Standard name
Description Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.09 0.08 0.05 0.05 0.09 0.07 0.08 0.07 0.05 0.06 0.06 0.1 0.1 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.09 0.07 0.1 0.05 0.05 0.05 0.09 0.1 0.1 0.12 0.16 0.23 0.51 0.41 0.5 0 0 0 0 0 0
Cytoplasm 0.09 0.06 0.07 0.07 0.13 0.06 0.09 0.09 0.09 0.13 0.09 0.06 0.05 0.26 0.21 0.29 0.06 0 0 0 0 0
Endoplasmic Reticulum 0.9 0.94 0.89 0.84 0.76 0.79 0.46 0.69 0.59 0.49 0.78 0.88 0.83 0 0 0 0.86 0.9 0.96 0.91 0.89 0.87
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.21 0.11 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.13 0.17 0.18 0.16 0.15 0.38 0.39 0 0 0 0 0.09 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.07 0 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.09 0.32 0.14 0.05 0.11 0.1 0 0 0 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 10 27 28 26 30 49 41 31 35 14 32 34 14 11 8 0 0 0 1 2 5
Bud 0 0 2 2 5 8 10 6 7 6 1 0 1 0 1 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 2 13 20 34 24 36 29 51 36 51 31 91 141 72 46 47 0 1 2 0 3 5
Cytoplasm 6 9 21 23 65 39 50 53 35 69 24 37 29 36 23 27 1 3 0 1 11 15
Endoplasmic Reticulum 63 132 255 287 382 527 258 401 219 256 202 508 500 1 2 0 17 91 138 143 281 347
Endosome 0 0 0 1 0 0 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Golgi 0 3 12 3 1 2 3 3 0 0 1 11 12 19 23 10 0 0 0 1 1 1
Mitochondria 0 5 22 45 85 122 90 88 142 203 0 1 0 6 10 10 0 1 0 2 3 4
Nucleus 1 0 0 1 0 1 1 3 0 0 0 2 1 1 1 0 0 0 0 0 0 0
Nuclear Periphery 1 6 9 4 8 46 14 28 25 25 0 4 5 1 2 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 4 10 36 57 177 79 20 57 26 6 25 1 8 3 0 0 1 2 10 8
Unique Cell Count 70 141 286 340 502 666 557 585 369 525 259 580 603 140 111 94 21 102 145 158 317 397
Labelled Cell Count 75 178 372 438 632 868 689 755 516 703 299 692 748 151 127 106 21 102 145 158 317 397


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.9 14.3 14.3 12.4 10.8 10.0 9.9 9.7 8.9 8.8 15.5 16.1 15.4 17.9 18.3 18.6 17.2 17.2 17.1
Std Deviation (1e-4) 3.1 2.1 2.2 2.0 1.7 1.7 1.6 1.7 1.7 1.6 2.5 2.5 2.6 3.5 3.3 3.8 2.6 2.7 2.6
Intensity Change (Log2) 0.0 -0.21 -0.42 -0.52 -0.53 -0.56 -0.68 -0.71 0.11 0.16 0.11 0.32 0.35 0.38 0.26 0.26 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.8 0.4 -0.9 -1.3 0.7 0 0.5 -0.2 -0.7 -0.7 -0.7 0.9 0.8 0.4
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.8 0.3 -2.0 -1.7 -1.8 -0.2 0.2 0.2 0.8 2.0 3.7 7.7 6.0 7.0
Cytoplasm 0.4 0.2 2.2 -0.2 1.0 1.0 1.1 2.2 1.0 0 -0.8 4.4 3.4 4.7
Endoplasmic Reticulum -1.5 -2.7 -4.6 -4.0 -10.1 -6.0 -7.5 -9.6 -4.0 -2.0 -3.2 -15.6 -14.5 -14.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.6 4.8 2.8
Mitochondria 1.7 3.2 4.0 4.4 3.9 3.7 7.8 8.0 0 0 0 0.3 1.8 2.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1.2 0 0.3 1.1 0.3 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.3 3.6 7.7 4.6 2.8 4.1 3.9 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Get1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available