Standard name
Human Ortholog
Description Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.06 0 0.05 0.05 0 0 0 0 0.05 0.09 0.14 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.16 0.07 0.14 0 0.12 0.09 0.07 0.06 0 0 0 0.05 0.11 0.19 0.2 0.36 0.29 0.22 0.18 0.16 0.2 0.16 0.19 0.25
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.59 0.65 0.32 0.52 0.37 0.57 0.6 0.48 0.79 0.79 0.79 0.6 0.54 0.29 0.35 0.11 0.15 0.12 0 0.05 0.07 0.08 0.06 0.05
Nucleus 0.05 0 0.08 0.13 0.07 0.06 0.13 0.15 0.09 0.08 0.12 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.46 0.43 0.32 0.37 0.44 0.36 0.27 0.21 0.23 0.23 0.23 0.3 0.44 0.55 0.36 0.46 0.43 0.49 0.57 0.57 0.54 0.55 0.5 0.46
Nucleolus 0.22 0.12 0 0.06 0.06 0.09 0.06 0.08 0.13 0.15 0.16 0.14 0.25 0.16 0.14 0.15 0.21 0.15 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
SpindlePole 0 0 0.13 0.05 0.06 0.05 0.06 0.12 0.07 0.05 0 0.1 0 0 0 0.05 0.06 0 0.09 0.07 0 0.05 0.08 0.08
Vac/Vac Membrane 0.14 0.15 0.18 0.06 0.08 0.05 0.06 0.08 0 0 0 0.05 0.07 0.11 0.09 0.26 0.32 0.33 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 3 1 1 0 2 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0 3 5
Bud 0 1 2 0 1 5 5 7 5 7 9 10 4 0 2 0 1 1 2 1 1 2 3 4
Bud Neck 0 0 5 0 2 0 1 2 0 0 0 2 0 0 0 0 0 0 0 0 2 0 1 2
Bud Site 0 1 1 1 1 2 5 5 7 5 10 8 1 0 4 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 2 10 12 14 15 18 19 10 6 8 9 16 32 46 5 3 5 0 0 1 0 0 0
Endoplasmic Reticulum 1 4 3 3 2 3 0 0 0 0 1 0 2 5 2 4 3 0 0 0 2 0 2 2
Endosome 24 16 30 8 27 32 27 23 1 3 4 19 33 69 64 42 21 18 29 41 66 22 72 86
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 2 0 3 2
Mitochondria 87 151 67 99 80 201 231 172 236 294 319 236 164 105 116 13 11 10 7 12 22 11 22 18
Nucleus 7 10 17 24 15 22 50 53 28 29 48 43 17 5 7 4 1 2 3 3 2 1 2 0
Nuclear Periphery 68 101 66 71 97 128 104 75 70 84 92 116 132 200 119 53 31 40 92 145 180 75 191 160
Nucleolus 33 28 5 12 13 31 24 30 38 56 66 54 75 58 46 17 15 12 2 5 13 5 12 2
Peroxisomes 0 2 1 1 3 5 7 2 2 3 0 2 2 7 5 0 0 2 2 5 5 1 20 19
SpindlePole 2 8 26 10 14 18 23 44 21 18 18 39 9 9 14 6 4 2 14 18 14 7 30 27
Vac/Vac Membrane 21 34 38 12 18 19 22 27 7 14 7 18 20 41 31 30 23 27 4 12 8 4 7 7
Unique Cell Count 147 233 207 191 218 352 385 358 298 372 404 391 303 363 327 116 72 81 163 253 335 138 379 349
Labelled Cell Count 249 359 275 254 289 482 519 460 425 519 582 557 475 532 457 176 114 120 163 253 335 138 379 349


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.7 5.6 5.1 5.7 4.6 4.8 5.1 4.1 4.0 3.9 4.6 5.3 5.6 5.6 8.5 8.4 8.2 5.2 5.2 5.5
Std Deviation (1e-4) 0.8 0.8 1.1 1.1 1.4 1.0 1.3 1.0 1.0 0.9 0.8 1.0 1.0 1.3 1.5 1.8 1.9 1.6 1.2 1.1 1.4
Intensity Change (Log2) – – – -0.14 0.04 -0.27 -0.21 -0.12 -0.43 -0.46 -0.51 -0.27 -0.07 0.0 0.0 0.61 0.6 0.56 -0.09 -0.09 -0.02

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.6 2.4 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1114 1.402 1.1617 0.8538 1.3225 0.9803 1.6508 1.8126 1.9806 1.5488 1.9427 1.4406 1.8027 1.444 1.3574 1.5185 0.803 1.2461
Actin 0.0321 0.0052 0.0063 0.0018 0.0335 0.0136 0.0353 0.0005 0.0109 0.0049 0.0013 0.0056 0.0551 0.002 0.0253 0.0018 0.001 0.0042
Bud 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0.0004 0 0.0004 0.0001 0.0001 0.0001 0.0007 0.0001 0.0004 0.0002 0 0.0001
Bud Neck 0.0137 0.0003 0.0002 0.0011 0.0007 0.0011 0.0004 0.0001 0.0005 0.0005 0.0005 0.0003 0.0004 0.0001 0.0024 0.0002 0.0001 0.001
Bud Periphery 0.0006 0.0004 0.0002 0.0003 0.0003 0.0005 0.0009 0.0002 0.0008 0.0005 0.0003 0.0003 0.0012 0.0003 0.0014 0.0003 0.0002 0.0003
Bud Site 0.0074 0.0008 0.0004 0.0015 0.0004 0.0004 0.0011 0.0001 0.0058 0.0006 0.0003 0.0004 0.0023 0.0004 0.0063 0.0009 0.0001 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0.0003 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0009 0.0001 0.0001 0.0001
Cytoplasm 0.0165 0.0012 0.0049 0.0005 0.0007 0.0005 0.0015 0.0001 0.0006 0.0003 0.0002 0.0005 0.0045 0.0002 0.0004 0.0023 0.0001 0.0003
Cytoplasmic Foci 0.0378 0.0017 0.0058 0.0149 0.0189 0.002 0.0231 0.001 0.0075 0.01 0.0105 0.0018 0.0173 0.0009 0.0074 0.0024 0.0005 0.0005
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003 0.0004 0 0.0002 0.0002 0.0001 0.0001 0.0007 0.0001 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.009 0.0011 0.0025 0.0016 0.0003 0.0037 0.0042 0.0008 0.0015 0.0027 0.0004 0.0011 0.0098 0.0014 0.0034 0.0012 0.0012 0.0021
Endosome 0.0333 0.0024 0.0037 0.0071 0.0046 0.0158 0.0372 0.002 0.0127 0.0105 0.0467 0.0076 0.0213 0.0021 0.0028 0.0194 0.0016 0.0073
Golgi 0.0129 0.0023 0.003 0.0033 0.0066 0.0048 0.022 0.0013 0.0108 0.0139 0.0305 0.0048 0.0108 0.0015 0.0044 0.002 0.0013 0.0033
Lipid Particles 0.058 0.0376 0.0356 0.0552 0.0503 0.0282 0.1099 0.023 0.0469 0.0767 0.1968 0.0422 0.0625 0.0203 0.0249 0.0587 0.0304 0.0128
Mitochondria 0.009 0.0042 0.0021 0.0075 0.0015 0.034 0.0206 0.0014 0.0127 0.0211 0.0086 0.0052 0.0075 0.002 0.0074 0.0023 0.0064 0.0147
None 0.0098 0.0008 0.0141 0.0007 0.0006 0.0008 0.0019 0.0005 0.0013 0.0015 0.0003 0.0005 0.0072 0.0006 0.0008 0.0014 0.0007 0.0003
Nuclear Periphery 0.634 0.7593 0.8404 0.7998 0.5951 0.7382 0.6549 0.8742 0.8041 0.7159 0.57 0.8671 0.7144 0.8519 0.7846 0.7675 0.8577 0.8697
Nucleolus 0.0217 0.021 0.0069 0.0212 0.0313 0.0378 0.0038 0.0236 0.0088 0.034 0.0402 0.0149 0.0121 0.0286 0.027 0.013 0.0142 0.0207
Nucleus 0.029 0.0944 0.0296 0.0471 0.0213 0.0555 0.0039 0.0219 0.0256 0.0104 0.0607 0.0139 0.0141 0.0262 0.0331 0.0287 0.0522 0.0335
Peroxisomes 0.0172 0.0017 0.0069 0.0104 0.0529 0.003 0.0239 0.0004 0.0038 0.0272 0.0031 0.0008 0.0173 0.0007 0.0109 0.001 0.0005 0.0002
Punctate Nuclear 0.0423 0.0483 0.0222 0.0141 0.1752 0.0475 0.0374 0.0282 0.0204 0.0321 0.0147 0.0221 0.0214 0.0423 0.039 0.0604 0.0123 0.0201
Vacuole 0.0024 0.0012 0.0007 0.0035 0.0004 0.0015 0.0021 0.0009 0.0025 0.0018 0.0039 0.0006 0.002 0.0009 0.0009 0.0207 0.0007 0.0008
Vacuole Periphery 0.0122 0.0158 0.0142 0.0079 0.0049 0.0108 0.0148 0.0197 0.0219 0.0349 0.0107 0.0101 0.0171 0.0174 0.0158 0.0154 0.0186 0.0081

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4477 16.6588 19.1929 22.0071 18.7569 11.273 22.1628 21.8392 19.5984 24.8207
Translational Efficiency 1.7591 1.3579 1.0443 0.9177 1.0999 1.8077 1.011 0.9403 1.0832 0.9146

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1962 301 136 1701 1986 1006 663 127 3948 1307 799 1828

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.09 842.23 1043.50 987.62 738.33 778.04 963.52 1068.38 693.48 792.82 977.13 993.23
Standard Deviation 76.69 105.54 147.54 140.90 90.57 112.14 111.75 142.36 95.32 113.91 122.36 142.49
Intensity Change Log 2 — 0.378020 0.687165 0.607762 — 0.075579 0.384049 0.533087 — 0.224870 0.533691 0.568476

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.000121 0.000672 0.000138 0.000073 0.000240 0.000488 0.000274 0.000066 0.000212 0.000520 0.000147
Bud Neck 0.000605 0.000118 0.002340 0.000813 0.000155 0.000360 0.000725 0.001378 0.000379 0.000304 0.001000 0.000853
Bud Site 0.000223 0.000043 0.000365 0.000331 0.000050 0.000310 0.000189 0.000236 0.000136 0.000248 0.000219 0.000325
Cell Periphery 0.000051 0.000020 0.000041 0.000019 0.000024 0.000057 0.000032 0.000021 0.000037 0.000049 0.000034 0.000019
Cytoplasm 0.025257 0.000787 0.000272 0.000527 0.002349 0.002344 0.002320 0.000517 0.013734 0.001986 0.001971 0.000526
Cytoplasmic Foci 0.022044 0.003508 0.000378 0.001754 0.003015 0.007439 0.001158 0.002938 0.012471 0.006533 0.001025 0.001836
Eisosomes 0.000027 0.000006 0.000012 0.000003 0.000010 0.000018 0.000024 0.000006 0.000019 0.000016 0.000022 0.000003
Endoplasmic Reticulum 0.002712 0.002918 0.006972 0.001655 0.002180 0.004071 0.004020 0.002587 0.002445 0.003806 0.004522 0.001720
Endosome 0.006234 0.002256 0.001048 0.002202 0.002805 0.005520 0.001135 0.001677 0.004509 0.004768 0.001120 0.002166
Golgi 0.003940 0.001631 0.000253 0.001075 0.001040 0.003774 0.000175 0.006457 0.002481 0.003281 0.000188 0.001449
Lipid Particles 0.307720 0.068371 0.001669 0.001996 0.085858 0.120685 0.003182 0.002094 0.196115 0.108637 0.002924 0.002003
Mitochondria 0.008471 0.001230 0.000869 0.001622 0.003522 0.005012 0.001335 0.001054 0.005981 0.004141 0.001255 0.001583
Mitotic Spindle 0.022307 0.033533 0.151482 0.210071 0.014187 0.038852 0.135207 0.300502 0.018222 0.037627 0.137978 0.216353
None 0.011727 0.000218 0.000268 0.000157 0.002035 0.002479 0.001953 0.000097 0.006852 0.001958 0.001666 0.000153
Nuclear Periphery 0.031944 0.041064 0.195288 0.158808 0.039738 0.036314 0.115791 0.164045 0.035865 0.037408 0.129323 0.159172
Nuclear Periphery Foci 0.512026 0.834531 0.536250 0.602844 0.813860 0.744674 0.576693 0.487044 0.663860 0.765368 0.569809 0.594799
Nucleolus 0.023194 0.005022 0.041259 0.005446 0.017262 0.012859 0.067024 0.012870 0.020210 0.011054 0.062639 0.005962
Nucleus 0.017562 0.003940 0.058684 0.006785 0.010492 0.011003 0.086405 0.012683 0.014006 0.009376 0.081687 0.007195
Peroxisomes 0.002229 0.000372 0.000704 0.000098 0.000607 0.002444 0.000699 0.000467 0.001413 0.001967 0.000700 0.000124
Vacuole 0.001152 0.000189 0.000769 0.002407 0.000409 0.000860 0.001252 0.001693 0.000778 0.000705 0.001170 0.002357
Vacuole Periphery 0.000515 0.000124 0.000406 0.001249 0.000327 0.000684 0.000192 0.001361 0.000420 0.000555 0.000228 0.001257

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.24 -5.05 -2.54 -0.76 4.11 -4.87 -9.02 -3.19 -1.67 1.75 -5.46 -10.36 -3.01 0.92 7.36
Bud Neck 5.96 -2.88 -1.92 -7.38 2.56 -3.34 -8.60 -2.43 -2.20 -1.44 1.31 -5.95 -4.91 -5.57 1.98
Bud Site 1.96 -1.32 -1.16 -4.17 0.78 -2.19 -3.94 -2.29 -1.19 -0.34 -1.19 -3.51 -2.91 -0.98 1.46
Cell Periphery 6.06 1.29 5.81 0.24 2.99 -2.34 -4.90 -0.97 1.60 1.75 -0.97 -1.93 3.91 2.51 4.67
Cytoplasm 12.15 12.71 12.57 0.49 -1.41 -0.17 -2.26 -0.27 -0.22 0.78 10.41 6.22 12.58 2.73 3.56
Cytoplasmic Foci 8.44 15.08 13.90 0.88 -3.10 -3.41 1.58 0.39 2.31 -0.33 6.00 11.98 12.97 4.80 0.12
Eisosomes 12.65 7.86 18.37 3.01 5.42 -3.53 -6.84 0.32 2.12 5.41 2.45 -4.18 18.16 7.91 9.24
Endoplasmic Reticulum -0.52 -3.50 3.60 1.93 4.21 -5.06 -6.41 -1.53 2.28 3.33 -4.38 -6.92 2.97 5.87 8.12
Endosome 5.13 9.00 6.68 0.06 -2.93 -3.13 1.55 0.82 3.43 -0.26 -0.38 6.32 5.21 3.91 -1.01
Golgi 2.47 7.67 5.38 0.58 -2.80 -3.27 5.69 -0.86 -0.39 -1.01 -0.90 8.91 2.27 2.31 -2.41
Lipid Particles 21.28 44.65 44.61 7.58 -0.90 -4.28 20.30 20.52 17.20 1.90 12.17 44.29 44.82 18.80 3.40
Mitochondria 8.64 10.90 7.17 -0.50 -1.12 -1.57 4.60 3.29 3.76 0.59 2.31 11.01 5.94 2.81 -0.38
Mitotic Spindle -1.50 -6.43 -24.71 -17.26 -2.33 -6.25 -13.03 -9.31 -8.44 -4.94 -5.56 -14.21 -27.23 -22.49 -6.17
None 10.56 10.50 10.60 0.80 1.52 -0.50 -1.22 5.83 3.87 4.30 6.81 5.49 11.73 3.92 4.36
Nuclear Periphery -2.40 -9.33 -30.72 -21.44 1.87 1.42 -11.52 -7.31 -7.52 -3.08 -0.97 -15.06 -31.41 -29.41 -5.88
Nuclear Periphery Foci -22.12 -1.21 -9.62 15.82 -2.94 6.24 19.51 11.91 9.16 2.34 -11.02 9.36 7.13 16.08 -4.05
Nucleolus 8.58 -1.61 10.49 -0.13 4.31 2.02 -9.73 0.44 -0.36 7.23 5.87 -9.27 10.96 3.54 13.02
Nucleus 7.33 -4.10 7.12 -1.81 5.30 -0.48 -11.56 -1.51 -1.32 8.51 3.01 -11.82 5.45 1.39 12.88
Peroxisomes 6.79 5.62 8.25 2.64 4.32 -3.17 -2.84 0.10 3.03 1.89 -0.87 2.79 9.29 4.20 7.31
Vacuole 3.15 0.92 -2.57 -4.90 -3.43 -1.99 -2.87 -2.12 -1.66 0.30 -0.02 -2.52 -3.83 -3.71 0.49
Vacuole Periphery 4.03 0.74 -2.44 -3.76 -2.73 -1.77 1.37 -0.99 -0.62 -1.10 -0.93 2.57 -2.91 -2.18 -3.50
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
Localization
Cell Percentages nuclear periphery (60%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mad1

Mad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available