ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.08 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.98 1.0 0.98 0.96 0.94 0.95 0.89 – 0.8 0.82 1.0 0.99 0.99 0.95 0.94 0.93 0.9 0.9 0.89 0.68 0.53 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0.07 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.1 0.06 0.27 – 0.39 0.26 0 0 0 0 0 0 0 0 0 0.13 0.15 0.1
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.08 0.07 0 0 0 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 2 0 1 – 0 0 0 0 0 1 1 0 4 4 3 1 8 7
Bud 0 1 0 1 0 3 1 2 3 – 8 2 1 0 1 0 0 0 3 6 4 3 9 7
Bud Neck 0 0 13 2 1 4 7 4 3 – 0 1 0 0 0 0 0 0 2 2 0 1 4 1
Bud Site 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 4 7 5 4 1 5 2 3 – 4 2 2 3 2 41 43 18 0 0 2 0 1 1
Cytoplasm 299 582 577 340 328 349 459 391 351 – 200 164 329 296 350 523 487 318 275 555 513 106 181 137
Endoplasmic Reticulum 3 6 2 0 1 0 1 0 0 – 1 0 0 2 0 11 20 21 0 2 3 10 18 18
Endosome 2 0 0 0 0 1 2 2 0 – 0 0 0 0 0 4 3 5 4 10 3 1 18 23
Golgi 0 3 0 0 0 1 0 1 0 – 0 0 0 0 0 4 1 2 1 6 0 1 8 8
Mitochondria 0 7 1 5 5 18 50 23 106 – 97 51 0 0 1 6 4 3 0 10 21 20 52 26
Nucleus 1 2 4 1 2 0 2 3 1 – 1 2 0 2 1 2 2 1 2 0 1 0 0 0
Nuclear Periphery 0 2 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2 0
SpindlePole 1 0 2 0 0 1 1 5 1 – 0 2 0 0 1 1 2 2 4 3 4 0 5 3
Vac/Vac Membrane 0 7 1 1 2 0 3 5 0 – 0 4 0 0 2 54 39 24 2 7 5 6 21 20
Unique Cell Count 305 603 588 341 334 362 490 410 395 250 199 329 299 353 552 516 341 306 616 580 158 342 262
Labelled Cell Count 307 614 610 355 343 379 534 438 469 312 228 332 303 358 647 602 394 306 616 580 158 342 262


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.8 5.5 4.8 5.6 4.3 4.6 4.9 3.9 – 3.5 4.3 5.6 5.7 5.7 8.6 8.8 7.6 5.2 5.7 6.6
Std Deviation (1e-4) 1.1 1.0 1.3 1.4 1.2 1.3 0.9 1.5 0.8 – 0.8 0.9 1.6 1.5 1.5 1.4 1.5 1.4 1.5 1.6 2.4
Intensity Change (Log2) – – – -0.19 0.02 -0.36 -0.26 -0.16 -0.5 – -0.65 -0.36 0.02 0.05 0.05 0.65 0.69 0.46 -0.08 0.05 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 -2.3 0 0 -1.5 0 – 0 0 0 0 0 -3.5 -3.4 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 5.2 5.7 3.7
Cytoplasm 2.1 0.1 -1.6 -3.8 -2.5 -6.2 – -9.2 -8.2 2.5 1.0 1.3 -3.1 -3.3 -3.8
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.6 4.2 5.5
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 5.5 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 7.6 6.6 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4 2.3399 1.8704 1.9273 0.9786 2.0763 0.894 1.4789 1.4678 1.1165 1.9428 1.3361 1.7405 1.461 1.3768 1.2114 1.7356 1.6892
Actin 0.0163 0.0002 0.0083 0.0193 0.0002 0.0026 0.0159 0.0012 0.0017 0.0011 0.0406 0.0099 0.0185 0.004 0.0118 0.0009 0.0344 0.0393
Bud 0.0008 0.0001 0.0014 0.0004 0 0.0001 0.0021 0.0091 0.008 0.0134 0.0052 0.0014 0.0496 0.0941 0.0931 0.0547 0.1218 0.0441
Bud Neck 0.0019 0.0001 0.0003 0.0023 0.0002 0.0014 0.0045 0.0006 0.0016 0.0015 0.0047 0.0113 0.0098 0.0024 0.0024 0.0029 0.0067 0.007
Bud Periphery 0.0032 0.0001 0.0022 0.0006 0.0001 0.0002 0.0074 0.0042 0.0085 0.0078 0.0071 0.0017 0.0279 0.0261 0.0386 0.0265 0.0894 0.0299
Bud Site 0.0052 0.0004 0.0044 0.0004 0 0.0001 0.0056 0.0363 0.0146 0.0085 0.0143 0.0013 0.0401 0.1503 0.0605 0.0175 0.0235 0.0047
Cell Periphery 0.0016 0.0002 0.0003 0.0002 0.0001 0.0001 0.0005 0.0005 0.0004 0.0004 0.0007 0.0002 0.0021 0.0027 0.0024 0.0007 0.0023 0.0013
Cytoplasm 0.1618 0.4002 0.243 0.3068 0.5208 0.4133 0.26 0.4365 0.3564 0.3653 0.2894 0.4361 0.1484 0.224 0.2089 0.1779 0.165 0.274
Cytoplasmic Foci 0.0068 0.0032 0.0114 0.0093 0.002 0.0054 0.0285 0.0134 0.0171 0.0204 0.053 0.0172 0.054 0.0098 0.0189 0.0097 0.0168 0.0281
Eisosomes 0.0007 0.0001 0.0003 0.0002 0 0.0001 0.0005 0.0001 0.0001 0.0001 0.0005 0.0002 0.0006 0.0002 0.0003 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.0063 0.0018 0.0015 0.0013 0.0026 0.0015 0.0027 0.0017 0.0013 0.0022 0.0038 0.0016 0.0109 0.0017 0.004 0.0014 0.0105 0.0068
Endosome 0.0093 0.0007 0.0025 0.006 0.0006 0.0036 0.0198 0.0035 0.0076 0.0066 0.0296 0.0038 0.0241 0.0012 0.0055 0.0014 0.0498 0.0147
Golgi 0.0028 0.0001 0.0021 0.0055 0.0001 0.0008 0.0128 0.0005 0.0009 0.0003 0.0138 0.0006 0.0036 0.0003 0.0008 0.0002 0.0085 0.004
Lipid Particles 0.0091 0.0002 0.0082 0.0034 0 0.004 0.0179 0.0003 0.0033 0.0003 0.038 0.0003 0.0101 0.0005 0.0039 0.0003 0.0054 0.0023
Mitochondria 0.0136 0.0001 0.0063 0.0017 0.0001 0.0013 0.0199 0.0007 0.0052 0.0023 0.0245 0.0009 0.0193 0.002 0.0048 0.0019 0.0147 0.008
None 0.7437 0.5902 0.689 0.635 0.4726 0.5608 0.5589 0.4784 0.5472 0.5103 0.3331 0.5055 0.4801 0.378 0.4084 0.5986 0.2789 0.442
Nuclear Periphery 0.0048 0.0003 0.0007 0.0003 0.0002 0.0008 0.0053 0.0009 0.0036 0.0028 0.006 0.0009 0.0144 0.0049 0.0099 0.0061 0.0282 0.0123
Nucleolus 0.0008 0.0001 0.001 0.0001 0 0.0001 0.001 0.0007 0.0022 0.0036 0.003 0.0003 0.0082 0.0052 0.0081 0.0057 0.0088 0.003
Nucleus 0.0016 0.0007 0.0024 0.0004 0.0003 0.0008 0.002 0.0046 0.0075 0.0203 0.0061 0.0019 0.0266 0.0498 0.0551 0.0497 0.0626 0.0304
Peroxisomes 0.0033 0 0.0109 0.0052 0 0.0005 0.0285 0.0008 0.0025 0.0005 0.0849 0.0005 0.0089 0.0006 0.0013 0.0005 0.0036 0.0013
Punctate Nuclear 0.0032 0.0004 0.0028 0.0005 0.0001 0.0016 0.0025 0.0036 0.0064 0.0275 0.0349 0.0033 0.0282 0.0388 0.0539 0.0403 0.0513 0.0413
Vacuole 0.0024 0.0006 0.0007 0.0009 0.0002 0.0008 0.0024 0.0022 0.0031 0.0039 0.0055 0.0009 0.0118 0.0031 0.0062 0.0026 0.0114 0.0041
Vacuole Periphery 0.0008 0 0.0002 0.0002 0 0.0002 0.0015 0.0002 0.0008 0.0009 0.0013 0.0003 0.0027 0.0005 0.0013 0.0005 0.0063 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5608 16.2589 13.9416 27.9964 14.1489 10.9067 21.9192 28.725 22.5878 23.3146
Translational Efficiency 0.3824 0.5062 0.6505 0.3491 0.4199 0.61 0.4342 0.2931 0.4436 0.3473

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
137 1786 1387 1311 1899 1361 2160 302 2036 3147 3547 1613

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.56 757.28 920.86 853.98 689.28 711.00 861.43 859.61 689.64 737.27 884.67 855.03
Standard Deviation 84.92 103.44 119.02 123.14 82.88 96.91 108.16 137.43 83.02 103.25 116.21 125.95
Intensity Change Log 2 — 0.124727 0.406883 0.298103 — 0.044759 0.321643 0.318592 — 0.085450 0.365055 0.308345

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000253 0.000270 0.000302 0.000748 0.000099 0.000611 0.000107 0.000730 0.000109 0.000417 0.000184 0.000745
Bud Neck 0.008340 0.011347 0.003454 0.007992 0.011063 0.029604 0.008491 0.006622 0.010880 0.019243 0.006521 0.007735
Bud Site 0.002602 0.009057 0.015630 0.047420 0.005260 0.028251 0.010025 0.213742* 0.005082 0.017358 0.012217 0.078560
Cell Periphery 0.000212 0.000068 0.000133 0.000129 0.000107 0.000336 0.000102 0.000175 0.000114 0.000184 0.000114 0.000138
Cytoplasm 0.524347* 0.620223* 0.500000* 0.589463* 0.577067* 0.484713* 0.582645* 0.522326* 0.573519* 0.561618* 0.550328* 0.576893*
Cytoplasmic Foci 0.259677* 0.160266 0.014908 0.022349 0.188963* 0.241352* 0.010813 0.034898 0.193721* 0.195334* 0.012414 0.024698
Eisosomes 0.000277 0.000054 0.000033 0.000032 0.000120 0.000132 0.000027 0.000025 0.000130 0.000087 0.000029 0.000031
Endoplasmic Reticulum 0.000407 0.000736 0.003254 0.001222 0.000658 0.000630 0.000999 0.000410 0.000641 0.000690 0.001881 0.001070
Endosome 0.011673 0.011871 0.002797 0.005631 0.009437 0.028499 0.002038 0.015215 0.009588 0.019062 0.002335 0.007425
Golgi 0.004351 0.001537 0.000090 0.006189 0.002080 0.009316 0.000095 0.000666 0.002232 0.004901 0.000093 0.005155
Lipid Particles 0.008846 0.002536 0.001482 0.000946 0.005212 0.005518 0.000726 0.000652 0.005456 0.003826 0.001022 0.000891
Mitochondria 0.001932 0.001749 0.000485 0.003012 0.002335 0.009080 0.000926 0.000640 0.002308 0.004919 0.000753 0.002568
Mitotic Spindle 0.001497 0.004278 0.005348 0.045689 0.000683 0.007284 0.015608 0.033001 0.000738 0.005578 0.011596 0.043314
None 0.002195 0.003361 0.005272 0.006000 0.003047 0.002097 0.005131 0.003769 0.002990 0.002814 0.005186 0.005583
Nuclear Periphery 0.000262 0.000246 0.001802 0.001013 0.000322 0.000230 0.001359 0.000285 0.000318 0.000239 0.001532 0.000876
Nuclear Periphery Foci 0.000517 0.000517 0.002007 0.001485 0.000316 0.000357 0.000645 0.000864 0.000329 0.000448 0.001178 0.001369
Nucleolus 0.000866 0.001116 0.000364 0.000366 0.000919 0.001727 0.000453 0.000455 0.000916 0.001380 0.000418 0.000383
Nucleus 0.109263 0.085881 0.363971* 0.128098 0.135328* 0.044570 0.293484* 0.027665 0.133574* 0.068015 0.321047* 0.109294
Peroxisomes 0.003124 0.003629 0.000303 0.000835 0.002263 0.011145 0.000207 0.004544 0.002321 0.006880 0.000245 0.001530
Vacuole 0.058508 0.080112 0.078151 0.128108 0.053872 0.091246 0.065741 0.132606 0.054184 0.084927 0.070594 0.128950
Vacuole Periphery 0.000852 0.001147 0.000212 0.003272 0.000849 0.003301 0.000377 0.000709 0.000849 0.002078 0.000313 0.002792

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.20 -0.90 -1.07 -1.41 -0.55 -2.71 -2.55 -4.77 0.03 -4.40 -3.49 -6.50 -4.50 -0.41 -2.94
Bud Neck -1.30 1.75 0.90 4.64 -2.12 -8.93 3.53 3.38 10.49 0.35 -6.87 5.49 4.68 11.03 -0.20
Bud Site -5.81 -4.46 -7.10 -4.82 -3.22 -11.23 -1.91 -8.38 -6.67 -8.21 -10.72 -3.60 -10.05 -6.49 -8.74
Cell Periphery 5.54 2.75 4.60 -3.89 5.99 -1.15 2.70 -0.91 1.07 -1.97 -0.80 0.82 2.52 0.98 1.82
Cytoplasm -4.75 5.61 2.11 13.50 -6.39 9.54 10.92 6.86 2.12 2.18 1.69 15.96 10.74 9.70 -2.06
Cytoplasmic Foci 6.17 15.81 15.51 32.44 -3.56 -7.37 41.68 27.35 30.89 -5.42 -0.19 43.87 40.54 47.78 -7.87
Eisosomes 5.82 6.04 6.26 4.89 3.72 -1.20 12.76 12.02 12.16 -0.17 5.56 13.46 13.70 12.82 1.23
Endoplasmic Reticulum -1.60 -16.75 -8.21 -4.55 12.49 0.31 -12.04 -1.46 -1.53 8.00 -0.38 -19.60 -8.50 -5.97 11.43
Endosome -0.10 4.33 4.32 10.23 0.27 -11.50 12.57 -0.48 6.15 -3.67 -9.46 12.52 6.44 14.83 -3.74
Golgi 1.03 1.56 0.84 -0.44 -2.07 -6.38 7.80 5.84 7.99 -4.15 -4.64 7.13 0.74 3.72 -2.19
Lipid Particles 2.63 2.98 3.23 4.97 4.00 -0.48 9.45 8.23 7.05 -2.00 2.97 9.27 9.41 8.79 0.72
Mitochondria 0.18 1.38 0.53 0.79 -2.12 -5.48 4.56 5.93 7.27 3.79 -4.17 5.18 2.61 5.95 -1.43
Mitotic Spindle -1.58 -2.50 -8.03 -7.37 -6.96 -3.93 -7.17 -5.03 -4.08 -3.44 -4.79 -8.27 -10.28 -8.25 -6.89
None -1.19 -2.46 -3.07 -2.79 -1.45 1.72 -4.33 0.20 -1.19 3.40 0.38 -4.48 -3.39 -3.85 -0.07
Nuclear Periphery 0.38 -22.38 -8.71 -8.96 3.28 1.76 -21.20 -5.53 -6.58 10.55 1.85 -28.03 -8.98 -9.77 4.45
Nuclear Periphery Foci -0.14 -9.94 -5.92 -6.62 4.55 -0.77 -7.49 -3.10 -3.03 -2.21 -2.20 -13.89 -8.14 -7.26 -0.87
Nucleolus -0.74 1.31 0.97 4.19 -1.33 -1.46 2.22 1.69 2.81 -0.95 -1.22 2.41 1.97 4.35 -1.30
Nucleus 2.01 -20.47 -4.75 -14.97 24.85 22.52 -31.85 15.13 -0.22 40.29 17.48 -41.09 -2.36 -16.94 34.99
Peroxisomes -0.58 2.85 2.67 9.00 -1.91 -8.82 10.62 -1.26 5.51 -3.47 -8.89 10.80 4.80 11.47 -4.14
Vacuole -3.58 -9.97 -19.95 -21.01 -11.98 -10.04 -14.97 -13.13 -10.90 -9.28 -11.49 -19.74 -28.19 -23.53 -16.98
Vacuole Periphery -0.56 1.35 -0.37 0.14 -2.40 -6.88 6.99 3.60 7.86 -2.59 -6.93 8.07 -0.38 3.20 -2.48
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YGL036W

YGL036W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygl036w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available