Standard name | |
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Human Ortholog | |
Description | Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | – | – | – | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 3 | 1 | – | – | – | 0 | 1 | 0 | 18 | 21 | 3 | 1 | 0 | 0 |
Bud Neck | 0 | 1 | 1 | 0 | 1 | 0 | 2 | – | 1 | 6 | 3 | 4 | – | – | – | 0 | 1 | 0 | 3 | 2 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 1 | 0 | 2 | 1 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 113 | 207 | 67 | 77 | 47 | 80 | 104 | – | 57 | 115 | 82 | 83 | – | – | – | 44 | 74 | 52 | 80 | 155 | 54 | 7 | 2 | 15 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 2 | 3 | 2 | – | – | – | 3 | 9 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4 | 1 | 1 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 2 | 5 | 1 | 2 | 4 | 1 | 0 | 0 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 13 | 4 | 0 | 3 | 0 | 8 | 12 | – | 46 | 32 | 76 | 50 | – | – | – | 2 | 7 | 2 | 7 | 6 | 6 | 0 | 0 | 1 |
Nucleus | 44 | 53 | 13 | 2 | 2 | 5 | 4 | – | 3 | 9 | 6 | 5 | – | – | – | 4 | 11 | 9 | 46 | 67 | 17 | 2 | 4 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 3 | 0 | – | 0 | 2 | 2 | 1 | – | – | – | 1 | 1 | 0 | 11 | 19 | 8 | 1 | 0 | 2 |
Vac/Vac Membrane | 23 | 36 | 4 | 3 | 2 | 3 | 2 | – | 2 | 2 | 1 | 0 | – | – | – | 10 | 36 | 27 | 21 | 26 | 13 | 1 | 0 | 3 |
Unique Cell Count | 180 | 289 | 84 | 84 | 51 | 95 | 120 | 88 | 146 | 145 | 129 | 63 | 130 | 91 | 204 | 313 | 113 | 16 | 13 | 37 | ||||
Labelled Cell Count | 196 | 308 | 87 | 87 | 55 | 102 | 124 | 113 | 169 | 178 | 147 | 70 | 150 | 100 | 204 | 313 | 113 | 16 | 13 | 37 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 4.8 | 5.4 | 4.9 | 5.9 | 4.1 | 4.4 | – | 3.3 | 3.8 | 3.2 | 3.3 | – | – | – | 6.2 | 6.6 | 6.6 | – | – | – |
Std Deviation (1e-4) | 1.0 | 0.9 | 1.1 | 1.4 | 2.7 | 1.7 | 2.0 | – | 0.8 | 1.4 | 1.0 | 0.4 | – | – | – | 2.0 | 2.1 | 2.2 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.14 | 0.12 | -0.4 | -0.31 | – | -0.7 | -0.51 | -0.77 | -0.7 | – | – | – | 0.19 | 0.29 | 0.28 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.3 | 4.2 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4175 | -0.581 | -0.6322 | -0.1948 | 1.3251 | -0.2543 | 2.3077 | -0.7002 | -0.22 | -0.3195 | 0.9932 | -0.5445 | 4.7745 | -0.5942 | -0.5022 | -0.2096 | 0.8885 | -0.6606 |
Actin | 0.2209 | 0.0004 | 0.0041 | 0.0123 | 0.067 | 0.0131 | 0.1459 | 0.0003 | 0.0206 | 0.0003 | 0.0229 | 0.0189 | 0.209 | 0.0006 | 0.0236 | 0.0019 | 0.0496 | 0.0003 |
Bud | 0.0048 | 0.0063 | 0.0059 | 0.0009 | 0.006 | 0.0039 | 0.0032 | 0.0015 | 0.0059 | 0 | 0.0009 | 0.0011 | 0.0141 | 0.002 | 0.0027 | 0.0003 | 0.002 | 0.0009 |
Bud Neck | 0.0115 | 0.0004 | 0.002 | 0.0008 | 0.0039 | 0.0033 | 0.0099 | 0.0005 | 0.0083 | 0.0004 | 0.0157 | 0.0077 | 0.0312 | 0.0008 | 0.0011 | 0.0006 | 0.004 | 0.0031 |
Bud Periphery | 0.022 | 0.0024 | 0.0084 | 0.0012 | 0.0103 | 0.007 | 0.0085 | 0.002 | 0.0112 | 0 | 0.0017 | 0.002 | 0.0292 | 0.0023 | 0.0026 | 0.0003 | 0.0039 | 0.0015 |
Bud Site | 0.0186 | 0.011 | 0.017 | 0.0004 | 0.0286 | 0.0003 | 0.0254 | 0.0055 | 0.0102 | 0.0001 | 0.003 | 0.0006 | 0.0451 | 0.005 | 0.0098 | 0.0002 | 0.0027 | 0.0002 |
Cell Periphery | 0.002 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0013 | 0.0005 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0041 | 0.0007 | 0.0014 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0312 | 0.0396 | 0.0482 | 0.0578 | 0.0163 | 0.0814 | 0.0326 | 0.0536 | 0.0323 | 0.0415 | 0.0361 | 0.0627 | 0.0219 | 0.0931 | 0.1372 | 0.1036 | 0.0095 | 0.2019 |
Cytoplasmic Foci | 0.06 | 0.0051 | 0.0104 | 0.0037 | 0.0285 | 0.0059 | 0.0671 | 0.0042 | 0.0179 | 0.0017 | 0.0314 | 0.0029 | 0.0414 | 0.0021 | 0.009 | 0.0065 | 0.0103 | 0.0034 |
Eisosomes | 0.0022 | 0.0001 | 0.0006 | 0.0002 | 0.0011 | 0.0002 | 0.0017 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0013 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0359 | 0.0011 | 0.0026 | 0.0024 | 0.007 | 0.0016 | 0.0142 | 0.001 | 0.0028 | 0.0007 | 0.0049 | 0.0014 | 0.0141 | 0.0022 | 0.0065 | 0.0011 | 0.0004 | 0.0016 |
Endosome | 0.0452 | 0.0013 | 0.0036 | 0.0053 | 0.0333 | 0.0074 | 0.0709 | 0.0005 | 0.0051 | 0.0012 | 0.0379 | 0.0068 | 0.1463 | 0.0013 | 0.0031 | 0.0237 | 0.0104 | 0.0015 |
Golgi | 0.0273 | 0.0001 | 0.0014 | 0.0012 | 0.0228 | 0.0031 | 0.0143 | 0 | 0.0032 | 0 | 0.0113 | 0.0021 | 0.0441 | 0.0001 | 0.0011 | 0.0086 | 0.0291 | 0 |
Lipid Particles | 0.0607 | 0.0004 | 0.0053 | 0.0046 | 0.0435 | 0.0102 | 0.04 | 0.0002 | 0.005 | 0.0002 | 0.0375 | 0.0032 | 0.0537 | 0.0002 | 0.0015 | 0.0018 | 0.0098 | 0.0001 |
Mitochondria | 0.0431 | 0.0031 | 0.0022 | 0.0011 | 0.0237 | 0.0085 | 0.0844 | 0.0006 | 0.0018 | 0.0008 | 0.0118 | 0.0024 | 0.0323 | 0.0009 | 0.0009 | 0.0049 | 0.0126 | 0.0007 |
None | 0.1895 | 0.7418 | 0.754 | 0.269 | 0.0857 | 0.6911 | 0.3172 | 0.8231 | 0.7644 | 0.4099 | 0.1653 | 0.7502 | 0.0648 | 0.7261 | 0.6768 | 0.2241 | 0.0961 | 0.5253 |
Nuclear Periphery | 0.0306 | 0.0049 | 0.0078 | 0.0248 | 0.0272 | 0.0106 | 0.034 | 0.002 | 0.0039 | 0.02 | 0.0315 | 0.0076 | 0.0524 | 0.0048 | 0.0074 | 0.009 | 0.0028 | 0.0035 |
Nucleolus | 0.0044 | 0.0037 | 0.0028 | 0.0053 | 0.01 | 0.004 | 0.0074 | 0.0018 | 0.0032 | 0.0047 | 0.0092 | 0.0034 | 0.0246 | 0.0011 | 0.0012 | 0.0024 | 0.0097 | 0.0041 |
Nucleus | 0.0954 | 0.1578 | 0.1004 | 0.559 | 0.436 | 0.107 | 0.0258 | 0.099 | 0.0473 | 0.4887 | 0.4843 | 0.1073 | 0.0308 | 0.1441 | 0.0827 | 0.5787 | 0.6971 | 0.2432 |
Peroxisomes | 0.0447 | 0.0004 | 0.0067 | 0.0011 | 0.0407 | 0.0061 | 0.0239 | 0.0004 | 0.029 | 0.0001 | 0.0291 | 0.0026 | 0.0154 | 0.0001 | 0.0082 | 0.0007 | 0.0081 | 0.0001 |
Punctate Nuclear | 0.0121 | 0.0185 | 0.0138 | 0.0469 | 0.1014 | 0.033 | 0.0629 | 0.0021 | 0.0244 | 0.0286 | 0.0574 | 0.0158 | 0.0648 | 0.0028 | 0.0192 | 0.0301 | 0.0388 | 0.0058 |
Vacuole | 0.026 | 0.0011 | 0.0016 | 0.0012 | 0.0043 | 0.0011 | 0.0063 | 0.0012 | 0.0024 | 0.0006 | 0.0048 | 0.0006 | 0.0502 | 0.0077 | 0.0035 | 0.001 | 0.002 | 0.0023 |
Vacuole Periphery | 0.0118 | 0.0002 | 0.0005 | 0.0007 | 0.0021 | 0.0009 | 0.003 | 0.0002 | 0.0004 | 0.0003 | 0.0024 | 0.0005 | 0.0093 | 0.0019 | 0.0005 | 0.0005 | 0.0006 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.3108 | 8.6099 | 28.7503 | 26.9866 | 8.195 | 9.0245 | 20.1315 | 36.7227 | 22.4277 | 9.8571 |
Translational Efficiency | 0.621 | 1.709 | 1.3587 | 0.8524 | 0.775 | 0.289 | 1.2131 | 0.9034 | 1.3122 | 1.0164 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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