Standard name | |
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Description | Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 2 |
Bud | 0 | 0 | 11 | 1 | 0 | 2 | 7 | 7 | 1 | 5 | 2 | 4 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 5 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 16 | 14 | 3 | 5 | 7 | 11 | 9 | 11 | 12 | 13 | 19 | 24 | 31 | 28 | 36 | 25 | 27 | 19 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 1010 | 586 | 519 | 311 | 438 | 317 | 566 | 455 | 328 | 425 | 316 | 401 | 685 | 654 | 761 | 312 | 234 | 229 | 997 | 566 | 536 | 437 | 553 | 515 |
Endoplasmic Reticulum | 4 | 1 | 4 | 1 | 4 | 4 | 22 | 15 | 7 | 18 | 12 | 19 | 8 | 0 | 1 | 13 | 7 | 6 | 1 | 1 | 2 | 4 | 9 | 8 |
Endosome | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 7 | 3 | 3 | 3 | 3 | 2 |
Golgi | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 2 | 1 | 3 | 3 | 3 |
Mitochondria | 8 | 2 | 10 | 17 | 14 | 36 | 53 | 60 | 81 | 80 | 78 | 104 | 0 | 0 | 2 | 3 | 1 | 3 | 3 | 2 | 5 | 1 | 1 | 0 |
Nucleus | 8 | 2 | 4 | 1 | 2 | 4 | 10 | 8 | 4 | 7 | 8 | 9 | 2 | 4 | 0 | 2 | 2 | 2 | 0 | 3 | 0 | 1 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 9 | 2 | 0 | 2 | 6 | 2 | 9 | 44 | 13 | 23 | 9 | 20 | 2 | 2 | 7 | 12 | 5 | 9 | 3 | 4 | 7 | 3 | 3 | 3 |
Unique Cell Count | 1035 | 594 | 530 | 320 | 459 | 350 | 644 | 571 | 406 | 532 | 399 | 531 | 696 | 659 | 771 | 333 | 245 | 245 | 1030 | 593 | 566 | 465 | 594 | 549 |
Labelled Cell Count | 1058 | 610 | 553 | 339 | 472 | 379 | 681 | 602 | 446 | 573 | 445 | 585 | 731 | 689 | 812 | 370 | 277 | 271 | 1030 | 593 | 566 | 465 | 594 | 549 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 12.7 | 6.1 | 7.7 | 8.7 | 7.9 | 8.3 | 8.4 | 7.8 | 8.2 | 8.2 | 8.2 | 12.2 | 12.5 | 12.7 | 21.2 | 20.2 | 20.3 | 12.1 | 11.9 | 11.7 |
Std Deviation (1e-4) | 1.6 | 2.2 | 1.1 | 1.5 | 1.6 | 1.5 | 1.6 | 1.6 | 1.5 | 1.6 | 1.7 | 1.6 | 2.0 | 2.2 | 2.4 | 6.1 | 5.3 | 5.1 | 2.6 | 2.6 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.32 | 0.51 | 0.38 | 0.43 | 0.46 | 0.34 | 0.42 | 0.42 | 0.42 | 0.99 | 1.03 | 1.05 | 1.79 | 1.72 | 1.73 | 0.99 | 0.96 | 0.93 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -3.1 | -1.8 | -1.4 | -1.1 | -2.5 | -1.5 | -2.0 | -1.8 | -3.4 | 0 | -2.9 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 3.0 | 1.4 | 2.0 | 2.9 | 2.5 | 4.2 | 4.1 | 4.1 | 4.0 | 4.3 | 5.6 | 7.0 | 5.6 |
Cytoplasm | -0.7 | -2.2 | -4.9 | -6.5 | -9.5 | -8.9 | -9.4 | -9.4 | -10.8 | 0.6 | 2.0 | 1.1 | -3.2 | -1.9 | -3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.1 | 2.4 | 0 | 3.0 | 2.6 | 3.2 | 0.7 | 0 | 0 | 3.2 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.8 | 1.2 | 5.5 | 4.8 | 5.9 | 9.2 | 7.7 | 9.1 | 9.3 | 0 | 0 | 0 | -1.2 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 1.3 | 1.0 | 0 | 0.9 | 1.7 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 6.5 | 4.1 | 4.8 | 0 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |