Standard name
Description Trimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationally regulated; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.07 – 0.07 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.07 0.08 0.06 0.07 0.05 – 0.09 0.05 0 0.1 0.09 0.15 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.09 0.09 0.11 0.08 0.26 0.31 0.25 0.59 – 0.54 0.39 0.22 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.95 0.89 0.94 0.89 0.88 0.81 0.85 0.82 0.79 – 0.75 0.82 0.94 0.87 0.86 0.78 0.81 0.8 0.93 0.88 0.84 0.63 0.65 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.07 – 0.11 0.06 0.11 0 0 0 0.1 0.05 0 0 0 0.16 0.15 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0.05 0.06 0.09 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 1 0 0 2 1 6 1 10 – 2 8 0 0 0 0 0 0 1 3 2 2 5 0
Bud Neck 0 0 2 2 1 1 1 3 0 – 1 5 0 0 0 0 0 0 0 0 1 1 2 1
Bud Site 0 0 2 4 3 6 18 6 19 – 8 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 9 16 16 23 22 23 13 – 10 10 5 34 29 10 5 5 0 3 5 0 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 – 0 0 0 1 0 5 1 1 0 0 1 2 0 1
Endosome 0 1 2 1 1 1 1 2 0 – 0 0 0 1 1 0 0 1 1 1 1 3 4 10
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 1 0 0 0 0 0 0
Mitochondria 16 22 30 19 20 78 119 88 155 – 61 85 26 8 12 3 3 4 0 2 4 1 2 4
Nucleus 166 211 318 158 209 247 328 289 207 – 86 180 109 301 263 52 59 47 164 219 296 88 164 121
Nuclear Periphery 0 3 2 1 2 0 3 7 6 – 1 0 0 1 3 0 1 0 0 0 0 0 0 0
Nucleolus 4 3 4 3 4 6 16 9 18 – 12 13 13 6 8 1 7 3 0 3 14 22 36 39
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 13 5 4 8 4 16 3 – 6 6 1 1 2 0 1 1 1 1 1 4 1 9
Vac/Vac Membrane 2 7 2 1 0 3 1 1 0 – 1 2 1 5 5 0 4 1 5 11 20 12 27 23
Unique Cell Count 174 236 337 177 237 305 385 353 261 114 220 116 346 306 67 73 59 176 250 354 141 252 221
Labelled Cell Count 191 256 384 210 262 374 519 445 431 188 321 155 360 323 73 81 64 176 250 354 141 252 221


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.8 5.6 5.3 5.8 4.8 5.0 5.0 4.2 – 4.1 4.1 5.5 5.7 5.7 7.7 9.0 8.5 5.1 5.4 5.7
Std Deviation (1e-4) 0.7 1.3 0.9 1.5 1.5 1.3 1.5 1.3 1.5 – 1.5 0.9 1.1 1.2 1.6 1.4 2.0 2.0 1.1 1.1 1.5
Intensity Change (Log2) – – – -0.08 0.05 -0.22 -0.17 -0.16 -0.41 – -0.44 -0.44 -0.03 0.02 0.02 0.47 0.68 0.6 -0.13 -0.05 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.3 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 3.2 2.4 2.8 2.0 2.4 1.5 – 0 1.2 0 3.8 3.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.7 -0.2 0 7.3 5.6 0 – 0 0 3.8 -3.8 -2.6 -1.2 -1.4 -0.5
Nucleus -2.1 -2.7 -5.2 -4.0 -5.0 -5.6 – -5.7 -4.7 -0.2 -3.3 -3.6 -4.5 -3.9 -3.9
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.4 1.3 0 – 0 0 0 0 1.3 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole -0.6 -1.5 0 -2.5 0.4 0 – 0 0 0 -3.3 -2.7 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0