Standard name
Description GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0.08 0.15 0.14 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.13 0.07 0.2 0.17 0.28 0.41 – 0.45 0.37 0 0 0.07 – – – 0 0 0 0 0 0
Nucleus 0.93 0.94 0.94 0.92 0.92 0.88 0.87 0.8 0.79 – 0.76 0.75 0.89 0.83 0.77 – – – 0.86 0.89 0.87 0.88 0.81 0.72
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.08 0.1 0.13 – 0.13 0.15 0 0 0.05 – – – 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0.07 0.05 0.09 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 1 1 0 0 1 1
Bud 0 0 0 0 1 1 2 6 1 – 5 1 0 0 0 – – – 2 2 2 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 1 1 13 13 20 15 – 20 19 0 0 2 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 6 7 7 9 17 18 22 19 10 – 17 15 24 50 53 – – – 1 1 0 2 0 2
Endoplasmic Reticulum 0 1 0 1 0 1 0 0 1 – 0 0 0 0 1 – – – 0 0 1 0 0 1
Endosome 2 0 0 0 0 0 0 3 0 – 0 0 0 0 0 – – – 2 3 2 0 4 3
Golgi 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 – – – 3 1 0 0 1 5
Mitochondria 2 11 48 46 27 107 105 239 210 – 208 161 9 8 26 – – – 1 3 3 2 1 5
Nucleus 204 270 368 321 363 477 546 693 399 – 348 329 254 280 286 – – – 222 260 352 213 216 240
Nuclear Periphery 1 0 1 4 2 11 24 20 8 – 9 14 1 3 3 – – – 0 1 0 0 0 1
Nucleolus 5 7 6 6 16 24 50 84 65 – 58 66 4 11 17 – – – 11 6 8 7 11 15
Peroxisomes 1 0 0 0 0 0 0 0 0 – 0 1 3 0 4 – – – 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 1 2 0 – 1 1 3 2 4 – – – 1 2 1 0 0 3
Vac/Vac Membrane 4 5 6 3 2 7 5 12 7 – 5 17 1 2 11 – – – 8 4 27 11 23 45
Unique Cell Count 220 286 393 348 393 540 631 861 508 460 440 287 338 371 259 292 403 243 268 335
Labelled Cell Count 225 301 436 392 429 659 768 1098 716 672 624 299 356 407 259 292 403 243 268 335


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 7.1 5.7 5.8 6.0 5.4 5.3 4.9 4.5 – 4.5 4.9 6.8 6.6 6.1 – – – 7.3 7.5 7.9
Std Deviation (1e-4) 2.4 1.3 1.1 1.5 1.6 1.3 1.4 1.2 1.3 – 1.2 2.0 1.8 1.8 1.6 – – – 1.7 1.7 1.6
Intensity Change (Log2) – – – 0.04 0.09 -0.07 -0.11 -0.22 -0.32 – -0.32 -0.2 0.27 0.22 0.1 – – – 0.37 0.41 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud Site 0 0 3.1 0 0 0 – 0 4.2 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Cytoplasm 0.8 2.1 1.4 1.6 0.5 0.2 – 1.7 1.5 4.1 6.5 6.4 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Mitochondria 0.4 -2.5 3.1 1.9 0 0 – 0 8.1 -4.2 -5.0 -2.4 – – –
Nucleus -0.7 -0.7 -2.7 -3.6 -6.0 -6.3 – -7.1 -7.4 -2.4 -4.6 -6.5 – – –
Nuclear Periphery 0 0 2.4 3.6 0 0 – 0 3.2 0 0 0 – – –
Nucleolus 0.2 2.2 2.5 4.4 0 0 – 0 6.9 0 1.5 2.5 – – –
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Vacuole 0 0 -0.3 0 -0.2 -0.2 – -0.6 2.1 0 0 1.3 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9543 5.2354 4.5532 4.5698 4.3905 4.5369 2.3697 2.2604 2.2795 1.7681 1.9575 2.1749 6.6215 6.4155 6.3612 6.502 6.4533 6.545
Actin 0.0054 0 0.0041 0 0.001 0 0.0141 0 0.0029 0 0.0039 0 0 0 0 0 0 0
Bud 0.0001 0 0 0 0 0 0.0003 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Bud Neck 0.001 0 0.0002 0.0001 0.0001 0.0006 0.0008 0 0.0001 0.0001 0.0022 0.0004 0 0.0001 0.0001 0 0.0001 0.0002
Bud Periphery 0.0003 0 0.0001 0.0001 0.0001 0 0.0008 0 0.0001 0 0.0004 0 0 0 0 0 0 0
Bud Site 0.0006 0 0.0011 0 0 0 0.0005 0 0.0005 0 0.0007 0 0 0.0001 0 0 0 0
Cell Periphery 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.0006 0.0001 0.0008 0.0001 0.0003 0 0.0024 0.0001 0.0013 0 0.0006 0.0007 0.0001 0.0039 0 0 0 0
Cytoplasmic Foci 0.002 0 0.0018 0 0.0001 0 0.0009 0 0.0015 0 0.0036 0 0 0 0 0 0 0
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0007 0 0.0007 0.0001 0.0002 0 0.0016 0 0.0009 0 0.0013 0.0001 0 0.0005 0 0 0 0
Endosome 0.0033 0 0.002 0.0001 0.0011 0 0.0058 0 0.002 0 0.0127 0.0001 0 0.0002 0 0 0 0
Golgi 0.0008 0 0.001 0 0.0001 0 0.0031 0 0.0007 0 0.0057 0 0 0 0 0 0 0
Lipid Particles 0.0039 0 0.0038 0 0 0 0.0043 0 0.0024 0 0.0297 0 0 0 0 0 0 0
Mitochondria 0.0028 0.0001 0.001 0.0003 0.0008 0.0001 0.0222 0.0001 0.0051 0 0.0069 0.0001 0.0001 0.0003 0.0001 0 0 0
None 0.0006 0 0.0024 0 0.0002 0 0.0009 0 0.0005 0 0.0002 0 0 0.0001 0 0 0 0
Nuclear Periphery 0.0068 0.0004 0.0043 0.0024 0.0161 0.0003 0.0212 0.0047 0.0042 0.0002 0.0165 0.0008 0.0013 0.0022 0.0006 0.0004 0.0002 0.0003
Nucleolus 0.0133 0.0077 0.0062 0.009 0.03 0.0351 0.0185 0.0088 0.0072 0.0035 0.0174 0.0219 0.0071 0.0099 0.0061 0.0045 0.0247 0.0198
Nucleus 0.9521 0.9911 0.9651 0.9864 0.9483 0.9635 0.8935 0.9855 0.9647 0.9961 0.8902 0.9725 0.9883 0.9818 0.9927 0.9946 0.9745 0.9791
Peroxisomes 0.0016 0 0.0017 0 0 0 0.0021 0 0.0024 0 0.002 0 0 0 0 0 0 0
Punctate Nuclear 0.002 0.0004 0.0026 0.0005 0.0008 0.0002 0.0032 0.0005 0.0026 0.0001 0.0037 0.0023 0.003 0.0004 0.0003 0.0004 0.0005 0.0005
Vacuole 0.0011 0.0001 0.0005 0.0005 0.0002 0.0001 0.0012 0.0001 0.0004 0 0.0013 0.0008 0 0.0003 0.0001 0 0 0
Vacuole Periphery 0.0009 0 0.0004 0.0004 0.0006 0 0.0024 0.0001 0.0003 0 0.001 0.0001 0 0.0001 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.7631 14.6637 16.2007 18.0611 10.8702 12.3868 16.5562 16.9004 15.4963 17.4611
Translational Efficiency 0.843 0.6429 0.7453 0.7034 1.0075 0.7955 0.7117 0.5458 0.705 0.642

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘