Standard name
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0.06 0.05 0 0.05 0 0 0.12 0.12 0.13 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.97 0.94 0.93 0.9 0.75 0.83 0.79 0.77 0.77 0.72 0.74 0.93 0.84 0.8 0.68 0.66 0.68 0.9 0.92 0.75 0.86 0.84 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.12 0.05 0.11 0 0.08 0.07 0 0.09 0.12 0.15 0.13 0 0 0 0.08 0.12 0.08 0.06 0 0.17 0 0.08 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0.15 0.1 0.16 0.12 0.08 0.09 0.08 0 0 0.05 0.12 0.18 0.17 0 0.05 0.06 0.08 0.06 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 1 0 0 0 0 2 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 1 1 1 2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 5 1 5 4 8 11 8 5 7 4 6 25 27 32 9 9 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 4 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 3 5 0 0 0 0 0 0
Mitochondria 0 1 0 2 0 3 4 0 2 9 7 9 1 0 0 2 2 2 0 0 0 1 0 1
Nucleus 42 58 146 65 99 88 200 142 124 120 110 106 141 174 176 168 95 98 53 77 122 88 145 158
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 7 7 8 3 9 16 4 15 18 23 19 2 4 7 19 17 12 3 1 28 1 14 17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 3 3 1 4 18 24 28 19 13 13 11 4 5 10 29 26 24 0 4 9 8 10 32
Unique Cell Count 43 60 155 70 110 118 241 179 162 156 152 144 151 206 219 247 144 144 59 85 164 102 173 218
Labelled Cell Count 44 69 161 77 111 123 257 187 170 169 162 151 154 208 226 263 156 156 59 85 164 102 173 218


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.0 39.6 34.1 27.2 33.9 27.1 28.6 28.9 26.5 26.2 23.6 22.9 32.2 33.6 31.5 44.0 41.8 44.3 36.7 32.4 35.4
Std Deviation (1e-4) 6.7 9.9 10.0 7.1 13.8 6.4 7.2 8.2 7.7 7.8 7.6 6.9 9.8 13.4 12.4 17.4 14.7 18.3 13.8 10.4 11.9
Intensity Change (Log2) -0.33 -0.01 -0.33 -0.26 -0.24 -0.36 -0.38 -0.53 -0.58 -0.09 -0.02 -0.11 0.37 0.29 0.37 0.11 -0.07 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.1 1.2 0.8 0 0.6 0 0.4 3.0 3.1 3.3 1.2 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -0.4 -1.3 -4.6 -3.3 -3.9 -4.4 -4.3 -5.1 -4.9 -0.3 -2.9 -3.8 -6.2 -6.2 -5.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.1 0.9 -1.2 1.7 2.3 3.1 2.7 0 0 -0.7 1.3 2.3 1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 4.1 3.1 4.3 3.4 2.6 2.6 2.3 0 0 1.4 3.5 4.7 4.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpt6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available