Standard name | |
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Human Ortholog | |
Description | ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 5 | 1 | 5 | 4 | 8 | 11 | 8 | 5 | 7 | 4 | 6 | 25 | 27 | 32 | 9 | 9 | 0 | 0 | 0 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 2 | 0 | 3 | 4 | 0 | 2 | 9 | 7 | 9 | 1 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleus | 42 | 58 | 146 | 65 | 99 | 88 | 200 | 142 | 124 | 120 | 110 | 106 | 141 | 174 | 176 | 168 | 95 | 98 | 53 | 77 | 122 | 88 | 145 | 158 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 7 | 7 | 8 | 3 | 9 | 16 | 4 | 15 | 18 | 23 | 19 | 2 | 4 | 7 | 19 | 17 | 12 | 3 | 1 | 28 | 1 | 14 | 17 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 3 | 3 | 1 | 4 | 18 | 24 | 28 | 19 | 13 | 13 | 11 | 4 | 5 | 10 | 29 | 26 | 24 | 0 | 4 | 9 | 8 | 10 | 32 |
Unique Cell Count | 43 | 60 | 155 | 70 | 110 | 118 | 241 | 179 | 162 | 156 | 152 | 144 | 151 | 206 | 219 | 247 | 144 | 144 | 59 | 85 | 164 | 102 | 173 | 218 |
Labelled Cell Count | 44 | 69 | 161 | 77 | 111 | 123 | 257 | 187 | 170 | 169 | 162 | 151 | 154 | 208 | 226 | 263 | 156 | 156 | 59 | 85 | 164 | 102 | 173 | 218 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 30.0 | 39.6 | 34.1 | 27.2 | 33.9 | 27.1 | 28.6 | 28.9 | 26.5 | 26.2 | 23.6 | 22.9 | 32.2 | 33.6 | 31.5 | 44.0 | 41.8 | 44.3 | 36.7 | 32.4 | 35.4 |
Std Deviation (1e-4) | 6.7 | 9.9 | 10.0 | 7.1 | 13.8 | 6.4 | 7.2 | 8.2 | 7.7 | 7.8 | 7.6 | 6.9 | 9.8 | 13.4 | 12.4 | 17.4 | 14.7 | 18.3 | 13.8 | 10.4 | 11.9 |
Intensity Change (Log2) | – | – | – | -0.33 | -0.01 | -0.33 | -0.26 | -0.24 | -0.36 | -0.38 | -0.53 | -0.58 | -0.09 | -0.02 | -0.11 | 0.37 | 0.29 | 0.37 | 0.11 | -0.07 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0.1 | 1.2 | 0.8 | 0 | 0.6 | 0 | 0.4 | 3.0 | 3.1 | 3.3 | 1.2 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.4 | -1.3 | -4.6 | -3.3 | -3.9 | -4.4 | -4.3 | -5.1 | -4.9 | -0.3 | -2.9 | -3.8 | -6.2 | -6.2 | -5.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.1 | 0.9 | -1.2 | 1.7 | 2.3 | 3.1 | 2.7 | 0 | 0 | -0.7 | 1.3 | 2.3 | 1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 4.1 | 3.1 | 4.3 | 3.4 | 2.6 | 2.6 | 2.3 | 0 | 0 | 1.4 | 3.5 | 4.7 | 4.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.4155 | 28.451 | 23.5293 | 23.2515 | 22.3183 | 23.4284 | 20.6786 | 35.0579 | 29.7857 | 27.7065 | 26.541 | 27.3544 | 26.8813 | 34.6533 | 30.2471 | 32.0012 | 31.0414 | 31.1536 |
Actin | 0.0039 | 0.0001 | 0.0022 | 0.0006 | 0.0011 | 0.0004 | 0.0635 | 0.0001 | 0.0017 | 0.0002 | 0.0001 | 0.0021 | 0.0204 | 0 | 0.0031 | 0 | 0 | 0.0002 |
Bud | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0016 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0009 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.001 | 0.0011 | 0 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0002 |
Bud Periphery | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0021 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0014 | 0.001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Bud Site | 0.0008 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0022 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 |
Cytoplasm | 0.0208 | 0.0116 | 0.0022 | 0.0011 | 0.038 | 0.0003 | 0.0156 | 0.0214 | 0.002 | 0.0003 | 0.0004 | 0.0018 | 0.0278 | 0.0001 | 0.0022 | 0.0001 | 0 | 0 |
Cytoplasmic Foci | 0.0062 | 0.0001 | 0.0024 | 0 | 0.0011 | 0.0001 | 0.0027 | 0 | 0.0003 | 0 | 0.0001 | 0.0138 | 0.0305 | 0 | 0.0006 | 0 | 0 | 0.0011 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0062 | 0 | 0.0022 | 0.0001 | 0.0015 | 0.0002 | 0.0076 | 0 | 0.0004 | 0 | 0 | 0.0005 | 0.0052 | 0 | 0.0003 | 0 | 0 | 0 |
Endosome | 0.0089 | 0 | 0.0038 | 0.0001 | 0.0045 | 0.0006 | 0.0121 | 0 | 0.0027 | 0.0001 | 0 | 0.0054 | 0.0081 | 0 | 0.0025 | 0 | 0 | 0.0031 |
Golgi | 0.001 | 0 | 0.0005 | 0 | 0.0004 | 0.0002 | 0.0051 | 0 | 0.0001 | 0 | 0 | 0.0012 | 0.0024 | 0 | 0.0078 | 0 | 0 | 0.0094 |
Lipid Particles | 0.0028 | 0 | 0.0007 | 0 | 0.0003 | 0.0016 | 0.0029 | 0 | 0.0002 | 0 | 0 | 0.0045 | 0.0057 | 0 | 0.0009 | 0 | 0 | 0.0015 |
Mitochondria | 0.0038 | 0.0002 | 0.0009 | 0.0016 | 0.0014 | 0.0022 | 0.0102 | 0.0017 | 0.0255 | 0.008 | 0.0021 | 0.0199 | 0.0087 | 0.0007 | 0.0015 | 0.0035 | 0.0009 | 0.0021 |
None | 0.0029 | 0 | 0.0018 | 0.0001 | 0.0013 | 0.0001 | 0.0297 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0019 | 0.0083 | 0 | 0.0002 | 0 | 0 | 0 |
Nuclear Periphery | 0.0482 | 0.0065 | 0.0108 | 0.0399 | 0.0501 | 0.0106 | 0.061 | 0.0055 | 0.019 | 0.0031 | 0.0034 | 0.0128 | 0.0244 | 0.0043 | 0.0047 | 0.0039 | 0.0012 | 0.0007 |
Nucleolus | 0.0433 | 0.0117 | 0.0277 | 0.0028 | 0.1263 | 0.0895 | 0.0341 | 0.0207 | 0.0085 | 0.027 | 0.1362 | 0.0791 | 0.0345 | 0.0139 | 0.0114 | 0.042 | 0.0709 | 0.0273 |
Nucleus | 0.8268 | 0.9684 | 0.9268 | 0.9518 | 0.7668 | 0.8875 | 0.7327 | 0.9488 | 0.9353 | 0.9583 | 0.8525 | 0.8298 | 0.799 | 0.9806 | 0.9634 | 0.9467 | 0.926 | 0.953 |
Peroxisomes | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0004 | 0 | 0 | 0.0017 | 0.0016 | 0 | 0.0001 | 0 | 0 | 0.0006 |
Punctate Nuclear | 0.016 | 0.0011 | 0.0158 | 0.0008 | 0.002 | 0.0019 | 0.008 | 0.0007 | 0.0009 | 0.0005 | 0.0036 | 0.0142 | 0.0125 | 0 | 0.0004 | 0 | 0 | 0 |
Vacuole | 0.0029 | 0.0001 | 0.0005 | 0.0002 | 0.0017 | 0.0011 | 0.0026 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0021 | 0.0027 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0032 | 0.0001 | 0.0005 | 0.0007 | 0.0029 | 0.0015 | 0.0035 | 0.0007 | 0.0013 | 0.0007 | 0.0008 | 0.0054 | 0.0035 | 0.0002 | 0.0003 | 0.0021 | 0.0006 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 116.4101 | 110.1372 | 117.7824 | 111.7464 | 89.2664 | 97.7847 | 111.1549 | 113.293 | 125.7765 | 102.5871 |
Translational Efficiency | 1.7701 | 1.8374 | 1.5853 | 1.8957 | 1.8367 | 2.2573 | 1.7628 | 1.6028 | 1.4414 | 1.4463 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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