Standard name
Description Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.06 0.07 0.07 – 0.06 0 0.06 0.05 0 0.1 0.18 0 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.1 0.12 0.08 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 – 0.08 0.06 0.12 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.91 0.91 0.92 0.79 0.71 0.76 – 0.75 0.71 0.64 0.66 0.9 0.81 0.67 0.66 0.63 0.51 0.88 0.91 0.89 0.85 0.77 0.66
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.11 0 0 0 0.08 0 – 0.07 0.1 0.14 0.14 0.07 0 0.05 0 0 0 0.05 0 0 0.06 0.11 0.11
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.05 0 0.14 0.15 0.15 – 0.12 0.14 0.14 0.16 0 0.07 0.11 0.24 0.27 0.37 0 0 0 0.05 0.08 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 0 2 – 0 2 2 2 0 0 0 0 0 0 3 0 1 2 4 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 – 1 2 6 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 5 3 6 9 19 20 32 – 21 11 26 22 5 35 53 10 9 23 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 5 6 6 0 0 0 0 0 0
Endosome 0 0 0 1 1 0 1 – 4 6 9 12 1 0 2 0 8 1 1 1 1 0 1 1
Golgi 0 0 1 0 8 0 22 – 1 9 1 0 0 4 2 24 29 21 1 0 0 0 1 2
Mitochondria 0 1 0 1 0 25 5 – 27 21 50 42 0 0 0 1 1 1 0 0 2 0 0 0
Nucleus 152 81 119 231 253 199 362 – 248 237 274 313 214 271 201 160 155 141 177 97 122 145 265 193
Nuclear Periphery 0 0 0 1 1 1 1 – 4 0 1 2 0 0 0 1 0 0 0 0 0 0 1 0
Nucleolus 27 10 4 5 5 23 13 – 23 32 58 65 16 8 14 6 3 9 9 3 4 9 37 31
Peroxisomes 0 0 0 0 0 0 0 – 0 1 0 0 0 0 3 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 3 – 1 0 0 1 0 0 2 0 2 0 0 0 0 0 1 3
Vac/Vac Membrane 10 4 6 9 44 41 72 – 41 46 61 76 10 23 33 59 65 103 3 2 3 7 27 52
Unique Cell Count 182 89 131 251 322 280 477 331 336 427 476 239 335 299 243 245 275 201 107 137 171 343 291
Labelled Cell Count 194 99 136 258 331 311 513 371 367 489 540 246 341 311 267 278 306 201 107 137 171 343 291


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 17.5 13.8 14.5 17.1 10.0 14.6 – 12.3 12.7 11.4 11.7 19.3 18.6 19.2 28.3 26.8 26.5 14.2 15.7 15.5
Std Deviation (1e-4) 6.2 8.3 5.2 5.4 6.1 4.4 6.4 – 5.4 5.2 4.9 5.0 6.7 7.5 7.8 8.9 8.7 8.9 6.2 7.0 7.5
Intensity Change (Log2) – – – 0.08 0.32 -0.46 0.09 – -0.16 -0.12 -0.27 -0.24 0.49 0.43 0.48 1.04 0.96 0.95 0.04 0.19 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 0.6 1.0 0.9 – 0.7 0 0.6 0 0 2.0 3.6 -0.2 -0.4 1.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 3.4 3.8 2.9
Mitochondria 0 0 3.5 0 – 3.4 2.9 4.1 3.5 0 0 0 0 0 0
Nucleus 0.4 -3.1 -4.5 -3.7 – -3.8 -4.6 -5.9 -5.6 -0.4 -2.6 -5.2 -5.3 -5.7 -7.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.0 0 – 1.6 2.4 3.4 3.4 1.5 0 0.8 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -0.5 2.8 3.0 3.2 – 2.5 2.8 3.0 3.4 -0.2 0.9 2.1 4.8 5.2 7.0