Standard name
Human Ortholog
Description Subunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.06 0 0.06 0.06 0.1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.06 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0 0.08 0.12 0.13 0.14 0.12 0.13 0.17 0.2 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.9 0.86 0.88 0.88 0.85 0.77 0.75 0.74 0.74 0.71 0.53 0.58 0.89 0.8 0.75 0.82 0.8 0.75 0.83 0.8 0.81 0.74 0.67 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.45 0.38 0.39 0.28 0.29 0.39 0.48 0.5 0.47 0.59 0.72 0.67 0.3 0.38 0.31 0.18 0.17 0.25 0.13 0.13 0.13 0.19 0.24 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 0 0 3 2 1 0 1 3
Bud Neck 0 1 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 1 1 4 1 9 3 7 4 11 2 3 3 2 0 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 10 1 2 0 1 3 2 4 3 1 1 1 12 30 33 23 27 20 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 6 4 0 0 0 0 0 1
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 1 2 1 2 3 1 2 0 0 3 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Mitochondria 5 4 8 3 6 14 18 22 12 15 11 22 0 2 1 17 1 10 0 0 0 1 0 3
Nucleus 393 167 100 69 62 90 107 116 75 82 34 64 318 339 276 299 262 190 362 167 94 176 206 249
Nuclear Periphery 0 0 0 0 0 0 0 3 2 2 1 0 2 1 1 6 1 2 0 1 0 0 0 2
Nucleolus 198 73 44 22 21 46 69 78 47 68 46 74 107 161 113 67 57 64 58 27 15 45 74 92
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 4 5 2 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 1 0 0 0 1 4 9 8 4 1 3 1 0 0 1 1 5
Vac/Vac Membrane 1 0 0 0 0 1 0 0 0 1 1 0 0 2 6 1 3 6 6 8 2 7 16 24
Unique Cell Count 436 194 114 78 73 117 143 156 101 115 64 111 357 425 369 364 329 253 438 211 117 238 308 392
Labelled Cell Count 609 248 154 96 91 158 197 234 143 177 98 173 447 553 447 432 361 304 438 211 117 238 308 392


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 12.0 8.4 8.6 9.0 7.5 7.2 6.3 7.4 7.0 6.5 7.0 11.2 11.1 10.7 10.8 14.5 12.2 8.5 8.9 9.3
Std Deviation (1e-4) 3.1 2.9 2.2 2.4 4.0 2.4 2.6 2.0 2.2 2.3 2.1 2.0 2.9 3.2 3.4 4.2 4.5 4.9 2.2 1.9 1.9
Intensity Change (Log2) – – – 0.02 0.09 -0.17 -0.22 -0.43 -0.2 -0.28 -0.38 -0.27 0.4 0.4 0.34 0.35 0.78 0.53 0.01 0.08 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 2.1 2.6 1.9 2.4 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 1.3 1.5 1.8 1.2 1.5 2.1 2.8 0 0 0 -1.0 0 -1.3
Nucleus 0.2 -0.5 -2.1 -2.6 -2.7 -2.5 -3.1 -5.1 -5.1 0.4 -1.9 -2.9 -1.4 -1.9 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -1.4 0.1 1.5 1.9 1.2 3.1 4.3 4.2 -1.7 -0.1 -1.6 -4.5 -4.7 -2.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0303 0.0113 0.0001 0 0.0002 0 0.0038 0 0.0223 0 0.0001 0 0.0583 0.0002 0.0434 0.0004 0.0338 0.0002
Bud 0.0012 0.0085 0.0001 0 0.0002 0.0001 0.0005 0 0.0002 0 0.0003 0 0.0025 0.0001 0.0001 0.0001 0.0013 0.0001
Bud Neck 0.0007 0.0028 0.0001 0.0001 0.0009 0.0005 0.0003 0 0.0002 0 0.0003 0.0003 0.0077 0 0.0021 0.0001 0.0003 0.0003
Bud Periphery 0.0013 0.0062 0.0001 0.0001 0.001 0.0002 0.0006 0 0.0004 0 0.0012 0 0.0041 0.0003 0.0002 0.0001 0.0039 0.0001
Bud Site 0.0103 0.0732 0.0002 0 0.0002 0.0001 0.0011 0 0.0004 0 0.0005 0 0.0455 0.0002 0.0018 0.0003 0.0007 0.0001
Cell Periphery 0.0004 0.0017 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0001 0 0.0002 0 0.0019 0.0001 0.0003 0 0.0002 0.0001
Cytoplasm 0.0074 0.0147 0.0004 0 0.0001 0 0.0039 0 0.0009 0 0 0.0011 0.0059 0.0001 0.0015 0.0004 0.0014 0.0002
Cytoplasmic Foci 0.1389 0.0178 0.0046 0 0 0 0.01 0 0.0034 0 0.0001 0.0022 0.0551 0.0003 0.0041 0.0068 0.0035 0.0005
Eisosomes 0.0008 0.0002 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0006 0 0.0003 0 0.0003 0
Endoplasmic Reticulum 0.0057 0.0068 0.0001 0 0.0001 0 0.0015 0 0.0079 0 0 0.0001 0.0021 0 0.0006 0.0001 0.0063 0.0006
Endosome 0.0892 0.0064 0.0004 0.0001 0.0002 0 0.0109 0 0.0068 0 0.0001 0.0004 0.0275 0.0001 0.002 0.0037 0.05 0.0007
Golgi 0.0334 0.0021 0.0002 0 0 0 0.002 0 0.005 0 0 0 0.0147 0.0001 0.0021 0.001 0.0208 0.0001
Lipid Particles 0.2836 0.0126 0.0042 0.0001 0.0001 0 0.0083 0 0.0088 0 0.0002 0.0002 0.0427 0.0009 0.0018 0.0072 0.0111 0.0015
Mitochondria 0.0087 0.0028 0.0024 0.0003 0.0035 0.0003 0.0017 0.0001 0.0063 0.0001 0.0024 0.0001 0.0143 0.0212 0.0003 0.0007 0.0247 0.0008
None 0.0278 0.0024 0.0007 0 0.0002 0 0.004 0 0.0005 0 0.0001 0.0006 0.0109 0.0001 0.0008 0.0017 0.0022 0.0002
Nuclear Periphery 0.046 0.0137 0.007 0.0008 0.0028 0.0002 0.021 0.0001 0.0124 0.0001 0.0008 0.0005 0.0134 0.0005 0.0011 0.0015 0.0657 0.005
Nucleolus 0.014 0.0929 0.0779 0.1492 0.2428 0.2151 0.0777 0.0454 0.0366 0.0264 0.2091 0.1725 0.1264 0.04 0.0314 0.06 0.1614 0.1535
Nucleus 0.0089 0.6922 0.8703 0.8468 0.7448 0.7826 0.8013 0.9541 0.8663 0.9732 0.783 0.818 0.3657 0.9256 0.8923 0.8591 0.5932 0.8333
Peroxisomes 0.0471 0.0015 0.0003 0 0.0001 0 0.0065 0 0.002 0 0.0001 0 0.0284 0.0004 0.0029 0.0023 0.0016 0.0001
Punctate Nuclear 0.237 0.0198 0.0291 0.0003 0.0007 0.0002 0.0415 0.0001 0.0159 0.0002 0.0005 0.004 0.1521 0.0072 0.0108 0.0537 0.0106 0.0018
Vacuole 0.0034 0.0086 0.0004 0.0009 0.0009 0.0004 0.0019 0 0.0008 0 0.0006 0.0001 0.011 0.0007 0.0002 0.0003 0.0022 0.0003
Vacuole Periphery 0.0038 0.0019 0.0012 0.001 0.0011 0.0002 0.0012 0 0.0028 0 0.0003 0 0.0093 0.002 0.0001 0.0002 0.0048 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.658 31.1621 24.5394 30.7108 37.8176 29.8629 34.8838 32.9836 28.3177 37.7845
Translational Efficiency 1.1525 1.2031 1.1515 0.8233 0.7143 1.1492 1.014 0.824 0.945 0.9169

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2046 1605 262 1998 757 1789 306 106 2803 3394 568 2104

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 920.32 1188.77 1401.82 1408.82 1149.98 1135.11 1501.53 1442.71 982.34 1160.49 1455.54 1410.53
Standard Deviation 178.59 282.08 251.34 268.40 235.63 229.00 231.36 345.42 220.62 256.88 245.86 272.90
Intensity Change Log 2 — 0.369262 0.607094 0.614280 — -0.018777 0.384825 0.327173 — 0.166696 0.487879 0.461896

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000598 0.000563 0.001195 0.000723 0.000388 0.000566 0.000860 0.001111 0.000541 0.000565 0.001014 0.000743
Bud Neck 0.008539 0.004930 0.011416 0.019483 0.006140 0.005867 0.012903 0.011963 0.007891 0.005424 0.012217 0.019104
Bud Site 0.001342 0.000737 0.001339 0.001936 0.000500 0.000726 0.000923 0.003735 0.001115 0.000732 0.001115 0.002026
Cell Periphery 0.000120 0.000054 0.000032 0.000102 0.000042 0.000073 0.000020 0.000076 0.000099 0.000064 0.000025 0.000100
Cytoplasm 0.000915 0.001610 0.000042 0.000227 0.000022 0.000092 0.000028 0.000683 0.000674 0.000810 0.000035 0.000250
Cytoplasmic Foci 0.000163 0.001310 0.000122 0.000215 0.000036 0.000274 0.000070 0.010260 0.000129 0.000764 0.000094 0.000721
Eisosomes 0.000068 0.000108 0.000139 0.000061 0.000165 0.000103 0.000106 0.000077 0.000094 0.000105 0.000121 0.000061
Endoplasmic Reticulum 0.001859 0.000725 0.000914 0.000785 0.000998 0.000949 0.000546 0.000973 0.001626 0.000843 0.000716 0.000794
Endosome 0.000187 0.000074 0.000027 0.000391 0.000029 0.000374 0.000013 0.012214 0.000144 0.000232 0.000020 0.000987
Golgi 0.000131 0.000056 0.000127 0.000442 0.000024 0.000220 0.000072 0.006290 0.000102 0.000142 0.000097 0.000737
Lipid Particles 0.000152 0.000034 0.000025 0.000030 0.000004 0.000228 0.000012 0.000792 0.000112 0.000136 0.000018 0.000069
Mitochondria 0.000998 0.001148 0.002198 0.002479 0.000679 0.001020 0.001908 0.014539 0.000912 0.001081 0.002042 0.003087
Mitotic Spindle 0.002728 0.001454 0.000343 0.005556 0.000491 0.002544 0.003609 0.007939 0.002124 0.002028 0.002103 0.005676
None 0.006611 0.001768 0.000729 0.001136 0.000439 0.000573 0.000574 0.001574 0.004944 0.001139 0.000646 0.001158
Nuclear Periphery 0.000210 0.000109 0.000067 0.000526 0.000032 0.000144 0.000051 0.000392 0.000162 0.000128 0.000058 0.000519
Nuclear Periphery Foci 0.000355 0.000378 0.000018 0.000155 0.000014 0.000621 0.000008 0.000242 0.000263 0.000506 0.000013 0.000159
Nucleolus 0.096173 0.084512 0.098202 0.029769 0.059436 0.076941 0.069544 0.055198 0.086252 0.080521 0.082763 0.031050
Nucleus 0.877583* 0.897751* 0.873464* 0.933333* 0.929212* 0.905917* 0.904161* 0.848575* 0.891526* 0.902055* 0.890001* 0.929063*
Peroxisomes 0.000941 0.002130 0.008965 0.001041 0.000866 0.001833 0.004162 0.004174 0.000921 0.001974 0.006378 0.001199
Vacuole 0.000144 0.000269 0.000423 0.000888 0.000220 0.000444 0.000278 0.011544 0.000165 0.000361 0.000345 0.001425
Vacuole Periphery 0.000183 0.000279 0.000212 0.000721 0.000264 0.000489 0.000153 0.007648 0.000205 0.000390 0.000180 0.001070

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.12 -8.85 -4.38 -5.26 6.68 -4.08 -10.23 -4.73 -4.09 -2.96 -0.76 -11.46 -7.38 -6.20 5.14
Bud Neck 7.27 -4.28 -9.88 -12.93 -5.97 0.30 -6.65 -5.38 -5.67 -0.95 5.87 -7.25 -10.81 -13.03 -5.58
Bud Site 1.23 -0.81 -1.73 -3.71 -1.49 -1.51 -4.51 -2.89 -2.75 -2.53 1.12 -0.90 -2.95 -4.71 -2.99
Cell Periphery 4.17 5.99 0.92 -2.72 -4.09 -1.39 2.34 -2.68 -0.75 -3.63 2.11 6.72 -0.15 -1.90 -4.83
Cytoplasm -1.14 4.70 3.44 1.94 -5.92 -2.45 -1.05 -2.37 -2.19 -1.31 -0.65 1.91 2.52 1.58 -0.46
Cytoplasmic Foci -1.93 0.67 -0.81 1.81 -1.41 -2.14 -1.56 -1.87 -1.79 -1.86 -2.15 0.73 -2.21 0.89 -2.43
Eisosomes -6.23 -8.77 1.89 7.69 9.80 4.18 3.68 4.56 1.18 1.91 -1.86 -4.93 6.50 9.42 11.96
Endoplasmic Reticulum 12.28 4.34 12.02 -1.64 2.28 0.22 5.87 -2.23 -2.23 -2.98 8.22 8.59 9.71 -0.61 -0.55
Endosome 2.83 7.23 -1.53 -2.45 -2.88 -2.59 1.30 -1.75 -1.66 -1.76 -1.21 7.76 -2.42 -1.92 -2.94
Golgi 3.30 -1.04 -1.15 -1.49 -1.04 -2.27 -6.77 -1.53 -1.44 -1.50 -0.83 -1.14 -1.85 -1.66 -1.77
Lipid Particles 1.64 1.60 1.71 0.17 0.76 -2.28 -4.00 -2.03 -1.13 -1.99 -0.45 1.67 0.97 1.47 -2.07
Mitochondria -0.81 -8.95 -8.71 -6.58 -1.48 -2.30 -5.86 -1.89 -1.81 -1.56 -1.46 -8.75 -7.16 -6.43 -2.41
Mitotic Spindle 1.53 2.42 -1.71 -2.83 -3.47 -2.55 -1.58 -2.42 -2.16 -1.44 0.33 -0.87 -2.89 -3.08 -0.68
None 4.35 6.08 5.55 0.85 -2.19 -1.18 -1.78 -3.50 -3.27 -3.13 5.06 6.02 5.08 -0.44 -3.05
Nuclear Periphery 2.56 4.52 -2.80 -3.48 -3.67 -3.16 -2.77 -4.22 -2.99 -3.62 1.19 3.67 -3.31 -3.53 -3.87
Nuclear Periphery Foci -0.01 3.80 2.22 0.80 -2.70 -2.53 -0.24 -1.77 -0.20 -1.76 -1.23 3.85 1.18 1.76 -3.37
Nucleolus 2.86 -0.19 18.55 14.13 8.00 -3.74 -1.70 -1.25 0.26 -0.16 1.97 0.46 17.23 16.48 9.19
Nucleus -4.06 0.75 -10.67 -6.04 -6.28 4.42 3.86 4.21 3.28 2.72 -2.90 1.18 -7.15 -4.84 -5.76
Peroxisomes -6.62 -6.90 -0.37 3.71 6.71 -2.98 -6.23 -2.83 -2.31 -0.96 -5.97 -8.95 -1.46 2.22 7.84
Vacuole -2.87 -2.12 -3.37 -2.77 -0.13 -2.02 -1.53 -2.43 -2.37 -2.36 -3.42 -2.74 -4.02 -3.31 -2.08
Vacuole Periphery -2.83 -3.36 -2.91 -2.32 -2.65 -1.83 1.98 -1.30 -1.23 -1.33 -2.89 0.56 -2.92 -2.04 -2.96
Standard name
Human Ortholog
Description Subunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Taf6

Taf6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available