Standard name
Description Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.06 0 0.07 0.05 0.06 0 0 0 0 0 0 0.15 0.18 0.31 0.23 0.2 0.15 0 0 0 0.08 0.12 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.08 0.28 0.2 0.2 0.5 0.58 0.49 0.57 0.66 0.67 0.68 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Nucleus 0.75 0.77 0.77 0.8 0.76 0.66 0.59 0.69 0.7 0.65 0.66 0.64 0.78 0.64 0.44 0.59 0.52 0.66 0.71 0.75 0.68 0.6 0.38 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0.07 0 0.09 0.08 0.1 0.11 0 0 0 0 0 0 0.1 0.06 0.06 0.05 0.12 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08
Vac/Vac Membrane 0.11 0.15 0.08 0 0.09 0.11 0 0 0 0 0 0.05 0.06 0.16 0.27 0.2 0.26 0.18 0.05 0.05 0.12 0.13 0.11 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud 0 0 1 0 0 0 5 0 0 3 0 3 0 0 0 1 0 0 4 5 5 5 18 19
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 6
Bud Site 0 1 0 0 1 3 4 5 8 18 4 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 11 4 3 8 14 13 16 8 14 6 13 23 45 65 69 27 13 4 4 3 8 31 33
Endoplasmic Reticulum 0 1 0 0 0 1 0 0 1 0 0 0 0 1 1 13 12 5 0 0 0 0 1 1
Endosome 0 2 0 0 0 0 0 0 1 0 0 0 0 7 7 11 8 2 4 5 1 2 12 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 1 0 0 0 2
Mitochondria 36 15 36 9 30 118 189 185 158 209 145 199 3 5 1 14 1 5 0 2 0 0 2 3
Nucleus 105 143 99 35 113 157 193 259 196 206 142 187 119 155 94 179 71 59 107 165 103 62 100 56
Nuclear Periphery 0 0 0 0 1 0 4 3 2 7 2 0 0 0 0 0 0 1 0 0 0 0 1 1
Nucleolus 8 8 2 0 4 1 22 16 25 24 21 32 2 5 4 3 2 0 14 14 9 4 32 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 0 3 0 0 0 1 0 1 0 0 3 4 3 4 26 17
Vac/Vac Membrane 16 27 10 1 13 25 10 12 5 5 9 14 9 38 57 60 36 16 7 11 18 13 30 32
Unique Cell Count 140 185 128 44 149 238 325 377 279 316 215 291 152 244 212 304 137 89 151 219 152 104 267 214
Labelled Cell Count 173 208 152 48 170 320 441 497 404 489 329 454 156 259 229 354 158 102 151 219 152 104 267 214


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.2 4.9 5.1 5.1 4.7 4.1 4.6 4.6 4.2 4.3 4.3 6.9 6.4 6.0 7.8 9.3 10.9 6.4 6.7 7.3
Std Deviation (1e-4) 0.9 1.1 0.9 1.3 1.1 1.2 1.0 1.0 1.1 1.2 1.0 1.1 1.4 1.3 1.2 1.9 2.1 2.2 1.6 1.8 1.8
Intensity Change (Log2) – – – 0.07 0.06 -0.07 -0.25 -0.09 -0.1 -0.22 -0.21 -0.19 0.49 0.37 0.3 0.67 0.92 1.15 0.38 0.45 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.9 1.2 0 0.6 0 0.6 0 0.6 3.4 4.2 6.1 5.0 4.2 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 -1.6 0 0 0 0 0 0 0 -6.3 -7.6 -7.9 -7.0 -6.4 -4.2
Nucleus 0.3 -0.3 -2.3 -3.6 -1.9 -1.5 -2.5 -2.2 -2.6 0.2 -2.7 -6.0 -3.7 -4.3 -1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.3 0.8 -2.2 -2.2 0 0 -1.4 -1.2 -0.6 2.1 4.3 3.1 4.0 2.3