Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 1 | 10 | 27 | 28 | 26 | 30 | 49 | – | 41 | 31 | 35 | 14 | 32 | 34 | 14 | 11 | 8 | 0 | 0 | 0 | 1 | 2 | 5 |
Bud | – | 0 | 0 | 2 | 2 | 5 | 8 | 10 | – | 6 | 7 | 6 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 2 | 13 | 20 | 34 | 24 | 36 | 29 | – | 51 | 36 | 51 | 31 | 91 | 141 | 72 | 46 | 47 | 0 | 1 | 2 | 0 | 3 | 5 |
Cytoplasm | – | 6 | 9 | 21 | 23 | 65 | 39 | 50 | – | 53 | 35 | 69 | 24 | 37 | 29 | 36 | 23 | 27 | 1 | 3 | 0 | 1 | 11 | 15 |
Endoplasmic Reticulum | – | 63 | 132 | 255 | 287 | 382 | 527 | 258 | – | 401 | 219 | 256 | 202 | 508 | 500 | 1 | 2 | 0 | 17 | 91 | 138 | 143 | 281 | 347 |
Endosome | – | 0 | 0 | 0 | 1 | 0 | 0 | 4 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Golgi | – | 0 | 3 | 12 | 3 | 1 | 2 | 3 | – | 3 | 0 | 0 | 1 | 11 | 12 | 19 | 23 | 10 | 0 | 0 | 0 | 1 | 1 | 1 |
Mitochondria | – | 0 | 5 | 22 | 45 | 85 | 122 | 90 | – | 88 | 142 | 203 | 0 | 1 | 0 | 6 | 10 | 10 | 0 | 1 | 0 | 2 | 3 | 4 |
Nucleus | – | 1 | 0 | 0 | 1 | 0 | 1 | 1 | – | 3 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 1 | 6 | 9 | 4 | 8 | 46 | 14 | – | 28 | 25 | 25 | 0 | 4 | 5 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 1 | 0 | 4 | 10 | 36 | 57 | 177 | – | 79 | 20 | 57 | 26 | 6 | 25 | 1 | 8 | 3 | 0 | 0 | 1 | 2 | 10 | 8 |
Unique Cell Count | 70 | 141 | 286 | 340 | 502 | 666 | 557 | 585 | 369 | 525 | 259 | 580 | 603 | 140 | 111 | 94 | 21 | 102 | 145 | 158 | 317 | 397 | ||
Labelled Cell Count | 75 | 178 | 372 | 438 | 632 | 868 | 689 | 755 | 516 | 703 | 299 | 692 | 748 | 151 | 127 | 106 | 21 | 102 | 145 | 158 | 317 | 397 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 18.9 | 14.3 | 14.3 | 12.4 | 10.8 | 10.0 | 9.9 | – | 9.7 | 8.9 | 8.8 | 15.5 | 16.1 | 15.4 | 17.9 | 18.3 | 18.6 | 17.2 | 17.2 | 17.1 |
Std Deviation (1e-4) | – | 3.1 | 2.1 | 2.2 | 2.0 | 1.7 | 1.7 | 1.6 | – | 1.7 | 1.7 | 1.6 | 2.5 | 2.5 | 2.6 | 3.5 | 3.3 | 3.8 | 2.6 | 2.7 | 2.6 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.21 | -0.42 | -0.52 | -0.53 | – | -0.56 | -0.68 | -0.71 | 0.11 | 0.16 | 0.11 | 0.32 | 0.35 | 0.38 | 0.26 | 0.26 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.8 | 0.4 | -0.9 | -1.3 | 0.7 | – | 0 | 0.5 | -0.2 | -0.7 | -0.7 | -0.7 | 0.9 | 0.8 | 0.4 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.8 | 0.3 | -2.0 | -1.7 | -1.8 | – | -0.2 | 0.2 | 0.2 | 0.8 | 2.0 | 3.7 | 7.7 | 6.0 | 7.0 |
Cytoplasm | 0.4 | 0.2 | 2.2 | -0.2 | 1.0 | – | 1.0 | 1.1 | 2.2 | 1.0 | 0 | -0.8 | 4.4 | 3.4 | 4.7 |
Endoplasmic Reticulum | -1.5 | -2.7 | -4.6 | -4.0 | -10.1 | – | -6.0 | -7.5 | -9.6 | -4.0 | -2.0 | -3.2 | -15.6 | -14.5 | -14.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 4.8 | 2.8 |
Mitochondria | 1.7 | 3.2 | 4.0 | 4.4 | 3.9 | – | 3.7 | 7.8 | 8.0 | 0 | 0 | 0 | 0.3 | 1.8 | 2.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1.2 | 0 | – | 0.3 | 1.1 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.3 | 3.6 | 7.7 | – | 4.6 | 2.8 | 4.1 | 3.9 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.1775 | 14.1982 | 12.2814 | 10.9139 | 10.3457 | 12.6465 | 12.6491 | 14.0763 | 12.7087 | 12.8378 | 11.2286 | 12.652 | 13.1255 | 13.086 | 12.1693 | 12.8858 | 11.0972 | 12.5187 |
Actin | 0.0335 | 0.0045 | 0.0243 | 0.0406 | 0.0079 | 0.0008 | 0.0327 | 0.0001 | 0.0014 | 0.0007 | 0.0118 | 0.0012 | 0.0226 | 0.0002 | 0.0142 | 0.0001 | 0.0179 | 0.0012 |
Bud | 0.0012 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0029 | 0.001 | 0.0006 | 0.0214 | 0.0114 | 0.0011 | 0.0006 | 0.0001 | 0.0003 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0016 | 0.0005 | 0.001 | 0.0004 |
Bud Periphery | 0.0022 | 0.0002 | 0.0003 | 0.001 | 0.0007 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.011 | 0.0028 | 0.002 | 0.0087 | 0.0009 | 0 | 0.0018 | 0.0001 | 0.0005 | 0 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0 | 0.0006 | 0 |
Cell Periphery | 0.0034 | 0.009 | 0.0073 | 0.0012 | 0.0016 | 0.0021 | 0.0015 | 0.0037 | 0.0041 | 0.0012 | 0.0017 | 0.0009 | 0.0009 | 0.0053 | 0.0025 | 0.0015 | 0.0026 | 0.0014 |
Cytoplasm | 0.0168 | 0.0387 | 0.0072 | 0.0014 | 0.0133 | 0.0108 | 0.0179 | 0.0083 | 0.0082 | 0.0251 | 0.0077 | 0.0052 | 0.0112 | 0.0095 | 0.0043 | 0.0013 | 0.0136 | 0.0048 |
Cytoplasmic Foci | 0.0222 | 0.0062 | 0.0043 | 0.0232 | 0.0375 | 0.0006 | 0.0102 | 0.0002 | 0.0071 | 0.0008 | 0.0106 | 0.0016 | 0.0112 | 0.0001 | 0.0046 | 0 | 0.0122 | 0.0006 |
Eisosomes | 0.0005 | 0.0002 | 0.0003 | 0.0011 | 0.0005 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.7347 | 0.8876 | 0.8968 | 0.7059 | 0.7296 | 0.9388 | 0.8463 | 0.9631 | 0.9449 | 0.948 | 0.8012 | 0.9673 | 0.9021 | 0.9745 | 0.927 | 0.9893 | 0.8718 | 0.9742 |
Endosome | 0.024 | 0.0051 | 0.0109 | 0.0164 | 0.0401 | 0.0047 | 0.0155 | 0.0003 | 0.0075 | 0.0045 | 0.048 | 0.0059 | 0.0055 | 0.0003 | 0.0041 | 0.0005 | 0.0109 | 0.007 |
Golgi | 0.0074 | 0.0035 | 0.0092 | 0.0213 | 0.0137 | 0.0049 | 0.0078 | 0.0002 | 0.0057 | 0.004 | 0.0153 | 0.0019 | 0.0035 | 0.0004 | 0.0067 | 0.0001 | 0.0061 | 0.0009 |
Lipid Particles | 0.0236 | 0.0192 | 0.0059 | 0.0363 | 0.0134 | 0.0023 | 0.0113 | 0.0204 | 0.0036 | 0.0099 | 0.0301 | 0.0043 | 0.0073 | 0.0037 | 0.0097 | 0.0001 | 0.0146 | 0.0008 |
Mitochondria | 0.0177 | 0.0004 | 0.0033 | 0.0521 | 0.0982 | 0.0043 | 0.0072 | 0 | 0.0046 | 0.0002 | 0.0273 | 0.0022 | 0.0065 | 0 | 0.0053 | 0.0001 | 0.0018 | 0.0003 |
None | 0.0034 | 0.0006 | 0.0087 | 0.0053 | 0.0013 | 0.0002 | 0.0101 | 0 | 0.0004 | 0.0001 | 0.0065 | 0.0003 | 0.0007 | 0 | 0.0019 | 0 | 0.0009 | 0.0003 |
Nuclear Periphery | 0.0457 | 0.01 | 0.0071 | 0.0118 | 0.0065 | 0.0193 | 0.0177 | 0.0021 | 0.0032 | 0.0029 | 0.0166 | 0.0041 | 0.02 | 0.0039 | 0.0047 | 0.0046 | 0.0193 | 0.0053 |
Nucleolus | 0.0013 | 0.0001 | 0.0011 | 0.0232 | 0.0005 | 0.0001 | 0.0007 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Nucleus | 0.007 | 0.0015 | 0.0021 | 0.0029 | 0.0012 | 0.004 | 0.0076 | 0.0001 | 0.0002 | 0.0002 | 0.0016 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0008 | 0.0005 |
Peroxisomes | 0.0099 | 0.0036 | 0.0026 | 0.0168 | 0.004 | 0.0001 | 0.0031 | 0 | 0.0025 | 0.0001 | 0.0053 | 0.0017 | 0.0032 | 0 | 0.0084 | 0 | 0.0119 | 0.0001 |
Punctate Nuclear | 0.0152 | 0.0006 | 0.0024 | 0.0027 | 0.0134 | 0.0001 | 0.0022 | 0 | 0.0017 | 0.0002 | 0.0016 | 0.0009 | 0.0005 | 0 | 0.0009 | 0 | 0.0066 | 0.0003 |
Vacuole | 0.0128 | 0.0049 | 0.0033 | 0.0029 | 0.0023 | 0.0036 | 0.0026 | 0.0011 | 0.003 | 0.001 | 0.0085 | 0.0014 | 0.002 | 0.0016 | 0.0018 | 0.0013 | 0.0059 | 0.0012 |
Vacuole Periphery | 0.0035 | 0.0002 | 0.0004 | 0.0031 | 0.0018 | 0.0015 | 0.0013 | 0.0001 | 0.0005 | 0.0002 | 0.0031 | 0.0005 | 0.0007 | 0.0001 | 0.0005 | 0.0003 | 0.001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.9489 | 44.582 | 32.0479 | 33.3237 | 36.3715 | 35.8252 | 35.9206 | 36.6036 | 36.9729 | 45.2397 |
Translational Efficiency | 2.5205 | 1.3736 | 1.6323 | 1.2978 | 1.6637 | 1.9671 | 1.6846 | 1.4461 | 1.5943 | 1.5155 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1570 | 1538 | 1330 | 1366 | 1424 | 1546 | 1185 | 1266 | 2994 | 3084 | 2515 | 2632 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1123.18 | 1448.38 | 1736.71 | 1747.55 | 1230.72 | 1368.89 | 1614.66 | 1748.58 | 1174.33 | 1408.53 | 1679.20 | 1748.05 |
Standard Deviation | 211.72 | 259.20 | 263.76 | 310.02 | 233.19 | 228.23 | 377.66 | 291.77 | 228.59 | 247.38 | 328.18 | 301.38 |
Intensity Change Log 2 | — | 0.366851 | 0.628768 | 0.637745 | — | 0.153504 | 0.391728 | 0.506681 | — | 0.259245 | 0.509698 | 0.570706 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001191 | 0.000799 | 0.000961 | 0.002365 | 0.000956 | 0.001126 | 0.001050 | 0.000575 | 0.001079 | 0.000963 | 0.001003 | 0.001504 |
Bud Neck | 0.000332 | 0.000373 | 0.002102 | 0.002283 | 0.000273 | 0.000439 | 0.001355 | 0.000951 | 0.000304 | 0.000406 | 0.001750 | 0.001642 |
Bud Site | 0.005910 | 0.003418 | 0.007146 | 0.015951 | 0.004957 | 0.003023 | 0.014067 | 0.004453 | 0.005457 | 0.003220 | 0.010407 | 0.010420 |
Cell Periphery | 0.004936 | 0.005918 | 0.011695 | 0.044550 | 0.007058 | 0.005403 | 0.026107 | 0.043196 | 0.005945 | 0.005660 | 0.018486 | 0.043899 |
Cytoplasm | 0.005585 | 0.002169 | 0.003284 | 0.009934 | 0.006993 | 0.001856 | 0.017901 | 0.003819 | 0.006255 | 0.002012 | 0.010171 | 0.006993 |
Cytoplasmic Foci | 0.008678 | 0.001624 | 0.000401 | 0.005529 | 0.009101 | 0.001132 | 0.007135 | 0.001301 | 0.008880 | 0.001377 | 0.003574 | 0.003495 |
Eisosomes | 0.000100 | 0.000116 | 0.000135 | 0.000326 | 0.000101 | 0.000100 | 0.000256 | 0.000267 | 0.000100 | 0.000108 | 0.000192 | 0.000297 |
Endoplasmic Reticulum | 0.949714* | 0.972597* | 0.950651* | 0.819500* | 0.943976* | 0.973137* | 0.850050* | 0.889573* | 0.946985* | 0.972868* | 0.903251* | 0.853205* |
Endosome | 0.007034 | 0.002888 | 0.004699 | 0.011929 | 0.008495 | 0.004186 | 0.011026 | 0.005845 | 0.007729 | 0.003539 | 0.007680 | 0.009002 |
Golgi | 0.003458 | 0.001450 | 0.001268 | 0.012804 | 0.004327 | 0.001281 | 0.014368 | 0.007204 | 0.003872 | 0.001365 | 0.007440 | 0.010111 |
Lipid Particles | 0.003207 | 0.003639 | 0.007334 | 0.043043 | 0.003733 | 0.002299 | 0.017979 | 0.022732 | 0.003457 | 0.002967 | 0.012350 | 0.033273 |
Mitochondria | 0.001869 | 0.000503 | 0.000173 | 0.001011 | 0.001073 | 0.000551 | 0.001212 | 0.000579 | 0.001490 | 0.000527 | 0.000663 | 0.000804 |
Mitotic Spindle | 0.000090 | 0.000179 | 0.000494 | 0.000744 | 0.000134 | 0.000028 | 0.000497 | 0.000520 | 0.000111 | 0.000103 | 0.000495 | 0.000636 |
None | 0.001362 | 0.001089 | 0.000094 | 0.000705 | 0.000933 | 0.000884 | 0.001363 | 0.000489 | 0.001158 | 0.000987 | 0.000692 | 0.000601 |
Nuclear Periphery | 0.000181 | 0.000135 | 0.000575 | 0.001399 | 0.000220 | 0.000143 | 0.001133 | 0.001276 | 0.000200 | 0.000139 | 0.000838 | 0.001340 |
Nuclear Periphery Foci | 0.001525 | 0.000522 | 0.002911 | 0.005679 | 0.002629 | 0.000830 | 0.008841 | 0.001828 | 0.002050 | 0.000676 | 0.005705 | 0.003826 |
Nucleolus | 0.000013 | 0.000023 | 0.000006 | 0.000047 | 0.000014 | 0.000016 | 0.000035 | 0.000031 | 0.000014 | 0.000020 | 0.000020 | 0.000039 |
Nucleus | 0.000170 | 0.000105 | 0.000206 | 0.000659 | 0.000154 | 0.000160 | 0.000463 | 0.000204 | 0.000162 | 0.000133 | 0.000327 | 0.000440 |
Peroxisomes | 0.001124 | 0.000469 | 0.000090 | 0.000774 | 0.000586 | 0.000620 | 0.001341 | 0.000851 | 0.000868 | 0.000545 | 0.000679 | 0.000811 |
Vacuole | 0.002079 | 0.001601 | 0.005374 | 0.017810 | 0.002539 | 0.002063 | 0.018137 | 0.011646 | 0.002298 | 0.001833 | 0.011388 | 0.014845 |
Vacuole Periphery | 0.001440 | 0.000384 | 0.000401 | 0.002959 | 0.001748 | 0.000722 | 0.005683 | 0.002661 | 0.001587 | 0.000553 | 0.002889 | 0.002816 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.97 | 0.37 | -2.55 | -3.38 | -3.07 | -0.26 | -0.34 | 1.30 | 0.87 | 1.79 | 0.30 | 0.06 | -1.75 | -1.63 | -1.96 |
Bud Neck | -0.32 | -3.58 | -5.13 | -4.83 | -0.33 | -1.21 | -4.81 | -4.02 | -2.38 | 1.49 | -1.06 | -5.11 | -6.23 | -5.37 | 0.27 |
Bud Site | 1.84 | -1.10 | -4.75 | -5.87 | -3.48 | 1.54 | -4.60 | 0.26 | -1.05 | 4.45 | 2.41 | -4.05 | -4.01 | -5.56 | -0.01 |
Cell Periphery | -0.82 | -4.84 | -13.21 | -12.62 | -10.39 | 1.17 | -7.24 | -10.88 | -11.69 | -4.37 | 0.30 | -8.65 | -17.02 | -17.21 | -10.17 |
Cytoplasm | 3.99 | 1.99 | -3.51 | -6.71 | -5.10 | 4.95 | -4.76 | 2.19 | -2.36 | 6.05 | 6.36 | -3.39 | -1.26 | -6.85 | 2.30 |
Cytoplasmic Foci | 5.95 | 7.23 | 2.10 | -4.27 | -5.90 | 5.86 | 1.17 | 5.59 | -0.33 | 5.63 | 8.37 | 5.37 | 5.29 | -3.97 | 0.06 |
Eisosomes | -0.45 | -1.06 | -3.87 | -3.53 | -3.24 | 0.03 | -2.86 | -3.04 | -3.11 | -0.17 | -0.31 | -2.95 | -4.92 | -4.72 | -2.37 |
Endoplasmic Reticulum | -4.90 | 0.20 | 16.23 | 19.81 | 15.85 | -5.95 | 10.62 | 7.65 | 12.67 | -4.07 | -7.66 | 8.77 | 17.30 | 23.20 | 7.58 |
Endosome | 6.58 | 2.98 | -5.09 | -10.99 | -7.87 | 4.69 | -2.26 | 2.91 | -2.11 | 4.89 | 7.56 | -0.08 | -2.10 | -9.50 | -1.94 |
Golgi | 2.99 | 3.38 | -5.97 | -7.81 | -7.99 | 4.32 | -5.64 | -2.25 | -5.31 | 3.63 | 5.15 | -3.92 | -6.12 | -9.43 | -2.20 |
Lipid Particles | -0.51 | -3.70 | -12.98 | -12.61 | -11.08 | 2.20 | -6.73 | -8.64 | -9.39 | -1.54 | 0.88 | -7.64 | -15.51 | -15.63 | -9.46 |
Mitochondria | 3.17 | 4.16 | 1.73 | -1.97 | -3.64 | 1.69 | -0.23 | 1.87 | -0.11 | 1.12 | 3.56 | 2.34 | 2.41 | -1.58 | -0.54 |
Mitotic Spindle | -0.72 | -1.33 | -4.70 | -3.23 | -0.83 | 3.31 | -1.84 | -1.40 | -1.79 | -0.04 | 0.07 | -2.04 | -3.68 | -3.46 | -0.65 |
None | 0.33 | 1.91 | 0.93 | 0.69 | -3.37 | 0.12 | -0.50 | 1.12 | 1.07 | 1.05 | 0.36 | 0.87 | 1.35 | 1.17 | 0.21 |
Nuclear Periphery | 1.64 | -4.71 | -12.04 | -12.85 | -6.48 | 2.60 | -6.66 | -10.38 | -11.41 | -0.84 | 2.95 | -8.14 | -15.89 | -17.18 | -4.85 |
Nuclear Periphery Foci | 3.16 | -2.54 | -4.89 | -6.28 | -3.03 | 4.01 | -4.81 | 1.28 | -2.64 | 5.34 | 5.05 | -5.41 | -3.73 | -6.77 | 2.19 |
Nucleolus | -0.65 | 2.72 | -1.66 | -0.94 | -2.00 | -0.30 | -2.56 | -1.15 | -0.92 | 0.37 | -0.72 | -1.57 | -2.02 | -1.28 | -1.46 |
Nucleus | 1.31 | -1.16 | -3.39 | -3.77 | -2.95 | -0.07 | -1.94 | -1.44 | -0.50 | 1.75 | 0.51 | -2.13 | -3.62 | -3.46 | 0.03 |
Peroxisomes | 2.50 | 4.16 | 1.27 | -1.93 | -5.16 | -0.18 | -1.94 | -0.79 | -0.70 | 1.04 | 1.97 | 0.85 | 0.28 | -1.48 | -0.56 |
Vacuole | 0.74 | -4.28 | -9.70 | -10.01 | -7.29 | 0.56 | -8.06 | -6.97 | -7.15 | 3.00 | 0.87 | -9.04 | -11.92 | -12.26 | -2.50 |
Vacuole Periphery | 4.53 | 4.63 | -3.61 | -6.72 | -6.76 | 2.32 | -4.50 | -1.33 | -2.91 | 3.00 | 4.19 | -3.05 | -3.12 | -5.99 | 0.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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