Standard name | |
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Description | GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 1 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 6 | 1 | – | 5 | 1 | 0 | 0 | 0 | – | – | – | 2 | 2 | 2 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 13 | 13 | 20 | 15 | – | 20 | 19 | 0 | 0 | 2 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 7 | 7 | 9 | 17 | 18 | 22 | 19 | 10 | – | 17 | 15 | 24 | 50 | 53 | – | – | – | 1 | 1 | 0 | 2 | 0 | 2 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 2 | 3 | 2 | 0 | 4 | 3 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 1 | 0 | 0 | 1 | 5 |
Mitochondria | 2 | 11 | 48 | 46 | 27 | 107 | 105 | 239 | 210 | – | 208 | 161 | 9 | 8 | 26 | – | – | – | 1 | 3 | 3 | 2 | 1 | 5 |
Nucleus | 204 | 270 | 368 | 321 | 363 | 477 | 546 | 693 | 399 | – | 348 | 329 | 254 | 280 | 286 | – | – | – | 222 | 260 | 352 | 213 | 216 | 240 |
Nuclear Periphery | 1 | 0 | 1 | 4 | 2 | 11 | 24 | 20 | 8 | – | 9 | 14 | 1 | 3 | 3 | – | – | – | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 5 | 7 | 6 | 6 | 16 | 24 | 50 | 84 | 65 | – | 58 | 66 | 4 | 11 | 17 | – | – | – | 11 | 6 | 8 | 7 | 11 | 15 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 3 | 0 | 4 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | – | 1 | 1 | 3 | 2 | 4 | – | – | – | 1 | 2 | 1 | 0 | 0 | 3 |
Vac/Vac Membrane | 4 | 5 | 6 | 3 | 2 | 7 | 5 | 12 | 7 | – | 5 | 17 | 1 | 2 | 11 | – | – | – | 8 | 4 | 27 | 11 | 23 | 45 |
Unique Cell Count | 220 | 286 | 393 | 348 | 393 | 540 | 631 | 861 | 508 | 460 | 440 | 287 | 338 | 371 | 259 | 292 | 403 | 243 | 268 | 335 | ||||
Labelled Cell Count | 225 | 301 | 436 | 392 | 429 | 659 | 768 | 1098 | 716 | 672 | 624 | 299 | 356 | 407 | 259 | 292 | 403 | 243 | 268 | 335 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 7.1 | 5.7 | 5.8 | 6.0 | 5.4 | 5.3 | 4.9 | 4.5 | – | 4.5 | 4.9 | 6.8 | 6.6 | 6.1 | – | – | – | 7.3 | 7.5 | 7.9 |
Std Deviation (1e-4) | 2.4 | 1.3 | 1.1 | 1.5 | 1.6 | 1.3 | 1.4 | 1.2 | 1.3 | – | 1.2 | 2.0 | 1.8 | 1.8 | 1.6 | – | – | – | 1.7 | 1.7 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.09 | -0.07 | -0.11 | -0.22 | -0.32 | – | -0.32 | -0.2 | 0.27 | 0.22 | 0.1 | – | – | – | 0.37 | 0.41 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 3.1 | 0 | 0 | 0 | – | 0 | 4.2 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 0.8 | 2.1 | 1.4 | 1.6 | 0.5 | 0.2 | – | 1.7 | 1.5 | 4.1 | 6.5 | 6.4 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0.4 | -2.5 | 3.1 | 1.9 | 0 | 0 | – | 0 | 8.1 | -4.2 | -5.0 | -2.4 | – | – | – |
Nucleus | -0.7 | -0.7 | -2.7 | -3.6 | -6.0 | -6.3 | – | -7.1 | -7.4 | -2.4 | -4.6 | -6.5 | – | – | – |
Nuclear Periphery | 0 | 0 | 2.4 | 3.6 | 0 | 0 | – | 0 | 3.2 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0.2 | 2.2 | 2.5 | 4.4 | 0 | 0 | – | 0 | 6.9 | 0 | 1.5 | 2.5 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | -0.3 | 0 | -0.2 | -0.2 | – | -0.6 | 2.1 | 0 | 0 | 1.3 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9543 | 5.2354 | 4.5532 | 4.5698 | 4.3905 | 4.5369 | 2.3697 | 2.2604 | 2.2795 | 1.7681 | 1.9575 | 2.1749 | 6.6215 | 6.4155 | 6.3612 | 6.502 | 6.4533 | 6.545 |
Actin | 0.0054 | 0 | 0.0041 | 0 | 0.001 | 0 | 0.0141 | 0 | 0.0029 | 0 | 0.0039 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0022 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0006 | 0 | 0.0011 | 0 | 0 | 0 | 0.0005 | 0 | 0.0005 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0006 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0024 | 0.0001 | 0.0013 | 0 | 0.0006 | 0.0007 | 0.0001 | 0.0039 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.002 | 0 | 0.0018 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0.0015 | 0 | 0.0036 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0007 | 0 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0016 | 0 | 0.0009 | 0 | 0.0013 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0 |
Endosome | 0.0033 | 0 | 0.002 | 0.0001 | 0.0011 | 0 | 0.0058 | 0 | 0.002 | 0 | 0.0127 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 |
Golgi | 0.0008 | 0 | 0.001 | 0 | 0.0001 | 0 | 0.0031 | 0 | 0.0007 | 0 | 0.0057 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0039 | 0 | 0.0038 | 0 | 0 | 0 | 0.0043 | 0 | 0.0024 | 0 | 0.0297 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0028 | 0.0001 | 0.001 | 0.0003 | 0.0008 | 0.0001 | 0.0222 | 0.0001 | 0.0051 | 0 | 0.0069 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 |
None | 0.0006 | 0 | 0.0024 | 0 | 0.0002 | 0 | 0.0009 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0068 | 0.0004 | 0.0043 | 0.0024 | 0.0161 | 0.0003 | 0.0212 | 0.0047 | 0.0042 | 0.0002 | 0.0165 | 0.0008 | 0.0013 | 0.0022 | 0.0006 | 0.0004 | 0.0002 | 0.0003 |
Nucleolus | 0.0133 | 0.0077 | 0.0062 | 0.009 | 0.03 | 0.0351 | 0.0185 | 0.0088 | 0.0072 | 0.0035 | 0.0174 | 0.0219 | 0.0071 | 0.0099 | 0.0061 | 0.0045 | 0.0247 | 0.0198 |
Nucleus | 0.9521 | 0.9911 | 0.9651 | 0.9864 | 0.9483 | 0.9635 | 0.8935 | 0.9855 | 0.9647 | 0.9961 | 0.8902 | 0.9725 | 0.9883 | 0.9818 | 0.9927 | 0.9946 | 0.9745 | 0.9791 |
Peroxisomes | 0.0016 | 0 | 0.0017 | 0 | 0 | 0 | 0.0021 | 0 | 0.0024 | 0 | 0.002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.002 | 0.0004 | 0.0026 | 0.0005 | 0.0008 | 0.0002 | 0.0032 | 0.0005 | 0.0026 | 0.0001 | 0.0037 | 0.0023 | 0.003 | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0005 |
Vacuole | 0.0011 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0012 | 0.0001 | 0.0004 | 0 | 0.0013 | 0.0008 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 |
Vacuole Periphery | 0.0009 | 0 | 0.0004 | 0.0004 | 0.0006 | 0 | 0.0024 | 0.0001 | 0.0003 | 0 | 0.001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.7631 | 14.6637 | 16.2007 | 18.0611 | 10.8702 | 12.3868 | 16.5562 | 16.9004 | 15.4963 | 17.4611 |
Translational Efficiency | 0.843 | 0.6429 | 0.7453 | 0.7034 | 1.0075 | 0.7955 | 0.7117 | 0.5458 | 0.705 | 0.642 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |