Standard name
Description Isopropylmalate isomerase; catalyzes the second step in the leucine biosynthesis pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.1 0.11 0.13 0.08 0 0 0 0 0 0 0 0 0.13 0.25 0.36 0.32 0.48 0.21 0 0 0 0 0 0
Cytoplasm 0.96 0.92 0.94 0.97 0.99 0.99 0.99 0.95 0.96 0.89 0.88 0.9 0.94 0.96 0.77 0.78 0.64 0.84 0.91 0.91 0.93 0.89 0.75 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0.09 0 0 0 0 0.17 0.12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 11 18 8 4 1 1 3 1 4 1 6 9 14 8 71 86 30 6 4 0 0 0 1
Cytoplasm 118 89 130 92 155 120 182 175 92 115 108 130 66 54 17 173 115 122 141 139 157 73 95 90
Endoplasmic Reticulum 0 0 0 0 0 0 1 4 1 9 8 7 0 0 0 2 5 13 2 1 4 3 21 14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2 2 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Nucleolus 0 0 0 0 0 0 0 3 2 4 5 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 1 0 0 0 1 1 0 0 3
Unique Cell Count 123 97 138 95 157 121 184 184 96 129 123 145 70 56 22 221 181 145 155 153 170 82 128 120
Labelled Cell Count 130 100 148 100 160 121 184 185 96 132 126 146 75 68 25 249 208 166 155 153 170 82 128 120


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 558.6 533.9 446.9 415.0 376.4 309.2 268.7 246.9 230.8 203.1 186.5 192.5 529.6 471.7 511.5 591.5 636.3 575.8 434.6 488.8 500.8
Std Deviation (1e-4) 83.2 90.7 103.3 94.8 77.8 69.0 60.3 65.1 65.7 50.9 47.4 56.0 137.2 144.0 74.6 173.3 152.9 159.8 164.3 88.6 95.1
Intensity Change (Log2) – – – -0.11 -0.25 -0.53 -0.73 -0.86 -0.95 -1.14 -1.26 -1.21 0.24 0.08 0.19 0.4 0.51 0.37 -0.04 0.13 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.1 -3.4 -3.8 -4.7 -4.1 -3.3 -3.0 -3.8 -2.7 0 2.0 0 4.1 6.5 1.7
Cytoplasm 0.9 2.1 2.2 2.4 0.4 0.6 -1.5 -1.8 -1.4 0 0.6 -2.7 -4.0 -6.4 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 325.5561 616.2823 497.2635 583.1348 225.4459 544.9735 495.0411 840.4164 700.2923 741.5354 579.9003 688.723 454.1036 741.3732 623.3236 705.5496 321.5243 662.7188
Actin 0.0272 0.0001 0.0287 0.0001 0.001 0.0002 0.0054 0.0001 0.0002 0.0001 0.0001 0.0001 0.0229 0.0009 0.003 0 0.0907 0.0001
Bud 0.0058 0.0002 0.002 0.0001 0.0071 0.0004 0.0084 0.0002 0.0003 0 0.0001 0.0002 0.0071 0.0003 0.0009 0 0.0027 0.0001
Bud Neck 0.0009 0.0001 0.0005 0.0001 0.0001 0.0003 0.0007 0 0.0001 0.0001 0.0002 0.0002 0.0005 0.0001 0.0002 0.0001 0.0034 0.0002
Bud Periphery 0.0048 0 0.0011 0 0.0014 0 0.0017 0 0.0001 0 0 0 0.0019 0.0002 0.0004 0 0.0032 0
Bud Site 0.0072 0 0.0008 0 0.0012 0 0.0027 0 0.0001 0 0 0 0.0022 0.0003 0.0012 0 0.0038 0
Cell Periphery 0.0172 0.0001 0.0003 0 0.001 0.0001 0.0014 0 0.0002 0 0 0 0.0014 0.0001 0.0003 0 0.007 0
Cytoplasm 0.5765 0.9737 0.8565 0.8231 0.5258 0.8983 0.6722 0.9706 0.8377 0.8247 0.9623 0.9089 0.7175 0.9756 0.8624 0.9293 0.5417 0.9509
Cytoplasmic Foci 0.0478 0 0.0081 0 0.0316 0.0001 0.035 0 0.0169 0 0.0001 0.0001 0.026 0.0004 0.0214 0 0.0295 0
Eisosomes 0.0013 0 0.0002 0 0.0012 0 0.0012 0 0.0002 0 0 0 0.0016 0 0.0004 0 0.0014 0
Endoplasmic Reticulum 0.0049 0 0.0026 0 0.0002 0.0001 0.0023 0 0.0002 0.0001 0.0001 0 0.0102 0.0003 0.0021 0 0.0278 0
Endosome 0.0159 0 0.0135 0 0.0001 0 0.0053 0 0.0003 0 0 0 0.0143 0.0004 0.0059 0 0.072 0
Golgi 0.0054 0 0.0103 0 0.0001 0 0.0006 0 0 0 0 0 0.0043 0.0001 0.0076 0 0.0161 0
Lipid Particles 0.0084 0 0.0047 0 0.0009 0 0.002 0 0.0008 0 0 0 0.0049 0 0.0023 0 0.0119 0
Mitochondria 0.0274 0.0004 0.0127 0.0012 0.2514 0.0009 0.0527 0.0005 0.0303 0.0009 0.0003 0.0004 0.0324 0.0022 0.0147 0.0002 0.0362 0.0002
None 0.0195 0.0003 0.0076 0.0002 0.0979 0.0004 0.0316 0.0002 0.0244 0.0003 0.0002 0.0004 0.0222 0.0014 0.0175 0.0002 0.0673 0.0002
Nuclear Periphery 0.0377 0.0019 0.012 0.0033 0.0011 0.0066 0.0226 0.0008 0.0037 0.0065 0.0039 0.0031 0.0248 0.0017 0.0024 0.0066 0.0176 0.003
Nucleolus 0.0474 0.0001 0.0013 0.0006 0.0415 0.0003 0.0309 0.0001 0.0105 0 0.0001 0.0001 0.0334 0.0019 0.0094 0.0002 0.0237 0
Nucleus 0.0953 0.0228 0.029 0.1707 0.0219 0.0912 0.1092 0.0272 0.071 0.1671 0.0321 0.0861 0.0615 0.0137 0.0437 0.063 0.0187 0.045
Peroxisomes 0.0047 0 0.0011 0 0.0053 0 0.0021 0 0.0005 0 0 0 0.0018 0 0.0011 0 0.0024 0
Punctate Nuclear 0.0219 0.0001 0.001 0.0001 0.0008 0.0002 0.0044 0 0.001 0.0002 0.0001 0.0002 0.0024 0.0001 0.0006 0.0001 0.0019 0.0001
Vacuole 0.0184 0.0001 0.0038 0.0001 0.0036 0.0004 0.0034 0.0001 0.0005 0 0.0001 0.0001 0.004 0.0002 0.0014 0.0001 0.0123 0.0001
Vacuole Periphery 0.0043 0 0.0023 0.0002 0.0048 0.0004 0.0042 0.0001 0.0009 0.0001 0.0001 0.0001 0.0028 0.0001 0.0014 0.0001 0.0088 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 122.3647 86.8348 68.5015 69.2435 69.6965 77.7611 66.8079 59.1143 56.3838 61.6959
Translational Efficiency 1.0318 1.3836 1.2407 1.3744 1.4323 1.2425 1.1607 1.0483 1.0737 1.06

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘