Standard name
Description Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0.07 0.09 0.08 0.05 0.05 0.09 – 0.07 0.08 0.07 0.05 0.06 0.06 0.1 0.1 0.09 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0.09 0.07 0.1 0.05 0.05 0.05 – 0.09 0.1 0.1 0.12 0.16 0.23 0.51 0.41 0.5 0 0 0 0 0 0
Cytoplasm – 0.09 0.06 0.07 0.07 0.13 0.06 0.09 – 0.09 0.09 0.13 0.09 0.06 0.05 0.26 0.21 0.29 0.06 0 0 0 0 0
Endoplasmic Reticulum – 0.9 0.94 0.89 0.84 0.76 0.79 0.46 – 0.69 0.59 0.49 0.78 0.88 0.83 0 0 0 0.86 0.9 0.96 0.91 0.89 0.87
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.14 0.21 0.11 0 0 0 0 0 0
Mitochondria – 0 0 0.08 0.13 0.17 0.18 0.16 – 0.15 0.38 0.39 0 0 0 0 0.09 0.11 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0.07 0 – 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0.07 0.09 0.32 – 0.14 0.05 0.11 0.1 0 0 0 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 1 10 27 28 26 30 49 – 41 31 35 14 32 34 14 11 8 0 0 0 1 2 5
Bud – 0 0 2 2 5 8 10 – 6 7 6 1 0 1 0 1 0 0 0 0 0 0 2
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0
Bud Site – 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 2 13 20 34 24 36 29 – 51 36 51 31 91 141 72 46 47 0 1 2 0 3 5
Cytoplasm – 6 9 21 23 65 39 50 – 53 35 69 24 37 29 36 23 27 1 3 0 1 11 15
Endoplasmic Reticulum – 63 132 255 287 382 527 258 – 401 219 256 202 508 500 1 2 0 17 91 138 143 281 347
Endosome – 0 0 0 1 0 0 4 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Golgi – 0 3 12 3 1 2 3 – 3 0 0 1 11 12 19 23 10 0 0 0 1 1 1
Mitochondria – 0 5 22 45 85 122 90 – 88 142 203 0 1 0 6 10 10 0 1 0 2 3 4
Nucleus – 1 0 0 1 0 1 1 – 3 0 0 0 2 1 1 1 0 0 0 0 0 0 0
Nuclear Periphery – 1 6 9 4 8 46 14 – 28 25 25 0 4 5 1 2 1 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 3 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 1 0 4 10 36 57 177 – 79 20 57 26 6 25 1 8 3 0 0 1 2 10 8
Unique Cell Count 70 141 286 340 502 666 557 585 369 525 259 580 603 140 111 94 21 102 145 158 317 397
Labelled Cell Count 75 178 372 438 632 868 689 755 516 703 299 692 748 151 127 106 21 102 145 158 317 397


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 18.9 14.3 14.3 12.4 10.8 10.0 9.9 – 9.7 8.9 8.8 15.5 16.1 15.4 17.9 18.3 18.6 17.2 17.2 17.1
Std Deviation (1e-4) – 3.1 2.1 2.2 2.0 1.7 1.7 1.6 – 1.7 1.7 1.6 2.5 2.5 2.6 3.5 3.3 3.8 2.6 2.7 2.6
Intensity Change (Log2) – – – 0.0 -0.21 -0.42 -0.52 -0.53 – -0.56 -0.68 -0.71 0.11 0.16 0.11 0.32 0.35 0.38 0.26 0.26 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.8 0.4 -0.9 -1.3 0.7 – 0 0.5 -0.2 -0.7 -0.7 -0.7 0.9 0.8 0.4
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.8 0.3 -2.0 -1.7 -1.8 – -0.2 0.2 0.2 0.8 2.0 3.7 7.7 6.0 7.0
Cytoplasm 0.4 0.2 2.2 -0.2 1.0 – 1.0 1.1 2.2 1.0 0 -0.8 4.4 3.4 4.7
Endoplasmic Reticulum -1.5 -2.7 -4.6 -4.0 -10.1 – -6.0 -7.5 -9.6 -4.0 -2.0 -3.2 -15.6 -14.5 -14.2
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 3.6 4.8 2.8
Mitochondria 1.7 3.2 4.0 4.4 3.9 – 3.7 7.8 8.0 0 0 0 0.3 1.8 2.2
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1.2 0 – 0.3 1.1 0.3 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.3 3.6 7.7 – 4.6 2.8 4.1 3.9 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.1775 14.1982 12.2814 10.9139 10.3457 12.6465 12.6491 14.0763 12.7087 12.8378 11.2286 12.652 13.1255 13.086 12.1693 12.8858 11.0972 12.5187
Actin 0.0335 0.0045 0.0243 0.0406 0.0079 0.0008 0.0327 0.0001 0.0014 0.0007 0.0118 0.0012 0.0226 0.0002 0.0142 0.0001 0.0179 0.0012
Bud 0.0012 0.0001 0.0002 0.0006 0.0003 0.0001 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0
Bud Neck 0.0029 0.001 0.0006 0.0214 0.0114 0.0011 0.0006 0.0001 0.0003 0.0007 0.0002 0.0002 0.0002 0.0001 0.0016 0.0005 0.001 0.0004
Bud Periphery 0.0022 0.0002 0.0003 0.001 0.0007 0.0004 0.0007 0 0.0002 0.0001 0.0003 0.0001 0.0002 0 0.0003 0.0001 0.0002 0.0001
Bud Site 0.011 0.0028 0.002 0.0087 0.0009 0 0.0018 0.0001 0.0005 0 0.0007 0.0001 0.0003 0.0001 0.0007 0 0.0006 0
Cell Periphery 0.0034 0.009 0.0073 0.0012 0.0016 0.0021 0.0015 0.0037 0.0041 0.0012 0.0017 0.0009 0.0009 0.0053 0.0025 0.0015 0.0026 0.0014
Cytoplasm 0.0168 0.0387 0.0072 0.0014 0.0133 0.0108 0.0179 0.0083 0.0082 0.0251 0.0077 0.0052 0.0112 0.0095 0.0043 0.0013 0.0136 0.0048
Cytoplasmic Foci 0.0222 0.0062 0.0043 0.0232 0.0375 0.0006 0.0102 0.0002 0.0071 0.0008 0.0106 0.0016 0.0112 0.0001 0.0046 0 0.0122 0.0006
Eisosomes 0.0005 0.0002 0.0003 0.0011 0.0005 0.0001 0.0009 0 0.0001 0.0001 0.0013 0.0001 0.0006 0 0.0002 0 0.0001 0.0002
Endoplasmic Reticulum 0.7347 0.8876 0.8968 0.7059 0.7296 0.9388 0.8463 0.9631 0.9449 0.948 0.8012 0.9673 0.9021 0.9745 0.927 0.9893 0.8718 0.9742
Endosome 0.024 0.0051 0.0109 0.0164 0.0401 0.0047 0.0155 0.0003 0.0075 0.0045 0.048 0.0059 0.0055 0.0003 0.0041 0.0005 0.0109 0.007
Golgi 0.0074 0.0035 0.0092 0.0213 0.0137 0.0049 0.0078 0.0002 0.0057 0.004 0.0153 0.0019 0.0035 0.0004 0.0067 0.0001 0.0061 0.0009
Lipid Particles 0.0236 0.0192 0.0059 0.0363 0.0134 0.0023 0.0113 0.0204 0.0036 0.0099 0.0301 0.0043 0.0073 0.0037 0.0097 0.0001 0.0146 0.0008
Mitochondria 0.0177 0.0004 0.0033 0.0521 0.0982 0.0043 0.0072 0 0.0046 0.0002 0.0273 0.0022 0.0065 0 0.0053 0.0001 0.0018 0.0003
None 0.0034 0.0006 0.0087 0.0053 0.0013 0.0002 0.0101 0 0.0004 0.0001 0.0065 0.0003 0.0007 0 0.0019 0 0.0009 0.0003
Nuclear Periphery 0.0457 0.01 0.0071 0.0118 0.0065 0.0193 0.0177 0.0021 0.0032 0.0029 0.0166 0.0041 0.02 0.0039 0.0047 0.0046 0.0193 0.0053
Nucleolus 0.0013 0.0001 0.0011 0.0232 0.0005 0.0001 0.0007 0 0 0 0.0005 0.0001 0.0001 0 0.0004 0 0.0001 0
Nucleus 0.007 0.0015 0.0021 0.0029 0.0012 0.004 0.0076 0.0001 0.0002 0.0002 0.0016 0.0002 0.0007 0.0001 0.0004 0.0002 0.0008 0.0005
Peroxisomes 0.0099 0.0036 0.0026 0.0168 0.004 0.0001 0.0031 0 0.0025 0.0001 0.0053 0.0017 0.0032 0 0.0084 0 0.0119 0.0001
Punctate Nuclear 0.0152 0.0006 0.0024 0.0027 0.0134 0.0001 0.0022 0 0.0017 0.0002 0.0016 0.0009 0.0005 0 0.0009 0 0.0066 0.0003
Vacuole 0.0128 0.0049 0.0033 0.0029 0.0023 0.0036 0.0026 0.0011 0.003 0.001 0.0085 0.0014 0.002 0.0016 0.0018 0.0013 0.0059 0.0012
Vacuole Periphery 0.0035 0.0002 0.0004 0.0031 0.0018 0.0015 0.0013 0.0001 0.0005 0.0002 0.0031 0.0005 0.0007 0.0001 0.0005 0.0003 0.001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.9489 44.582 32.0479 33.3237 36.3715 35.8252 35.9206 36.6036 36.9729 45.2397
Translational Efficiency 2.5205 1.3736 1.6323 1.2978 1.6637 1.9671 1.6846 1.4461 1.5943 1.5155

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘