Standard name
Description Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.06 0.07 0.09 0.05 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.98 0.94 0.91 0.83 0.86 0.84 0.83 0.83 0.99 0.98 0.98 0.92 0.91 0.97 0.98 0.97 0.93 0.95 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.15 0.09 0.12 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 3 2 2 1 5 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 2 1 0 0 1 2 0 0 0 0 0 1 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 7 2 0 0 7 9 19 33 17 33 3 15 1 0 3 3 5 2 0 0 0 0 0 0
Cytoplasm 372 94 188 196 314 263 388 429 215 312 50 114 219 186 196 228 127 176 364 92 182 125 162 251
Endoplasmic Reticulum 1 1 1 1 3 9 32 78 23 44 5 8 3 2 3 7 3 8 2 0 4 0 2 11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 1 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 1 0 0 1 0 0 2 4 4 8 0 0 1 1 0 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 14 7 3 0 0 3 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 3 0 1 1 2 1 1 0 2 0 6 2 2 0 0 2 0 2 2
Unique Cell Count 374 95 190 197 319 280 425 519 251 370 60 138 221 190 201 249 140 182 372 95 197 132 173 277
Labelled Cell Count 380 97 190 197 324 286 440 547 261 399 64 155 225 191 203 260 147 193 372 95 197 132 173 277

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 109.1 96.1 76.7 69.6 76.3 68.7 64.3 59.0 52.2 52.8 46.2 48.4 99.5 91.9 77.1 113.4 130.8 117.8 125.6 115.3 121.1
Std Deviation (1e-4) 26.1 24.0 21.9 19.3 20.0 18.8 17.5 17.0 16.9 16.2 19.9 22.8 28.5 27.5 25.9 58.9 63.4 54.9 38.5 38.1 40.8
Intensity Change (Log2) -0.14 -0.01 -0.16 -0.25 -0.38 -0.55 -0.54 -0.73 -0.66 0.38 0.26 0.01 0.56 0.77 0.62 0.71 0.59 0.66

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.0 3.6 3.7 4.2 0 4.7 0 0 0 0 0 0
Cytoplasm 4.4 6.6 0.2 3.8 1.7 3.5 2.1 2.1 2.6 4.4 4.2 3.2 -5.2 -2.7 -4.2
Endoplasmic Reticulum 0 0 0 3.6 5.4 4.0 4.7 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0