Standard name
Description Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0.09 0.07 0 0.1 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.63 0.72 0.8 0.92 0.92 0.84 0.87 – 0.65 0.79 0.57 0.64 – – – 0.7 0.57 0.57 0.39 0.5 0.48 0.47 0.22 0.42
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.05 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.08 0.1 – 0.52 0.22 0.52 0.39 – – – 0 0.05 0 0 0 0.06 0 0 0
Nucleus 0.24 0.18 0.15 0 0 0.05 0 – 0 0.06 0 0 – – – 0.06 0.08 0.1 0.23 0.22 0.15 0.18 0.31 0.08
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0.06 0.06 0.07 0.08 0 0.07
Vac/Vac Membrane 0.13 0.12 0.05 0 0 0 0 – 0 0 0 0 – – – 0.16 0.28 0.3 0.11 0.08 0.12 0.1 0 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 1 0 – – – 1 0 0 1 2 1 0 0 2
Bud 0 0 0 0 0 0 0 – 3 0 3 1 – – – 0 1 0 18 21 3 1 0 0
Bud Neck 0 1 1 0 1 0 2 – 1 6 3 4 – – – 0 1 0 3 2 1 0 0 0
Bud Site 0 0 0 0 0 0 0 – 1 0 1 1 – – – 0 0 0 – – – – – –
Cell Periphery 2 1 1 0 2 1 0 – 0 0 0 0 – – – 1 2 0 0 0 0 0 0 0
Cytoplasm 113 207 67 77 47 80 104 – 57 115 82 83 – – – 44 74 52 80 155 54 7 2 15
Endoplasmic Reticulum 1 0 0 1 1 0 0 – 0 2 3 2 – – – 3 9 6 0 0 0 0 0 0
Endosome 0 4 1 1 0 2 0 – 0 0 0 0 – – – 2 5 1 2 4 1 0 0 2
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 – – – 1 2 3 3 0 0 0 0 0
Mitochondria 13 4 0 3 0 8 12 – 46 32 76 50 – – – 2 7 2 7 6 6 0 0 1
Nucleus 44 53 13 2 2 5 4 – 3 9 6 5 – – – 4 11 9 46 67 17 2 4 2
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 3 0 – 0 2 2 1 – – – 1 1 0 11 19 8 1 0 2
Vac/Vac Membrane 23 36 4 3 2 3 2 – 2 2 1 0 – – – 10 36 27 21 26 13 1 0 3
Unique Cell Count 180 289 84 84 51 95 120 88 146 145 129 63 130 91 204 313 113 16 13 37
Labelled Cell Count 196 308 87 87 55 102 124 113 169 178 147 70 150 100 204 313 113 16 13 37


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.8 5.4 4.9 5.9 4.1 4.4 – 3.3 3.8 3.2 3.3 – – – 6.2 6.6 6.6 – – –
Std Deviation (1e-4) 1.0 0.9 1.1 1.4 2.7 1.7 2.0 – 0.8 1.4 1.0 0.4 – – – 2.0 2.1 2.2 – – –
Intensity Change (Log2) – – – -0.14 0.12 -0.4 -0.31 – -0.7 -0.51 -0.77 -0.7 – – – 0.19 0.29 0.28 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.3 4.2 4.3