Standard name
Human Ortholog
Description Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.06 0 0.05 0.05 0 0 0 0 0.05 0.09 0.14 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.16 0.07 0.14 0 0.12 0.09 0.07 0.06 0 0 0 0.05 0.11 0.19 0.2 0.36 0.29 0.22 0.18 0.16 0.2 0.16 0.19 0.25
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.59 0.65 0.32 0.52 0.37 0.57 0.6 0.48 0.79 0.79 0.79 0.6 0.54 0.29 0.35 0.11 0.15 0.12 0 0.05 0.07 0.08 0.06 0.05
Nucleus 0.05 0 0.08 0.13 0.07 0.06 0.13 0.15 0.09 0.08 0.12 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.46 0.43 0.32 0.37 0.44 0.36 0.27 0.21 0.23 0.23 0.23 0.3 0.44 0.55 0.36 0.46 0.43 0.49 0.57 0.57 0.54 0.55 0.5 0.46
Nucleolus 0.22 0.12 0 0.06 0.06 0.09 0.06 0.08 0.13 0.15 0.16 0.14 0.25 0.16 0.14 0.15 0.21 0.15 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
SpindlePole 0 0 0.13 0.05 0.06 0.05 0.06 0.12 0.07 0.05 0 0.1 0 0 0 0.05 0.06 0 0.09 0.07 0 0.05 0.08 0.08
Vac/Vac Membrane 0.14 0.15 0.18 0.06 0.08 0.05 0.06 0.08 0 0 0 0.05 0.07 0.11 0.09 0.26 0.32 0.33 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 3 1 1 0 2 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0 3 5
Bud 0 1 2 0 1 5 5 7 5 7 9 10 4 0 2 0 1 1 2 1 1 2 3 4
Bud Neck 0 0 5 0 2 0 1 2 0 0 0 2 0 0 0 0 0 0 0 0 2 0 1 2
Bud Site 0 1 1 1 1 2 5 5 7 5 10 8 1 0 4 0 0 0
Cell Periphery 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 2 10 12 14 15 18 19 10 6 8 9 16 32 46 5 3 5 0 0 1 0 0 0
Endoplasmic Reticulum 1 4 3 3 2 3 0 0 0 0 1 0 2 5 2 4 3 0 0 0 2 0 2 2
Endosome 24 16 30 8 27 32 27 23 1 3 4 19 33 69 64 42 21 18 29 41 66 22 72 86
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 2 0 3 2
Mitochondria 87 151 67 99 80 201 231 172 236 294 319 236 164 105 116 13 11 10 7 12 22 11 22 18
Nucleus 7 10 17 24 15 22 50 53 28 29 48 43 17 5 7 4 1 2 3 3 2 1 2 0
Nuclear Periphery 68 101 66 71 97 128 104 75 70 84 92 116 132 200 119 53 31 40 92 145 180 75 191 160
Nucleolus 33 28 5 12 13 31 24 30 38 56 66 54 75 58 46 17 15 12 2 5 13 5 12 2
Peroxisomes 0 2 1 1 3 5 7 2 2 3 0 2 2 7 5 0 0 2 2 5 5 1 20 19
SpindlePole 2 8 26 10 14 18 23 44 21 18 18 39 9 9 14 6 4 2 14 18 14 7 30 27
Vac/Vac Membrane 21 34 38 12 18 19 22 27 7 14 7 18 20 41 31 30 23 27 4 12 8 4 7 7
Unique Cell Count 147 233 207 191 218 352 385 358 298 372 404 391 303 363 327 116 72 81 163 253 335 138 379 349
Labelled Cell Count 249 359 275 254 289 482 519 460 425 519 582 557 475 532 457 176 114 120 163 253 335 138 379 349


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.7 5.6 5.1 5.7 4.6 4.8 5.1 4.1 4.0 3.9 4.6 5.3 5.6 5.6 8.5 8.4 8.2 5.2 5.2 5.5
Std Deviation (1e-4) 0.8 0.8 1.1 1.1 1.4 1.0 1.3 1.0 1.0 0.9 0.8 1.0 1.0 1.3 1.5 1.8 1.9 1.6 1.2 1.1 1.4
Intensity Change (Log2) -0.14 0.04 -0.27 -0.21 -0.12 -0.43 -0.46 -0.51 -0.27 -0.07 0.0 0.0 0.61 0.6 0.56 -0.09 -0.09 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 1.6 2.4 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1114 1.402 1.1617 0.8538 1.3225 0.9803 1.6508 1.8126 1.9806 1.5488 1.9427 1.4406 1.8027 1.444 1.3574 1.5185 0.803 1.2461
Actin 0.0321 0.0052 0.0063 0.0018 0.0335 0.0136 0.0353 0.0005 0.0109 0.0049 0.0013 0.0056 0.0551 0.002 0.0253 0.0018 0.001 0.0042
Bud 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0.0004 0 0.0004 0.0001 0.0001 0.0001 0.0007 0.0001 0.0004 0.0002 0 0.0001
Bud Neck 0.0137 0.0003 0.0002 0.0011 0.0007 0.0011 0.0004 0.0001 0.0005 0.0005 0.0005 0.0003 0.0004 0.0001 0.0024 0.0002 0.0001 0.001
Bud Periphery 0.0006 0.0004 0.0002 0.0003 0.0003 0.0005 0.0009 0.0002 0.0008 0.0005 0.0003 0.0003 0.0012 0.0003 0.0014 0.0003 0.0002 0.0003
Bud Site 0.0074 0.0008 0.0004 0.0015 0.0004 0.0004 0.0011 0.0001 0.0058 0.0006 0.0003 0.0004 0.0023 0.0004 0.0063 0.0009 0.0001 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0.0003 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0009 0.0001 0.0001 0.0001
Cytoplasm 0.0165 0.0012 0.0049 0.0005 0.0007 0.0005 0.0015 0.0001 0.0006 0.0003 0.0002 0.0005 0.0045 0.0002 0.0004 0.0023 0.0001 0.0003
Cytoplasmic Foci 0.0378 0.0017 0.0058 0.0149 0.0189 0.002 0.0231 0.001 0.0075 0.01 0.0105 0.0018 0.0173 0.0009 0.0074 0.0024 0.0005 0.0005
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003 0.0004 0 0.0002 0.0002 0.0001 0.0001 0.0007 0.0001 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.009 0.0011 0.0025 0.0016 0.0003 0.0037 0.0042 0.0008 0.0015 0.0027 0.0004 0.0011 0.0098 0.0014 0.0034 0.0012 0.0012 0.0021
Endosome 0.0333 0.0024 0.0037 0.0071 0.0046 0.0158 0.0372 0.002 0.0127 0.0105 0.0467 0.0076 0.0213 0.0021 0.0028 0.0194 0.0016 0.0073
Golgi 0.0129 0.0023 0.003 0.0033 0.0066 0.0048 0.022 0.0013 0.0108 0.0139 0.0305 0.0048 0.0108 0.0015 0.0044 0.002 0.0013 0.0033
Lipid Particles 0.058 0.0376 0.0356 0.0552 0.0503 0.0282 0.1099 0.023 0.0469 0.0767 0.1968 0.0422 0.0625 0.0203 0.0249 0.0587 0.0304 0.0128
Mitochondria 0.009 0.0042 0.0021 0.0075 0.0015 0.034 0.0206 0.0014 0.0127 0.0211 0.0086 0.0052 0.0075 0.002 0.0074 0.0023 0.0064 0.0147
None 0.0098 0.0008 0.0141 0.0007 0.0006 0.0008 0.0019 0.0005 0.0013 0.0015 0.0003 0.0005 0.0072 0.0006 0.0008 0.0014 0.0007 0.0003
Nuclear Periphery 0.634 0.7593 0.8404 0.7998 0.5951 0.7382 0.6549 0.8742 0.8041 0.7159 0.57 0.8671 0.7144 0.8519 0.7846 0.7675 0.8577 0.8697
Nucleolus 0.0217 0.021 0.0069 0.0212 0.0313 0.0378 0.0038 0.0236 0.0088 0.034 0.0402 0.0149 0.0121 0.0286 0.027 0.013 0.0142 0.0207
Nucleus 0.029 0.0944 0.0296 0.0471 0.0213 0.0555 0.0039 0.0219 0.0256 0.0104 0.0607 0.0139 0.0141 0.0262 0.0331 0.0287 0.0522 0.0335
Peroxisomes 0.0172 0.0017 0.0069 0.0104 0.0529 0.003 0.0239 0.0004 0.0038 0.0272 0.0031 0.0008 0.0173 0.0007 0.0109 0.001 0.0005 0.0002
Punctate Nuclear 0.0423 0.0483 0.0222 0.0141 0.1752 0.0475 0.0374 0.0282 0.0204 0.0321 0.0147 0.0221 0.0214 0.0423 0.039 0.0604 0.0123 0.0201
Vacuole 0.0024 0.0012 0.0007 0.0035 0.0004 0.0015 0.0021 0.0009 0.0025 0.0018 0.0039 0.0006 0.002 0.0009 0.0009 0.0207 0.0007 0.0008
Vacuole Periphery 0.0122 0.0158 0.0142 0.0079 0.0049 0.0108 0.0148 0.0197 0.0219 0.0349 0.0107 0.0101 0.0171 0.0174 0.0158 0.0154 0.0186 0.0081

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4477 16.6588 19.1929 22.0071 18.7569 11.273 22.1628 21.8392 19.5984 24.8207
Translational Efficiency 1.7591 1.3579 1.0443 0.9177 1.0999 1.8077 1.011 0.9403 1.0832 0.9146

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency