Standard name
Description Mitochondrial ribosomal protein of the small subunit; has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 – 0.06 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.31 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0 0 0.07 – 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.32 0.38 0.36 – 0.12 – 0.06 0.05 0 0 0.05 0.07
Mitochondria 0.92 0.94 0.88 0.88 0.77 0.86 0.76 0.81 0.91 0.85 0.94 0.86 0.76 0.64 0.64 – 0.06 – 0.81 0.81 0.81 0.72 0.7 0.73
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0.1 0.12 0.07 0.07 0.08 0.09 0.12 0.1 0.09 0.1 0.08 0.11 0.05 0.05 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0.06 0.07 0.06 0.13 0.13 0.15 0.11 0.09 0.09 0.08 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.1 0.08 0.05 0.06 0.06 0 0.06 0 0.08 0 0 0 – 0.06 – 0 0 0.09 0.1 0.1 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.11 0 0 0.09 0 0.05 0 0 0 0 0 0.12 0.21 0.24 – 0.31 – 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 1 0 0 0 0 6 2 – 1 – 1 4 4 3 10 10
Bud 1 0 6 5 2 9 7 12 13 8 18 22 0 2 2 – 0 – 2 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 3 1
Bud Site 0 0 0 3 1 1 3 5 2 2 6 2 0 1 0 – 1 – – – – – – –
Cell Periphery 0 5 11 8 2 1 7 4 1 2 0 4 2 9 4 – 1 – 0 0 0 0 0 0
Cytoplasm 0 1 8 10 14 7 5 10 2 8 3 7 1 8 4 – 5 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 0 0 1 2 0 1 2 1 0 4 0 3 2 – 1 – 0 0 0 0 1 0
Endosome 6 0 19 6 29 15 35 17 6 6 2 11 0 8 12 – 0 – 3 4 11 6 12 10
Golgi 6 4 5 1 3 0 2 1 0 1 0 1 13 76 59 – 2 – 11 9 13 7 18 18
Mitochondria 163 170 382 323 302 458 455 617 416 412 448 538 31 127 106 – 1 – 146 149 355 143 243 202
Nucleus 1 0 0 0 0 3 7 3 0 3 0 3 0 3 1 – 0 – 0 0 0 0 0 0
Nuclear Periphery 17 22 30 26 30 50 72 79 42 48 39 66 2 10 5 – 0 – 1 1 2 1 3 2
Nucleolus 7 5 25 25 24 67 77 114 52 45 45 49 1 0 0 – 0 – 1 0 2 2 1 2
Peroxisomes 6 3 48 36 30 29 36 48 19 30 21 47 0 3 7 – 1 – 6 7 40 20 33 20
SpindlePole 3 0 5 4 5 10 9 28 11 5 8 13 0 4 2 – 0 – 1 0 3 0 1 0
Vac/Vac Membrane 22 20 14 9 34 12 30 17 14 16 14 13 5 42 40 – 5 – 1 2 1 10 10 5
Unique Cell Count 178 180 432 368 391 531 599 766 455 484 479 622 41 199 166 16 180 185 439 199 345 279
Labelled Cell Count 232 231 553 457 477 664 745 956 581 587 604 780 55 302 246 18 180 185 439 199 345 279


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 10.1 9.3 9.0 9.7 7.9 8.5 8.2 7.5 8.2 7.5 8.1 9.5 9.1 9.1 – 9.8 – 10.0 10.6 11.5
Std Deviation (1e-4) 2.2 2.2 2.0 2.2 2.3 2.1 2.6 2.4 2.0 6.4 2.2 2.6 1.5 1.5 1.5 – 3.3 – 2.0 2.7 2.7
Intensity Change (Log2) – – – -0.05 0.06 -0.22 -0.13 -0.18 -0.31 -0.18 -0.31 -0.21 0.04 -0.02 -0.03 – 0.08 – 0.1 0.19 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud 0 0 0.4 -0.3 0.2 1.5 0.3 2.2 2.1 0 0 0 – 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Cell Periphery -0.3 -2.3 -3.3 -1.7 -3.0 -3.0 -2.7 -3.5 -2.6 0 1.3 0 – 0 –
Cytoplasm 0.8 1.5 -0.7 -1.4 -0.7 0 -0.2 -1.7 -1.0 0 1.6 0 – 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Endosome -2.2 1.8 -1.3 1.0 -2.1 -2.8 -2.9 -4.0 -2.5 0 -0.2 1.4 – 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 12.9 12.2 – 0 –
Mitochondria -0.3 -4.3 -1.0 -5.1 -3.5 1.5 -1.5 2.7 -0.9 -2.4 -7.3 -6.9 – -9.2 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Nuclear Periphery 0.1 0.4 1.4 2.7 1.9 1.2 1.6 0.7 2.0 0 -0.9 -1.8 – 0 –
Nucleolus 0.6 0.2 3.6 3.8 4.7 3.0 2.0 2.0 1.3 0 -3.5 -3.2 – 0 –
Peroxisomes -0.6 -1.7 -3.2 -3.0 -3.0 -3.9 -2.7 -3.8 -2.0 0 -4.1 -2.6 – 0 –
SpindlePole 0 0 0.9 0.5 2.5 1.4 0 0.7 1.1 0 0 0 – 0 –
Vacuole -0.7 3.3 -0.9 1.4 -1.1 -0.1 0.1 -0.3 -1.2 0 7.3 8.0 – 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.7351 8.3953 8.0509 7.5851 7.6455 8.5534 9.148 9.8602 9.2284 8.5253 8.3419 9.5046 11.1699 10.3519 9.9132 9.73 8.7638 10.5563
Actin 0.0379 0.0029 0.0026 0.0026 0.0039 0.0027 0.0091 0.0162 0.0143 0.0006 0.0025 0.0012 0.0089 0.0012 0.028 0.0104 0.0012 0.0004
Bud 0.0006 0.0011 0.0002 0.0006 0.0002 0.0001 0.0008 0.0001 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Bud Neck 0.0015 0.0001 0.0001 0.0002 0 0.0023 0.0007 0.0001 0.0001 0 0.0001 0 0.0001 0.0002 0.0001 0.0002 0.0001 0
Bud Periphery 0.0012 0.0016 0.0004 0.0011 0.0006 0.0006 0.0027 0.0002 0.0007 0 0.0002 0.0003 0.0004 0.0002 0.0002 0.0002 0.0002 0.0001
Bud Site 0.0014 0.0014 0.0004 0.0009 0.0001 0.0001 0.0031 0.0005 0.0003 0 0.0004 0.0001 0.0004 0.0005 0.0004 0.0004 0.0003 0
Cell Periphery 0.0004 0.0004 0.0002 0.0004 0.0001 0.001 0.0002 0.0002 0.0002 0 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.003 0.0008 0.0001 0.0019 0 0.0015 0.0007 0 0.0022 0 0.0001 0.0003 0.0021 0 0.0002 0.002 0.0004 0
Cytoplasmic Foci 0.0111 0.0021 0.0028 0.0081 0.0003 0.0003 0.0183 0.0016 0.0017 0.0008 0.0041 0.0006 0.0031 0.0023 0.0036 0.0143 0.0087 0.0005
Eisosomes 0.0004 0.0002 0.0001 0.0002 0.0002 0.0002 0.0003 0.0002 0.0004 0 0.0001 0.0001 0.0003 0.0001 0.0004 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0026 0.0003 0.0002 0.0045 0.0001 0.0042 0.0005 0 0.0009 0 0.0003 0.0002 0.002 0 0.0001 0.003 0.0007 0.0001
Endosome 0.0246 0.0122 0.0079 0.0613 0.0017 0.0044 0.0264 0.0042 0.0074 0.0051 0.0138 0.0026 0.0073 0.0071 0.006 0.0122 0.0755 0.0051
Golgi 0.0214 0.0168 0.0096 0.0317 0.0048 0.0042 0.0214 0.0179 0.0081 0.0363 0.0102 0.0101 0.0089 0.0173 0.0083 0.0206 0.0208 0.0143
Lipid Particles 0.0243 0.0062 0.0097 0.0357 0.0002 0.0014 0.0097 0.0016 0.006 0.0006 0.0639 0.01 0.0364 0.0125 0.0167 0.0138 0.0211 0.0025
Mitochondria 0.8269 0.9348 0.9432 0.8061 0.9856 0.9616 0.871 0.9524 0.9385 0.9542 0.879 0.9528 0.8948 0.9434 0.924 0.8971 0.8559 0.9606
None 0.0035 0.0002 0.0001 0.0061 0 0.0002 0.0003 0 0.0053 0 0.0001 0.0004 0.0061 0 0.0001 0.0021 0.0002 0
Nuclear Periphery 0.0088 0.0003 0.0001 0.0091 0 0.0026 0.0019 0 0.0009 0 0.0006 0.0024 0.0061 0 0 0.0012 0.0015 0.0001
Nucleolus 0.0028 0.0008 0.0001 0.0009 0 0.0002 0.0005 0 0.0003 0 0.0003 0.0003 0.0003 0.0006 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0058 0.0003 0.0001 0.0019 0 0.0004 0.0007 0 0.0004 0 0.0001 0.0013 0.0013 0.0001 0.0001 0.0002 0.0002 0
Peroxisomes 0.006 0.0055 0.0132 0.0087 0.0007 0.0006 0.022 0.0027 0.0076 0.0016 0.0189 0.0043 0.0139 0.0097 0.0084 0.0192 0.0076 0.0112
Punctate Nuclear 0.0034 0 0.0001 0.0017 0 0 0.0025 0 0.0004 0 0.0004 0.0013 0.0005 0 0.0001 0.0013 0.0007 0
Vacuole 0.0066 0.0042 0.0026 0.0074 0.0002 0.0024 0.0026 0.0005 0.0014 0.0001 0.0014 0.0012 0.0022 0.0021 0.0007 0.0005 0.002 0.0007
Vacuole Periphery 0.0059 0.0079 0.0064 0.0088 0.0012 0.0092 0.0045 0.0014 0.0028 0.0004 0.003 0.0101 0.0046 0.0021 0.0022 0.0008 0.0025 0.0042

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9027 15.3096 17.852 26.0467 18.5039 18.7467 26.9049 26.6166 25.1904 27.4018
Translational Efficiency 1.3296 1.4672 1.2448 0.8546 1.0805 1.4259 0.9922 1.072 1.2157 1.3012

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘