Standard name
Description Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.05 0.08 0.11 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.97 0.95 0.91 0.88 0.8 0.81 0.8 0.79 0.76 0.98 0.99 0.99 0.94 0.96 0.93 0.97 0.96 0.95 0.94 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.1 0.08 0.11 0.2 0.15 0.2 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 2 0 1 0 2 0 0 0 1 0 0 2 1 0 0 1 2
Bud 0 0 11 1 0 2 7 7 1 5 2 4 1 0 3 0 0 0 1 1 1 2 5 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 16 14 3 5 7 11 9 11 12 13 19 24 31 28 36 25 27 19 0 0 0 1 0 0
Cytoplasm 1010 586 519 311 438 317 566 455 328 425 316 401 685 654 761 312 234 229 997 566 536 437 553 515
Endoplasmic Reticulum 4 1 4 1 4 4 22 15 7 18 12 19 8 0 1 13 7 6 1 1 2 4 9 8
Endosome 1 0 1 0 1 1 0 0 0 1 0 0 1 0 2 0 1 1 7 3 3 3 3 2
Golgi 2 3 1 0 0 0 0 0 0 0 0 0 1 1 0 1 0 1 2 2 1 3 3 3
Mitochondria 8 2 10 17 14 36 53 60 81 80 78 104 0 0 2 3 1 3 3 2 5 1 1 0
Nucleus 8 2 4 1 2 4 10 8 4 7 8 9 2 4 0 2 2 2 0 3 0 1 1 2
Nuclear Periphery 0 0 0 0 0 1 3 0 0 0 0 2 0 0 0 0 0 0 0 1 0 2 1 0
Nucleolus 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 9 2 0 2 6 2 9 44 13 23 9 20 2 2 7 12 5 9 3 4 7 3 3 3
Unique Cell Count 1035 594 530 320 459 350 644 571 406 532 399 531 696 659 771 333 245 245 1030 593 566 465 594 549
Labelled Cell Count 1058 610 553 339 472 379 681 602 446 573 445 585 731 689 812 370 277 271 1030 593 566 465 594 549


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 12.7 6.1 7.7 8.7 7.9 8.3 8.4 7.8 8.2 8.2 8.2 12.2 12.5 12.7 21.2 20.2 20.3 12.1 11.9 11.7
Std Deviation (1e-4) 1.6 2.2 1.1 1.5 1.6 1.5 1.6 1.6 1.5 1.6 1.7 1.6 2.0 2.2 2.4 6.1 5.3 5.1 2.6 2.6 2.0
Intensity Change (Log2) – – – 0.32 0.51 0.38 0.43 0.46 0.34 0.42 0.42 0.42 0.99 1.03 1.05 1.79 1.72 1.73 0.99 0.96 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -3.1 -1.8 -1.4 -1.1 -2.5 -1.5 -2.0 -1.8 -3.4 0 -2.9 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3.0 1.4 2.0 2.9 2.5 4.2 4.1 4.1 4.0 4.3 5.6 7.0 5.6
Cytoplasm -0.7 -2.2 -4.9 -6.5 -9.5 -8.9 -9.4 -9.4 -10.8 0.6 2.0 1.1 -3.2 -1.9 -3.1
Endoplasmic Reticulum 0 0 0 3.1 2.4 0 3.0 2.6 3.2 0.7 0 0 3.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 1.2 5.5 4.8 5.9 9.2 7.7 9.1 9.3 0 0 0 -1.2 0 0
Nucleus 0 0 0 1.3 1.0 0 0.9 1.7 1.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 6.5 4.1 4.8 0 4.5 0 0 0 0 0 0