Standard name | |
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Human Ortholog | |
Description | Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 0 | 0 | 4 | 0 | 1 | 4 | 5 | 8 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | – | 5 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 5 | 1 | 3 | 4 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 1 | – | 9 | 3 | 2 | 0 | 3 | 2 | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 4 | 11 | 6 | 8 | 8 | 5 | 9 | – | 5 | 4 | 1 | 20 | 23 | 38 | 24 | 39 | 24 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 12 | 0 | 2 | 1 | 3 | 4 | 5 | 5 | – | 5 | 3 | 4 | 2 | 0 | 2 | 8 | 8 | 5 | 0 | 0 | 2 | 0 | 0 | 1 |
Endosome | 1 | 0 | 11 | 2 | 17 | 6 | 7 | 8 | – | 3 | 1 | 4 | 1 | 0 | 4 | 1 | 6 | 11 | 5 | 7 | 4 | 5 | 25 | 18 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 3 | 1 | 1 | 5 | 7 | 10 | 9 |
Mitochondria | 10 | 45 | 76 | 39 | 39 | 206 | 204 | 187 | – | 315 | 210 | 228 | 3 | 0 | 2 | 24 | 20 | 13 | 7 | 14 | 14 | 40 | 95 | 83 |
Nucleus | 16 | 23 | 6 | 22 | 10 | 18 | 23 | 25 | – | 32 | 23 | 12 | 5 | 4 | 9 | 26 | 51 | 19 | 4 | 7 | 2 | 3 | 5 | 2 |
Nuclear Periphery | 402 | 353 | 322 | 187 | 240 | 259 | 330 | 321 | – | 297 | 178 | 274 | 214 | 118 | 200 | 111 | 144 | 98 | 501 | 392 | 379 | 322 | 359 | 314 |
Nucleolus | 58 | 44 | 101 | 66 | 85 | 125 | 158 | 200 | – | 202 | 124 | 175 | 19 | 12 | 30 | 36 | 36 | 29 | 11 | 12 | 8 | 8 | 10 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | – | 1 | 1 | 3 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 8 | 6 |
SpindlePole | 1 | 0 | 2 | 2 | 0 | 5 | 3 | 1 | – | 2 | 1 | 3 | 7 | 7 | 13 | 1 | 1 | 1 | 2 | 2 | 3 | 2 | 2 | 2 |
Vac/Vac Membrane | 152 | 99 | 61 | 18 | 48 | 21 | 33 | 26 | – | 28 | 15 | 19 | 45 | 33 | 79 | 62 | 57 | 45 | 21 | 25 | 9 | 10 | 22 | 17 |
Unique Cell Count | 501 | 432 | 413 | 253 | 322 | 438 | 525 | 546 | 581 | 363 | 466 | 272 | 157 | 302 | 210 | 288 | 195 | 573 | 473 | 438 | 417 | 555 | 480 | |
Labelled Cell Count | 665 | 568 | 592 | 343 | 451 | 658 | 770 | 786 | 904 | 564 | 729 | 319 | 202 | 384 | 296 | 367 | 249 | 573 | 473 | 438 | 417 | 555 | 480 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.2 | 14.0 | 12.1 | 10.7 | 10.7 | 8.5 | 9.0 | 8.9 | – | 7.5 | 7.6 | 8.4 | 13.1 | 12.5 | 13.0 | 10.2 | 9.8 | 12.6 | 14.3 | 14.8 | 15.6 |
Std Deviation (1e-4) | 5.6 | 3.7 | 3.5 | 3.2 | 3.5 | 2.8 | 3.2 | 3.3 | – | 2.9 | 2.9 | 3.6 | 4.4 | 4.2 | 4.9 | 4.4 | 4.9 | 5.8 | 3.2 | 3.4 | 4.0 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.18 | -0.52 | -0.43 | -0.46 | – | -0.7 | -0.68 | -0.54 | 0.11 | 0.05 | 0.1 | -0.26 | -0.31 | 0.06 | 0.24 | 0.29 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.2 | -0.2 | -0.8 | -2.0 | -1.1 | – | -2.2 | -1.6 | -3.1 | 2.9 | 5.4 | 5.2 | 4.5 | 5.5 | 4.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.8 | -1.3 | -1.5 | -1.3 | – | -2.8 | -2.7 | -2.1 | 0 | 0 | -1.2 | 0 | -0.5 | 1.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.0 | -2.3 | 8.9 | 6.8 | 5.4 | – | 11.4 | 11.4 | 9.5 | -6.9 | -5.8 | -7.5 | -2.2 | -4.3 | -3.8 |
Nucleus | 4.5 | 1.5 | 2.3 | 2.6 | 2.7 | – | 3.3 | 3.6 | 1.2 | 0 | 0 | 1.4 | 5.8 | 7.7 | 4.8 |
Nuclear Periphery | -1.2 | -1.1 | -5.9 | -5.0 | -6.3 | – | -8.6 | -8.4 | -6.1 | 0.2 | -0.7 | -3.5 | -6.4 | -7.7 | -6.9 |
Nucleolus | 0.5 | 0.6 | 1.3 | 1.9 | 4.0 | – | 3.5 | 3.0 | 4.2 | -5.9 | -4.5 | -5.0 | -2.1 | -3.9 | -2.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 |
Vacuole | -3.0 | 0.1 | -4.9 | -4.3 | -5.3 | – | -5.4 | -5.0 | -5.5 | 0.6 | 1.8 | 3.8 | 4.4 | 1.7 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0204 | 0.0004 | 0.0259 | 0.0415 | 0.0014 | 0.0016 | 0.0143 | 0.0001 | 0.0026 | 0.0069 | 0.0003 | 0 | – | – | – | – | – | – |
Bud | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | – | – | – | – | – | – |
Bud Neck | 0.0006 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0 | 0 | – | – | – | – | – | – |
Bud Periphery | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0004 | 0 | 0 | – | – | – | – | – | – |
Bud Site | 0.0011 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0011 | 0 | 0.0003 | 0.0001 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | – | – | – | – | – | – |
Cytoplasm | 0.0098 | 0.0007 | 0.0006 | 0.0007 | 0.0001 | 0.001 | 0.0077 | 0 | 0.0002 | 0.0001 | 0 | 0 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0033 | 0.0003 | 0.009 | 0.0031 | 0.0078 | 0.0065 | 0.019 | 0 | 0.0014 | 0.0041 | 0 | 0.0001 | – | – | – | – | – | – |
Eisosomes | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0041 | 0.0004 | 0.0018 | 0.0018 | 0.0004 | 0.0003 | 0.0085 | 0.0003 | 0.0019 | 0.0013 | 0.0002 | 0.0001 | – | – | – | – | – | – |
Endosome | 0.0197 | 0.0004 | 0.0044 | 0.003 | 0.0027 | 0.001 | 0.0617 | 0.0001 | 0.003 | 0.0017 | 0.0002 | 0.0001 | – | – | – | – | – | – |
Golgi | 0.0056 | 0.0001 | 0.0033 | 0.0029 | 0.0027 | 0.0017 | 0.0137 | 0.0001 | 0.0013 | 0.0041 | 0.0001 | 0.0002 | – | – | – | – | – | – |
Lipid Particles | 0.0113 | 0.0007 | 0.0076 | 0.003 | 0.0216 | 0.0026 | 0.0163 | 0.0007 | 0.0178 | 0.0672 | 0.0011 | 0.004 | – | – | – | – | – | – |
Mitochondria | 0.0056 | 0.0016 | 0.0036 | 0.0435 | 0.0077 | 0.0105 | 0.007 | 0.0009 | 0.0098 | 0.0057 | 0.0017 | 0.0002 | – | – | – | – | – | – |
None | 0.0044 | 0.0002 | 0.0009 | 0.0009 | 0.0004 | 0.0011 | 0.0043 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0 | – | – | – | – | – | – |
Nuclear Periphery | 0.855 | 0.8514 | 0.8301 | 0.7987 | 0.805 | 0.91 | 0.7571 | 0.9472 | 0.8888 | 0.8641 | 0.9479 | 0.9778 | – | – | – | – | – | – |
Nucleolus | 0.0016 | 0.008 | 0.002 | 0.0006 | 0.0094 | 0.0032 | 0.0028 | 0.0002 | 0.0024 | 0.0007 | 0.0006 | 0.0002 | – | – | – | – | – | – |
Nucleus | 0.0155 | 0.0871 | 0.0654 | 0.0577 | 0.0651 | 0.0383 | 0.0219 | 0.0173 | 0.0195 | 0.0036 | 0.0137 | 0.0021 | – | – | – | – | – | – |
Peroxisomes | 0.0019 | 0 | 0.0083 | 0.0111 | 0.0288 | 0.0033 | 0.0064 | 0 | 0.0008 | 0.001 | 0 | 0 | – | – | – | – | – | – |
Punctate Nuclear | 0.0108 | 0.0063 | 0.003 | 0.0046 | 0.0027 | 0.0019 | 0.0194 | 0.0003 | 0.0011 | 0.0022 | 0.0003 | 0.0003 | – | – | – | – | – | – |
Vacuole | 0.0016 | 0.0023 | 0.0015 | 0.0006 | 0.0023 | 0.0008 | 0.0032 | 0.0002 | 0.0016 | 0.0004 | 0.0002 | 0.0001 | – | – | – | – | – | – |
Vacuole Periphery | 0.0265 | 0.0396 | 0.0311 | 0.0254 | 0.0412 | 0.0156 | 0.0336 | 0.0325 | 0.0463 | 0.0358 | 0.0336 | 0.0148 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.7428 | 41.1614 | 37.7629 | 44.8021 | 45.2267 | 36.2578 | 48.9329 | 46.22 | 41.366 | 53.4846 |
Translational Efficiency | 1.1538 | 0.9957 | 1.0037 | 0.782 | 0.8988 | 1.0419 | 0.8453 | 0.7394 | 0.8299 | 0.7955 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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