Standard name
Human Ortholog
Description Trimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationally regulated; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.07 – 0.07 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.07 0.08 0.06 0.07 0.05 – 0.09 0.05 0 0.1 0.09 0.15 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.09 0.09 0.11 0.08 0.26 0.31 0.25 0.59 – 0.54 0.39 0.22 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.95 0.89 0.94 0.89 0.88 0.81 0.85 0.82 0.79 – 0.75 0.82 0.94 0.87 0.86 0.78 0.81 0.8 0.93 0.88 0.84 0.63 0.65 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.07 – 0.11 0.06 0.11 0 0 0 0.1 0.05 0 0 0 0.16 0.15 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0.05 0.06 0.09 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 1 0 0 2 1 6 1 10 – 2 8 0 0 0 0 0 0 1 3 2 2 5 0
Bud Neck 0 0 2 2 1 1 1 3 0 – 1 5 0 0 0 0 0 0 0 0 1 1 2 1
Bud Site 0 0 2 4 3 6 18 6 19 – 8 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 9 16 16 23 22 23 13 – 10 10 5 34 29 10 5 5 0 3 5 0 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 – 0 0 0 1 0 5 1 1 0 0 1 2 0 1
Endosome 0 1 2 1 1 1 1 2 0 – 0 0 0 1 1 0 0 1 1 1 1 3 4 10
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 1 0 0 0 0 0 0
Mitochondria 16 22 30 19 20 78 119 88 155 – 61 85 26 8 12 3 3 4 0 2 4 1 2 4
Nucleus 166 211 318 158 209 247 328 289 207 – 86 180 109 301 263 52 59 47 164 219 296 88 164 121
Nuclear Periphery 0 3 2 1 2 0 3 7 6 – 1 0 0 1 3 0 1 0 0 0 0 0 0 0
Nucleolus 4 3 4 3 4 6 16 9 18 – 12 13 13 6 8 1 7 3 0 3 14 22 36 39
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 13 5 4 8 4 16 3 – 6 6 1 1 2 0 1 1 1 1 1 4 1 9
Vac/Vac Membrane 2 7 2 1 0 3 1 1 0 – 1 2 1 5 5 0 4 1 5 11 20 12 27 23
Unique Cell Count 174 236 337 177 237 305 385 353 261 114 220 116 346 306 67 73 59 176 250 354 141 252 221
Labelled Cell Count 191 256 384 210 262 374 519 445 431 188 321 155 360 323 73 81 64 176 250 354 141 252 221


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.8 5.6 5.3 5.8 4.8 5.0 5.0 4.2 – 4.1 4.1 5.5 5.7 5.7 7.7 9.0 8.5 5.1 5.4 5.7
Std Deviation (1e-4) 0.7 1.3 0.9 1.5 1.5 1.3 1.5 1.3 1.5 – 1.5 0.9 1.1 1.2 1.6 1.4 2.0 2.0 1.1 1.1 1.5
Intensity Change (Log2) – – – -0.08 0.05 -0.22 -0.17 -0.16 -0.41 – -0.44 -0.44 -0.03 0.02 0.02 0.47 0.68 0.6 -0.13 -0.05 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.3 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 3.2 2.4 2.8 2.0 2.4 1.5 – 0 1.2 0 3.8 3.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.7 -0.2 0 7.3 5.6 0 – 0 0 3.8 -3.8 -2.6 -1.2 -1.4 -0.5
Nucleus -2.1 -2.7 -5.2 -4.0 -5.0 -5.6 – -5.7 -4.7 -0.2 -3.3 -3.6 -4.5 -3.9 -3.9
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.4 1.3 0 – 0 0 0 0 1.3 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole -0.6 -1.5 0 -2.5 0.4 0 – 0 0 0 -3.3 -2.7 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1314 3.9814 3.3048 3.2047 2.3818 3.1349 2.1364 2.3468 1.9486 1.8091 2.0213 1.8786 1.4904 1.0863 1.3538 0.9621 2.0994 1.0055
Actin 0.0293 0 0.0141 0 0.0589 0.0001 0.0094 0.0002 0.004 0.0039 0.0081 0.0052 0.0072 0 0.0108 0.0234 0.0322 0.0059
Bud 0.0003 0 0.0001 0 0.0001 0 0.0006 0 0.0004 0.0006 0.0019 0.0004 0.0001 0 0.0003 0.0006 0.0002 0.0002
Bud Neck 0.0023 0 0.0005 0 0.0003 0.0008 0.0021 0.0001 0.0006 0.0004 0.0013 0.0011 0.0003 0.0001 0.0004 0.0004 0.0009 0.0008
Bud Periphery 0.0006 0 0.0001 0 0.0002 0.0001 0.0014 0 0.0007 0.0011 0.0081 0.0009 0.0002 0 0.0002 0.0007 0.0003 0.0006
Bud Site 0.0038 0 0.0009 0 0.0003 0 0.0026 0.0001 0.0033 0.002 0.0044 0.0013 0.0004 0 0.0031 0.0052 0.001 0.0004
Cell Periphery 0.0005 0 0 0 0 0 0.0003 0 0.0004 0.0001 0.0007 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0035 0.0001 0.0015 0 0.0002 0.0002 0.0086 0.0163 0.0028 0.0007 0.0004 0.0015 0.0161 0.0002 0.0028 0.0006 0.0005 0.0001
Cytoplasmic Foci 0.0077 0 0.0069 0 0.001 0.0001 0.0157 0.0011 0.0111 0.0039 0.01 0.0008 0.0125 0 0.0122 0.0039 0.0071 0.0009
Eisosomes 0.0012 0 0.0002 0 0.0002 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0026 0 0.0004 0 0.0002 0 0.0024 0.001 0.002 0.0005 0.0006 0.0007 0.0102 0 0.0005 0.0008 0.0006 0.0001
Endosome 0.0162 0 0.0045 0 0.0004 0.0002 0.0238 0.0016 0.0174 0.0377 0.0171 0.0023 0.0179 0 0.0035 0.0118 0.007 0.0022
Golgi 0.0075 0 0.0039 0 0.001 0.0001 0.0059 0.0001 0.011 0.0047 0.0099 0.0011 0.0039 0 0.0032 0.0074 0.0068 0.0035
Lipid Particles 0.0128 0 0.0092 0 0.0006 0.002 0.0219 0.0003 0.0062 0.0051 0.0401 0.0011 0.0075 0 0.0014 0.0047 0.0335 0.0051
Mitochondria 0.0063 0.0001 0.0073 0 0.0005 0.0074 0.0101 0.0002 0.0298 0.0038 0.0505 0.0092 0.0041 0.0001 0.0034 0.0043 0.0189 0.0168
None 0.0049 0 0.0049 0 0.0008 0.0089 0.0133 0.0025 0.0003 0.0004 0.0003 0.0002 0.0104 0.0001 0.0003 0.0006 0.0018 0
Nuclear Periphery 0.0037 0.0012 0.0028 0.0002 0.0054 0.0005 0.0236 0.0023 0.0046 0.0065 0.0065 0.0072 0.0093 0.0004 0.0012 0.0015 0.0017 0.0002
Nucleolus 0.0124 0.0084 0.0075 0.0047 0.0296 0.0479 0.0205 0.0118 0.0101 0.0065 0.051 0.032 0.0112 0.012 0.0071 0.0129 0.0255 0.0328
Nucleus 0.8648 0.9899 0.9187 0.9948 0.8933 0.9291 0.818 0.9601 0.882 0.9109 0.7366 0.9323 0.8731 0.9867 0.9437 0.916 0.8394 0.9277
Peroxisomes 0.0089 0 0.0068 0 0.0023 0.0004 0.0044 0.0001 0.0075 0.0021 0.0093 0.0007 0.0029 0 0.0048 0.0029 0.0153 0.0011
Punctate Nuclear 0.0057 0.0002 0.0091 0.0002 0.0045 0.0017 0.0076 0.0016 0.0008 0.0058 0.0042 0.0009 0.0048 0.0003 0.0009 0.0012 0.0063 0.0004
Vacuole 0.0035 0.0001 0.0003 0 0.0001 0.0001 0.0051 0.0004 0.0038 0.0021 0.0206 0.0006 0.0055 0 0.0002 0.0005 0.0003 0.0005
Vacuole Periphery 0.0015 0 0.0002 0 0.0001 0.0001 0.0024 0.0001 0.0009 0.0011 0.0183 0.0005 0.0021 0 0.0001 0.0004 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9943 12.6155 14.9195 22.9602 12.2934 15.8675 22.7208 23.6751 22.5397 21.5236
Translational Efficiency 0.9153 1.1573 0.9989 0.5855 1.0337 1.1701 0.709 0.7449 0.8326 0.7742

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Trimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationally regulated; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hsf1

Hsf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available