Standard name | |
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Description | Subunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 2 | 1 | 0 | 1 | 3 |
Bud Neck | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 1 | 1 | 4 | 1 | 9 | 3 | 7 | 4 | 11 | 2 | 3 | 3 | 2 | 0 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10 | 1 | 2 | 0 | 1 | 3 | 2 | 4 | 3 | 1 | 1 | 1 | 12 | 30 | 33 | 23 | 27 | 20 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 3 | 1 | 2 | 0 | 0 | 3 | 1 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 5 | 4 | 8 | 3 | 6 | 14 | 18 | 22 | 12 | 15 | 11 | 22 | 0 | 2 | 1 | 17 | 1 | 10 | 0 | 0 | 0 | 1 | 0 | 3 |
Nucleus | 393 | 167 | 100 | 69 | 62 | 90 | 107 | 116 | 75 | 82 | 34 | 64 | 318 | 339 | 276 | 299 | 262 | 190 | 362 | 167 | 94 | 176 | 206 | 249 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 1 | 0 | 2 | 1 | 1 | 6 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 2 |
Nucleolus | 198 | 73 | 44 | 22 | 21 | 46 | 69 | 78 | 47 | 68 | 46 | 74 | 107 | 161 | 113 | 67 | 57 | 64 | 58 | 27 | 15 | 45 | 74 | 92 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 9 | 8 | 4 | 1 | 3 | 1 | 0 | 0 | 1 | 1 | 5 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 6 | 1 | 3 | 6 | 6 | 8 | 2 | 7 | 16 | 24 |
Unique Cell Count | 436 | 194 | 114 | 78 | 73 | 117 | 143 | 156 | 101 | 115 | 64 | 111 | 357 | 425 | 369 | 364 | 329 | 253 | 438 | 211 | 117 | 238 | 308 | 392 |
Labelled Cell Count | 609 | 248 | 154 | 96 | 91 | 158 | 197 | 234 | 143 | 177 | 98 | 173 | 447 | 553 | 447 | 432 | 361 | 304 | 438 | 211 | 117 | 238 | 308 | 392 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.4 | 12.0 | 8.4 | 8.6 | 9.0 | 7.5 | 7.2 | 6.3 | 7.4 | 7.0 | 6.5 | 7.0 | 11.2 | 11.1 | 10.7 | 10.8 | 14.5 | 12.2 | 8.5 | 8.9 | 9.3 |
Std Deviation (1e-4) | 3.1 | 2.9 | 2.2 | 2.4 | 4.0 | 2.4 | 2.6 | 2.0 | 2.2 | 2.3 | 2.1 | 2.0 | 2.9 | 3.2 | 3.4 | 4.2 | 4.5 | 4.9 | 2.2 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.09 | -0.17 | -0.22 | -0.43 | -0.2 | -0.28 | -0.38 | -0.27 | 0.4 | 0.4 | 0.34 | 0.35 | 0.78 | 0.53 | 0.01 | 0.08 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 2.6 | 1.9 | 2.4 | 2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0.3 | 1.3 | 1.5 | 1.8 | 1.2 | 1.5 | 2.1 | 2.8 | 0 | 0 | 0 | -1.0 | 0 | -1.3 |
Nucleus | 0.2 | -0.5 | -2.1 | -2.6 | -2.7 | -2.5 | -3.1 | -5.1 | -5.1 | 0.4 | -1.9 | -2.9 | -1.4 | -1.9 | -2.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.5 | -1.4 | 0.1 | 1.5 | 1.9 | 1.2 | 3.1 | 4.3 | 4.2 | -1.7 | -0.1 | -1.6 | -4.5 | -4.7 | -2.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |