Standard name
Description Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.93 0.89 0.87 0.84 0.71 0.62 0.54 0.44 0.46 0.34 0.47 0.92 0.96 0.96 0.54 0.52 0.59 0.5 0.8 0.44 0.83 0.79 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0.06 0.16 0.15 0.41 0.5 0.49 0.63 0.66 0.71 0.63 0 0 0 0 0 0 0.16 0 0.24 0 0 0
Nucleus 0 0 0 0 0 0 0.06 0.1 0.11 0.08 0.11 0.1 0 0 0 0.14 0.1 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0.17 0.05 0.07 0.07 0.05 0.07 0.05 0.06 0.09 0 0 0.05 0.06 0.4 0.47 0.37 0.09 0.07 0.12 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 4 0 2 5
Bud 1 0 2 1 0 8 12 6 8 9 14 14 0 0 0 0 1 0 5 0 4 0 11 15
Bud Neck 2 0 0 0 0 0 1 0 0 2 0 1 4 2 0 0 0 1 1 0 1 1 2 2
Bud Site 0 0 0 0 0 1 1 3 3 4 7 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 0 0 0 0 0 0 0 1 2 1 0 2 0 0 0 1 0 0 0 1 2
Cytoplasm 204 157 204 170 263 271 364 278 200 223 150 236 195 384 413 113 68 59 187 154 108 186 338 342
Endoplasmic Reticulum 1 0 0 0 0 0 1 1 1 1 0 0 0 0 2 7 5 3 30 0 3 0 0 0
Endosome 2 1 3 3 6 2 5 13 3 0 2 2 1 0 3 11 8 1 7 0 5 2 1 6
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 4 0 2 0 1 0
Mitochondria 13 5 13 32 46 156 295 250 287 320 318 319 0 2 0 4 2 1 59 6 59 6 14 17
Nucleus 7 3 5 1 5 13 35 49 52 37 49 51 6 5 4 29 13 12 10 7 9 6 6 3
Nuclear Periphery 1 0 0 0 0 0 1 0 2 2 4 2 0 0 0 0 0 0 0 0 1 0 1 1
Nucleolus 0 0 0 0 1 1 3 2 3 1 2 0 0 0 0 0 0 0 2 0 1 0 0 0
Peroxisomes 0 0 0 0 1 1 0 1 1 1 0 0 0 0 0 0 0 0 1 1 1 0 0 0
SpindlePole 3 0 2 2 2 2 2 3 6 4 5 6 1 0 0 0 0 0 10 2 3 4 12 20
Vac/Vac Membrane 4 6 38 9 23 26 32 36 22 29 38 22 9 21 24 84 61 37 32 13 29 7 15 16
Unique Cell Count 232 169 230 195 312 383 587 512 459 488 445 505 211 401 430 210 130 100 373 192 245 225 428 447
Labelled Cell Count 239 173 270 218 348 481 752 642 588 633 590 658 217 414 448 248 160 114 373 192 245 225 428 447


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.0 4.3 4.0 3.8 3.4 3.2 3.3 3.2 2.9 2.8 2.7 5.2 5.3 5.2 5.9 6.0 5.9 5.5 5.6 5.6
Std Deviation (1e-4) 0.8 0.7 1.7 2.2 1.7 1.6 1.4 1.3 1.6 1.1 1.1 0.8 1.0 1.1 1.2 1.0 1.3 1.1 1.7 1.6 1.6
Intensity Change (Log2) – – – -0.11 -0.18 -0.37 -0.45 -0.39 -0.44 -0.56 -0.62 -0.66 0.27 0.29 0.27 0.43 0.47 0.45 0.35 0.36 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.5 6.2 4.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lif1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available