Standard name
Description Subunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.06 0 0.06 0.06 0.1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.06 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0 0.08 0.12 0.13 0.14 0.12 0.13 0.17 0.2 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.9 0.86 0.88 0.88 0.85 0.77 0.75 0.74 0.74 0.71 0.53 0.58 0.89 0.8 0.75 0.82 0.8 0.75 0.83 0.8 0.81 0.74 0.67 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.45 0.38 0.39 0.28 0.29 0.39 0.48 0.5 0.47 0.59 0.72 0.67 0.3 0.38 0.31 0.18 0.17 0.25 0.13 0.13 0.13 0.19 0.24 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 0 0 3 2 1 0 1 3
Bud Neck 0 1 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 1 1 4 1 9 3 7 4 11 2 3 3 2 0 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 10 1 2 0 1 3 2 4 3 1 1 1 12 30 33 23 27 20 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 6 4 0 0 0 0 0 1
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 1 2 1 2 3 1 2 0 0 3 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Mitochondria 5 4 8 3 6 14 18 22 12 15 11 22 0 2 1 17 1 10 0 0 0 1 0 3
Nucleus 393 167 100 69 62 90 107 116 75 82 34 64 318 339 276 299 262 190 362 167 94 176 206 249
Nuclear Periphery 0 0 0 0 0 0 0 3 2 2 1 0 2 1 1 6 1 2 0 1 0 0 0 2
Nucleolus 198 73 44 22 21 46 69 78 47 68 46 74 107 161 113 67 57 64 58 27 15 45 74 92
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 4 5 2 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 1 0 0 0 1 4 9 8 4 1 3 1 0 0 1 1 5
Vac/Vac Membrane 1 0 0 0 0 1 0 0 0 1 1 0 0 2 6 1 3 6 6 8 2 7 16 24
Unique Cell Count 436 194 114 78 73 117 143 156 101 115 64 111 357 425 369 364 329 253 438 211 117 238 308 392
Labelled Cell Count 609 248 154 96 91 158 197 234 143 177 98 173 447 553 447 432 361 304 438 211 117 238 308 392


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 12.0 8.4 8.6 9.0 7.5 7.2 6.3 7.4 7.0 6.5 7.0 11.2 11.1 10.7 10.8 14.5 12.2 8.5 8.9 9.3
Std Deviation (1e-4) 3.1 2.9 2.2 2.4 4.0 2.4 2.6 2.0 2.2 2.3 2.1 2.0 2.9 3.2 3.4 4.2 4.5 4.9 2.2 1.9 1.9
Intensity Change (Log2) – – – 0.02 0.09 -0.17 -0.22 -0.43 -0.2 -0.28 -0.38 -0.27 0.4 0.4 0.34 0.35 0.78 0.53 0.01 0.08 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 2.1 2.6 1.9 2.4 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 1.3 1.5 1.8 1.2 1.5 2.1 2.8 0 0 0 -1.0 0 -1.3
Nucleus 0.2 -0.5 -2.1 -2.6 -2.7 -2.5 -3.1 -5.1 -5.1 0.4 -1.9 -2.9 -1.4 -1.9 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -1.4 0.1 1.5 1.9 1.2 3.1 4.3 4.2 -1.7 -0.1 -1.6 -4.5 -4.7 -2.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0