Standard name | |
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Description | Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 2 | 5 |
Bud | 1 | 0 | 2 | 1 | 0 | 8 | 12 | 6 | 8 | 9 | 14 | 14 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 4 | 0 | 11 | 15 |
Bud Neck | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 4 | 7 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 204 | 157 | 204 | 170 | 263 | 271 | 364 | 278 | 200 | 223 | 150 | 236 | 195 | 384 | 413 | 113 | 68 | 59 | 187 | 154 | 108 | 186 | 338 | 342 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 7 | 5 | 3 | 30 | 0 | 3 | 0 | 0 | 0 |
Endosome | 2 | 1 | 3 | 3 | 6 | 2 | 5 | 13 | 3 | 0 | 2 | 2 | 1 | 0 | 3 | 11 | 8 | 1 | 7 | 0 | 5 | 2 | 1 | 6 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 2 | 0 | 1 | 0 |
Mitochondria | 13 | 5 | 13 | 32 | 46 | 156 | 295 | 250 | 287 | 320 | 318 | 319 | 0 | 2 | 0 | 4 | 2 | 1 | 59 | 6 | 59 | 6 | 14 | 17 |
Nucleus | 7 | 3 | 5 | 1 | 5 | 13 | 35 | 49 | 52 | 37 | 49 | 51 | 6 | 5 | 4 | 29 | 13 | 12 | 10 | 7 | 9 | 6 | 6 | 3 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 2 | 2 | 2 | 2 | 2 | 3 | 6 | 4 | 5 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 10 | 2 | 3 | 4 | 12 | 20 |
Vac/Vac Membrane | 4 | 6 | 38 | 9 | 23 | 26 | 32 | 36 | 22 | 29 | 38 | 22 | 9 | 21 | 24 | 84 | 61 | 37 | 32 | 13 | 29 | 7 | 15 | 16 |
Unique Cell Count | 232 | 169 | 230 | 195 | 312 | 383 | 587 | 512 | 459 | 488 | 445 | 505 | 211 | 401 | 430 | 210 | 130 | 100 | 373 | 192 | 245 | 225 | 428 | 447 |
Labelled Cell Count | 239 | 173 | 270 | 218 | 348 | 481 | 752 | 642 | 588 | 633 | 590 | 658 | 217 | 414 | 448 | 248 | 160 | 114 | 373 | 192 | 245 | 225 | 428 | 447 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 3.5 | 4.0 | 4.3 | 4.0 | 3.8 | 3.4 | 3.2 | 3.3 | 3.2 | 2.9 | 2.8 | 2.7 | 5.2 | 5.3 | 5.2 | 5.9 | 6.0 | 5.9 | 5.5 | 5.6 | 5.6 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.7 | 2.2 | 1.7 | 1.6 | 1.4 | 1.3 | 1.6 | 1.1 | 1.1 | 0.8 | 1.0 | 1.1 | 1.2 | 1.0 | 1.3 | 1.1 | 1.7 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.18 | -0.37 | -0.45 | -0.39 | -0.44 | -0.56 | -0.62 | -0.66 | 0.27 | 0.29 | 0.27 | 0.43 | 0.47 | 0.45 | 0.35 | 0.36 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.5 | 6.2 | 4.1 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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