Standard name | |
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Human Ortholog | |
Description | Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 2 | 5 |
Bud | 1 | 0 | 2 | 1 | 0 | 8 | 12 | 6 | 8 | 9 | 14 | 14 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 4 | 0 | 11 | 15 |
Bud Neck | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 4 | 7 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 204 | 157 | 204 | 170 | 263 | 271 | 364 | 278 | 200 | 223 | 150 | 236 | 195 | 384 | 413 | 113 | 68 | 59 | 187 | 154 | 108 | 186 | 338 | 342 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 7 | 5 | 3 | 30 | 0 | 3 | 0 | 0 | 0 |
Endosome | 2 | 1 | 3 | 3 | 6 | 2 | 5 | 13 | 3 | 0 | 2 | 2 | 1 | 0 | 3 | 11 | 8 | 1 | 7 | 0 | 5 | 2 | 1 | 6 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 2 | 0 | 1 | 0 |
Mitochondria | 13 | 5 | 13 | 32 | 46 | 156 | 295 | 250 | 287 | 320 | 318 | 319 | 0 | 2 | 0 | 4 | 2 | 1 | 59 | 6 | 59 | 6 | 14 | 17 |
Nucleus | 7 | 3 | 5 | 1 | 5 | 13 | 35 | 49 | 52 | 37 | 49 | 51 | 6 | 5 | 4 | 29 | 13 | 12 | 10 | 7 | 9 | 6 | 6 | 3 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 2 | 2 | 2 | 2 | 2 | 3 | 6 | 4 | 5 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 10 | 2 | 3 | 4 | 12 | 20 |
Vac/Vac Membrane | 4 | 6 | 38 | 9 | 23 | 26 | 32 | 36 | 22 | 29 | 38 | 22 | 9 | 21 | 24 | 84 | 61 | 37 | 32 | 13 | 29 | 7 | 15 | 16 |
Unique Cell Count | 232 | 169 | 230 | 195 | 312 | 383 | 587 | 512 | 459 | 488 | 445 | 505 | 211 | 401 | 430 | 210 | 130 | 100 | 373 | 192 | 245 | 225 | 428 | 447 |
Labelled Cell Count | 239 | 173 | 270 | 218 | 348 | 481 | 752 | 642 | 588 | 633 | 590 | 658 | 217 | 414 | 448 | 248 | 160 | 114 | 373 | 192 | 245 | 225 | 428 | 447 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.0 | 4.3 | 4.0 | 3.8 | 3.4 | 3.2 | 3.3 | 3.2 | 2.9 | 2.8 | 2.7 | 5.2 | 5.3 | 5.2 | 5.9 | 6.0 | 5.9 | 5.5 | 5.6 | 5.6 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.7 | 2.2 | 1.7 | 1.6 | 1.4 | 1.3 | 1.6 | 1.1 | 1.1 | 0.8 | 1.0 | 1.1 | 1.2 | 1.0 | 1.3 | 1.1 | 1.7 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.18 | -0.37 | -0.45 | -0.39 | -0.44 | -0.56 | -0.62 | -0.66 | 0.27 | 0.29 | 0.27 | 0.43 | 0.47 | 0.45 | 0.35 | 0.36 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.5 | 6.2 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0367 | 0.517 | 0.6607 | 0.0848 | 0.0728 | 0.5126 | -0.2859 | -0.8336 | -0.7157 | -0.721 | -0.1637 | -0.6943 | -0.6395 | -1.0767 | -1.2578 | -1.2774 | -1.0887 | -1.3555 |
Actin | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0005 | 0.0881 | 0.0009 | 0.0166 | 0.0442 | 0.0914 | 0.0112 | 0.0391 | 0.0009 | 0.0155 | 0.0015 | 0.0193 | 0.0004 |
Bud | 0.0003 | 0.0126 | 0.0025 | 0.0001 | 0.0078 | 0.0003 | 0.0006 | 0.0014 | 0.0011 | 0.0011 | 0.0021 | 0.0013 | 0.0021 | 0.0006 | 0.0025 | 0.0001 | 0.0004 | 0.0019 |
Bud Neck | 0.0001 | 0.0001 | 0.0003 | 0.0007 | 0.0001 | 0.0005 | 0.0052 | 0.0005 | 0.0052 | 0.0009 | 0.0024 | 0.0013 | 0.0011 | 0.0002 | 0.0006 | 0.0008 | 0.0005 | 0.0011 |
Bud Periphery | 0.0007 | 0.0019 | 0.0033 | 0.0001 | 0.0086 | 0.0005 | 0.0009 | 0.0006 | 0.0009 | 0.0014 | 0.0061 | 0.0017 | 0.0025 | 0.0006 | 0.0009 | 0.0001 | 0.0006 | 0.0006 |
Bud Site | 0.0002 | 0.0067 | 0.0011 | 0.0007 | 0.0001 | 0 | 0.0038 | 0.0099 | 0.0099 | 0.0009 | 0.0067 | 0.0004 | 0.0021 | 0.0015 | 0.0032 | 0.0008 | 0.0013 | 0.0002 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.1 | 0.1593 | 0.1024 | 0.1879 | 0.1583 | 0.218 | 0.1221 | 0.2251 | 0.1757 | 0.1531 | 0.1587 | 0.355 | 0.1858 | 0.3016 | 0.2316 | 0.2428 | 0.1697 | 0.3119 |
Cytoplasmic Foci | 0.0041 | 0.0025 | 0.0027 | 0.0029 | 0.0057 | 0.0095 | 0.0262 | 0.0044 | 0.0167 | 0.0094 | 0.031 | 0.0193 | 0.0374 | 0.0066 | 0.0119 | 0.013 | 0.0055 | 0.0273 |
Eisosomes | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.002 | 0.0027 | 0.0016 | 0.0012 | 0.0007 | 0.0013 | 0.0112 | 0.0023 | 0.0038 | 0.0022 | 0.0025 | 0.0054 | 0.0061 | 0.0022 | 0.0034 | 0.0016 | 0.0042 | 0.0017 |
Endosome | 0.0011 | 0.0005 | 0.0009 | 0.0013 | 0.0003 | 0.0012 | 0.0427 | 0.002 | 0.0081 | 0.0081 | 0.0189 | 0.0166 | 0.0264 | 0.0019 | 0.0071 | 0.0064 | 0.0233 | 0.0044 |
Golgi | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0073 | 0.0001 | 0.0034 | 0.0036 | 0.0113 | 0.0055 | 0.0055 | 0.0001 | 0.0015 | 0.001 | 0.005 | 0.0002 |
Lipid Particles | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0072 | 0.0007 | 0.0055 | 0.0047 | 0.0273 | 0.0046 | 0.0122 | 0.0002 | 0.0012 | 0.0035 | 0.017 | 0.0008 |
Mitochondria | 0.0022 | 0.0004 | 0.002 | 0.0003 | 0.0003 | 0.0006 | 0.0037 | 0.0003 | 0.0075 | 0.0017 | 0.0098 | 0.0052 | 0.0089 | 0.0007 | 0.0019 | 0.0008 | 0.0051 | 0.0012 |
None | 0.8818 | 0.7854 | 0.8228 | 0.7436 | 0.8131 | 0.7371 | 0.5611 | 0.7238 | 0.7097 | 0.7071 | 0.5603 | 0.5008 | 0.577 | 0.6293 | 0.6429 | 0.6741 | 0.6021 | 0.5758 |
Nuclear Periphery | 0.001 | 0.0017 | 0.005 | 0.0015 | 0.0007 | 0.0029 | 0.0326 | 0.0051 | 0.0029 | 0.0082 | 0.0039 | 0.0085 | 0.0188 | 0.0032 | 0.0087 | 0.0028 | 0.0191 | 0.0029 |
Nucleolus | 0.0003 | 0.0004 | 0.0007 | 0.0005 | 0.0001 | 0.0003 | 0.0025 | 0.001 | 0.0005 | 0.0023 | 0.0027 | 0.0006 | 0.0086 | 0.0005 | 0.0016 | 0.001 | 0.001 | 0.0017 |
Nucleus | 0.003 | 0.0231 | 0.0484 | 0.0548 | 0.0013 | 0.0201 | 0.0455 | 0.0147 | 0.0194 | 0.0202 | 0.0026 | 0.038 | 0.0332 | 0.0423 | 0.0471 | 0.0366 | 0.1139 | 0.0488 |
Peroxisomes | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0115 | 0.0002 | 0.0062 | 0.0042 | 0.0506 | 0.005 | 0.0057 | 0.0002 | 0.0005 | 0.0025 | 0.0016 | 0.0003 |
Punctate Nuclear | 0.0013 | 0.0012 | 0.0042 | 0.0023 | 0.0015 | 0.0059 | 0.016 | 0.0051 | 0.004 | 0.0249 | 0.0043 | 0.0168 | 0.0175 | 0.0059 | 0.0154 | 0.0092 | 0.0067 | 0.0175 |
Vacuole | 0.0006 | 0.0006 | 0.0008 | 0.0014 | 0.0004 | 0.0004 | 0.0081 | 0.0011 | 0.0015 | 0.0009 | 0.0054 | 0.0014 | 0.0058 | 0.0011 | 0.0015 | 0.001 | 0.0019 | 0.001 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0028 | 0.0003 | 0.0004 | 0.0004 | 0.0013 | 0.0011 | 0.0029 | 0.0002 | 0.0006 | 0.0002 | 0.0017 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.4863 | 4.6748 | 5.2033 | 6.5383 | 2.2601 | 5.2265 | 6.4991 | 6.6462 | 10.269 | 8.7577 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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