Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.05 0.08 0.11 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.97 0.95 0.91 0.88 0.8 0.81 0.8 0.79 0.76 0.98 0.99 0.99 0.94 0.96 0.93 0.97 0.96 0.95 0.94 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.1 0.08 0.11 0.2 0.15 0.2 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 2 0 1 0 2 0 0 0 1 0 0 2 1 0 0 1 2
Bud 0 0 11 1 0 2 7 7 1 5 2 4 1 0 3 0 0 0 1 1 1 2 5 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 16 14 3 5 7 11 9 11 12 13 19 24 31 28 36 25 27 19 0 0 0 1 0 0
Cytoplasm 1010 586 519 311 438 317 566 455 328 425 316 401 685 654 761 312 234 229 997 566 536 437 553 515
Endoplasmic Reticulum 4 1 4 1 4 4 22 15 7 18 12 19 8 0 1 13 7 6 1 1 2 4 9 8
Endosome 1 0 1 0 1 1 0 0 0 1 0 0 1 0 2 0 1 1 7 3 3 3 3 2
Golgi 2 3 1 0 0 0 0 0 0 0 0 0 1 1 0 1 0 1 2 2 1 3 3 3
Mitochondria 8 2 10 17 14 36 53 60 81 80 78 104 0 0 2 3 1 3 3 2 5 1 1 0
Nucleus 8 2 4 1 2 4 10 8 4 7 8 9 2 4 0 2 2 2 0 3 0 1 1 2
Nuclear Periphery 0 0 0 0 0 1 3 0 0 0 0 2 0 0 0 0 0 0 0 1 0 2 1 0
Nucleolus 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 9 2 0 2 6 2 9 44 13 23 9 20 2 2 7 12 5 9 3 4 7 3 3 3
Unique Cell Count 1035 594 530 320 459 350 644 571 406 532 399 531 696 659 771 333 245 245 1030 593 566 465 594 549
Labelled Cell Count 1058 610 553 339 472 379 681 602 446 573 445 585 731 689 812 370 277 271 1030 593 566 465 594 549


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 12.7 6.1 7.7 8.7 7.9 8.3 8.4 7.8 8.2 8.2 8.2 12.2 12.5 12.7 21.2 20.2 20.3 12.1 11.9 11.7
Std Deviation (1e-4) 1.6 2.2 1.1 1.5 1.6 1.5 1.6 1.6 1.5 1.6 1.7 1.6 2.0 2.2 2.4 6.1 5.3 5.1 2.6 2.6 2.0
Intensity Change (Log2) – – – 0.32 0.51 0.38 0.43 0.46 0.34 0.42 0.42 0.42 0.99 1.03 1.05 1.79 1.72 1.73 0.99 0.96 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -3.1 -1.8 -1.4 -1.1 -2.5 -1.5 -2.0 -1.8 -3.4 0 -2.9 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3.0 1.4 2.0 2.9 2.5 4.2 4.1 4.1 4.0 4.3 5.6 7.0 5.6
Cytoplasm -0.7 -2.2 -4.9 -6.5 -9.5 -8.9 -9.4 -9.4 -10.8 0.6 2.0 1.1 -3.2 -1.9 -3.1
Endoplasmic Reticulum 0 0 0 3.1 2.4 0 3.0 2.6 3.2 0.7 0 0 3.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 1.2 5.5 4.8 5.9 9.2 7.7 9.1 9.3 0 0 0 -1.2 0 0
Nucleus 0 0 0 1.3 1.0 0 0.9 1.7 1.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 6.5 4.1 4.8 0 4.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4991 4.7496 4.0448 3.7467 3.9674 4.043 2.8727 5.2744 4.4746 4.0793 4.3639 5.5359 3.6936 5.9698 4.4976 4.6238 3.2853 4.6153
Actin 0.0441 0.0007 0.0057 0.0036 0.0004 0.0191 0.022 0.0002 0.01 0.0515 0.0003 0.0001 0.0327 0.0002 0.0023 0.0016 0.0023 0.001
Bud 0.0006 0.0005 0.0003 0.0003 0 0.001 0.0003 0.0001 0.0005 0.0001 0.0001 0 0.0005 0 0.0006 0.0001 0.0002 0.0004
Bud Neck 0.0018 0.0002 0.0004 0.0009 0.0004 0.0009 0.0013 0.0001 0.0007 0.0003 0.0004 0.0005 0.0022 0 0.0053 0.0002 0.0003 0.0006
Bud Periphery 0.0008 0.0002 0.0002 0.0001 0 0.0007 0.0003 0 0.0004 0 0 0 0.0006 0 0.0009 0 0.0002 0.0004
Bud Site 0.0038 0.0018 0.0011 0.001 0 0.0004 0.0016 0.0004 0.0016 0.0002 0 0 0.0046 0.0001 0.0062 0.0002 0.0002 0.0022
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0004 0 0.0004 0.0001 0.0001 0.0001
Cytoplasm 0.7053 0.9672 0.8798 0.8722 0.9874 0.8698 0.6482 0.9457 0.9067 0.9328 0.9965 0.989 0.8611 0.9819 0.9231 0.9542 0.8435 0.9191
Cytoplasmic Foci 0.0427 0.0027 0.0209 0.0084 0.0023 0.0095 0.072 0.0013 0.0213 0.0013 0.0005 0.0019 0.0233 0.0021 0.0139 0.0034 0.0056 0.0134
Eisosomes 0.0003 0 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0 0 0.0011 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0107 0.0019 0.004 0.0034 0.0021 0.0028 0.0045 0.0009 0.0019 0.0013 0.0003 0.0009 0.0029 0.0008 0.0122 0.0109 0.0026 0.0014
Endosome 0.0412 0.0014 0.0293 0.0507 0.0006 0.0218 0.0506 0.0004 0.0132 0.0003 0.0001 0.0005 0.0088 0.0007 0.0068 0.0018 0.0114 0.0293
Golgi 0.0135 0.0005 0.0059 0.0064 0 0.0278 0.0197 0 0.007 0.0009 0 0 0.0084 0.0001 0.0017 0.0003 0.0168 0.0071
Lipid Particles 0.0141 0.0001 0.0028 0.0063 0 0.0088 0.0274 0 0.0072 0.0002 0 0 0.0119 0.0001 0.0109 0.0002 0.0029 0.0014
Mitochondria 0.0107 0.0068 0.0224 0.0025 0 0.0067 0.0213 0 0.0016 0.0002 0 0 0.0057 0 0.0017 0.0001 0.1029 0.0055
None 0.0379 0.0118 0.0183 0.0311 0.0049 0.0082 0.0964 0.0492 0.0105 0.0074 0.0011 0.0057 0.0117 0.0129 0.0042 0.0241 0.0017 0.0068
Nuclear Periphery 0.026 0.0008 0.0019 0.0043 0.0004 0.0024 0.0019 0.0003 0.0004 0.0002 0.0001 0.0002 0.0009 0.0002 0.0021 0.0009 0.0009 0.0007
Nucleolus 0.001 0 0.0001 0.0002 0 0.0004 0.0003 0 0.0002 0 0 0 0.0002 0 0.0009 0 0 0.0002
Nucleus 0.0144 0.002 0.0013 0.0028 0.0011 0.0017 0.0016 0.0008 0.0008 0.0007 0.0005 0.0008 0.001 0.0004 0.001 0.0009 0.0009 0.0009
Peroxisomes 0.0141 0.0004 0.0029 0.0009 0 0.0057 0.0185 0 0.0109 0.0021 0 0 0.0184 0 0.0015 0 0.0049 0.0029
Punctate Nuclear 0.0083 0.0003 0.0009 0.0016 0.0002 0.0111 0.0059 0.0002 0.0041 0.0001 0 0.0001 0.0015 0.0001 0.0009 0.0003 0.0001 0.0008
Vacuole 0.0049 0.0004 0.0011 0.0021 0.0001 0.0006 0.0042 0.0003 0.0006 0.0001 0.0001 0.0001 0.0016 0.0002 0.0023 0.0006 0.0019 0.0035
Vacuole Periphery 0.0034 0.0001 0.0005 0.0012 0 0.0006 0.0017 0 0.0002 0 0 0 0.0004 0 0.001 0.0001 0.0007 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.6415 33.3557 30.0552 46.9317 48.3782 34.6288 44.5664 59.3457 49.2253 45.9098
Translational Efficiency 2.1075 2.9388 3.0167 2.0893 1.6448 3.7205 2.5729 2.0122 2.5223 2.4605

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1680 2249 292 1021 1388 1681 1786 879 3068 3930 2078 1900

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 924.06 1068.58 1534.90 1808.61 894.76 1002.10 1292.45 1647.48 910.80 1040.14 1326.52 1734.07
Standard Deviation 139.03 172.17 199.50 426.74 157.82 140.82 246.06 363.15 148.55 162.87 254.42 406.60
Intensity Change Log 2 — 0.209636 0.732086 0.968823 — 0.163454 0.530536 0.880688 — 0.187102 0.636448 0.926138

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000164 0.000146 0.000722 0.004000 0.000184 0.000499 0.000294 0.000657 0.000173 0.000297 0.000354 0.002453
Bud Neck 0.002561 0.002651 0.002833 0.004987 0.001423 0.004667 0.000902 0.003495 0.002047 0.003514 0.001173 0.004296
Bud Site 0.008630 0.009405 0.016826 0.029500 0.007161 0.019562 0.011821 0.021777 0.007966 0.013750 0.012524 0.025927
Cell Periphery 0.000134 0.000056 0.000328 0.000322 0.000104 0.000090 0.000075 0.000190 0.000121 0.000070 0.000110 0.000261
Cytoplasm 0.780795* 0.828070* 0.655632* 0.692514* 0.803868* 0.730170* 0.893366* 0.735908* 0.791234* 0.786195* 0.859960* 0.712590*
Cytoplasmic Foci 0.093386 0.064279 0.046077 0.029579 0.082222 0.124561 0.015837 0.030519 0.088335 0.090064 0.020086 0.030013
Eisosomes 0.000033 0.000011 0.000022 0.000015 0.000020 0.000032 0.000005 0.000013 0.000027 0.000020 0.000007 0.000014
Endoplasmic Reticulum 0.000785 0.000225 0.000990 0.001201 0.000813 0.000488 0.000070 0.000398 0.000798 0.000337 0.000199 0.000830
Endosome 0.003941 0.002745 0.006521 0.016592 0.004638 0.007613 0.001164 0.016638 0.004256 0.004827 0.001917 0.016613
Golgi 0.001354 0.001269 0.000693 0.028368 0.000904 0.002793 0.000798 0.015934 0.001151 0.001921 0.000783 0.022615
Lipid Particles 0.000956 0.000452 0.002359 0.007025 0.000867 0.000723 0.000443 0.004460 0.000916 0.000568 0.000712 0.005838
Mitochondria 0.000520 0.001578 0.000060 0.013476 0.000171 0.001264 0.000074 0.002081 0.000362 0.001443 0.000072 0.008204
Mitotic Spindle 0.001188 0.002513 0.005858 0.035311 0.000664 0.005525 0.008283 0.049754 0.000951 0.003801 0.007942 0.041993
None 0.008158 0.004662 0.003230 0.003812 0.005603 0.001637 0.002697 0.004433 0.007002 0.003368 0.002772 0.004099
Nuclear Periphery 0.000053 0.000054 0.000363 0.001783 0.000146 0.000051 0.000060 0.000234 0.000095 0.000052 0.000103 0.001066
Nuclear Periphery Foci 0.002250 0.001109 0.013189 0.009091 0.002540 0.000910 0.001576 0.003248 0.002381 0.001024 0.003208 0.006388
Nucleolus 0.000307 0.000246 0.000105 0.000300 0.000368 0.000258 0.000062 0.000590 0.000335 0.000251 0.000068 0.000434
Nucleus 0.037240 0.032412 0.061340 0.040656 0.038513 0.017595 0.033082 0.043062 0.037816 0.026074 0.037053 0.041769
Peroxisomes 0.000589 0.001354 0.000267 0.003844 0.000616 0.002064 0.000243 0.001948 0.000601 0.001658 0.000246 0.002967
Vacuole 0.056458 0.046352 0.182320 0.067533 0.048685 0.078729 0.029019 0.060936 0.052942 0.060201 0.050561 0.064481
Vacuole Periphery 0.000495 0.000411 0.000265 0.010092 0.000489 0.000767 0.000130 0.003725 0.000492 0.000563 0.000149 0.007147

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.18 -10.00 -2.72 -2.76 -0.88 -1.59 -3.88 -3.21 0.57 -0.44 -1.46 -6.89 -3.14 -2.33 -1.88
Bud Neck -0.16 -4.74 -2.05 -1.82 2.53 -7.90 1.84 -4.59 5.18 -5.97 -5.35 2.51 -3.76 2.07 -6.55
Bud Site -0.58 -7.14 -4.66 -4.22 2.05 -8.54 -3.20 -5.34 3.68 -2.18 -6.78 -4.19 -6.80 -0.76 -2.47
Cell Periphery 10.90 -9.50 -6.76 -10.56 2.18 1.94 -0.10 -4.07 -5.55 -3.99 9.42 -2.48 -7.81 -11.96 -5.97
Cytoplasm -7.08 13.33 10.62 15.56 -7.15 9.42 -3.09 10.43 2.99 12.99 1.69 1.10 14.84 13.58 12.42
Cytoplasmic Foci 7.89 8.15 21.90 15.67 7.70 -8.74 18.71 17.32 25.32 -0.79 -0.75 26.66 27.87 28.79 2.19
Eisosomes 10.53 1.66 10.77 1.42 9.82 -6.23 10.05 8.45 14.18 -1.00 4.89 12.60 13.45 12.64 2.19
Endoplasmic Reticulum 3.06 -0.92 2.75 -0.54 6.46 2.02 5.86 5.65 3.18 -0.26 3.74 5.08 5.16 2.36 0.78
Endosome 3.36 -3.30 1.11 -0.53 3.24 -4.36 7.88 1.69 4.68 -2.70 -1.44 8.86 1.99 2.86 -2.80
Golgi 0.69 5.69 -2.20 -2.28 -2.71 -3.81 2.93 -1.61 0.28 -2.16 -3.06 5.23 -2.69 -1.87 -3.49
Lipid Particles 4.94 -9.13 -7.33 -8.19 -3.40 1.24 -1.72 -4.23 -4.53 -3.73 4.57 -4.20 -8.40 -9.30 -7.06
Mitochondria -1.46 2.09 -1.50 -0.54 -1.96 -2.37 2.25 -1.13 1.94 -2.06 -2.40 2.46 -1.61 0.09 -2.17
Mitotic Spindle -1.42 -2.44 -5.29 -4.62 -3.02 -3.27 -4.41 -4.44 -2.17 -2.19 -3.40 -4.58 -6.89 -4.84 -3.60
None 3.31 5.90 5.94 4.45 -0.24 3.78 3.57 2.84 -2.02 -1.60 4.83 7.00 6.36 2.38 -1.35
Nuclear Periphery -1.06 -14.71 -4.31 -4.25 0.07 2.26 0.37 -2.09 -10.56 -6.29 2.07 -4.57 -4.78 -5.68 -3.42
Nuclear Periphery Foci 5.35 -11.68 -4.62 -6.76 8.96 8.36 1.30 -0.73 -7.76 -1.88 9.24 -6.05 -4.42 -9.19 0.90
Nucleolus 1.41 8.23 5.32 3.32 -2.23 1.25 3.93 2.11 1.10 -2.01 1.75 7.05 3.90 2.56 -3.24
Nucleus 2.70 -4.59 -4.48 -6.48 2.12 10.94 -3.14 0.41 -10.20 3.56 9.43 -5.67 -3.00 -10.83 2.27
Peroxisomes -3.05 4.99 -0.94 1.38 -1.98 -6.29 3.46 0.47 6.17 -2.18 -6.04 5.96 -0.55 4.16 -3.03
Vacuole 2.16 -13.56 -18.59 -19.70 5.20 -8.68 -8.92 -18.36 -14.19 -13.76 -4.45 -14.15 -26.11 -24.16 -12.83
Vacuole Periphery 0.96 2.81 -2.70 -2.98 -3.38 -2.00 4.76 -0.63 0.55 -2.65 -0.73 5.97 -2.72 -2.53 -4.36
Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mms2

Mms2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mms2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available