Standard name
Description Pheromone-regulated protein; contains with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family; PRM8 has a paralog, PRM9, that arose from a segmental duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.9 0.92 0.91 0.89 0.92 0.91 0.92 0.9 0.88 0.91 0.88 0.98 0.93 0.93 0.83 0.89 0.85 0.13 0.33 0.23 0.36 0.32 0.33
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0.29 0 0.1 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0.08 0.06 0.08 0.07 0.08 0.05 0.13 0.06 0.13 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.13 0.07 0 0 0 0.06 0.06 0.06 0.1 0.07 0.05 0 0 0 0.19 0.13 0.21 0.46 0.54 0.55 0.52 0.56 0.56
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 1 0 2 1 0 0 1 1 0 2 0 0 0 0 1 1 0 2 1 3
Bud – 0 0 0 6 6 22 11 9 9 14 16 4 1 1 0 0 0 0 0 0 0 3 5
Bud Neck – 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 2 2
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 0 2 1 0 2 0 2 0 2 3 3 3 0 0 0 1 0 0 0 0 0 0
Cytoplasm – 28 56 144 266 318 558 566 473 499 342 538 298 328 394 314 220 222 5 26 21 49 133 127
Endoplasmic Reticulum – 0 2 4 11 7 1 5 6 3 3 10 3 21 27 11 5 6 12 2 8 3 6 4
Endosome – 0 0 0 0 2 3 2 1 4 0 7 0 0 1 8 1 2 1 2 4 0 10 5
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0
Mitochondria – 0 5 10 25 23 49 30 66 36 48 68 0 0 0 6 3 2 0 0 3 5 12 14
Nucleus – 0 0 0 2 1 2 4 1 0 0 0 0 0 0 5 1 2 0 0 0 0 0 1
Nuclear Periphery – 0 0 1 1 0 3 3 2 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 1 3 3
Vac/Vac Membrane – 4 4 6 7 11 34 37 30 54 28 32 2 3 10 72 33 55 19 43 50 72 233 219
Unique Cell Count 31 61 159 298 346 615 618 527 564 375 609 305 353 423 377 246 262 43 80 92 139 418 392
Labelled Cell Count 33 67 167 320 368 679 661 590 606 440 676 311 359 434 417 264 291 43 80 92 139 418 392


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 6.4 4.3 4.2 4.3 4.4 4.1 4.4 4.2 4.5 4.3 4.6 5.4 5.7 6.0 5.9 6.2 6.5 5.6 5.7 5.6
Std Deviation (1e-4) – 1.7 0.6 0.7 1.3 1.0 0.9 1.0 0.8 0.9 0.9 1.0 0.8 0.8 1.0 1.3 1.3 1.6 1.0 1.0 1.1
Intensity Change (Log2) – – – -0.04 0.02 0.03 -0.06 0.04 -0.01 0.06 0.01 0.1 0.34 0.42 0.49 0.46 0.54 0.61 0.39 0.42 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -0.6 0 -0.3 -0.1 -0.5 -0.8 -0.2 -0.8 2.4 0.3 0.4 -1.7 -0.6 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0.8 0 0 0 0 0 2.4 1.5 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0741 1.735 1.5898 1.0876 0.8083 1.3639 1.0199 2.0068 1.1443 1.2322 1.1227 1.3685 -0.3 0.9318 0.3318 0.069 0.6765 0.3694
Actin 0.0106 0.0012 0.0165 0.0158 0.0001 0.0045 0.0016 0.0001 0.0086 0.0043 0.0095 0.0008 0.0007 0.0003 0.0002 0.0001 0 0.0005
Bud 0.0004 0.0003 0.0009 0.0011 0.0001 0.0003 0.0052 0.0003 0.0004 0.0005 0.0006 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0007 0.0005 0.0022 0.0259 0.0005 0.0009 0.0036 0.0003 0.0008 0.0016 0.0007 0.0006 0.0001 0.0002 0.0002 0.0003 0.0002 0.0127
Bud Periphery 0.0003 0.0003 0.0016 0.0016 0.0001 0.0004 0.0035 0.0004 0.0006 0.0006 0.0009 0.0003 0.0001 0.0003 0.0001 0.0001 0 0.0001
Bud Site 0.0034 0.0021 0.0084 0.0169 0.0002 0.0009 0.0049 0.0108 0.002 0.0024 0.0018 0.0002 0.0005 0.001 0.0008 0.0003 0.0001 0.0011
Cell Periphery 0.0004 0.0005 0.0007 0.0006 0.0001 0.0002 0.0013 0.0004 0.0005 0.0003 0.0005 0.0003 0.0006 0.0005 0.0006 0.0002 0.0001 0.0002
Cytoplasm 0.3831 0.4434 0.3499 0.3741 0.5036 0.5208 0.264 0.4147 0.3163 0.2331 0.3479 0.4151 0.3775 0.4597 0.4512 0.3756 0.4387 0.4789
Cytoplasmic Foci 0.0608 0.0414 0.045 0.0809 0.0322 0.03 0.0618 0.0471 0.0415 0.109 0.0565 0.0496 0.0372 0.0232 0.0298 0.0241 0.0387 0.0282
Eisosomes 0.0001 0 0.0002 0.0002 0 0 0.0007 0 0.0001 0.0001 0.0002 0.0002 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0454 0.0452 0.0394 0.0366 0.0152 0.0321 0.0772 0.0827 0.0276 0.0053 0.027 0.0287 0.1301 0.134 0.0982 0.0895 0.1523 0.164
Endosome 0.1058 0.1128 0.1446 0.2062 0.1632 0.0915 0.116 0.0881 0.1681 0.2119 0.1477 0.1264 0.1118 0.1081 0.1266 0.1338 0.176 0.1137
Golgi 0.0091 0.002 0.0089 0.0134 0.0015 0.0058 0.006 0.0028 0.004 0.0096 0.0167 0.0037 0.0018 0.0023 0.0012 0.001 0.0011 0.0011
Lipid Particles 0.0277 0.0076 0.0086 0.0132 0.0013 0.0087 0.0159 0.0112 0.0065 0.0116 0.0194 0.0067 0.0173 0.0076 0.0077 0.0021 0.0029 0.0035
Mitochondria 0.0024 0.0015 0.008 0.0071 0.0009 0.0048 0.008 0.0015 0.0122 0.0018 0.0346 0.0039 0.0007 0.0014 0.0005 0.0007 0.0004 0.0009
None 0.2331 0.2133 0.2457 0.0923 0.2305 0.1984 0.3072 0.2415 0.3021 0.2222 0.1631 0.2728 0.2011 0.1175 0.1713 0.1501 0.0651 0.0895
Nuclear Periphery 0.0102 0.0117 0.0119 0.0075 0.0042 0.0102 0.0195 0.0099 0.0078 0.0024 0.0077 0.0076 0.0218 0.0209 0.0155 0.023 0.0215 0.0227
Nucleolus 0.0017 0.0007 0.0013 0.007 0.0003 0.0004 0.0017 0.0007 0.0005 0.0006 0.0004 0.0003 0.0004 0.0002 0.0003 0.0002 0.0001 0.0001
Nucleus 0.0082 0.006 0.0059 0.0099 0.0047 0.0077 0.0071 0.0044 0.0035 0.0022 0.0048 0.0042 0.0045 0.0063 0.005 0.0046 0.0036 0.0079
Peroxisomes 0.0127 0.0013 0.0063 0.0031 0.0001 0.0079 0.0169 0.0183 0.001 0.0444 0.0374 0.0009 0.0006 0.0001 0.0002 0.0001 0.0001 0.0001
Punctate Nuclear 0.013 0.0066 0.0074 0.0056 0.0014 0.0039 0.0028 0.0053 0.0009 0.003 0.0016 0.001 0.0027 0.0014 0.0012 0.0006 0.0009 0.001
Vacuole 0.0657 0.0969 0.0798 0.0747 0.035 0.0651 0.0699 0.0554 0.0873 0.1226 0.1095 0.0697 0.0855 0.1034 0.0827 0.1754 0.0885 0.0652
Vacuole Periphery 0.005 0.0049 0.007 0.0064 0.0047 0.0054 0.0054 0.004 0.0077 0.0105 0.0114 0.0068 0.0045 0.0114 0.0064 0.0178 0.0096 0.0085

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.6354 17.9592 20.5023 26.4489 20.0366 18.5343 23.3966 34.4105 23.6213 30.5636
Translational Efficiency 0.3264 0.4838 0.3966 0.5943 0.5049 0.6158 0.5259 0.4066 0.8788 0.5999

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘