Standard name | |
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Description | Pheromone-regulated protein; contains with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family; PRM8 has a paralog, PRM9, that arose from a segmental duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 3 |
Bud | – | 0 | 0 | 0 | 6 | 6 | 22 | 11 | 9 | 9 | 14 | 16 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 1 | 0 | 2 | 1 | 0 | 2 | 0 | 2 | 0 | 2 | 3 | 3 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 28 | 56 | 144 | 266 | 318 | 558 | 566 | 473 | 499 | 342 | 538 | 298 | 328 | 394 | 314 | 220 | 222 | 5 | 26 | 21 | 49 | 133 | 127 |
Endoplasmic Reticulum | – | 0 | 2 | 4 | 11 | 7 | 1 | 5 | 6 | 3 | 3 | 10 | 3 | 21 | 27 | 11 | 5 | 6 | 12 | 2 | 8 | 3 | 6 | 4 |
Endosome | – | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 1 | 4 | 0 | 7 | 0 | 0 | 1 | 8 | 1 | 2 | 1 | 2 | 4 | 0 | 10 | 5 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Mitochondria | – | 0 | 5 | 10 | 25 | 23 | 49 | 30 | 66 | 36 | 48 | 68 | 0 | 0 | 0 | 6 | 3 | 2 | 0 | 0 | 3 | 5 | 12 | 14 |
Nucleus | – | 0 | 0 | 0 | 2 | 1 | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | – | 0 | 0 | 1 | 1 | 0 | 3 | 3 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 3 |
Vac/Vac Membrane | – | 4 | 4 | 6 | 7 | 11 | 34 | 37 | 30 | 54 | 28 | 32 | 2 | 3 | 10 | 72 | 33 | 55 | 19 | 43 | 50 | 72 | 233 | 219 |
Unique Cell Count | 31 | 61 | 159 | 298 | 346 | 615 | 618 | 527 | 564 | 375 | 609 | 305 | 353 | 423 | 377 | 246 | 262 | 43 | 80 | 92 | 139 | 418 | 392 | |
Labelled Cell Count | 33 | 67 | 167 | 320 | 368 | 679 | 661 | 590 | 606 | 440 | 676 | 311 | 359 | 434 | 417 | 264 | 291 | 43 | 80 | 92 | 139 | 418 | 392 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 6.4 | 4.3 | 4.2 | 4.3 | 4.4 | 4.1 | 4.4 | 4.2 | 4.5 | 4.3 | 4.6 | 5.4 | 5.7 | 6.0 | 5.9 | 6.2 | 6.5 | 5.6 | 5.7 | 5.6 |
Std Deviation (1e-4) | – | 1.7 | 0.6 | 0.7 | 1.3 | 1.0 | 0.9 | 1.0 | 0.8 | 0.9 | 0.9 | 1.0 | 0.8 | 0.8 | 1.0 | 1.3 | 1.3 | 1.6 | 1.0 | 1.0 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.02 | 0.03 | -0.06 | 0.04 | -0.01 | 0.06 | 0.01 | 0.1 | 0.34 | 0.42 | 0.49 | 0.46 | 0.54 | 0.61 | 0.39 | 0.42 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | -0.6 | 0 | -0.3 | -0.1 | -0.5 | -0.8 | -0.2 | -0.8 | 2.4 | 0.3 | 0.4 | -1.7 | -0.6 | -1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 2.4 | 1.5 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0741 | 1.735 | 1.5898 | 1.0876 | 0.8083 | 1.3639 | 1.0199 | 2.0068 | 1.1443 | 1.2322 | 1.1227 | 1.3685 | -0.3 | 0.9318 | 0.3318 | 0.069 | 0.6765 | 0.3694 |
Actin | 0.0106 | 0.0012 | 0.0165 | 0.0158 | 0.0001 | 0.0045 | 0.0016 | 0.0001 | 0.0086 | 0.0043 | 0.0095 | 0.0008 | 0.0007 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0005 |
Bud | 0.0004 | 0.0003 | 0.0009 | 0.0011 | 0.0001 | 0.0003 | 0.0052 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0007 | 0.0005 | 0.0022 | 0.0259 | 0.0005 | 0.0009 | 0.0036 | 0.0003 | 0.0008 | 0.0016 | 0.0007 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0127 |
Bud Periphery | 0.0003 | 0.0003 | 0.0016 | 0.0016 | 0.0001 | 0.0004 | 0.0035 | 0.0004 | 0.0006 | 0.0006 | 0.0009 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0034 | 0.0021 | 0.0084 | 0.0169 | 0.0002 | 0.0009 | 0.0049 | 0.0108 | 0.002 | 0.0024 | 0.0018 | 0.0002 | 0.0005 | 0.001 | 0.0008 | 0.0003 | 0.0001 | 0.0011 |
Cell Periphery | 0.0004 | 0.0005 | 0.0007 | 0.0006 | 0.0001 | 0.0002 | 0.0013 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0006 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.3831 | 0.4434 | 0.3499 | 0.3741 | 0.5036 | 0.5208 | 0.264 | 0.4147 | 0.3163 | 0.2331 | 0.3479 | 0.4151 | 0.3775 | 0.4597 | 0.4512 | 0.3756 | 0.4387 | 0.4789 |
Cytoplasmic Foci | 0.0608 | 0.0414 | 0.045 | 0.0809 | 0.0322 | 0.03 | 0.0618 | 0.0471 | 0.0415 | 0.109 | 0.0565 | 0.0496 | 0.0372 | 0.0232 | 0.0298 | 0.0241 | 0.0387 | 0.0282 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0454 | 0.0452 | 0.0394 | 0.0366 | 0.0152 | 0.0321 | 0.0772 | 0.0827 | 0.0276 | 0.0053 | 0.027 | 0.0287 | 0.1301 | 0.134 | 0.0982 | 0.0895 | 0.1523 | 0.164 |
Endosome | 0.1058 | 0.1128 | 0.1446 | 0.2062 | 0.1632 | 0.0915 | 0.116 | 0.0881 | 0.1681 | 0.2119 | 0.1477 | 0.1264 | 0.1118 | 0.1081 | 0.1266 | 0.1338 | 0.176 | 0.1137 |
Golgi | 0.0091 | 0.002 | 0.0089 | 0.0134 | 0.0015 | 0.0058 | 0.006 | 0.0028 | 0.004 | 0.0096 | 0.0167 | 0.0037 | 0.0018 | 0.0023 | 0.0012 | 0.001 | 0.0011 | 0.0011 |
Lipid Particles | 0.0277 | 0.0076 | 0.0086 | 0.0132 | 0.0013 | 0.0087 | 0.0159 | 0.0112 | 0.0065 | 0.0116 | 0.0194 | 0.0067 | 0.0173 | 0.0076 | 0.0077 | 0.0021 | 0.0029 | 0.0035 |
Mitochondria | 0.0024 | 0.0015 | 0.008 | 0.0071 | 0.0009 | 0.0048 | 0.008 | 0.0015 | 0.0122 | 0.0018 | 0.0346 | 0.0039 | 0.0007 | 0.0014 | 0.0005 | 0.0007 | 0.0004 | 0.0009 |
None | 0.2331 | 0.2133 | 0.2457 | 0.0923 | 0.2305 | 0.1984 | 0.3072 | 0.2415 | 0.3021 | 0.2222 | 0.1631 | 0.2728 | 0.2011 | 0.1175 | 0.1713 | 0.1501 | 0.0651 | 0.0895 |
Nuclear Periphery | 0.0102 | 0.0117 | 0.0119 | 0.0075 | 0.0042 | 0.0102 | 0.0195 | 0.0099 | 0.0078 | 0.0024 | 0.0077 | 0.0076 | 0.0218 | 0.0209 | 0.0155 | 0.023 | 0.0215 | 0.0227 |
Nucleolus | 0.0017 | 0.0007 | 0.0013 | 0.007 | 0.0003 | 0.0004 | 0.0017 | 0.0007 | 0.0005 | 0.0006 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0082 | 0.006 | 0.0059 | 0.0099 | 0.0047 | 0.0077 | 0.0071 | 0.0044 | 0.0035 | 0.0022 | 0.0048 | 0.0042 | 0.0045 | 0.0063 | 0.005 | 0.0046 | 0.0036 | 0.0079 |
Peroxisomes | 0.0127 | 0.0013 | 0.0063 | 0.0031 | 0.0001 | 0.0079 | 0.0169 | 0.0183 | 0.001 | 0.0444 | 0.0374 | 0.0009 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.013 | 0.0066 | 0.0074 | 0.0056 | 0.0014 | 0.0039 | 0.0028 | 0.0053 | 0.0009 | 0.003 | 0.0016 | 0.001 | 0.0027 | 0.0014 | 0.0012 | 0.0006 | 0.0009 | 0.001 |
Vacuole | 0.0657 | 0.0969 | 0.0798 | 0.0747 | 0.035 | 0.0651 | 0.0699 | 0.0554 | 0.0873 | 0.1226 | 0.1095 | 0.0697 | 0.0855 | 0.1034 | 0.0827 | 0.1754 | 0.0885 | 0.0652 |
Vacuole Periphery | 0.005 | 0.0049 | 0.007 | 0.0064 | 0.0047 | 0.0054 | 0.0054 | 0.004 | 0.0077 | 0.0105 | 0.0114 | 0.0068 | 0.0045 | 0.0114 | 0.0064 | 0.0178 | 0.0096 | 0.0085 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.6354 | 17.9592 | 20.5023 | 26.4489 | 20.0366 | 18.5343 | 23.3966 | 34.4105 | 23.6213 | 30.5636 |
Translational Efficiency | 0.3264 | 0.4838 | 0.3966 | 0.5943 | 0.5049 | 0.6158 | 0.5259 | 0.4066 | 0.8788 | 0.5999 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |