Standard name | |
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Description | Trimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationally regulated; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 1 | 0 | 0 | 2 | 1 | 6 | 1 | 10 | – | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 5 | 0 |
Bud Neck | 0 | 0 | 2 | 2 | 1 | 1 | 1 | 3 | 0 | – | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 2 | 4 | 3 | 6 | 18 | 6 | 19 | – | 8 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | 9 | 16 | 16 | 23 | 22 | 23 | 13 | – | 10 | 10 | 5 | 34 | 29 | 10 | 5 | 5 | 0 | 3 | 5 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 5 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 1 |
Endosome | 0 | 1 | 2 | 1 | 1 | 1 | 1 | 2 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 3 | 4 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 16 | 22 | 30 | 19 | 20 | 78 | 119 | 88 | 155 | – | 61 | 85 | 26 | 8 | 12 | 3 | 3 | 4 | 0 | 2 | 4 | 1 | 2 | 4 |
Nucleus | 166 | 211 | 318 | 158 | 209 | 247 | 328 | 289 | 207 | – | 86 | 180 | 109 | 301 | 263 | 52 | 59 | 47 | 164 | 219 | 296 | 88 | 164 | 121 |
Nuclear Periphery | 0 | 3 | 2 | 1 | 2 | 0 | 3 | 7 | 6 | – | 1 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 3 | 4 | 3 | 4 | 6 | 16 | 9 | 18 | – | 12 | 13 | 13 | 6 | 8 | 1 | 7 | 3 | 0 | 3 | 14 | 22 | 36 | 39 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 13 | 5 | 4 | 8 | 4 | 16 | 3 | – | 6 | 6 | 1 | 1 | 2 | 0 | 1 | 1 | 1 | 1 | 1 | 4 | 1 | 9 |
Vac/Vac Membrane | 2 | 7 | 2 | 1 | 0 | 3 | 1 | 1 | 0 | – | 1 | 2 | 1 | 5 | 5 | 0 | 4 | 1 | 5 | 11 | 20 | 12 | 27 | 23 |
Unique Cell Count | 174 | 236 | 337 | 177 | 237 | 305 | 385 | 353 | 261 | 114 | 220 | 116 | 346 | 306 | 67 | 73 | 59 | 176 | 250 | 354 | 141 | 252 | 221 | |
Labelled Cell Count | 191 | 256 | 384 | 210 | 262 | 374 | 519 | 445 | 431 | 188 | 321 | 155 | 360 | 323 | 73 | 81 | 64 | 176 | 250 | 354 | 141 | 252 | 221 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.8 | 5.6 | 5.3 | 5.8 | 4.8 | 5.0 | 5.0 | 4.2 | – | 4.1 | 4.1 | 5.5 | 5.7 | 5.7 | 7.7 | 9.0 | 8.5 | 5.1 | 5.4 | 5.7 |
Std Deviation (1e-4) | 0.7 | 1.3 | 0.9 | 1.5 | 1.5 | 1.3 | 1.5 | 1.3 | 1.5 | – | 1.5 | 0.9 | 1.1 | 1.2 | 1.6 | 1.4 | 2.0 | 2.0 | 1.1 | 1.1 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.05 | -0.22 | -0.17 | -0.16 | -0.41 | – | -0.44 | -0.44 | -0.03 | 0.02 | 0.02 | 0.47 | 0.68 | 0.6 | -0.13 | -0.05 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 3.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.2 | 2.4 | 2.8 | 2.0 | 2.4 | 1.5 | – | 0 | 1.2 | 0 | 3.8 | 3.7 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.7 | -0.2 | 0 | 7.3 | 5.6 | 0 | – | 0 | 0 | 3.8 | -3.8 | -2.6 | -1.2 | -1.4 | -0.5 |
Nucleus | -2.1 | -2.7 | -5.2 | -4.0 | -5.0 | -5.6 | – | -5.7 | -4.7 | -0.2 | -3.3 | -3.6 | -4.5 | -3.9 | -3.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.4 | 1.3 | 0 | – | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.6 | -1.5 | 0 | -2.5 | 0.4 | 0 | – | 0 | 0 | 0 | -3.3 | -2.7 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |