Standard name
Description RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0 0 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.11 0.14 0.05 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.44 0.34 0.27 0.35 0.25 0.31 0.25 0.12 0.3 0.26 0.2 0.29 0.36 0.38 0.27 0.07 0.07 0.06 0 0 0 0 0 0
Nucleus 0.19 0.26 0.2 0.1 0.07 0.07 0.07 0.06 0.07 0.08 0 0.09 0.16 0.06 0.06 0.14 0.16 0.17 0.12 0.18 0.1 0.06 0.05 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.92 0.85 0.87 0.91 0.94 0.92 0.89 0.91 0.84 0.85 0.93 0.84 0.85 0.86 0.81 0.78 0.81 0.81 0.81 0.74 0.82 0.85 0.83 0.74
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0.05 0.13 0.14 0.12 0.18 0.19 0.14 0 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0
Bud 1 0 0 0 0 1 2 2 2 3 3 3 0 1 3 0 0 0 2 1 2 11 8 21
Bud Neck 0 0 2 0 0 0 1 2 2 1 1 1 0 0 1 0 0 0 0 0 0 1 3 6
Bud Site 0 0 1 0 0 4 7 5 20 16 2 10 2 3 8 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 0 3 7 4 3 7 5 5 7 6 5 48 67 109 8 4 7 0 2 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 1
Endosome 0 2 0 0 4 1 12 17 0 5 0 1 5 9 12 0 1 0 0 2 2 1 8 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 6 4
Mitochondria 96 97 70 67 80 108 120 74 107 138 31 45 279 355 271 4 5 5 1 1 0 4 8 9
Nucleus 41 75 53 19 21 23 32 40 24 42 6 14 124 53 61 8 12 14 27 53 26 44 54 109
Nuclear Periphery 0 2 0 2 0 0 1 1 6 0 0 0 0 0 1 0 0 0 0 0 0 2 4 3
Nucleolus 198 246 226 173 302 318 435 561 302 445 143 129 651 803 810 46 61 68 179 216 208 607 910 797
Peroxisomes 0 0 2 1 2 3 6 8 8 5 1 1 0 7 8 0 0 0 0 0 0 1 1 0
SpindlePole 0 0 1 5 15 18 62 89 43 93 30 21 5 21 37 0 0 0 4 5 6 12 50 28
Vac/Vac Membrane 2 3 2 0 0 1 1 5 1 2 0 1 9 10 12 0 2 0 2 7 6 10 27 63
Unique Cell Count 216 289 259 190 321 347 488 619 361 521 154 154 770 930 1006 59 75 84 221 293 256 713 1093 1073
Labelled Cell Count 339 426 360 274 428 480 686 809 520 758 223 231 1124 1330 1334 67 86 94 221 293 256 713 1093 1073


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.1 21.8 21.8 22.1 17.8 13.6 11.3 10.4 9.6 8.7 8.1 7.7 32.5 29.3 26.7 35.8 39.3 47.3 31.7 28.6 30.8
Std Deviation (1e-4) 9.8 7.6 7.6 7.8 5.2 3.6 3.4 2.9 3.6 2.8 2.8 3.6 12.9 12.6 12.5 20.5 20.0 24.2 10.3 9.1 10.0
Intensity Change (Log2) 0.02 -0.29 -0.68 -0.95 -1.07 -1.18 -1.33 -1.44 -1.5 0.58 0.42 0.29 0.71 0.85 1.12 0.54 0.39 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.5 2.4 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 3.3 3.7 4.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 2.7 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 -0.6 1.1 -0.7 -5.5 0.7 -0.2 -1.6 0.5 2.7 3.3 0 -3.3 -3.7 -4.1
Nucleus -3.0 -5.0 -5.1 -5.7 -6.1 -5.1 -5.0 -4.7 -3.0 -1.6 -7.4 -7.2 -1.2 -0.9 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.3 2.9 1.8 0.8 1.5 -1.2 -0.7 1.8 -1.0 -1.1 -0.4 -2.5 -1.8 -1.3 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.1 3.4 5.8 6.2 5.5 7.1 7.1 5.8 0 0 2.8 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dbp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available