0.0001 0.0013 0.0011 0.0068 0.0011 0.0025 0.0014 0.0007 0.0025
Vacuole 0.0016 0.0001 0.0021 0.001 0.0031 0.0002 0.004 0.0033 0.0006 0.0003 0.0006 0 0.0018 0.0001 0.0002 0.0014 0.0006 0.0001
Vacuole Periphery 0.0016 0 0.0009 0.0014 0.0017 0.0001 0.0016 0.0001 0.0005 0.0002 0.0003 0 0.0017 0 0.0002 0.0022 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.7678 51.4072 30.1992 27.9849 24.8532 21.553 30.3204 31.0618 27.8499 19.7452
Translational Efficiency 0.9823 0.6996 1.0845 1.0209 0.9899 0.7004 1.2461 1.095 0.9348 1.2426

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1866 1060 521 1548 2162 1735 137 1129 4028 2795 658 2677

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 779.32 829.54 1032.15 1096.78 713.63 900.57 1128.24 1082.92 744.06 873.63 1052.16 1090.93
Standard Deviation 106.13 155.55 147.91 221.60 106.29 117.43 136.79 164.15 111.15 137.57 150.80 199.52
Intensity Change Log 2 — 0.090096 0.405365 0.492986 — 0.335662 0.660826 0.601678 — 0.212698 0.533126 0.545964

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000095 0.000755 0.000578 0.000929 0.000075 0.000748 0.001195 0.001618 0.000084 0.000750 0.000706 0.001219
Bud Neck 0.001750 0.006009 0.010090 0.033381 0.003013 0.006077 0.005889 0.025302 0.002428 0.006051 0.009215 0.029974
Bud Site 0.000409 0.003191 0.000861 0.006716 0.001021 0.002697 0.000988 0.006042 0.000737 0.002884 0.000888 0.006431
Cell Periphery 0.000150 0.000297 0.000055 0.000119 0.000156 0.000165 0.000054 0.000178 0.000153 0.000215 0.000055 0.000144
Cytoplasm 0.036983 0.028523 0.000715 0.007712 0.045270 0.012534 0.000980 0.009218 0.041431 0.018598 0.000770 0.008347
Cytoplasmic Foci 0.002848 0.003201 0.000013 0.001772 0.002224 0.002856 0.000051 0.001798 0.002513 0.002987 0.000021 0.001783
Eisosomes 0.000011 0.000024 0.000024 0.000024 0.000009 0.000017 0.000052 0.000031 0.000010 0.000020 0.000030 0.000027
Endoplasmic Reticulum 0.002204 0.006912 0.004124 0.004643 0.001645 0.004211 0.007799 0.006401 0.001904 0.005235 0.004889 0.005384
Endosome 0.000526 0.002381 0.000114 0.004754 0.000364 0.001369 0.000159 0.002859 0.000439 0.001753 0.000124 0.003955
Golgi 0.000302 0.001869 0.000031 0.003061 0.000058 0.001008 0.000052 0.004105 0.000171 0.001335 0.000035 0.003501
Lipid Particles 0.001518 0.000676 0.000043 0.000171 0.000338 0.001156 0.000168 0.000359 0.000885 0.000974 0.000069 0.000250
Mitochondria 0.002460 0.002670 0.000964 0.005832 0.000660 0.003036 0.001039 0.005982 0.001494 0.002897 0.000980 0.005895
Mitotic Spindle 0.000870 0.002889 0.000590 0.047888 0.000321 0.003015 0.000084 0.017584 0.000575 0.002967 0.000484 0.035108
None 0.007947 0.017882 0.000960 0.003729 0.012402 0.005203 0.000684 0.006515 0.010338 0.010011 0.000903 0.004904
Nuclear Periphery 0.000689 0.000653 0.000262 0.004142 0.000597 0.001160 0.000305 0.002440 0.000640 0.000968 0.000271 0.003424
Nuclear Periphery Foci 0.000759 0.001146 0.000024 0.003488 0.000194 0.000551 0.000121 0.001879 0.000455 0.000776 0.000044 0.002810
Nucleolus 0.001882 0.006359 0.001659 0.002636 0.002426 0.004146 0.002138 0.002808 0.002174 0.004986 0.001759 0.002709
Nucleus 0.936930* 0.908807* 0.977591* 0.849435* 0.927408* 0.945948* 0.974949* 0.892755* 0.931819* 0.931863* 0.977041* 0.867705*
Peroxisomes 0.000237 0.001510 0.000103 0.000245 0.000222 0.001419 0.000237 0.000564 0.000229 0.001454 0.000131 0.000379
Vacuole 0.001285 0.003264 0.001139 0.011474 0.001301 0.002250 0.002983 0.009069 0.001293 0.002634 0.001523 0.010460
Vacuole Periphery 0.000146 0.000983 0.000062 0.007852 0.000296 0.000433 0.000075 0.002491 0.000227 0.000641 0.000065 0.005591

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.55 -12.48 -7.88 -1.34 -3.38 -1.76 -10.74 -6.74 -1.87 0.03 -2.71 -15.89 -10.14 -1.92 -3.58
Bud Neck -6.42 -9.93 -15.72 -12.94 -10.14 -3.59 -5.04 -14.03 -11.47 -10.53 -6.34 -10.20 -20.63 -16.96 -13.22
Bud Site -3.31 -3.69 -8.37 -2.18 -7.36 -1.92 -1.71 -5.63 -2.36 -4.50 -3.48 -1.89 -9.64 -3.32 -9.09
Cell Periphery -2.96 3.50 1.63 4.45 -4.76 -0.39 4.62 0.34 0.68 -3.81 -2.54 5.97 1.77 4.29 -6.19
Cytoplasm 2.09 15.24 11.25 6.42 -8.98 11.60 11.17 13.14 1.86 -2.10 10.07 21.87 17.41 6.35 -8.88
Cytoplasmic Foci -0.30 5.64 3.95 3.77 -3.69 -0.85 5.34 2.75 3.07 -2.49 -0.76 7.87 4.85 4.77 -4.42
Eisosomes -6.35 -9.95 -12.59 -1.71 -1.96 -5.36 -11.34 -11.84 -7.24 4.56 -8.13 -14.55 -17.39 -7.07 0.06
Endoplasmic Reticulum -11.29 -3.68 -10.33 3.34 -1.88 -9.11 -9.15 -13.23 -6.59 1.42 -14.02 -7.07 -17.05 -3.13 -2.13
Endosome -3.27 2.24 -3.28 0.54 -4.57 -3.40 1.76 -3.97 -0.70 -4.30 -4.57 3.26 -4.92 -0.32 -6.21
Golgi -2.19 1.90 -2.56 0.76 -3.66 -3.12 -1.39 -2.12 -0.97 -2.09 -3.51 1.93 -3.21 -0.40 -3.58
Lipid Particles 1.58 2.87 2.57 4.23 -7.18 -3.12 1.01 -0.97 2.79 -2.45 -0.41 3.30 2.39 4.10 -6.54
Mitochondria -0.18 1.49 -2.07 -2.29 -7.13 -3.29 -3.26 -4.36 -1.36 -3.73 -2.13 0.81 -4.91 -2.42 -7.22
Mitotic Spindle -1.80 -0.79 -8.58 -7.68 -7.23 -2.87 -0.35 -6.30 -5.11 -6.26 -3.28 -0.93 -10.61 -9.24 -8.50
None -3.87 8.28 4.00 5.59 -8.62 4.95 9.78 5.33 0.35 -4.69 0.28 12.66 6.92 4.84 -8.58
Nuclear Periphery 0.39 5.59 -5.38 -5.44 -6.15 -2.80 1.11 -8.66 -5.51 -8.91 -2.55 5.89 -8.27 -6.76 -9.21
Nuclear Periphery Foci -0.90 1.95 -1.27 -0.59 -3.00 -2.24 -0.61 -2.65 -1.58 -2.25 -1.49 2.10 -2.55 -1.65 -3.94
Nucleolus -5.62 0.78 -4.00 3.03 -4.42 -3.13 0.46 -2.51 0.06 -2.55 -6.19 1.53 -4.40 1.98 -5.19
Nucleus 3.99 -8.40 8.78 3.71 15.28 -3.44 -6.26 5.54 7.90 9.82 0.14 -11.81 10.20 9.18 18.79
Peroxisomes -1.88 2.11 0.64 1.96 -2.23 -2.86 0.02 -0.55 2.70 -0.93 -3.38 1.34 -0.13 3.41 -2.47
Vacuole -3.80 0.31 -8.85 -6.61 -8.78 -2.43 -3.64 -7.38 -6.79 -5.10 -4.31 -1.95 -11.38 -9.77 -10.45
Vacuole Periphery -2.19 1.31 -3.84 -2.54 -3.95 -0.71 1.15 -2.09 -1.89 -2.74 -2.28 1.56 -4.29 -3.38 -4.69
Standard name
Human Ortholog
Description Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Lot5

Lot5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lot5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available