Standard name
Human Ortholog
Description Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Kre28p; modified by sumoylation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.07 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0.1 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0.06 0.05 0 0.07 0.05 0 0.09 0.07 0.1 0.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.14 0 0.08 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0.1 0.16 0.08 0 0.06 0 0.05 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.12 0.11 0 0.11 0.07 0.09 0.13 0.1 0.18 0.13 0 0 0.09 0 0 0 0 0 0 0 0 0
Nucleus 0 0.05 0 0 0 0 0.05 0.05 0 0 0.05 0.06 0.06 0.05 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.41 0.39 0.36 0.44 0.37 0.33 0.4 0.4 0.43 0.35 0.59 0.64 0.61 0.61 0.6 0.56 0 0 0 0 0.06 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.69 0.81 0.9 0.89 0.82 0.85 0.81 0.81 0.83 0.77 0.82 0.72 0.66 0.54 0.84 0.79 0.85 0.94 0.82 0.84 0.79 0.76 0.78
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 1 1 1
Bud 0 6 11 13 19 32 45 32 47 24 39 1 0 1 6 5 7 0 0 0 0 1 4
Bud Neck 2 18 13 10 17 12 26 19 20 22 17 2 1 6 2 0 2 0 0 1 0 2 2
Bud Site 1 11 19 16 33 42 30 44 56 43 68 4 3 13 5 0 11
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 4 4 13 6 14 13 8 5 9 4 14 47 69 23 26 25 0 0 0 0 1 1
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 0 0 0 2 2 1 3 0 0 0 0 1 1
Endosome 0 0 2 4 2 8 5 5 2 1 1 17 22 20 67 53 42 3 1 6 3 21 18
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 2 1 1 4
Mitochondria 5 23 45 16 50 59 84 62 76 76 90 7 12 44 17 6 14 0 0 0 0 3 2
Nucleus 2 9 12 18 21 29 42 22 31 10 33 10 28 23 34 14 14 0 0 1 1 6 3
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2
Nucleolus 34 77 152 183 204 303 292 191 320 185 238 104 291 296 400 200 294 1 0 8 1 21 11
Peroxisomes 1 1 6 5 9 22 14 15 29 5 17 3 7 11 21 13 10 0 0 2 1 9 7
SpindlePole 35 153 354 453 383 699 729 391 658 332 559 126 298 262 553 264 441 74 22 161 57 250 270
Vac/Vac Membrane 0 0 0 1 0 1 1 0 0 1 0 1 2 1 7 2 5 0 0 1 1 2 5
Unique Cell Count 51 189 394 510 468 819 895 482 791 429 679 176 454 483 658 336 521 79 27 193 73 331 347
Labelled Cell Count 80 303 619 732 744 1221 1281 790 1244 710 1066 289 712 748 1138 586 868 79 27 193 73 331 347


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 3.8 3.6 4.1 3.7 3.7 3.7 3.3 3.3 3.4 3.1 5.5 5.3 4.6 6.9 7.3 7.8 4.9 5.1 5.5
Std Deviation (1e-4) 0.8 0.9 0.7 1.0 0.9 1.2 0.9 0.7 0.8 0.9 0.7 1.3 1.2 1.2 1.3 1.8 1.5 0.9 1.4 1.5
Intensity Change (Log2) -0.07 0.12 -0.02 -0.03 -0.02 -0.21 -0.18 -0.16 -0.29 0.53 0.47 0.27 0.87 0.93 1.03 0.37 0.41 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 -3.5 -6.3 0 0 -5.8 -6.5
Bud Site 0 0 0 0 0 0 0 0 0 -1.7 0 0 0 0 -2.5
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.6 3.5 4.6 1.0 2.7 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 4.4 3.1 0 4.6 5.8 4.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -2.8 -4.9 0 -5.5 -5.0 -5.0
Nucleus -1.0 -0.8 -0.2 -0.8 0 -0.1 -0.5 -1.6 0.1 0.4 0.7 0 0.2 -0.3 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 3.5 5.5 0 4.9 4.1 3.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0
SpindlePole 0 0 0 0 0 0 0 0 0 -2.1 -3.9 0 1.0 -0.6 1.2
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3516 1.3903 1.4313 4.0945 0.8275 1.0849 2.4821 2.5317 2.2913 2.3025 1.5849 2.025 0.3868 0.3923 0.4692 0.5993 0.1796 0.0848
Actin 0.0062 0.0002 0.0011 0.0056 0.0232 0.0002 0.0058 0.0002 0.0021 0.0104 0.0048 0.0083 0.0444 0 0.0012 0.0001 0.0014 0
Bud 0.0001 0.0001 0.0002 0 0.0001 0 0.0004 0.0002 0.0001 0.0005 0.0001 0 0.0002 0 0.0001 0 0 0
Bud Neck 0.0004 0.0005 0.0026 0.0002 0.0007 0.0001 0.001 0.0008 0.0006 0.0065 0.0033 0.0002 0.0008 0.0011 0.0016 0.0026 0.0003 0.0001
Bud Periphery 0.0003 0.0001 0.0002 0 0.0003 0 0.0004 0.0002 0.0001 0.0006 0.0002 0.0001 0.0002 0 0.0001 0 0 0
Bud Site 0.0005 0.0042 0.0043 0.0004 0.0004 0 0.0031 0.0068 0.0018 0.002 0.0003 0.0001 0.0011 0.0005 0.0007 0.0001 0.0001 0
Cell Periphery 0.0001 0 0.0002 0 0.0001 0 0.0002 0.0001 0 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0007 0.0034 0.0007 0.0001 0.0002 0 0.0147 0.0003 0.0029 0.0004 0.0003 0.0001 0.0034 0.0001 0.0002 0.0001 0.0004 0
Cytoplasmic Foci 0.0162 0.0067 0.0229 0.0166 0.0188 0.0122 0.0214 0.0211 0.0199 0.0313 0.0674 0.0037 0.0284 0.0189 0.0108 0.0541 0.0362 0.006
Eisosomes 0.0003 0 0 0.0001 0.0004 0 0.0001 0 0 0.0001 0.0001 0 0.0005 0 0 0 0 0
Endoplasmic Reticulum 0.0014 0.0002 0.0003 0 0.0015 0 0.0032 0.0001 0.0007 0.0001 0.0013 0.0002 0.0013 0 0.0001 0 0.0001 0
Endosome 0.0074 0.0006 0.0016 0.002 0.0137 0.0003 0.0295 0.0018 0.0043 0.0051 0.0263 0.005 0.0088 0.0003 0.0011 0.0012 0.0032 0
Golgi 0.0028 0.0003 0.001 0.0052 0.0099 0.0003 0.0049 0.0015 0.0012 0.0057 0.0316 0.004 0.0047 0.0004 0.0009 0.002 0.0021 0.0001
Lipid Particles 0.0203 0.0024 0.0153 0.0354 0.0503 0.0066 0.016 0.0078 0.0026 0.0226 0.0666 0.0045 0.0245 0.0139 0.0066 0.044 0.0603 0.0009
Mitochondria 0.0073 0.0002 0.001 0.0387 0.0099 0.0005 0.0144 0.0005 0.0085 0.0288 0.0303 0.0149 0.0014 0.0001 0.0012 0 0 0
None 0.002 0.0009 0.0005 0.0001 0.0006 0 0.009 0.0017 0.0007 0.001 0.0003 0.0001 0.0122 0.0003 0.0018 0.0003 0.0005 0.0001
Nuclear Periphery 0.0102 0.0011 0.0038 0.0004 0.0055 0.0002 0.0151 0.0006 0.0052 0.001 0.0015 0.0005 0.0025 0.0003 0.0005 0.0013 0.0009 0.0002
Nucleolus 0.0068 0.0335 0.0162 0.0024 0.0124 0.0104 0.0151 0.0749 0.0509 0.0024 0.0068 0.0114 0.0081 0.0218 0.0183 0.002 0.0032 0.0051
Nucleus 0.002 0.009 0.0026 0.0004 0.001 0.002 0.013 0.0399 0.0078 0.0007 0.0007 0.0016 0.0025 0.0199 0.003 0.0006 0.0003 0.001
Peroxisomes 0.0091 0.0004 0.0029 0.016 0.0028 0.0024 0.0042 0.0022 0.0064 0.0181 0.0052 0.0011 0.01 0.0052 0.0012 0.0051 0.0054 0.0002
Punctate Nuclear 0.9036 0.9359 0.9218 0.8762 0.8436 0.9647 0.8256 0.8376 0.8828 0.8621 0.7507 0.944 0.8447 0.9168 0.9503 0.8867 0.8853 0.9863
Vacuole 0.0009 0.0002 0.0005 0.0001 0.0012 0 0.0017 0.0014 0.0007 0.0003 0.0006 0.0002 0.0002 0.0002 0.0001 0 0.0001 0
Vacuole Periphery 0.0015 0 0.0002 0.0001 0.0035 0 0.0013 0.0002 0.0005 0.0003 0.0015 0.0002 0.0002 0.0001 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.0377 15.7591 7.4416 10.3667 18.4411 18.0195 19.827 14.4206 13.9705 22.4724
Translational Efficiency 1.3176 1.2882 0.7697 0.8563 1.0629 1.5061 0.8153 0.8459 0.7575 0.7781

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1618 336 352 585 683 999 426 356 2301 1335 778 941

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 612.30 796.49 958.16 871.62 571.39 760.63 933.09 898.80 600.16 769.66 944.43 881.90
Standard Deviation 75.00 118.42 107.26 125.19 106.04 103.82 112.75 125.00 87.42 108.80 111.01 125.81
Intensity Change Log 2 0.379418 0.646028 0.509461 0.412719 0.707540 0.653524 0.395589 0.676049 0.580800

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000774 0.002815 0.000244 0.000206 0.000595 0.000482 0.000155 0.000093 0.000721 0.001069 0.000195 0.000163
Bud Neck 0.011475 0.003751 0.001562 0.003735 0.005039 0.006675 0.000445 0.001192 0.009564 0.005939 0.000951 0.002773
Bud Site 0.013215 0.005437 0.002029 0.008288 0.006508 0.008935 0.000705 0.006021 0.011224 0.008054 0.001304 0.007430
Cell Periphery 0.000529 0.000165 0.000037 0.000095 0.000478 0.000153 0.000017 0.000020 0.000514 0.000156 0.000026 0.000067
Cytoplasm 0.055065 0.013416 0.000813 0.028602 0.041071 0.018569 0.000599 0.002214 0.050911 0.017272 0.000696 0.018619
Cytoplasmic Foci 0.215669* 0.038161 0.002882 0.006266 0.129793 0.044251 0.000429 0.002983 0.190178* 0.042719 0.001539 0.005024
Eisosomes 0.000160 0.000085 0.000026 0.000019 0.000193 0.000087 0.000005 0.000008 0.000170 0.000086 0.000014 0.000015
Endoplasmic Reticulum 0.002202 0.001883 0.000874 0.000583 0.002448 0.001820 0.000134 0.000223 0.002275 0.001836 0.000468 0.000447
Endosome 0.019204 0.007567 0.000788 0.001441 0.017253 0.015861 0.000251 0.000447 0.018625 0.013773 0.000494 0.001065
Golgi 0.030259 0.018870 0.000843 0.000605 0.028791 0.011027 0.000324 0.000366 0.029823 0.013001 0.000559 0.000514
Lipid Particles 0.030634 0.008108 0.000546 0.001483 0.025276 0.012236 0.000137 0.000274 0.029044 0.011197 0.000322 0.001026
Mitochondria 0.003152 0.004603 0.000114 0.000277 0.003268 0.004263 0.000419 0.000093 0.003187 0.004348 0.000281 0.000208
Mitotic Spindle 0.171718* 0.712626* 0.926116* 0.890792* 0.301671* 0.674375* 0.983457* 0.966338* 0.210292* 0.684002* 0.957513* 0.919373*
None 0.063113 0.004432 0.000247 0.003929 0.067017 0.003722 0.000318 0.000151 0.064272 0.003901 0.000286 0.002500
Nuclear Periphery 0.000379 0.000540 0.000257 0.000984 0.000483 0.000551 0.000078 0.000146 0.000410 0.000548 0.000159 0.000667
Nuclear Periphery Foci 0.166318 0.069774 0.019976 0.006334 0.155305 0.070252 0.003170 0.005001 0.163049 0.070132 0.010774 0.005830
Nucleolus 0.107063 0.037183 0.016287 0.014554 0.122158 0.069487 0.004776 0.007013 0.111543 0.061357 0.009984 0.011701
Nucleus 0.028664 0.048851 0.019705 0.026450 0.053856 0.027213 0.003203 0.004249 0.036142 0.032659 0.010669 0.018051
Peroxisomes 0.078175 0.018791 0.003945 0.002346 0.036951 0.023694 0.000840 0.002656 0.065938 0.022460 0.002245 0.002463
Vacuole 0.001934 0.002233 0.002644 0.002864 0.001564 0.004804 0.000428 0.000465 0.001824 0.004157 0.001430 0.001956
Vacuole Periphery 0.000296 0.000712 0.000066 0.000148 0.000284 0.001542 0.000108 0.000046 0.000293 0.001333 0.000089 0.000109

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.08 -2.41 -0.78 1.01 1.98 1.71 1.75 1.72 0.34 -0.38 -0.64 -0.73 0.40 0.74 0.94
Bud Neck 0.21 -0.31 -2.83 -3.46 -2.98 -1.45 1.24 -1.67 -0.56 -2.46 -0.18 0.87 -3.01 -3.32 -4.28
Bud Site -0.11 -0.52 -3.40 -3.02 -2.87 -1.21 1.94 -1.91 -1.38 -2.54 0.17 1.73 -3.72 -3.61 -4.38
Cell Periphery 7.24 11.15 9.14 1.40 -0.68 7.09 10.18 9.98 6.80 -0.62 11.92 16.26 13.38 3.23 -1.69
Cytoplasm 4.28 11.18 0.33 -2.61 -5.07 4.08 6.84 5.17 1.60 -1.42 6.85 12.40 2.24 -2.37 -5.13
Cytoplasmic Foci 16.20 30.29 29.29 6.62 -1.63 9.08 16.20 15.17 10.70 -2.08 21.70 34.35 32.74 12.64 -3.19
Eisosomes 10.83 15.92 26.58 8.21 3.71 5.24 14.14 13.73 17.10 -1.02 13.12 25.48 29.95 17.97 2.03
Endoplasmic Reticulum 0.11 -0.82 3.40 2.27 2.41 1.92 6.52 5.26 4.11 -1.05 2.27 3.03 5.78 3.57 1.11
Endosome 5.87 14.71 14.21 5.27 -0.35 1.18 6.87 6.61 8.07 -0.84 3.97 16.43 15.38 8.70 -1.33
Golgi 6.00 23.81 25.36 3.97 3.64 6.68 11.72 11.67 13.79 -0.47 13.31 26.66 27.20 11.36 2.15
Lipid Particles 7.19 13.35 12.99 4.50 0.28 4.70 9.21 9.14 8.76 -1.10 8.84 16.36 15.90 9.09 -0.41
Mitochondria -0.51 7.31 5.60 1.40 -3.69 0.65 3.80 5.34 2.80 0.32 -0.17 6.20 7.82 2.87 -0.72
Mitotic Spindle -20.29* -33.40* -38.74* -6.80 0.22 -15.77* -34.30* -28.70* -15.33 2.84 -31.08 -54.47 -49.50 -14.87 3.01
None 10.85 13.27 9.99 -0.00 -2.51 7.95 8.75 8.77 6.33 0.12 14.01 15.86 13.54 0.98 -2.47
Nuclear Periphery -2.94 -3.29 -2.40 -1.88 -1.73 -2.10 2.46 -1.30 -0.48 -2.52 -5.09 -0.71 -2.64 -1.94 -2.45
Nuclear Periphery Foci 9.76 19.79 27.63* 8.74 4.11 6.76 18.26 18.04 15.99 -0.19 12.52 29.64 33.26 17.99 3.05
Nucleolus 7.64 10.80 16.21 3.60 2.42 4.42 13.21 11.32 8.48 -1.90 7.74 19.04 19.98 9.40 0.19
Nucleus -2.76 -3.29 -5.87 -1.85 -1.55 2.62 2.81 1.43 -0.53 -1.08 -1.07 -1.45 -4.74 -3.73 -2.71
Peroxisomes 9.26 17.24 21.02 6.49 3.38 4.67 12.26 11.56 9.78 -1.30 11.94 22.51 23.89 11.95 1.71
Vacuole -2.52 -3.15 -3.57 -2.95 -0.88 -2.84 -1.92 -2.49 -2.12 -1.94 -3.61 -3.68 -4.33 -3.65 -2.25
Vacuole Periphery -1.34 -2.35 -3.00 0.75 -0.65 -1.94 0.38 1.64 2.20 0.33 -2.32 -0.58 -1.89 2.02 -0.44