ORF
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.12 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.79 0.76 0.79 0.87 0.83 – 0.78 0.67 0.63 0.64 0.63 0.79 0.91 0.96 0.56 0.57 0.52 0.79 0.79 0.76 0.77 0.77 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05
Mitochondria 0 0 0.18 0.17 0.07 0.33 – 0.41 0.6 0.55 0.52 0.58 0 0 0 0 0.06 0 0 0 0.05 0 0 0
Nucleus 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.13 0 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07
Vac/Vac Membrane 0.12 0.17 0 0.11 0.09 0 – 0 0 0 0 0 0.23 0.08 0 0.31 0.32 0.3 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 2 7
Bud 0 0 2 4 3 4 – 7 7 8 4 6 2 1 2 0 0 0 5 3 1 4 21 42
Bud Neck 0 0 0 0 0 0 – 2 0 3 0 0 0 0 0 0 0 0 2 0 0 0 1 4
Bud Site 0 0 0 0 0 0 – 1 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 – 2 1 2 4 2 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 227 46 13 120 215 192 – 406 183 200 112 162 261 429 519 53 39 47 210 76 46 79 282 241
Endoplasmic Reticulum 2 0 0 0 2 0 – 0 0 1 0 3 1 1 1 1 1 4 0 0 0 0 1 3
Endosome 2 1 0 1 3 1 – 2 0 2 1 1 8 5 4 6 3 7 6 1 1 0 8 29
Golgi 1 0 0 1 0 0 – 0 0 1 0 0 1 0 0 3 2 6 3 0 0 0 2 22
Mitochondria 7 2 3 26 17 77 – 213 163 176 90 148 4 12 6 4 4 4 8 1 2 0 3 11
Nucleus 3 0 1 0 1 0 – 6 0 8 3 3 3 0 1 1 0 0 18 5 1 6 13 17
Nuclear Periphery 0 0 0 1 0 0 – 3 1 1 0 2 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 1 1 0 0 0 0 – 2 0 2 1 0 2 5 1 12 3 8 0 0 0 1 1 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 3 1 1 1 0 0 1 0 0 0 5
SpindlePole 0 0 0 0 0 0 – 5 2 1 1 2 1 1 1 1 0 1 3 1 1 6 10 30
Vac/Vac Membrane 32 10 0 17 23 2 – 16 3 5 7 3 75 37 15 29 22 27 5 2 2 4 13 19
Unique Cell Count 260 58 17 152 248 231 521 272 319 174 256 332 474 538 95 68 90 268 96 61 103 369 444
Labelled Cell Count 276 60 19 170 264 277 665 360 410 224 334 358 494 552 112 75 105 268 96 61 103 369 444


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.8 4.9 6.1 5.7 3.5 – 3.5 3.0 3.5 3.2 3.6 10.6 9.1 6.6 18.9 16.6 17.7 7.2 7.2 7.5
Std Deviation (1e-4) 1.7 2.0 1.5 3.3 1.8 1.2 – 1.4 1.3 1.8 1.6 8.6 3.6 3.9 1.8 13.9 7.5 9.2 3.4 2.2 2.3
Intensity Change (Log2) – – – 0.32 0.22 -0.47 – -0.48 -0.72 -0.48 -0.59 -0.44 1.11 0.9 0.44 1.96 1.77 1.86 0.56 0.55 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.2 0.7 – 0.1 -0.8 -1.1 -1.0 -1.1 0.2 1.9 4.1 -1.6 -1.4 -1.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0