Standard name
Description Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0 0 0.1 0.07 0.09 0.07 0.06 0.07 0.06 0.08 0.15 0.18 0.09 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0 0.28 0.11 0.09 0.09 0.58 0.19 0.3 0.41 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.9 0.89 0.96 0.96 0.85 0.8 0.82 0.81 0.67 0.8 0.74 0.65 0.89 0.78 0.74 0.81 0.76 0.73 0.89 0.87 0.92 0.79 0.7 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0 0 0.05 0.06 0.05 0 0.06 0.05 0.09 0 0.07 0.06 0.11 0.16 0.16 0.05 0.05 0 0.09 0.15 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 5 0 2 0 2 4 1 5 0 0 0 0 0 0 1 2 0 8 6 6
Bud Neck 0 0 0 0 1 0 0 2 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 2 2 1 1 6 3 1 21 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 24 2 2 4 22 13 23 19 12 19 24 29 75 92 28 28 33 3 8 1 9 9 5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 15 20 0 1 0 0 0 1
Endosome 0 0 0 1 0 1 1 1 0 0 0 1 3 5 15 1 10 6 4 2 0 1 2 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1 1 1 1 1
Mitochondria 3 35 0 4 43 23 16 22 151 37 81 152 3 1 4 14 11 5 0 6 0 6 6 8
Nucleus 260 367 121 145 130 170 153 198 173 159 203 245 333 394 367 247 262 221 271 378 138 279 244 275
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 2
Nucleolus 1 1 0 1 2 2 5 1 4 1 3 16 1 0 5 0 2 0 0 0 0 3 8 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 1 1 1 1 0 0 2 1 1 2 4 3 1 0 4 9 4
Vac/Vac Membrane 26 18 3 2 6 11 12 13 10 11 15 33 10 36 29 33 56 47 13 23 5 30 50 76
Unique Cell Count 289 414 126 151 153 212 187 244 259 198 274 375 375 502 499 306 346 302 303 435 151 351 348 406
Labelled Cell Count 302 445 126 156 193 232 205 262 366 228 324 498 381 514 518 338 386 336 303 435 151 351 348 406


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.5 6.9 6.3 4.7 5.7 5.8 5.7 4.6 5.1 5.1 4.8 6.9 6.6 6.3 6.2 7.8 7.6 7.1 7.4 7.4
Std Deviation (1e-4) 1.3 0.9 1.0 0.9 1.2 1.4 1.3 1.2 1.0 1.0 1.0 0.9 1.3 1.3 1.5 1.6 1.8 2.0 1.7 1.8 1.4
Intensity Change (Log2) -0.14 -0.55 -0.27 -0.26 -0.27 -0.59 -0.42 -0.43 -0.51 -0.01 -0.07 -0.14 -0.14 0.19 0.15 0.05 0.1 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 3.0 2.2 2.8 2.3 1.9 2.2 2.1 2.5 4.1 4.7 2.8 2.6 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 3.4 3.5 0 5.2 6.8 0 0 0 0 0 0 0
Nucleus 0 -3.1 -4.1 -3.7 -3.9 -6.3 -4.0 -5.2 -6.7 -2.4 -4.6 -5.5 -4.1 -5.0 -5.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.3 1.6 1.3 0 1.4 1.4 2.4 0 2.0 1.6 2.9 4.0 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.7334 6.8496 6.7592 6.857 6.3277 6.9998 8.4433 9.5338 8.2776 8.899 8.0782 8.6599 4.5933 5.1024 4.7825 4.8911 4.7421 5.0634
Actin 0.0313 0 0.0059 0.0001 0.0177 0.0006 0.0069 0 0.0045 0 0.0011 0.0001 0.0166 0 0.0055 0.0006 0.0002 0
Bud 0.0005 0 0.0005 0 0.001 0 0.0002 0 0.0001 0 0.0001 0 0.0003 0.0011 0.0002 0.0001 0 0
Bud Neck 0.0027 0.0001 0.001 0.0001 0.0022 0.0007 0.0005 0.0001 0.0009 0.0001 0.0148 0.0004 0.001 0.0001 0.0002 0.0003 0.0003 0.0008
Bud Periphery 0.0008 0 0.001 0 0.003 0.0001 0.0007 0 0.0006 0 0.0002 0 0.0008 0.0002 0.0001 0.0004 0.0001 0
Bud Site 0.0027 0.0001 0.005 0 0.0018 0.0001 0.0007 0.0004 0.0002 0 0.0003 0 0.0006 0.0001 0.0045 0.0001 0.0002 0
Cell Periphery 0.0002 0 0.0004 0 0.0012 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0 0.0001 0 0
Cytoplasm 0.0071 0.0003 0.0039 0.0001 0.003 0.0003 0.0011 0.0005 0.0004 0.0001 0.0003 0.0001 0.0021 0.0004 0.0018 0.0002 0.0005 0.0001
Cytoplasmic Foci 0.0066 0 0.002 0.0019 0.0074 0.0002 0.0014 0 0.0006 0 0.0027 0.0009 0.0034 0 0.0024 0.0003 0.0073 0
Eisosomes 0.0006 0 0 0 0.0004 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0036 0 0.0024 0.0003 0.0054 0.0006 0.0034 0 0.0008 0 0.0002 0 0.0071 0 0.0007 0.0011 0.0002 0
Endosome 0.0093 0 0.0053 0.0154 0.0109 0.0008 0.0103 0.0002 0.0018 0 0.0125 0.0007 0.011 0 0.0058 0.0024 0.0085 0
Golgi 0.0032 0 0.0025 0.0076 0.005 0.0002 0.0039 0 0.001 0 0.01 0.0002 0.0045 0 0.0056 0.0007 0.0099 0
Lipid Particles 0.0041 0 0.0025 0.0269 0.0174 0.0007 0.0085 0 0.0015 0 0.0006 0.0009 0.0053 0 0.0009 0.0087 0.0067 0
Mitochondria 0.007 0.0001 0.0045 0.0009 0.0374 0.0004 0.0048 0 0.0025 0.0001 0.002 0.0001 0.0035 0.0002 0.0021 0.0029 0.0034 0.0001
None 0.0038 0 0.0052 0 0.0048 0.0001 0.0008 0 0.0001 0 0.0001 0 0.0014 0.0001 0.0011 0.0002 0.0002 0
Nuclear Periphery 0.0254 0.002 0.009 0.0023 0.0104 0.002 0.0181 0.002 0.0034 0.0014 0.0008 0.0003 0.0263 0.0014 0.0068 0.0257 0.0028 0.0007
Nucleolus 0.0068 0.0068 0.0058 0.0012 0.009 0.014 0.0061 0.0052 0.0057 0.0108 0.0088 0.0091 0.0086 0.0056 0.0023 0.0065 0.0168 0.0128
Nucleus 0.8606 0.9896 0.9333 0.94 0.8511 0.9781 0.9233 0.9877 0.9729 0.987 0.9429 0.9848 0.8955 0.9895 0.9561 0.9444 0.9245 0.9827
Peroxisomes 0.007 0 0.0034 0.0002 0.0026 0.0001 0.0007 0 0.0003 0 0.0004 0.0011 0.0015 0 0.0009 0.0002 0.0168 0
Punctate Nuclear 0.0136 0.0008 0.0033 0.0004 0.0033 0.0008 0.0028 0.0005 0.0014 0.0001 0.0013 0.0011 0.0068 0.0011 0.0025 0.0014 0.0007 0.0025
Vacuole 0.0016 0.0001 0.0021 0.001 0.0031 0.0002 0.004 0.0033 0.0006 0.0003 0.0006 0 0.0018 0.0001 0.0002 0.0014 0.0006 0.0001
Vacuole Periphery 0.0016 0 0.0009 0.0014 0.0017 0.0001 0.0016 0.0001 0.0005 0.0002 0.0003 0 0.0017 0 0.0002 0.0022 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.7678 51.4072 30.1992 27.9849 24.8532 21.553 30.3204 31.0618 27.8499 19.7452
Translational Efficiency 0.9823 0.6996 1.0845 1.0209 0.9899 0.7004 1.2461 1.095 0.9348 1.2426

Hit Data

Dataset Hit
Protein Abundance
Protein Localization
Gene Expression
Translational Efficiency