Standard name | |
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Description | Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | – | – | – | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 3 | 1 | – | – | – | 0 | 1 | 0 | 18 | 21 | 3 | 1 | 0 | 0 |
Bud Neck | 0 | 1 | 1 | 0 | 1 | 0 | 2 | – | 1 | 6 | 3 | 4 | – | – | – | 0 | 1 | 0 | 3 | 2 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 1 | 0 | 2 | 1 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 113 | 207 | 67 | 77 | 47 | 80 | 104 | – | 57 | 115 | 82 | 83 | – | – | – | 44 | 74 | 52 | 80 | 155 | 54 | 7 | 2 | 15 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 2 | 3 | 2 | – | – | – | 3 | 9 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4 | 1 | 1 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 2 | 5 | 1 | 2 | 4 | 1 | 0 | 0 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 13 | 4 | 0 | 3 | 0 | 8 | 12 | – | 46 | 32 | 76 | 50 | – | – | – | 2 | 7 | 2 | 7 | 6 | 6 | 0 | 0 | 1 |
Nucleus | 44 | 53 | 13 | 2 | 2 | 5 | 4 | – | 3 | 9 | 6 | 5 | – | – | – | 4 | 11 | 9 | 46 | 67 | 17 | 2 | 4 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 3 | 0 | – | 0 | 2 | 2 | 1 | – | – | – | 1 | 1 | 0 | 11 | 19 | 8 | 1 | 0 | 2 |
Vac/Vac Membrane | 23 | 36 | 4 | 3 | 2 | 3 | 2 | – | 2 | 2 | 1 | 0 | – | – | – | 10 | 36 | 27 | 21 | 26 | 13 | 1 | 0 | 3 |
Unique Cell Count | 180 | 289 | 84 | 84 | 51 | 95 | 120 | 88 | 146 | 145 | 129 | 63 | 130 | 91 | 204 | 313 | 113 | 16 | 13 | 37 | ||||
Labelled Cell Count | 196 | 308 | 87 | 87 | 55 | 102 | 124 | 113 | 169 | 178 | 147 | 70 | 150 | 100 | 204 | 313 | 113 | 16 | 13 | 37 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 4.8 | 5.4 | 4.9 | 5.9 | 4.1 | 4.4 | – | 3.3 | 3.8 | 3.2 | 3.3 | – | – | – | 6.2 | 6.6 | 6.6 | – | – | – |
Std Deviation (1e-4) | 1.0 | 0.9 | 1.1 | 1.4 | 2.7 | 1.7 | 2.0 | – | 0.8 | 1.4 | 1.0 | 0.4 | – | – | – | 2.0 | 2.1 | 2.2 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.14 | 0.12 | -0.4 | -0.31 | – | -0.7 | -0.51 | -0.77 | -0.7 | – | – | – | 0.19 | 0.29 | 0.28 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.3 | 4.2 | 4.3 |