ORF
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.98 1.0 0.98 0.96 0.94 0.95 0.89 0.8 0.82 1.0 0.99 0.99 0.95 0.94 0.93 0.9 0.9 0.89 0.68 0.53 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.07 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.1 0.06 0.27 0.39 0.26 0 0 0 0 0 0 0 0 0 0.13 0.15 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.07 0 0 0 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 2 0 1 0 0 0 0 0 1 1 0 4 4 3 1 8 7
Bud 0 1 0 1 0 3 1 2 3 8 2 1 0 1 0 0 0 3 6 4 3 9 7
Bud Neck 0 0 13 2 1 4 7 4 3 0 1 0 0 0 0 0 0 2 2 0 1 4 1
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 4 7 5 4 1 5 2 3 4 2 2 3 2 41 43 18 0 0 2 0 1 1
Cytoplasm 299 582 577 340 328 349 459 391 351 200 164 329 296 350 523 487 318 275 555 513 106 181 137
Endoplasmic Reticulum 3 6 2 0 1 0 1 0 0 1 0 0 2 0 11 20 21 0 2 3 10 18 18
Endosome 2 0 0 0 0 1 2 2 0 0 0 0 0 0 4 3 5 4 10 3 1 18 23
Golgi 0 3 0 0 0 1 0 1 0 0 0 0 0 0 4 1 2 1 6 0 1 8 8
Mitochondria 0 7 1 5 5 18 50 23 106 97 51 0 0 1 6 4 3 0 10 21 20 52 26
Nucleus 1 2 4 1 2 0 2 3 1 1 2 0 2 1 2 2 1 2 0 1 0 0 0
Nuclear Periphery 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
SpindlePole 1 0 2 0 0 1 1 5 1 0 2 0 0 1 1 2 2 4 3 4 0 5 3
Vac/Vac Membrane 0 7 1 1 2 0 3 5 0 0 4 0 0 2 54 39 24 2 7 5 6 21 20
Unique Cell Count 305 603 588 341 334 362 490 410 395 250 199 329 299 353 552 516 341 306 616 580 158 342 262
Labelled Cell Count 307 614 610 355 343 379 534 438 469 312 228 332 303 358 647 602 394 306 616 580 158 342 262


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.8 5.5 4.8 5.6 4.3 4.6 4.9 3.9 3.5 4.3 5.6 5.7 5.7 8.6 8.8 7.6 5.2 5.7 6.6
Std Deviation (1e-4) 1.1 1.0 1.3 1.4 1.2 1.3 0.9 1.5 0.8 0.8 0.9 1.6 1.5 1.5 1.4 1.5 1.4 1.5 1.6 2.4
Intensity Change (Log2) -0.19 0.02 -0.36 -0.26 -0.16 -0.5 -0.65 -0.36 0.02 0.05 0.05 0.65 0.69 0.46 -0.08 0.05 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 -2.3 0 0 -1.5 0 0 0 0 0 0 -3.5 -3.4 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 5.2 5.7 3.7
Cytoplasm 2.1 0.1 -1.6 -3.8 -2.5 -6.2 -9.2 -8.2 2.5 1.0 1.3 -3.1 -3.3 -3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.6 4.2 5.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 5.5 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 7.6 6.6 6.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygl036w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available